{"id":"https://openalex.org/W4286851983","doi":"https://doi.org/10.3791/4141-v","title":"JoVE Video Dataset","display_name":"JoVE Video Dataset","publication_year":2021,"publication_date":"2021-11-19","ids":{"openalex":"https://openalex.org/W4286851983","doi":"https://doi.org/10.3791/4141-v"},"language":"pt","primary_location":{"id":"doi:10.3791/4141-v","doi":"https://doi.org/10.3791/4141-v","is_oa":false,"landing_page_url":"https://doi.org/10.3791/4141-v","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"database"},"volume":null,"type":"dataset","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5090812881","display_name":"Saijilafu","orcid":"https://orcid.org/0000-0003-2290-9808","observed_orcids":["https://orcid.org/0000-0003-2290-9808"]},"institutions":[{"id":"https://openalex.org/I145311948","display_name":"Johns Hopkins University","ror":"https://ror.org/00za53h95","country_code":"US","type":"education","lineage":["https://openalex.org/I145311948"]},{"id":"https://openalex.org/I2799853436","display_name":"Johns Hopkins Medicine","ror":"https://ror.org/037zgn354","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I2799853436"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Saijilafu","raw_affiliation_strings":["Department of Orthopaedic Surgery, Johns Hopkins University School of Medicine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Orthopaedic Surgery, Johns Hopkins University School of Medicine","institution_ids":["https://openalex.org/I145311948","https://openalex.org/I2799853436"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5054214253","display_name":"Feng\u2010Quan Zhou","orcid":"https://orcid.org/0000-0002-1525-9969","observed_orcids":["https://orcid.org/0000-0002-1525-9969"]},"institutions":[{"id":"https://openalex.org/I145311948","display_name":"Johns Hopkins University","ror":"https://ror.org/00za53h95","country_code":"US","type":"education","lineage":["https://openalex.org/I145311948"]},{"id":"https://openalex.org/I2799853436","display_name":"Johns Hopkins Medicine","ror":"https://ror.org/037zgn354","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I2799853436"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Feng-Quan Zhou","raw_affiliation_strings":["Department of Orthopaedic Surgery, Johns Hopkins University School of Medicine","Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Orthopaedic Surgery, Johns Hopkins University School of Medicine","institution_ids":["https://openalex.org/I145311948","https://openalex.org/I2799853436"]},{"raw_affiliation_string":"Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine","institution_ids":["https://openalex.org/I145311948","https://openalex.org/I2799853436"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10483","display_name":"Nerve injury and regeneration","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10483","display_name":"Nerve injury and regeneration","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10608","display_name":"Neurogenesis and neuroplasticity mechanisms","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/2806","display_name":"Developmental Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12061","display_name":"Axon Guidance and Neuronal Signaling","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dorsal-root-ganglion-neurons","display_name":"dorsal root ganglion neurons","score":0.9150000214576721},{"id":"https://openalex.org/keywords/axon-regeneration","display_name":"axon regeneration","score":0.9660000205039978},{"id":"https://openalex.org/keywords/c-jun","display_name":"c-Jun","score":0.9229999780654907},{"id":"https://openalex.org/keywords/axon-growth","display_name":"axon growth","score":0.8230000138282776},{"id":"https://openalex.org/keywords/central-nervous-system-injury","display_name":"central nervous system injury","score":0.597000002861023},{"id":"https://openalex.org/keywords/peripheral-nerve-injury","display_name":"peripheral nerve injury","score":0.5960000157356262},{"id":"https://openalex.org/keywords/cultured-neurons","display_name":"cultured neurons","score":0.492000013589859}],"concepts":[{"id":"https://openalex.org/C2779530196","wikidata":"https://www.wikidata.org/wiki/Q178999","display_name":"Axon","level":2,"score":0.8404017686843872},{"id":"https://openalex.org/C2776281502","wikidata":"https://www.wikidata.org/wiki/Q1395415","display_name":"Dorsal root ganglion","level":3,"score":0.7551737427711487},{"id":"https://openalex.org/C169760540","wikidata":"https://www.wikidata.org/wiki/Q207011","display_name":"Neuroscience","level":1,"score":0.7342337965965271},{"id":"https://openalex.org/C2780775167","wikidata":"https://www.wikidata.org/wiki/Q9606","display_name":"Spinal cord","level":2,"score":0.642802357673645},{"id":"https://openalex.org/C2779617337","wikidata":"https://www.wikidata.org/wiki/Q842429","display_name":"Soma","level":2,"score":0.6259642839431763},{"id":"https://openalex.org/C171056886","wikidata":"https://www.wikidata.org/wiki/Q193119","display_name":"Regeneration (biology)","level":2,"score":0.5784500241279602},{"id":"https://openalex.org/C529278444","wikidata":"https://www.wikidata.org/wiki/Q47273","display_name":"Central nervous system","level":2,"score":0.5249978303909302},{"id":"https://openalex.org/C545314008","wikidata":"https://www.wikidata.org/wiki/Q169953","display_name":"Peripheral nervous system","level":3,"score":0.5144057869911194},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5028983950614929},{"id":"https://openalex.org/C2778334475","wikidata":"https://www.wikidata.org/wiki/Q1415275","display_name":"Spinal cord injury","level":3,"score":0.5008640289306641},{"id":"https://openalex.org/C2778794669","wikidata":"https://www.wikidata.org/wiki/Q43054","display_name":"Neuron","level":2,"score":0.4496239125728607},{"id":"https://openalex.org/C2779683492","wikidata":"https://www.wikidata.org/wiki/Q792615","display_name":"Axotomy","level":3,"score":0.42818623781204224},{"id":"https://openalex.org/C105702510","wikidata":"https://www.wikidata.org/wiki/Q514","display_name":"Anatomy","level":1,"score":0.3885776996612549},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.1691388487815857}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.3791/4141-v","doi":"https://doi.org/10.3791/4141-v","is_oa":false,"landing_page_url":"https://doi.org/10.3791/4141-v","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"database"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.6240000128746033}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2065994644","https://openalex.org/W1984347000","https://openalex.org/W2889156800","https://openalex.org/W4413349063","https://openalex.org/W1988517763","https://openalex.org/W4386119311","https://openalex.org/W1546586156","https://openalex.org/W1996648228","https://openalex.org/W2072953174","https://openalex.org/W7171921084"],"abstract_inverted_index":{"It":[0],"is":[1,143,168,283],"well":[2,57],"known":[3,58],"that":[4,170,218],"mature":[5,34,38],"neurons":[6,39,55,152,180,194,235,245,274,328],"in":[7,32,40,104,111,162,228,266,289,329,344,357],"the":[8,33,41,73,94,99,112,118,122,129,136,147,154,163,171,261,279,301,310,330,333,349],"central":[9,90,109,141,148],"nervous":[10,43],"system":[11,44,202],"(CNS)":[12],"cannot":[13],"regenerate":[14,46,60,115],"their":[15],"axons":[16,102,110,142,149,161,324],"after":[17,48,62,117,286,320],"injuries":[18],"due":[19],"to":[20,24,59,128,184,203,259,325,347],"diminished":[21],"intrinsic":[22],"ability":[23],"support":[25],"axon":[26,71,106,175,187,206,226,267,295,353,359],"growth":[27,268,296,360],"and":[28,88,256,317],"a":[29,82,89,190,200,211,315],"hostile":[30,156],"environment":[31,157],"CNS1,2.":[35],"In":[36,230],"contrast,":[37],"peripheral":[42,63,83,86,101,123],"(PNS)":[45],"readily":[47],"injuries3.":[49],"Adult":[50],"dorsal":[51],"root":[52],"ganglion":[53],"(DRG)":[54],"are":[56,195,236,275],"robustly":[61],"nerve":[64],"injuries.":[65],"Each":[66],"DRG":[67,100,151,179,193,215,234,271,363],"neuron":[68,216],"grows":[69],"one":[70],"from":[72,269,327,361],"cell":[74],"soma,":[75],"which":[76,292,322],"branches":[77],"into":[78,93],"two":[79],"axonal":[80,124],"branches:":[81],"branch":[84,91],"innervating":[85],"targets":[87],"extending":[92],"spinal":[95,113,130,164],"cord.":[96,165],"Injury":[97],"of":[98,140,150,177,213,225,263,332,341,351],"results":[103],"substantial":[105],"regeneration,":[107],"whereas":[108],"cord":[114,131],"poorly":[116],"injury.":[119],"However,":[120],"if":[121],"injury":[125,132],"occurs":[126],"prior":[127],"(a":[133],"process":[134],"called":[135],"conditioning":[137],"lesion),":[138],"regeneration":[139,176,227],"greatly":[144],"improved4.":[145],"Moreover,":[146],"share":[153],"same":[155],"as":[158,199],"descending":[159],"corticospinal":[160],"Together,":[166],"it":[167],"hypothesized":[169],"molecular":[172],"mechanisms":[173],"controlling":[174],"adult":[178,192,214,233,270,362],"can":[181,219],"be":[182,220],"harnessed":[183],"enhance":[185],"CNS":[186],"regeneration.":[188],"As":[189],"result,":[191],"now":[196],"widely":[197],"used":[198,221],"model":[201,232,346],"study":[204,224,348],"regenerative":[205],"growth5-7.":[207],"Here":[208],"we":[209,337],"describe":[210],"method":[212,311],"culture":[217],"for":[222],"genetic":[223],"vitro.":[229],"this":[231,251,308,343],"genetically":[237],"manipulated":[238],"via":[239],"electroporation-mediated":[240],"gene":[241],"transfection6,8.":[242],"By":[243],"transfecting":[244],"with":[246,277],"DNA":[247],"plasmid":[248],"or":[249],"si/shRNA,":[250,278],"approach":[252],"enables":[253],"both":[254],"gain-":[255],"loss-of-function":[257,302],"experiments":[258],"investigate":[260],"role":[262,350],"any":[264],"gene-of-interest":[265],"neurons.":[272],"When":[273],"transfected":[276],"targeted":[280,334],"endogenous":[281],"protein":[282],"usually":[284],"depleted":[285],"3-4":[287],"days":[288],"culture,":[290],"during":[291],"time":[293],"robust":[294],"has":[297],"already":[298],"occurred,":[299],"making":[300],"studies":[303],"less":[304],"effective.":[305],"To":[306],"solve":[307],"problem,":[309],"described":[312],"here":[313],"includes":[314],"re-suspension":[316],"re-plating":[318],"step":[319],"transfection,":[321],"allows":[323],"re-grow":[326],"absence":[331],"protein.":[335],"Finally,":[336],"provide":[338],"an":[339,352],"example":[340],"using":[342],"vitro":[345],"regeneration-associated":[354],"gene,":[355],"c-Jun,":[356],"mediating":[358],"neurons9.":[364]},"counts_by_year":[],"updated_date":"2026-09-21T07:43:02.447757","created_date":"2022-07-25T00:00:00"}