{"id":"https://openalex.org/W4286852969","doi":"https://doi.org/10.3791/54219-v","title":"Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions","display_name":"Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions","publication_year":2016,"publication_date":"2016-07-30","ids":{"openalex":"https://openalex.org/W4286852969","doi":"https://doi.org/10.3791/54219-v"},"language":"en","primary_location":{"id":"doi:10.3791/54219-v","doi":"https://doi.org/10.3791/54219-v","is_oa":false,"landing_page_url":"https://doi.org/10.3791/54219-v","pdf_url":null,"source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5051929541","display_name":"Melinda L. Koyle","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I100005738","display_name":"Brigham Young University","ror":"https://ror.org/047rhhm47","country_code":"US","type":"education","lineage":["https://openalex.org/I100005738"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Melinda L. Koyle","raw_affiliation_strings":["Department of Plant and Wildlife Sciences, Brigham Young University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Plant and Wildlife Sciences, Brigham Young University","institution_ids":["https://openalex.org/I100005738"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5033711118","display_name":"Madeline Veloz","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I100005738","display_name":"Brigham Young University","ror":"https://ror.org/047rhhm47","country_code":"US","type":"education","lineage":["https://openalex.org/I100005738"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Madeline Veloz","raw_affiliation_strings":["Department of Plant and Wildlife Sciences, Brigham Young University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Plant and Wildlife Sciences, Brigham Young University","institution_ids":["https://openalex.org/I100005738"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5079271953","display_name":"Alec M. Judd","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I100005738","display_name":"Brigham Young University","ror":"https://ror.org/047rhhm47","country_code":"US","type":"education","lineage":["https://openalex.org/I100005738"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Alec M. Judd","raw_affiliation_strings":["Department of Plant and Wildlife Sciences, Brigham Young University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Plant and Wildlife Sciences, Brigham Young University","institution_ids":["https://openalex.org/I100005738"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5033907333","display_name":"Adam Chun-Nin Wong","orcid":"https://orcid.org/0000-0001-9360-429X","observed_orcids":["https://orcid.org/0000-0001-9360-429X"]},"institutions":[{"id":"https://openalex.org/I1288882113","display_name":"Boston Children's Hospital","ror":"https://ror.org/00dvg7y05","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1288882113"]},{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]},{"id":"https://openalex.org/I205783295","display_name":"Cornell University","ror":"https://ror.org/05bnh6r87","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Adam C.-N. Wong","raw_affiliation_strings":["Department of Entomology, Cornell University","Division of Infectious Diseases, Boston Children's Hospital, Harvard Medical School"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Entomology, Cornell University","institution_ids":["https://openalex.org/I205783295"]},{"raw_affiliation_string":"Division of Infectious Diseases, Boston Children's Hospital, Harvard Medical School","institution_ids":["https://openalex.org/I1288882113","https://openalex.org/I136199984"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5071652506","display_name":"Peter D. Newell","orcid":"https://orcid.org/0000-0002-0959-5907","observed_orcids":["https://orcid.org/0000-0002-0959-5907"]},"institutions":[{"id":"https://openalex.org/I205783295","display_name":"Cornell University","ror":"https://ror.org/05bnh6r87","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295"]},{"id":"https://openalex.org/I43742981","display_name":"State University of New York at Oswego","ror":"https://ror.org/01597g643","country_code":"US","type":"education","lineage":["https://openalex.org/I43742981"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Peter D. Newell","raw_affiliation_strings":["Biological Sciences, SUNY Oswego","Department of Entomology, Cornell University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biological Sciences, SUNY Oswego","institution_ids":["https://openalex.org/I43742981"]},{"raw_affiliation_string":"Department of Entomology, Cornell University","institution_ids":["https://openalex.org/I205783295"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5050851681","display_name":"Angela E. Douglas","orcid":"https://orcid.org/0000-0001-5212-6826","observed_orcids":["https://orcid.org/0000-0001-5212-6826"]},"institutions":[{"id":"https://openalex.org/I205783295","display_name":"Cornell University","ror":"https://ror.org/05bnh6r87","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Angela E. Douglas","raw_affiliation_strings":["Department of Entomology, Cornell University","Department of Molecular Biology and Genetics, Cornell University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Entomology, Cornell University","institution_ids":["https://openalex.org/I205783295"]},{"raw_affiliation_string":"Department of Molecular Biology and Genetics, Cornell University","institution_ids":["https://openalex.org/I205783295"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5070939603","display_name":"John M. Chaston","orcid":"https://orcid.org/0000-0002-2625-6413","observed_orcids":["https://orcid.org/0000-0002-2625-6413"]},"institutions":[{"id":"https://openalex.org/I100005738","display_name":"Brigham Young University","ror":"https://ror.org/047rhhm47","country_code":"US","type":"education","lineage":["https://openalex.org/I100005738"]},{"id":"https://openalex.org/I205783295","display_name":"Cornell University","ror":"https://ror.org/05bnh6r87","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"John M. Chaston","raw_affiliation_strings":["Department of Entomology, Cornell University","Department of Plant and Wildlife Sciences, Brigham Young University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Entomology, Cornell University","institution_ids":["https://openalex.org/I205783295"]},{"raw_affiliation_string":"Department of Plant and Wildlife Sciences, Brigham Young University","institution_ids":["https://openalex.org/I100005738"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":5,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.7238,"has_fulltext":false,"cited_by_count":27,"citation_normalized_percentile":{"value":0.77543744,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":99},"biblio":{"volume":null,"issue":"113","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12134","display_name":"Insect symbiosis and bacterial influences","score":0.7339000105857849,"subfield":{"id":"https://openalex.org/subfields/1109","display_name":"Insect Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12134","display_name":"Insect symbiosis and bacterial influences","score":0.7339000105857849,"subfield":{"id":"https://openalex.org/subfields/1109","display_name":"Insect Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10066","display_name":"Gut microbiota and health","score":0.09160000085830688,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10423","display_name":"Neurobiology and Insect Physiology Research","score":0.0729999989271164,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/drosophila-melanogaster","display_name":"Drosophila melanogaster","score":0.9990000128746033},{"id":"https://openalex.org/keywords/axenic-rearing","display_name":"axenic rearing","score":0.9369999766349792},{"id":"https://openalex.org/keywords/microbial-interactions","display_name":"microbial interactions","score":0.36000001430511475},{"id":"https://openalex.org/keywords/bacterial-inoculation","display_name":"bacterial inoculation","score":0.8730000257492065},{"id":"https://openalex.org/keywords/bacterial-community-composition","display_name":"bacterial community composition","score":0.7850000262260437},{"id":"https://openalex.org/keywords/microbiota-transfer","display_name":"microbiota transfer","score":0.375}],"concepts":[{"id":"https://openalex.org/C2778265057","wikidata":"https://www.wikidata.org/wiki/Q537299","display_name":"Axenic","level":3,"score":0.9528090953826904},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8662149906158447},{"id":"https://openalex.org/C2780104201","wikidata":"https://www.wikidata.org/wiki/Q130888","display_name":"Drosophila melanogaster","level":3,"score":0.7159913778305054},{"id":"https://openalex.org/C2776041557","wikidata":"https://www.wikidata.org/wiki/Q3715451","display_name":"Drosophila (subgenus)","level":3,"score":0.7066603899002075},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.5235645174980164},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.4521484971046448},{"id":"https://openalex.org/C148761745","wikidata":"https://www.wikidata.org/wiki/Q1563203","display_name":"Sterilization (economics)","level":4,"score":0.4238434135913849},{"id":"https://openalex.org/C168456226","wikidata":"https://www.wikidata.org/wiki/Q1462045","display_name":"Sterile insect technique","level":3,"score":0.41865837574005127},{"id":"https://openalex.org/C90856448","wikidata":"https://www.wikidata.org/wiki/Q431","display_name":"Zoology","level":1,"score":0.34968236088752747},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.2259749174118042},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.18678635358810425},{"id":"https://openalex.org/C22508944","wikidata":"https://www.wikidata.org/wiki/Q568174","display_name":"PEST analysis","level":2,"score":0.07844069600105286},{"id":"https://openalex.org/C556758197","wikidata":"https://www.wikidata.org/wiki/Q580018","display_name":"Monetary economics","level":1,"score":0.0},{"id":"https://openalex.org/C2983132609","wikidata":"https://www.wikidata.org/wiki/Q66076","display_name":"Foreign exchange","level":2,"score":0.0},{"id":"https://openalex.org/C162324750","wikidata":"https://www.wikidata.org/wiki/Q8134","display_name":"Economics","level":0,"score":0.0},{"id":"https://openalex.org/C536366893","wikidata":"https://www.wikidata.org/wiki/Q66076","display_name":"Foreign exchange market","level":3,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.3791/54219-v","doi":"https://doi.org/10.3791/54219-v","is_oa":false,"landing_page_url":"https://doi.org/10.3791/54219-v","pdf_url":null,"source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2808944050","https://openalex.org/W1847696276","https://openalex.org/W7071829566","https://openalex.org/W6983384415","https://openalex.org/W3093111223","https://openalex.org/W2219986505","https://openalex.org/W7095423181","https://openalex.org/W2166674630","https://openalex.org/W2097518606","https://openalex.org/W2756165608"],"abstract_inverted_index":{"The":[0,17],"influence":[1],"of":[2,40,59,90,117,123],"microbes":[3,93],"on":[4],"myriad":[5],"animal":[6,30],"traits":[7],"and":[8,64,66,83,86,112],"behaviors":[9],"has":[10],"been":[11],"increasingly":[12],"recognized":[13],"in":[14,78,133],"recent":[15],"years.":[16],"fruit":[18],"fly":[19],"Drosophila":[20,41,60,148],"melanogaster":[21],"is":[22],"a":[23,55,102,129],"model":[24],"for":[25,57,139],"understanding":[26],"microbial":[27,49,115],"interactions":[28],"with":[29,47,101,114],"hosts,":[31],"facilitated":[32],"by":[33,108],"approaches":[34,138],"to":[35,68,75,127],"rear":[36],"large":[37],"sample":[38],"sizes":[39],"under":[42],"microorganism-free":[43],"(axenic)":[44],"conditions,":[45],"or":[46],"defined":[48,103],"communities":[50],"(gnotobiotic).":[51],"This":[52],"work":[53],"outlines":[54],"method":[56],"collection":[58],"embryos,":[61],"hypochlorite":[62],"dechorionation":[63],"sterilization,":[65],"transfer":[67],"sterile":[69,76,110],"diet.":[70],"Sterilized":[71],"embryos":[72,113],"are":[73,97],"transferred":[74],"diet":[77,111],"50":[79],"ml":[80],"centrifuge":[81],"tubes,":[82],"developing":[84],"larvae":[85],"adults":[87],"remain":[88,149],"free":[89],"any":[91],"exogenous":[92],"until":[94],"the":[95,121],"vials":[96],"opened.":[98],"Alternatively,":[99],"flies":[100],"microbiota":[104,132],"can":[105],"be":[106],"reared":[107],"inoculating":[109],"species":[116,126],"interest.":[118],"We":[119],"describe":[120,137],"introduction":[122],"4":[124],"bacterial":[125,141],"establish":[128],"representative":[130],"gnotobiotic":[131],"Drosophila.":[134],"Finally,":[135],"we":[136],"confirming":[140],"community":[142],"composition,":[143],"including":[144],"testing":[145],"if":[146],"axenic":[147],"bacteria-free":[150],"into":[151],"adulthood.":[152]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":4},{"year":2023,"cited_by_count":9},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":7},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":1}],"updated_date":"2026-10-08T09:06:37.294493","created_date":"2022-07-25T00:00:00"}
