{"id":"https://openalex.org/W4286852472","doi":"https://doi.org/10.3791/55835-v","title":"Enrichment of Detergent-insoluble Protein Aggregates from Human Postmortem Brain","display_name":"Enrichment of Detergent-insoluble Protein Aggregates from Human Postmortem Brain","publication_year":2017,"publication_date":"2017-10-24","ids":{"openalex":"https://openalex.org/W4286852472","doi":"https://doi.org/10.3791/55835-v"},"language":"en","primary_location":{"id":"doi:10.3791/55835-v","doi":"https://doi.org/10.3791/55835-v","is_oa":false,"landing_page_url":"https://doi.org/10.3791/55835-v","pdf_url":null,"source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5061860283","display_name":"Ian Diner","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I150468666","display_name":"Emory University","ror":"https://ror.org/03czfpz43","country_code":"US","type":"education","lineage":["https://openalex.org/I150468666"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ian Diner","raw_affiliation_strings":["Department of Biochemistry, Emory School of Medicine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Emory School of Medicine","institution_ids":["https://openalex.org/I150468666"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5091811269","display_name":"Tram Nguyen","orcid":"https://orcid.org/0000-0002-5499-2397","observed_orcids":["https://orcid.org/0000-0002-5499-2397"]},"institutions":[{"id":"https://openalex.org/I150468666","display_name":"Emory University","ror":"https://ror.org/03czfpz43","country_code":"US","type":"education","lineage":["https://openalex.org/I150468666"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Tram Nguyen","raw_affiliation_strings":["Department of Biochemistry, Emory School of Medicine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Emory School of Medicine","institution_ids":["https://openalex.org/I150468666"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5022349887","display_name":"Nicholas T. Seyfried","orcid":"https://orcid.org/0000-0002-4507-624X","observed_orcids":["https://orcid.org/0000-0002-4507-624X"]},"institutions":[{"id":"https://openalex.org/I150468666","display_name":"Emory University","ror":"https://ror.org/03czfpz43","country_code":"US","type":"education","lineage":["https://openalex.org/I150468666"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Nicholas T. Seyfried","raw_affiliation_strings":["Department of Biochemistry, Emory School of Medicine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Emory School of Medicine","institution_ids":["https://openalex.org/I150468666"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I150468666"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":10,"citation_normalized_percentile":{"value":0.42404833,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":90,"max":97},"biblio":{"volume":null,"issue":"128","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10086","display_name":"Alzheimer's disease research and treatments","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2737","display_name":"Physiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10086","display_name":"Alzheimer's disease research and treatments","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2737","display_name":"Physiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10009","display_name":"Dementia and Cognitive Impairment Research","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2738","display_name":"Psychiatry and Mental health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/postmortem-brain","display_name":"postmortem brain","score":0.8510000109672546},{"id":"https://openalex.org/keywords/alzheimer-s-disease","display_name":"Alzheimer's disease","score":0.9599999785423279},{"id":"https://openalex.org/keywords/neurodegenerative-proteinopathies","display_name":"neurodegenerative proteinopathies","score":0.8519999980926514},{"id":"https://openalex.org/keywords/phosphorylated-tau","display_name":"phosphorylated tau","score":0.9769999980926514},{"id":"https://openalex.org/keywords/neurofibrillary-tangles","display_name":"neurofibrillary tangles","score":0.968999981880188},{"id":"https://openalex.org/keywords/amyloid-beta","display_name":"amyloid-beta","score":0.8489999771118164},{"id":"https://openalex.org/keywords/eea1","display_name":"EEA1","score":0.7319999933242798},{"id":"https://openalex.org/keywords/apolipoprotein-e","display_name":"apolipoprotein E","score":0.9980000257492065}],"concepts":[{"id":"https://openalex.org/C46111723","wikidata":"https://www.wikidata.org/wiki/Q471857","display_name":"Proteomics","level":3,"score":0.577694296836853},{"id":"https://openalex.org/C136238340","wikidata":"https://www.wikidata.org/wiki/Q7251455","display_name":"Protein aggregation","level":2,"score":0.5048475861549377},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5034775137901306},{"id":"https://openalex.org/C85265743","wikidata":"https://www.wikidata.org/wiki/Q885458","display_name":"Blot","level":3,"score":0.49668532609939575},{"id":"https://openalex.org/C2777670902","wikidata":"https://www.wikidata.org/wiki/Q492038","display_name":"Human brain","level":2,"score":0.49316638708114624},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.47291240096092224},{"id":"https://openalex.org/C11960822","wikidata":"https://www.wikidata.org/wiki/Q242736","display_name":"Phosphorylation","level":2,"score":0.4299764931201935},{"id":"https://openalex.org/C27239569","wikidata":"https://www.wikidata.org/wiki/Q189029","display_name":"Cell fractionation","level":3,"score":0.4131809175014496},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.38849931955337524},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3331446647644043},{"id":"https://openalex.org/C169760540","wikidata":"https://www.wikidata.org/wiki/Q207011","display_name":"Neuroscience","level":1,"score":0.0},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.3791/55835-v","doi":"https://doi.org/10.3791/55835-v","is_oa":false,"landing_page_url":"https://doi.org/10.3791/55835-v","pdf_url":null,"source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.8371999859809875,"display_name":"Good health and well-being"}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4210389940","https://openalex.org/W4412795269","https://openalex.org/W4307091392","https://openalex.org/W4381849307","https://openalex.org/W4285180054","https://openalex.org/W2069570898","https://openalex.org/W2990899750","https://openalex.org/W2529144260","https://openalex.org/W2135857094","https://openalex.org/W2770086149"],"abstract_inverted_index":{"In":[0],"this":[1,154],"study,":[2],"we":[3],"describe":[4],"an":[5],"abbreviated":[6],"single-step":[7],"fractionation":[8,151],"protocol":[9,157],"for":[10,160],"the":[11,35,76,89,100,107,136],"enrichment":[12,36,124,156],"of":[13,37,45,78,92,103,125,139,145,164],"detergent-insoluble":[14,38,82],"protein":[15,39,83,173],"aggregates":[16,40,84],"from":[17,41,168],"human":[18],"postmortem":[19,66],"brain.":[20,119],"The":[21],"ionic":[22],"detergent":[23],"N-lauryl-sarcosine":[24],"(sarkosyl)":[25],"effectively":[26],"solubilizes":[27],"natively":[28],"folded":[29],"proteins":[30],"in":[31,75,95,115,135],"brain":[32,67,141],"tissue":[33,150],"allowing":[34],"a":[42,161],"wide":[43,162],"range":[44,163],"neurodegenerative":[46],"proteinopathies,":[47],"such":[48],"as":[49],"Alzheimer's":[50],"disease":[51,54],"(AD),":[52],"Parkinson's":[53],"and":[55,59,64,71,106,117,131,171],"amyotrophic":[56],"lateral":[57],"sclerosis,":[58],"prion":[60],"diseases.":[61],"Human":[62],"control":[63,116],"AD":[65,118,140],"tissues":[68],"were":[69],"homogenized":[70],"sedimented":[72],"by":[73],"ultracentrifugation":[74],"presence":[77],"sarkosyl":[79],"to":[80,143,176],"enrich":[81],"including":[85],"pathologic":[86],"phosphorylated":[87],"tau,":[88,128],"core":[90],"component":[91],"neurofibrillary":[93],"tangles":[94],"AD.":[96],"Western":[97,169],"blotting":[98,170],"demonstrated":[99],"differential":[101],"solubility":[102],"aggregated":[104],"phosphorylated-tau":[105],"detergent-soluble":[108],"protein,":[109],"Early":[110],"Endosome":[111],"Antigen":[112],"1":[113],"(EEA1)":[114],"Proteomic":[120],"analysis":[121],"also":[122],"revealed":[123],"\u03b2-amyloid":[126],"(A\u03b2),":[127],"snRNP70":[129],"(U1-70K),":[130],"apolipoprotein":[132],"E":[133],"(APOE)":[134],"sarkosyl-insoluble":[137],"fractions":[138],"compared":[142],"those":[144],"control,":[146],"consistent":[147],"with":[148],"previous":[149],"strategies.":[152],"Thus,":[153],"simple":[155],"is":[158],"ideal":[159],"experimental":[165],"applications":[166],"ranging":[167],"functional":[172],"co-aggregation":[174],"assays":[175],"mass":[177],"spectrometry-based":[178],"proteomics.":[179]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":3},{"year":2021,"cited_by_count":4}],"updated_date":"2026-10-08T09:06:37.294493","created_date":"2022-07-25T00:00:00"}
