{"id":"https://openalex.org/W4286853923","doi":"https://doi.org/10.3791/54677-v","title":"Assessing Retinal Microglial Phagocytic Function In Vivo Using a Flow Cytometry-based Assay","display_name":"Assessing Retinal Microglial Phagocytic Function In Vivo Using a Flow Cytometry-based Assay","publication_year":2016,"publication_date":"2016-10-18","ids":{"openalex":"https://openalex.org/W4286853923","doi":"https://doi.org/10.3791/54677-v"},"language":"en","primary_location":{"id":"doi:10.3791/54677-v","doi":"https://doi.org/10.3791/54677-v","is_oa":false,"landing_page_url":"https://doi.org/10.3791/54677-v","pdf_url":null,"source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5024914580","display_name":"Salome Murinello","orcid":"https://orcid.org/0000-0002-9694-2160","observed_orcids":["https://orcid.org/0000-0002-9694-2160"]},"institutions":[{"id":"https://openalex.org/I123431417","display_name":"Scripps Research Institute","ror":"https://ror.org/02dxx6824","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I123431417"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Salome Murinello","raw_affiliation_strings":["Department of Cell and Molecular Biology, The Scripps Research Institute"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell and Molecular Biology, The Scripps Research Institute","institution_ids":["https://openalex.org/I123431417"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101198632","display_name":"Stacey K. Moreno","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I123431417","display_name":"Scripps Research Institute","ror":"https://ror.org/02dxx6824","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I123431417"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Stacey K. Moreno","raw_affiliation_strings":["Department of Cell and Molecular Biology, The Scripps Research Institute"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell and Molecular Biology, The Scripps Research Institute","institution_ids":["https://openalex.org/I123431417"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5087354201","display_name":"Matthew Scott Macauley","orcid":"https://orcid.org/0000-0003-4579-1048","observed_orcids":["https://orcid.org/0000-0003-4579-1048"]},"institutions":[{"id":"https://openalex.org/I123431417","display_name":"Scripps Research Institute","ror":"https://ror.org/02dxx6824","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I123431417"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Matthew S. Macauley","raw_affiliation_strings":["Department of Chemical Physiology, The Scripps Research Institute"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical Physiology, The Scripps Research Institute","institution_ids":["https://openalex.org/I123431417"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5078571293","display_name":"Susumu Sakimoto","orcid":"https://orcid.org/0000-0003-2603-1043","observed_orcids":["https://orcid.org/0000-0003-2603-1043"]},"institutions":[{"id":"https://openalex.org/I123431417","display_name":"Scripps Research Institute","ror":"https://ror.org/02dxx6824","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I123431417"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Susumu Sakimoto","raw_affiliation_strings":["Department of Cell and Molecular Biology, The Scripps Research Institute"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell and Molecular Biology, The Scripps Research Institute","institution_ids":["https://openalex.org/I123431417"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5022101366","display_name":"Peter D. Westenskow","orcid":"https://orcid.org/0000-0001-9841-6220","observed_orcids":["https://orcid.org/0000-0001-9841-6220"]},"institutions":[{"id":"https://openalex.org/I123431417","display_name":"Scripps Research Institute","ror":"https://ror.org/02dxx6824","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I123431417"]},{"id":"https://openalex.org/I4210108657","display_name":"The Lowy Medical Research Institute","ror":"https://ror.org/01jgzvw27","country_code":"US","type":"facility","lineage":["https://openalex.org/I4210108657"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Peter D. Westenskow","raw_affiliation_strings":["Department of Cell and Molecular Biology, The Scripps Research Institute","The Lowy Medical Research Institute"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell and Molecular Biology, The Scripps Research Institute","institution_ids":["https://openalex.org/I123431417"]},{"raw_affiliation_string":"The Lowy Medical Research Institute","institution_ids":["https://openalex.org/I4210108657"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5040174959","display_name":"Martin Friedlander","orcid":"https://orcid.org/0000-0003-4238-9651","observed_orcids":["https://orcid.org/0000-0003-4238-9651"]},"institutions":[{"id":"https://openalex.org/I123431417","display_name":"Scripps Research Institute","ror":"https://ror.org/02dxx6824","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I123431417"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Martin Friedlander","raw_affiliation_strings":["Department of Cell and Molecular Biology, The Scripps Research Institute"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell and Molecular Biology, The Scripps Research Institute","institution_ids":["https://openalex.org/I123431417"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":2,"citation_normalized_percentile":{"value":0.35819772,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":null,"issue":"116","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11266","display_name":"Neuroinflammation and Neurodegeneration Mechanisms","score":0.8051999807357788,"subfield":{"id":"https://openalex.org/subfields/2808","display_name":"Neurology"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11266","display_name":"Neuroinflammation and Neurodegeneration Mechanisms","score":0.8051999807357788,"subfield":{"id":"https://openalex.org/subfields/2808","display_name":"Neurology"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12672","display_name":"Phagocytosis and Immune Regulation","score":0.07240000367164612,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11438","display_name":"Retinal Imaging and Analysis","score":0.05400000140070915,"subfield":{"id":"https://openalex.org/subfields/2741","display_name":"Radiology, Nuclear Medicine and Imaging"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/retinal-microglia","display_name":"retinal microglia","score":0.9980000257492065},{"id":"https://openalex.org/keywords/microglial-phagocytosis","display_name":"microglial phagocytosis","score":0.9779999852180481},{"id":"https://openalex.org/keywords/flow-cytometry","display_name":"flow cytometry","score":0.6230000257492065},{"id":"https://openalex.org/keywords/age-related-macular-degeneration","display_name":"age-related macular degeneration","score":0.8029999732971191},{"id":"https://openalex.org/keywords/alzheimer-s-disease","display_name":"Alzheimer's disease","score":0.9629999995231628},{"id":"https://openalex.org/keywords/amyloid-plaques","display_name":"amyloid plaques","score":0.7850000262260437},{"id":"https://openalex.org/keywords/drusen","display_name":"drusen","score":0.9309999942779541},{"id":"https://openalex.org/keywords/rodent-models","display_name":"rodent models","score":0.35100001096725464}],"concepts":[{"id":"https://openalex.org/C2779830541","wikidata":"https://www.wikidata.org/wiki/Q1622829","display_name":"Microglia","level":3,"score":0.9318002462387085},{"id":"https://openalex.org/C160448771","wikidata":"https://www.wikidata.org/wiki/Q184726","display_name":"Phagocytosis","level":2,"score":0.8436757326126099},{"id":"https://openalex.org/C207001950","wikidata":"https://www.wikidata.org/wiki/Q141124","display_name":"In vivo","level":2,"score":0.6415671110153198},{"id":"https://openalex.org/C553184892","wikidata":"https://www.wikidata.org/wiki/Q1141429","display_name":"Flow cytometry","level":2,"score":0.6379445195198059},{"id":"https://openalex.org/C4224716","wikidata":"https://www.wikidata.org/wiki/Q10859258","display_name":"Immunostaining","level":3,"score":0.6370524168014526},{"id":"https://openalex.org/C2780827179","wikidata":"https://www.wikidata.org/wiki/Q422001","display_name":"Retinal","level":2,"score":0.5917822122573853},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5601788759231567},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.5549307465553284},{"id":"https://openalex.org/C2776403814","wikidata":"https://www.wikidata.org/wiki/Q27429789","display_name":"Macular degeneration","level":2,"score":0.5372018814086914},{"id":"https://openalex.org/C2777093970","wikidata":"https://www.wikidata.org/wiki/Q169342","display_name":"Retina","level":2,"score":0.5291959047317505},{"id":"https://openalex.org/C169760540","wikidata":"https://www.wikidata.org/wiki/Q207011","display_name":"Neuroscience","level":1,"score":0.48705413937568665},{"id":"https://openalex.org/C2780339063","wikidata":"https://www.wikidata.org/wiki/Q5201367","display_name":"Cytometry","level":3,"score":0.45180124044418335},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.36462998390197754},{"id":"https://openalex.org/C204232928","wikidata":"https://www.wikidata.org/wiki/Q899285","display_name":"Immunohistochemistry","level":2,"score":0.30661383271217346},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.3037126660346985},{"id":"https://openalex.org/C2776914184","wikidata":"https://www.wikidata.org/wiki/Q101991","display_name":"Inflammation","level":2,"score":0.2910381257534027},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.27310577034950256},{"id":"https://openalex.org/C118487528","wikidata":"https://www.wikidata.org/wiki/Q161437","display_name":"Ophthalmology","level":1,"score":0.09013834595680237},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.0},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.3791/54677-v","doi":"https://doi.org/10.3791/54677-v","is_oa":false,"landing_page_url":"https://doi.org/10.3791/54677-v","pdf_url":null,"source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.8371999859809875}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4412802690","https://openalex.org/W7202296112","https://openalex.org/W4313837205","https://openalex.org/W3118666849","https://openalex.org/W1977005041","https://openalex.org/W3037176027","https://openalex.org/W2407606996","https://openalex.org/W2992067369","https://openalex.org/W3134467461","https://openalex.org/W2966876117"],"abstract_inverted_index":{"Microglia":[0],"are":[1],"the":[2,7,41,84,119,174],"tissue":[3,132],"resident":[4],"macrophages":[5],"of":[6,17,25,43,89,111,142],"central":[8],"nervous":[9],"system":[10],"(CNS)":[11],"and":[12,48,55,82,99,135],"they":[13],"perform":[14],"a":[15,74],"variety":[16],"functions":[18],"that":[19,129],"support":[20],"CNS":[21],"homeostasis,":[22],"including":[23],"phagocytosis":[24,63,164],"damaged":[26],"synapses":[27],"or":[28],"cells,":[29],"debris,":[30],"and/or":[31],"invading":[32],"pathogens.":[33],"Impaired":[34],"phagocytic":[35,87,144],"function":[36,145],"has":[37,64],"been":[38,65],"implicated":[39],"in":[40,69,85,121,146,165,173],"pathogenesis":[42],"diseases":[44],"such":[45],"as":[46],"Alzheimer's":[47],"age-related":[49],"macular":[50],"degeneration,":[51],"where":[52],"amyloid-\u03b2":[53],"plaque":[54],"drusen":[56],"accumulate,":[57],"respectively.":[58],"Despite":[59],"its":[60,176],"importance,":[61],"microglial":[62,109,163],"challenging":[66],"to":[67,107,118,157,181],"assess":[68],"vivo.":[70],"Here,":[71],"we":[72],"describe":[73],"simple,":[75],"yet":[76],"robust,":[77],"technique":[78,170],"for":[79,138],"precisely":[80],"monitoring":[81],"quantifying":[83],"vivo":[86],"potential":[88],"retinal":[90],"microglia.":[91],"Previous":[92],"methods":[93],"have":[94],"relied":[95],"on":[96],"immunohistochemical":[97],"staining":[98],"imaging":[100],"techniques.":[101],"Our":[102],"method":[103,125],"uses":[104],"flow":[105],"cytometry":[106],"measure":[108],"uptake":[110],"fluorescently":[112],"labeled":[113],"particles":[114],"after":[115],"intravitreal":[116],"delivery":[117],"eye":[120],"live":[122],"rodents.":[123],"This":[124,151],"replaces":[126],"conventional":[127],"practices":[128],"involve":[130],"laborious":[131],"sectioning,":[133],"immunostaining,":[134],"imaging,":[136],"allowing":[137],"more":[139],"precise":[140],"quantification":[141],"microglia":[143],"just":[147],"under":[148],"six":[149],"hours.":[150],"procedure":[152],"can":[153],"also":[154],"be":[155],"adapted":[156],"test":[158],"how":[159],"various":[160],"compounds":[161],"alter":[162],"physiological":[166],"settings.":[167],"While":[168],"this":[169],"was":[171],"developed":[172],"eye,":[175],"use":[177],"is":[178],"not":[179],"limited":[180],"vision":[182],"research.":[183]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2024,"cited_by_count":1}],"updated_date":"2026-09-22T06:50:58.402377","created_date":"2022-07-25T00:00:00"}
