{"id":"https://openalex.org/W4286853760","doi":"https://doi.org/10.3791/2544-v","title":"Methods for Rapid Transfer and Localization of Lyme Disease Pathogens Within the Tick Gut","display_name":"Methods for Rapid Transfer and Localization of Lyme Disease Pathogens Within the Tick Gut","publication_year":2011,"publication_date":"2011-02-14","ids":{"openalex":"https://openalex.org/W4286853760","doi":"https://doi.org/10.3791/2544-v"},"language":"en","primary_location":{"id":"doi:10.3791/2544-v","doi":"https://doi.org/10.3791/2544-v","is_oa":false,"landing_page_url":"https://doi.org/10.3791/2544-v","pdf_url":null,"source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5046794778","display_name":"Toru Kariu","orcid":"https://orcid.org/0009-0005-8999-1043","observed_orcids":["https://orcid.org/0009-0005-8999-1043"]},"institutions":[{"id":"https://openalex.org/I66946132","display_name":"University of Maryland, College Park","ror":"https://ror.org/047s2c258","country_code":"US","type":"education","lineage":["https://openalex.org/I66946132"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Toru Kariu","raw_affiliation_strings":["Department of Veterinary Medicine, University of Maryland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Veterinary Medicine, University of Maryland","institution_ids":["https://openalex.org/I66946132"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5043207041","display_name":"Adam Steven Coleman","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I66946132","display_name":"University of Maryland, College Park","ror":"https://ror.org/047s2c258","country_code":"US","type":"education","lineage":["https://openalex.org/I66946132"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Adam S. Coleman","raw_affiliation_strings":["Department of Veterinary Medicine, University of Maryland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Veterinary Medicine, University of Maryland","institution_ids":["https://openalex.org/I66946132"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5055991332","display_name":"John F. Anderson","orcid":"https://orcid.org/0000-0003-0199-8469","observed_orcids":["https://orcid.org/0000-0003-0199-8469"]},"institutions":[{"id":"https://openalex.org/I2799945547","display_name":"Connecticut Agricultural Experiment Station","ror":"https://ror.org/02t7c5797","country_code":"US","type":"government","lineage":["https://openalex.org/I2799945547"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"John F. Anderson","raw_affiliation_strings":["Department of Entomology, Connecticut Agricultural Experiment Station"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Entomology, Connecticut Agricultural Experiment Station","institution_ids":["https://openalex.org/I2799945547"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5052457700","display_name":"Utpal Pal","orcid":"https://orcid.org/0000-0002-7504-4628","observed_orcids":["https://orcid.org/0000-0002-7504-4628"]},"institutions":[{"id":"https://openalex.org/I66946132","display_name":"University of Maryland, College Park","ror":"https://ror.org/047s2c258","country_code":"US","type":"education","lineage":["https://openalex.org/I66946132"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Utpal Pal","raw_affiliation_strings":["Department of Veterinary Medicine, University of Maryland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Veterinary Medicine, University of Maryland","institution_ids":["https://openalex.org/I66946132"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.9907,"has_fulltext":false,"cited_by_count":11,"citation_normalized_percentile":{"value":0.68850624,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":null,"issue":"48","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10296","display_name":"Vector-borne infectious diseases","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2405","display_name":"Parasitology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10296","display_name":"Vector-borne infectious diseases","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2405","display_name":"Parasitology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12134","display_name":"Insect symbiosis and bacterial influences","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1109","display_name":"Insect Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12361","display_name":"Study of Mite Species","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1105","display_name":"Ecology, Evolution, Behavior and Systematics"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/lyme-disease","display_name":"Lyme disease","score":0.9760000109672546},{"id":"https://openalex.org/keywords/borrelia-burgdorferi","display_name":"Borrelia burgdorferi","score":1.0},{"id":"https://openalex.org/keywords/tick-infection","display_name":"tick infection","score":0.6639999747276306},{"id":"https://openalex.org/keywords/microinjection","display_name":"microinjection","score":0.8730000257492065},{"id":"https://openalex.org/keywords/nymphal-ticks","display_name":"nymphal ticks","score":0.902999997138977}],"concepts":[{"id":"https://openalex.org/C2779159148","wikidata":"https://www.wikidata.org/wiki/Q2723634","display_name":"Borrelia burgdorferi","level":3,"score":0.9063970446586609},{"id":"https://openalex.org/C2777553296","wikidata":"https://www.wikidata.org/wiki/Q201989","display_name":"Lyme disease","level":2,"score":0.8606205582618713},{"id":"https://openalex.org/C2779620486","wikidata":"https://www.wikidata.org/wiki/Q10304508","display_name":"Tick","level":2,"score":0.7647674083709717},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7159977555274963},{"id":"https://openalex.org/C2776460866","wikidata":"https://www.wikidata.org/wiki/Q170065","display_name":"Pathogen","level":2,"score":0.6628077626228333},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.584279477596283},{"id":"https://openalex.org/C2776397665","wikidata":"https://www.wikidata.org/wiki/Q1951212","display_name":"Ixodes","level":3,"score":0.49557578563690186},{"id":"https://openalex.org/C2776005033","wikidata":"https://www.wikidata.org/wiki/Q184243","display_name":"Tick-borne disease","level":3,"score":0.45217397809028625},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.44245487451553345},{"id":"https://openalex.org/C62525196","wikidata":"https://www.wikidata.org/wiki/Q3311945","display_name":"Microinjection","level":2,"score":0.4422052800655365},{"id":"https://openalex.org/C2780508971","wikidata":"https://www.wikidata.org/wiki/Q115528","display_name":"Borrelia","level":4,"score":0.41907137632369995},{"id":"https://openalex.org/C2776127032","wikidata":"https://www.wikidata.org/wiki/Q1429304","display_name":"Ixodidae","level":3,"score":0.4117191731929779},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.2726327180862427},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.21964120864868164},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.3791/2544-v","doi":"https://doi.org/10.3791/2544-v","is_oa":false,"landing_page_url":"https://doi.org/10.3791/2544-v","pdf_url":null,"source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.4043000042438507,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2764178444","https://openalex.org/W2121033175","https://openalex.org/W4401962142","https://openalex.org/W3082674303","https://openalex.org/W2142330717","https://openalex.org/W2587546846","https://openalex.org/W1989579711","https://openalex.org/W2140495367","https://openalex.org/W7135710017","https://openalex.org/W2322896760"],"abstract_inverted_index":{"Lyme":[0,33,86,235],"disease":[1,34,87,236],"is":[2,13,78,115,168],"caused":[3],"by":[4,17,47,126,182],"infection":[5,20,71,137,172],"with":[6,44,158,221],"the":[7,36,53,83,102,119,127,216,239],"spirochete":[8],"pathogen":[9,145],"Borrelia":[10],"burgdorferi,":[11],"which":[12],"maintained":[14],"in":[15,35,109,142,162,173,222,238],"nature":[16],"a":[18,75,110,193,230],"tick-rodent":[19],"cycle":[21],"1.":[22],"A":[23],"tick-borne":[24],"murine":[25],"model":[26],"2":[27],"has":[28],"been":[29],"developed":[30],"to":[31,59,61,94,134,155,170,191],"study":[32,84],"laboratory.":[37],"While":[38],"na\u00edve":[39],"ticks":[40,97,125,157,183,220],"can":[41,152,177,187],"be":[42,153,179,189],"infected":[43,51,96,124],"B.":[45,122,159,224],"burgdorferi":[46,123,160],"feeding":[48,129],"them":[49],"on":[50],"mice,":[52],"molting":[54],"process":[55,130],"takes":[56],"several":[57],"weeks":[58],"months":[60],"complete.":[62],"Therefore,":[63],"development":[64],"of":[65,85,104,107,112,121,144,195,218,234],"more":[66],"rapid":[67],"and":[68,98,139,175],"efficient":[69],"tick":[70,240],"techniques,":[72],"such":[73],"as":[74,118],"microinjection-based":[76],"procedure,":[77],"an":[79,165],"important":[80,117],"tool":[81],"for":[82,201,232],"3,4.":[88],"The":[89],"procedure":[90],"requires":[91],"only":[92],"hours":[93],"generate":[95],"allows":[99],"control":[100],"over":[101],"delivery":[103],"equal":[105],"quantities":[106],"spirochetes":[108],"cohort":[111],"ticks.":[113,149],"This":[114,185],"particularly":[116],"generation":[120],"natural":[128],"using":[131,242],"mice":[132,174],"fails":[133],"ensure":[135],"100%":[136],"rate":[138],"potentially":[140],"results":[141],"variation":[143],"burden":[146],"amongst":[147],"fed":[148],"Furthermore,":[150],"microinjection":[151,217],"used":[154,190],"infect":[156],"isolates":[161],"cases":[163],"where":[164],"attenuated":[166],"strain":[167],"unable":[169],"establish":[171],"thus":[176],"not":[178],"naturally":[180],"acquired":[181],"5.":[184],"technique":[186],"also":[188,228],"deliver":[192],"variety":[194],"other":[196],"biological":[197],"materials":[198],"into":[199],"ticks,":[200],"example,":[202],"specific":[203],"antibodies":[204],"or":[205],"double":[206],"stranded":[207],"RNA":[208],"6.":[209],"In":[210],"this":[211],"article,":[212],"we":[213],"will":[214,227],"demonstrate":[215],"nymphal":[219],"vitro-grown":[223],"burgdorferi.":[225],"We":[226],"describe":[229],"method":[231],"localization":[233],"pathogens":[237],"gut":[241],"confocal":[243],"immunofluorescence":[244],"microscopy.":[245]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2016,"cited_by_count":2},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":3}],"updated_date":"2026-09-29T14:09:16.559479","created_date":"2022-07-25T00:00:00"}
