{"id":"https://openalex.org/W4286852848","doi":"https://doi.org/10.3791/3886-v","title":"Micropipette Aspiration of Substrate-attached Cells to Estimate Cell Stiffness","display_name":"Micropipette Aspiration of Substrate-attached Cells to Estimate Cell Stiffness","publication_year":2012,"publication_date":"2012-09-27","ids":{"openalex":"https://openalex.org/W4286852848","doi":"https://doi.org/10.3791/3886-v"},"language":"en","primary_location":{"id":"doi:10.3791/3886-v","doi":"https://doi.org/10.3791/3886-v","is_oa":false,"landing_page_url":"https://doi.org/10.3791/3886-v","pdf_url":null,"source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5054572408","display_name":"Myungjin Oh","orcid":"https://orcid.org/0000-0002-2604-1818","observed_orcids":["https://orcid.org/0000-0002-2604-1818"]},"institutions":[{"id":"https://openalex.org/I157725225","display_name":"University of Illinois Urbana-Champaign","ror":"https://ror.org/047426m28","country_code":"US","type":"education","lineage":["https://openalex.org/I157725225"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Myung-Jin Oh","raw_affiliation_strings":["Section of Respiratory, Critical Care and Sleep Medicine, Department of Medicine, University of Illinois"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Section of Respiratory, Critical Care and Sleep Medicine, Department of Medicine, University of Illinois","institution_ids":["https://openalex.org/I157725225"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5035539196","display_name":"Frank Kuhr","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I157725225","display_name":"University of Illinois Urbana-Champaign","ror":"https://ror.org/047426m28","country_code":"US","type":"education","lineage":["https://openalex.org/I157725225"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Frank Kuhr","raw_affiliation_strings":["Section of Respiratory, Critical Care and Sleep Medicine, Department of Medicine, University of Illinois"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Section of Respiratory, Critical Care and Sleep Medicine, Department of Medicine, University of Illinois","institution_ids":["https://openalex.org/I157725225"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5079526343","display_name":"Fitzroy J. Byfield","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I79576946","display_name":"University of Pennsylvania","ror":"https://ror.org/00b30xv10","country_code":"US","type":"education","lineage":["https://openalex.org/I79576946"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Fitzroy Byfield","raw_affiliation_strings":["Institute for Medicine and Engineering, University of Pennsylvania"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Medicine and Engineering, University of Pennsylvania","institution_ids":["https://openalex.org/I79576946"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5086847276","display_name":"Irena Levitan","orcid":"https://orcid.org/0000-0003-3072-6497","observed_orcids":["https://orcid.org/0000-0003-3072-6497"]},"institutions":[{"id":"https://openalex.org/I157725225","display_name":"University of Illinois Urbana-Champaign","ror":"https://ror.org/047426m28","country_code":"US","type":"education","lineage":["https://openalex.org/I157725225"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Irena Levitan","raw_affiliation_strings":["Section of Respiratory, Critical Care and Sleep Medicine, Department of Medicine, University of Illinois"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Section of Respiratory, Critical Care and Sleep Medicine, Department of Medicine, University of Illinois","institution_ids":["https://openalex.org/I157725225"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":6,"citation_normalized_percentile":{"value":0.3877335,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":95},"biblio":{"volume":null,"issue":"67","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10379","display_name":"Cellular Mechanics and Interactions","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10379","display_name":"Cellular Mechanics and Interactions","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10923","display_name":"Force Microscopy Techniques and Applications","score":0.00039999998989515007,"subfield":{"id":"https://openalex.org/subfields/3107","display_name":"Atomic and Molecular Physics, and Optics"},"field":{"id":"https://openalex.org/fields/31","display_name":"Physics and Astronomy"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12859","display_name":"Cell Image Analysis Techniques","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cell-stiffness","display_name":"cell stiffness","score":0.8960000276565552},{"id":"https://openalex.org/keywords/micropipette-aspiration","display_name":"micropipette aspiration","score":0.9449999928474426},{"id":"https://openalex.org/keywords/membrane-deformation","display_name":"membrane deformation","score":0.7580000162124634},{"id":"https://openalex.org/keywords/cell-mechanics","display_name":"cell mechanics","score":0.7789999842643738},{"id":"https://openalex.org/keywords/young-s-modulus","display_name":"Young's modulus","score":0.6880000233650208},{"id":"https://openalex.org/keywords/membrane-stiffness","display_name":"membrane rigidity","score":0.5529999732971191},{"id":"https://openalex.org/keywords/cell-deformability","display_name":"cell deformability","score":0.5929999947547913},{"id":"https://openalex.org/keywords/liposomes","display_name":"liposomes","score":0.4320000112056732},{"id":"https://openalex.org/keywords/organelles","display_name":"organelles","score":0.7889999747276306}],"concepts":[{"id":"https://openalex.org/C151552171","wikidata":"https://www.wikidata.org/wiki/Q163373","display_name":"Pipette","level":2,"score":0.9817383885383606},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.671211302280426},{"id":"https://openalex.org/C2779372316","wikidata":"https://www.wikidata.org/wiki/Q569057","display_name":"Stiffness","level":2,"score":0.617777943611145},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.5231077671051025},{"id":"https://openalex.org/C56318395","wikidata":"https://www.wikidata.org/wiki/Q215928","display_name":"Transducer","level":2,"score":0.5131369233131409},{"id":"https://openalex.org/C136229726","wikidata":"https://www.wikidata.org/wiki/Q327092","display_name":"Biomedical engineering","level":1,"score":0.5019142627716064},{"id":"https://openalex.org/C2778106830","wikidata":"https://www.wikidata.org/wiki/Q29548","display_name":"Cell membrane","level":3,"score":0.4679483473300934},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.4621155560016632},{"id":"https://openalex.org/C204366326","wikidata":"https://www.wikidata.org/wiki/Q3027650","display_name":"Deformation (meteorology)","level":2,"score":0.4301709830760956},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.34553995728492737},{"id":"https://openalex.org/C159985019","wikidata":"https://www.wikidata.org/wiki/Q181790","display_name":"Composite material","level":1,"score":0.19779303669929504},{"id":"https://openalex.org/C24890656","wikidata":"https://www.wikidata.org/wiki/Q82811","display_name":"Acoustics","level":1,"score":0.12050598859786987},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.1072239875793457},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.10519605875015259},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.09826558828353882},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.06834283471107483},{"id":"https://openalex.org/C147789679","wikidata":"https://www.wikidata.org/wiki/Q11372","display_name":"Physical chemistry","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.3791/3886-v","doi":"https://doi.org/10.3791/3886-v","is_oa":false,"landing_page_url":"https://doi.org/10.3791/3886-v","pdf_url":null,"source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1981146550","https://openalex.org/W2909930030","https://openalex.org/W2134763908","https://openalex.org/W2581739871","https://openalex.org/W2726812785","https://openalex.org/W2360327121","https://openalex.org/W2531752572","https://openalex.org/W4242775240","https://openalex.org/W2166926030","https://openalex.org/W2043957547"],"abstract_inverted_index":{"Growing":[0],"number":[1],"of":[2,8,30,41,65,115,137,146,154,200,205,213,226,238,294,300,316,327,337,368,375,387],"studies":[3],"show":[4],"that":[5,84,210,334,358,363],"biomechanical":[6,385],"properties":[7,386],"individual":[9],"cells":[10,43,318,339],"play":[11],"major":[12],"roles":[13],"in":[14,68,216,256],"multiple":[15],"cellular":[16,31,34,377],"functions,":[17],"including":[18],"cell":[19,59,80,117,139,172,279],"proliferation,":[20],"differentiation,":[21],"migration":[22],"and":[23,38,46,54,131,185,241,258,262,357,371],"cell-cell":[24],"interactions.":[25],"The":[26,202,229],"two":[27],"key":[28],"parameters":[29],"biomechanics":[32],"are":[33],"deformability":[35,374],"or":[36,89,118,189,281],"stiffness":[37,60,326],"the":[39,42,63,79,113,144,147,160,163,171,178,183,193,211,231,234,236,242,244,291,301,305,313,317,320,354,361,365,369,373,376,384,388],"ability":[40],"to":[44,57,70,78,99,126,133,159,176,218,311,324,332],"contract":[45],"generate":[47],"force.":[48],"Here":[49],"we":[50],"describe":[51],"a":[52,75,82,96,101,104,116,119,127,134,138,141,150,152,198,219,224,274,298,344],"quick":[53],"simple":[55],"method":[56],"estimate":[58,312],"by":[61,74,94,162,267,289,353],"measuring":[62],"degree":[64,212],"membrane":[66,179,187,214,227,232,239,270,295,348,370],"deformation":[67,188,215,240,296],"response":[69,175,217],"negative":[71,155],"pressure":[72,129,156,165,169,276],"applied":[73,158,302],"glass":[76,97],"micropipette":[77,102],"surface,":[81],"technique":[83,248],"is":[85,92,123,157,180,195,209,223,265,287,330,351,360],"called":[86],"Micropipette":[87],"Aspiration":[88],"Microaspiration.":[90],"Microaspiration":[91],"performed":[93,251,266],"pulling":[95],"capillary":[98],"create":[100],"with":[103],"very":[105],"small":[106],"tip":[107,145],"(2-50":[108],"\u03bcm":[109],"diameter":[110],"depending":[111],"on":[112,170,252],"size":[114],"tissue":[120],"sample),":[121],"which":[122],"then":[124],"connected":[125],"pneumatic":[128,164],"transducer":[130,166],"brought":[132],"close":[135],"vicinity":[136],"under":[140,273],"microscope.":[142],"When":[143],"pipette":[148,161,184,194],"touches":[149],"cell,":[151],"step":[153],"generating":[167],"well-defined":[168],"membrane.":[173],"In":[174],"pressure,":[177],"aspirated":[181,292],"into":[182,192],"progressive":[186],"\"membrane":[190],"projection\"":[191],"measured":[196],"as":[197,297,343],"function":[199,225,299],"time.":[201],"basic":[203],"principle":[204],"this":[206],"experimental":[207,282],"approach":[208],"defined":[220],"mechanical":[221,366],"force":[222],"stiffness.":[228],"stiffer":[230],"is,":[233],"slower":[235],"rate":[237],"shorter":[243],"steady-state":[245],"aspiration":[246],"length.The":[247],"can":[249,307,340],"be":[250,308,341],"isolated":[253],"cells,":[254],"both":[255],"suspension":[257],"substrate-attached,":[259],"large":[260],"organelles,":[261],"liposomes.":[263],"Analysis":[264],"comparing":[268],"maximal":[269],"deformations":[271],"achieved":[272],"given":[275],"for":[277],"different":[278],"populations":[280],"conditions.":[283],"A":[284],"\"stiffness":[285],"coefficient\"":[286],"estimated":[288],"plotting":[290],"length":[293],"pressure.":[303],"Furthermore,":[304],"data":[306],"further":[309],"analyzed":[310],"Young's":[314],"modulus":[315],"(E),":[319],"most":[321],"common":[322],"parameter":[323],"characterize":[325],"materials.":[328],"It":[329],"important":[331],"note":[333],"plasma":[335],"membranes":[336],"eukaryotic":[338],"viewed":[342],"bi-component":[345],"system":[346],"where":[347],"lipid":[349],"bilayer":[350],"underlied":[352],"sub-membrane":[355,389],"cytoskeleton":[356,362],"it":[359],"constitutes":[364],"scaffold":[367],"dominates":[372],"envelope.":[378],"This":[379],"approach,":[380],"therefore,":[381],"allows":[382],"probing":[383],"cytoskeleton.":[390]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":1},{"year":2017,"cited_by_count":1}],"updated_date":"2026-11-03T10:50:19.383937","created_date":"2022-07-25T00:00:00"}
