{"id":"https://openalex.org/W4286856689","doi":"https://doi.org/10.3791/1852-v","title":"Isolation and Enrichment of Rat Mesenchymal Stem Cells (MSCs) and Separation of Single-colony Derived MSCs","display_name":"Isolation and Enrichment of Rat Mesenchymal Stem Cells (MSCs) and Separation of Single-colony Derived MSCs","publication_year":2010,"publication_date":"2010-03-22","ids":{"openalex":"https://openalex.org/W4286856689","doi":"https://doi.org/10.3791/1852-v"},"language":"en","primary_location":{"id":"doi:10.3791/1852-v","doi":"https://doi.org/10.3791/1852-v","is_oa":false,"landing_page_url":"https://doi.org/10.3791/1852-v","pdf_url":null,"source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5101982027","display_name":"Linxia Zhang","orcid":"https://orcid.org/0000-0002-5406-9973","observed_orcids":["https://orcid.org/0000-0002-5406-9973"]},"institutions":[{"id":"https://openalex.org/I1301076528","display_name":"City Of Hope National Medical Center","ror":"https://ror.org/00w6g5w60","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1301076528"]},{"id":"https://openalex.org/I4210110459","display_name":"City of Hope","ror":"https://ror.org/01z1vct10","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1301076528","https://openalex.org/I4210110459"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Linxia Zhang","raw_affiliation_strings":["Department of Chemical Engineering and Materials Science, City of Hope Cancer Center"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical Engineering and Materials Science, City of Hope Cancer Center","institution_ids":["https://openalex.org/I1301076528","https://openalex.org/I4210110459"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5102757744","display_name":"Christina Chan","orcid":"https://orcid.org/0000-0003-3054-2727","observed_orcids":["https://orcid.org/0000-0003-3054-2727","https://orcid.org/0009-0003-7693-8206"]},"institutions":[{"id":"https://openalex.org/I1301076528","display_name":"City Of Hope National Medical Center","ror":"https://ror.org/00w6g5w60","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1301076528"]},{"id":"https://openalex.org/I4210110459","display_name":"City of Hope","ror":"https://ror.org/01z1vct10","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1301076528","https://openalex.org/I4210110459"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Christina Chan","raw_affiliation_strings":["Department of Chemical Engineering and Materials Science, City of Hope Cancer Center"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical Engineering and Materials Science, City of Hope Cancer Center","institution_ids":["https://openalex.org/I1301076528","https://openalex.org/I4210110459"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.1412,"has_fulltext":false,"cited_by_count":45,"citation_normalized_percentile":{"value":0.59272141,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":99},"biblio":{"volume":null,"issue":"37","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10176","display_name":"Mesenchymal stem cell research","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2716","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10176","display_name":"Mesenchymal stem cell research","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2716","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10345","display_name":"Hematopoietic Stem Cell Transplantation","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/mesenchymal-stem-cells","display_name":"mesenchymal stem cells","score":0.996999979019165},{"id":"https://openalex.org/keywords/rats","display_name":"rats","score":0.7739999890327454},{"id":"https://openalex.org/keywords/bone-marrow","display_name":"bone marrow","score":0.8349999785423279},{"id":"https://openalex.org/keywords/magnetic-activated-cell-sorting","display_name":"magnetic cell separation","score":0.6919999718666077},{"id":"https://openalex.org/keywords/icam-1","display_name":"ICAM-1","score":0.9729999899864197},{"id":"https://openalex.org/keywords/cd90","display_name":"CD90","score":1.0},{"id":"https://openalex.org/keywords/hematopoietic-stem-cells","display_name":"hematopoietic stem cells","score":0.9039999842643738},{"id":"https://openalex.org/keywords/cd45","display_name":"CD45","score":0.7739999890327454},{"id":"https://openalex.org/keywords/cell-isolation","display_name":"cell isolation","score":0.6449999809265137},{"id":"https://openalex.org/keywords/cell-enrichment","display_name":"cell enrichment","score":0.6940000057220459},{"id":"https://openalex.org/keywords/stem-cell-heterogeneity","display_name":"stem cell heterogeneity","score":0.6909999847412109},{"id":"https://openalex.org/keywords/cell-surface-markers","display_name":"cell surface markers","score":0.6010000109672546}],"concepts":[{"id":"https://openalex.org/C198826908","wikidata":"https://www.wikidata.org/wiki/Q1922379","display_name":"Mesenchymal stem cell","level":2,"score":0.9303643107414246},{"id":"https://openalex.org/C2775941552","wikidata":"https://www.wikidata.org/wiki/Q25212305","display_name":"Isolation (microbiology)","level":2,"score":0.6061335206031799},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5176171064376831},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4945506751537323},{"id":"https://openalex.org/C28328180","wikidata":"https://www.wikidata.org/wiki/Q48196","display_name":"Stem cell","level":2,"score":0.42688149213790894},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.32819586992263794},{"id":"https://openalex.org/C60644358","wikidata":"https://www.wikidata.org/wiki/Q128570","display_name":"Bioinformatics","level":1,"score":0.1116417944431305}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.3791/1852-v","doi":"https://doi.org/10.3791/1852-v","is_oa":false,"landing_page_url":"https://doi.org/10.3791/1852-v","pdf_url":null,"source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.6240000128746033,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W3025892301","https://openalex.org/W4399176951","https://openalex.org/W1764066238","https://openalex.org/W2367185064","https://openalex.org/W3031557536","https://openalex.org/W1967764991","https://openalex.org/W2356769330","https://openalex.org/W2084241194","https://openalex.org/W2725594000","https://openalex.org/W148036350"],"abstract_inverted_index":{"MSCs":[0,47,61,96,101,125],"are":[1,102,107],"a":[2,10],"population":[3],"of":[4,72,99,109],"adult":[5],"stem":[6,33],"cells":[7,17,34,55,110,160],"that":[8,111],"is":[9,149],"promising":[11],"source":[12],"for":[13,135],"therapeutic":[14],"applications.":[15],"These":[16,119],"can":[18,26,165],"be":[19,27,167],"isolated":[20,54],"from":[21,30,48,162],"the":[22,31,94,123,136,146,163],"bone":[23],"marrow":[24],"and":[25,51,65,76,116,127,169],"easily":[28],"separated":[29],"hematopoietic":[32],"(HSCs)":[35],"due":[36],"to":[37,45,91,143,153],"their":[38],"plastic":[39],"adherence.":[40],"This":[41],"protocol":[42],"describes":[43],"how":[44],"isolate":[46],"rat":[49],"femurs":[50],"tibias.":[52],"The":[53,159],"were":[56,78,97],"further":[57],"enriched":[58],"against":[59],"two":[60],"surface":[62,73],"markers":[63,74,126],"CD54":[64,75],"CD90":[66,77],"by":[67,81],"magnetic":[68],"cell":[69],"sorting.":[70],"Expression":[71],"then":[79,166],"confirmed":[80],"flow":[82],"cytometry":[83],"analysis.":[84],"HSC":[85],"marker":[86,130],"CD45":[87],"was":[88],"also":[89],"included":[90],"check":[92],"if":[93],"sorted":[95],"depleted":[98],"HSCs.":[100],"naturally":[103],"quite":[104],"heterogeneous.":[105],"There":[106],"subpopulations":[108,120,148],"have":[112],"different":[113,137,147],"shapes,":[114],"proliferation":[115],"differentiation":[117],"abilities.":[118],"all":[121],"express":[122],"known":[124],"no":[128],"unique":[129],"has":[131],"yet":[132],"been":[133],"identified":[134],"subpopulations.":[138],"Therefore,":[139],"an":[140],"alternative":[141],"approach":[142],"separate":[144,154],"out":[145,155],"using":[150],"cloning":[151],"cylinders":[152],"single-colony":[156],"derived":[157,161],"cells.":[158],"single-colonies":[164],"cultured":[168],"evaluated":[170],"separately.":[171]},"counts_by_year":[{"year":2025,"cited_by_count":4},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":4},{"year":2022,"cited_by_count":6},{"year":2020,"cited_by_count":6},{"year":2019,"cited_by_count":9},{"year":2018,"cited_by_count":8},{"year":2017,"cited_by_count":2},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":2}],"updated_date":"2026-11-03T10:50:19.383937","created_date":"2022-07-25T00:00:00"}
