{"id":"https://openalex.org/W4286853285","doi":"https://doi.org/10.3791/52075-v","title":"Porous Silicon Microparticles for Delivery of siRNA Therapeutics","display_name":"Porous Silicon Microparticles for Delivery of siRNA Therapeutics","publication_year":2015,"publication_date":"2015-01-15","ids":{"openalex":"https://openalex.org/W4286853285","doi":"https://doi.org/10.3791/52075-v"},"language":"en","primary_location":{"id":"doi:10.3791/52075-v","doi":"https://doi.org/10.3791/52075-v","is_oa":false,"landing_page_url":"https://doi.org/10.3791/52075-v","pdf_url":null,"source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5074548953","display_name":"Jianliang Shen","orcid":"https://orcid.org/0000-0003-4351-4872","observed_orcids":["https://orcid.org/0000-0003-4351-4872"]},"institutions":[{"id":"https://openalex.org/I1295876152","display_name":"Houston Methodist","ror":"https://ror.org/027zt9171","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1295876152"]},{"id":"https://openalex.org/I157773358","display_name":"Sun Yat-sen University","ror":"https://ror.org/0064kty71","country_code":"CN","type":"education","lineage":["https://openalex.org/I157773358"]},{"id":"https://openalex.org/I4405286254","display_name":"Houston Methodist Research Institute","ror":"https://ror.org/01mcrxx35","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I1295876152","https://openalex.org/I4405286254"]}],"countries":["CN","US"],"is_corresponding":false,"raw_author_name":"Jianliang Shen","raw_affiliation_strings":["Department of Nanomedicine, Houston Methodist Research Institute","MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-sen University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Nanomedicine, Houston Methodist Research Institute","institution_ids":["https://openalex.org/I1295876152","https://openalex.org/I4405286254"]},{"raw_affiliation_string":"MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-sen University","institution_ids":["https://openalex.org/I157773358"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100639319","display_name":"Xiaoyan Wu","orcid":"https://orcid.org/0000-0001-5086-1712","observed_orcids":["https://orcid.org/0000-0001-5086-1712"]},"institutions":[{"id":"https://openalex.org/I1295876152","display_name":"Houston Methodist","ror":"https://ror.org/027zt9171","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1295876152"]},{"id":"https://openalex.org/I4405286254","display_name":"Houston Methodist Research Institute","ror":"https://ror.org/01mcrxx35","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I1295876152","https://openalex.org/I4405286254"]},{"id":"https://openalex.org/I47720641","display_name":"Huazhong University of Science and Technology","ror":"https://ror.org/00p991c53","country_code":"CN","type":"education","lineage":["https://openalex.org/I47720641"]}],"countries":["CN","US"],"is_corresponding":false,"raw_author_name":"Xiaoyan Wu","raw_affiliation_strings":["Department of Nanomedicine, Houston Methodist Research Institute","Pediatrics Department of Union Hospital, Huazhong University of Science and Technology"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Nanomedicine, Houston Methodist Research Institute","institution_ids":["https://openalex.org/I1295876152","https://openalex.org/I4405286254"]},{"raw_affiliation_string":"Pediatrics Department of Union Hospital, Huazhong University of Science and Technology","institution_ids":["https://openalex.org/I47720641"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5003528434","display_name":"Yeon\u2010Ju Lee","orcid":"https://orcid.org/0000-0003-4814-943X","observed_orcids":["https://orcid.org/0000-0003-4814-943X"]},"institutions":[{"id":"https://openalex.org/I1295876152","display_name":"Houston Methodist","ror":"https://ror.org/027zt9171","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1295876152"]},{"id":"https://openalex.org/I4405286254","display_name":"Houston Methodist Research Institute","ror":"https://ror.org/01mcrxx35","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I1295876152","https://openalex.org/I4405286254"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yeonju Lee","raw_affiliation_strings":["Department of Nanomedicine, Houston Methodist Research Institute"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Nanomedicine, Houston Methodist Research Institute","institution_ids":["https://openalex.org/I1295876152","https://openalex.org/I4405286254"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5075780868","display_name":"Joy Wolfram","orcid":"https://orcid.org/0000-0003-0579-9897","observed_orcids":["https://orcid.org/0000-0003-0579-9897"]},"institutions":[{"id":"https://openalex.org/I1295876152","display_name":"Houston Methodist","ror":"https://ror.org/027zt9171","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1295876152"]},{"id":"https://openalex.org/I19820366","display_name":"Chinese Academy of Sciences","ror":"https://ror.org/034t30j35","country_code":"CN","type":"government","lineage":["https://openalex.org/I19820366"]},{"id":"https://openalex.org/I4210141483","display_name":"National Center for Nanoscience and Technology","ror":"https://ror.org/04f49ff35","country_code":"CN","type":"nonprofit","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210141483"]},{"id":"https://openalex.org/I4405286254","display_name":"Houston Methodist Research Institute","ror":"https://ror.org/01mcrxx35","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I1295876152","https://openalex.org/I4405286254"]}],"countries":["CN","US"],"is_corresponding":false,"raw_author_name":"Joy Wolfram","raw_affiliation_strings":["CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, National Center for Nanoscience & Technology of China","Department of Nanomedicine, Houston Methodist Research Institute"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, National Center for Nanoscience & Technology of China","institution_ids":["https://openalex.org/I19820366","https://openalex.org/I4210141483"]},{"raw_affiliation_string":"Department of Nanomedicine, Houston Methodist Research Institute","institution_ids":["https://openalex.org/I1295876152","https://openalex.org/I4405286254"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5060167040","display_name":"Zhizhou Yang","orcid":"https://orcid.org/0000-0002-0921-3606","observed_orcids":["https://orcid.org/0000-0002-0921-3606"]},"institutions":[{"id":"https://openalex.org/I1295876152","display_name":"Houston Methodist","ror":"https://ror.org/027zt9171","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1295876152"]},{"id":"https://openalex.org/I4405286254","display_name":"Houston Methodist Research Institute","ror":"https://ror.org/01mcrxx35","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I1295876152","https://openalex.org/I4405286254"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Zhizhou Yang","raw_affiliation_strings":["Department of Nanomedicine, Houston Methodist Research Institute"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Nanomedicine, Houston Methodist Research Institute","institution_ids":["https://openalex.org/I1295876152","https://openalex.org/I4405286254"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5046955595","display_name":"Zong\u2010Wan Mao","orcid":"https://orcid.org/0000-0001-7131-1154","observed_orcids":["https://orcid.org/0000-0001-7131-1154"]},"institutions":[{"id":"https://openalex.org/I157773358","display_name":"Sun Yat-sen University","ror":"https://ror.org/0064kty71","country_code":"CN","type":"education","lineage":["https://openalex.org/I157773358"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Zong-Wan Mao","raw_affiliation_strings":["MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-sen University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-sen University","institution_ids":["https://openalex.org/I157773358"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5070051249","display_name":"Mauro Ferrari B","orcid":"https://orcid.org/0000-0001-7042-0236","observed_orcids":["https://orcid.org/0000-0001-7042-0236"]},"institutions":[{"id":"https://openalex.org/I1295876152","display_name":"Houston Methodist","ror":"https://ror.org/027zt9171","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1295876152"]},{"id":"https://openalex.org/I4387153466","display_name":"Weill Cornell Medicine","ror":"https://ror.org/02r109517","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295","https://openalex.org/I4387153466"]},{"id":"https://openalex.org/I4405286254","display_name":"Houston Methodist Research Institute","ror":"https://ror.org/01mcrxx35","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I1295876152","https://openalex.org/I4405286254"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Mauro Ferrari","raw_affiliation_strings":["Department of Medicine, Weill Cornell Medical College","Department of Nanomedicine, Houston Methodist Research Institute"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, Weill Cornell Medical College","institution_ids":["https://openalex.org/I4387153466"]},{"raw_affiliation_string":"Department of Nanomedicine, Houston Methodist Research Institute","institution_ids":["https://openalex.org/I1295876152","https://openalex.org/I4405286254"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5086981177","display_name":"Haifa Shen","orcid":"https://orcid.org/0000-0002-9728-5430","observed_orcids":["https://orcid.org/0000-0002-9728-5430"]},"institutions":[{"id":"https://openalex.org/I1295876152","display_name":"Houston Methodist","ror":"https://ror.org/027zt9171","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1295876152"]},{"id":"https://openalex.org/I4387153466","display_name":"Weill Cornell Medicine","ror":"https://ror.org/02r109517","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295","https://openalex.org/I4387153466"]},{"id":"https://openalex.org/I4405286254","display_name":"Houston Methodist Research Institute","ror":"https://ror.org/01mcrxx35","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I1295876152","https://openalex.org/I4405286254"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Haifa Shen","raw_affiliation_strings":["Department of Cell and Developmental Biology, Weill Cornell Medical College","Department of Nanomedicine, Houston Methodist Research Institute"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell and Developmental Biology, Weill Cornell Medical College","institution_ids":["https://openalex.org/I4387153466"]},{"raw_affiliation_string":"Department of Nanomedicine, Houston Methodist Research Institute","institution_ids":["https://openalex.org/I1295876152","https://openalex.org/I4405286254"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":7,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.8675,"has_fulltext":false,"cited_by_count":12,"citation_normalized_percentile":{"value":0.73506684,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":null,"issue":"95","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10063","display_name":"Nanoparticle-Based Drug Delivery","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/2502","display_name":"Biomaterials"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11169","display_name":"Silicon Nanostructures and Photoluminescence","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/sirna-delivery","display_name":"siRNA delivery","score":0.925000011920929},{"id":"https://openalex.org/keywords/polyethylenimine","display_name":"polyethyleneimine","score":0.9559999704360962},{"id":"https://openalex.org/keywords/gene-silencing","display_name":"gene silencing","score":0.6850000023841858},{"id":"https://openalex.org/keywords/sirna-nanoparticles","display_name":"siRNA nanoparticles","score":0.47200000286102295},{"id":"https://openalex.org/keywords/particle-degradation","display_name":"particle degradation","score":0.4830000102519989},{"id":"https://openalex.org/keywords/cytotoxicity","display_name":"cytotoxicity","score":0.9200000166893005},{"id":"https://openalex.org/keywords/cellular-uptake","display_name":"cellular uptake","score":0.6620000004768372}],"concepts":[{"id":"https://openalex.org/C2775925850","wikidata":"https://www.wikidata.org/wiki/Q418697","display_name":"Polyethylenimine","level":4,"score":0.8575738668441772},{"id":"https://openalex.org/C22615655","wikidata":"https://www.wikidata.org/wiki/Q203221","display_name":"Small interfering RNA","level":4,"score":0.7923809289932251},{"id":"https://openalex.org/C109316439","wikidata":"https://www.wikidata.org/wiki/Q246181","display_name":"Cytotoxicity","level":3,"score":0.721693754196167},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.6223046779632568},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.4999516010284424},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.46262669563293457},{"id":"https://openalex.org/C2776685891","wikidata":"https://www.wikidata.org/wiki/Q428660","display_name":"Porous silicon","level":3,"score":0.46041399240493774},{"id":"https://openalex.org/C135983454","wikidata":"https://www.wikidata.org/wiki/Q2790163","display_name":"Gene delivery","level":4,"score":0.44521763920783997},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.441322386264801},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.42454200983047485},{"id":"https://openalex.org/C1318750","wikidata":"https://www.wikidata.org/wiki/Q865663","display_name":"Biocompatible material","level":2,"score":0.4227173328399658},{"id":"https://openalex.org/C115537861","wikidata":"https://www.wikidata.org/wiki/Q7645993","display_name":"Surface modification","level":2,"score":0.4191280007362366},{"id":"https://openalex.org/C79879829","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Intracellular","level":2,"score":0.4110986590385437},{"id":"https://openalex.org/C136229726","wikidata":"https://www.wikidata.org/wiki/Q327092","display_name":"Biomedical engineering","level":1,"score":0.25211548805236816},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.20959067344665527},{"id":"https://openalex.org/C544956773","wikidata":"https://www.wikidata.org/wiki/Q670","display_name":"Silicon","level":2,"score":0.18584054708480835},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.14409378170967102},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.1383296549320221},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.10538321733474731},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.07557839155197144},{"id":"https://openalex.org/C147789679","wikidata":"https://www.wikidata.org/wiki/Q11372","display_name":"Physical chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.3791/52075-v","doi":"https://doi.org/10.3791/52075-v","is_oa":false,"landing_page_url":"https://doi.org/10.3791/52075-v","pdf_url":null,"source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.5683000087738037,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1801554552","https://openalex.org/W4206971604","https://openalex.org/W2049648258","https://openalex.org/W4410251099","https://openalex.org/W2122777061","https://openalex.org/W2325021555","https://openalex.org/W2085287701","https://openalex.org/W2024966238","https://openalex.org/W1992850149","https://openalex.org/W2116712417"],"abstract_inverted_index":{"Small":[0],"interfering":[1],"RNA":[2],"(siRNA)":[3],"can":[4,36,85],"be":[5,86],"used":[6],"to":[7],"suppress":[8],"gene":[9],"expression,":[10],"thereby":[11,104],"providing":[12],"a":[13,55,63,92,115],"new":[14],"avenue":[15],"for":[16,57,117,144],"the":[17,23,30,38,58,106,142],"treatment":[18],"of":[19,26,32,41,62,75,108,128],"various":[20],"diseases.":[21],"However,":[22],"successful":[24],"implementation":[25],"siRNA":[27,42,67,89,134],"therapy":[28],"requires":[29],"use":[31,61],"delivery":[33,68,112],"platforms":[34],"that":[35],"overcome":[37],"major":[39,126],"challenges":[40],"delivery,":[43],"such":[44],"as":[45],"enzymatic":[46],"degradation,":[47],"low":[48],"intracellular":[49],"uptake":[50],"and":[51,60,65,78,119,133,149],"lysosomal":[52],"entrapment.":[53],"Here,":[54],"protocol":[56],"preparation":[59],"biocompatible":[64],"effective":[66],"system":[69],"is":[70],"presented.":[71],"This":[72,111],"platform":[73],"consists":[74],"polyethylenimine":[76],"(PEI)":[77],"arginine":[79],"(Arg)-grafted":[80],"porous":[81],"silicon":[82,97],"microparticles,":[83],"which":[84],"loaded":[87],"with":[88],"by":[90],"performing":[91],"simple":[93],"mixing":[94],"step.":[95],"The":[96,125],"particles":[98],"are":[99,136,152],"gradually":[100],"degraded":[101],"over":[102],"time,":[103],"triggering":[105],"formation":[107],"Arg-PEI/siRNA":[109],"nanoparticles.":[110],"vehicle":[113],"provides":[114],"means":[116],"protecting":[118],"internalizing":[120],"siRNA,":[121],"without":[122],"causing":[123],"cytotoxicity.":[124],"steps":[127],"polycation":[129],"functionalization,":[130],"particle":[131,146],"characterization,":[132],"loading":[135],"outlined":[137],"in":[138],"detail.":[139],"In":[140],"addition,":[141],"procedures":[143],"determining":[145],"uptake,":[147],"cytotoxicity,":[148],"transfection":[150],"efficacy":[151],"also":[153],"described.":[154]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":2}],"updated_date":"2026-10-24T09:50:30.924529","created_date":"2022-07-25T00:00:00"}