{"id":"https://openalex.org/W4286850635","doi":"https://doi.org/10.3791/63264-v","title":"Visualizing and Quantifying Pharmaceutical Compounds within Skin using Coherent Raman Scattering Imaging","display_name":"Visualizing and Quantifying Pharmaceutical Compounds within Skin using Coherent Raman Scattering Imaging","publication_year":2021,"publication_date":"2021-11-24","ids":{"openalex":"https://openalex.org/W4286850635","doi":"https://doi.org/10.3791/63264-v"},"language":"en","primary_location":{"id":"doi:10.3791/63264-v","doi":"https://doi.org/10.3791/63264-v","is_oa":false,"landing_page_url":"https://doi.org/10.3791/63264-v","pdf_url":null,"source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5068662059","display_name":"Benjamin A. Kuzma","orcid":"https://orcid.org/0000-0002-9900-8912","observed_orcids":["https://orcid.org/0000-0002-9900-8912"]},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]},{"id":"https://openalex.org/I4210087915","display_name":"Massachusetts General Hospital","ror":"https://ror.org/002pd6e78","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210087915","https://openalex.org/I48633490"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Benjamin A. Kuzma","raw_affiliation_strings":["Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School","institution_ids":["https://openalex.org/I136199984","https://openalex.org/I4210087915"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5054435650","display_name":"Isaac J. Pence","orcid":"https://orcid.org/0000-0002-5635-1374","observed_orcids":["https://orcid.org/0000-0002-5635-1374"]},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]},{"id":"https://openalex.org/I4210087915","display_name":"Massachusetts General Hospital","ror":"https://ror.org/002pd6e78","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210087915","https://openalex.org/I48633490"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Isaac J. Pence","raw_affiliation_strings":["Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School","institution_ids":["https://openalex.org/I136199984","https://openalex.org/I4210087915"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5032311531","display_name":"Alexander Ho","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]},{"id":"https://openalex.org/I4210087915","display_name":"Massachusetts General Hospital","ror":"https://ror.org/002pd6e78","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210087915","https://openalex.org/I48633490"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Alexander Ho","raw_affiliation_strings":["Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School","institution_ids":["https://openalex.org/I136199984","https://openalex.org/I4210087915"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5073936872","display_name":"Conor L. Evans","orcid":"https://orcid.org/0000-0003-2185-6505","observed_orcids":["https://orcid.org/0000-0003-2185-6505"]},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]},{"id":"https://openalex.org/I4210087915","display_name":"Massachusetts General Hospital","ror":"https://ror.org/002pd6e78","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210087915","https://openalex.org/I48633490"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Conor L. Evans","raw_affiliation_strings":["Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School","institution_ids":["https://openalex.org/I136199984","https://openalex.org/I4210087915"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.2272,"has_fulltext":false,"cited_by_count":2,"citation_normalized_percentile":{"value":0.47604252,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":null,"issue":"177","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11324","display_name":"Spectroscopy Techniques in Biomedical and Chemical Research","score":0.9609000086784363,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11324","display_name":"Spectroscopy Techniques in Biomedical and Chemical Research","score":0.9609000086784363,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10704","display_name":"Advancements in Transdermal Drug Delivery","score":0.03700000047683716,"subfield":{"id":"https://openalex.org/subfields/3003","display_name":"Pharmaceutical Science"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10977","display_name":"Optical Imaging and Spectroscopy Techniques","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/2741","display_name":"Radiology, Nuclear Medicine and Imaging"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/topical-bioavailability","display_name":"topical bioavailability","score":0.8669999837875366},{"id":"https://openalex.org/keywords/bioequivalence","display_name":"bioequivalence","score":0.9319999814033508},{"id":"https://openalex.org/keywords/stimulated-raman-scattering","display_name":"stimulated Raman scattering","score":0.9959999918937683},{"id":"https://openalex.org/keywords/coherent-anti-stokes-raman-scattering","display_name":"coherent anti-Stokes Raman scattering","score":0.8769999742507935},{"id":"https://openalex.org/keywords/skin-permeation","display_name":"skin permeation","score":0.5709999799728394},{"id":"https://openalex.org/keywords/human-skin","display_name":"human skin","score":0.7879999876022339},{"id":"https://openalex.org/keywords/mouse-skin","display_name":"mouse skin","score":0.9900000095367432},{"id":"https://openalex.org/keywords/topical-preparations","display_name":"topical formulation","score":0.4699999988079071}],"concepts":[{"id":"https://openalex.org/C45904493","wikidata":"https://www.wikidata.org/wiki/Q912807","display_name":"Active ingredient","level":2,"score":0.6198243498802185},{"id":"https://openalex.org/C169573571","wikidata":"https://www.wikidata.org/wiki/Q466824","display_name":"Raman scattering","level":3,"score":0.5203046798706055},{"id":"https://openalex.org/C2777459323","wikidata":"https://www.wikidata.org/wiki/Q5259911","display_name":"Human skin","level":2,"score":0.47877585887908936},{"id":"https://openalex.org/C136229726","wikidata":"https://www.wikidata.org/wiki/Q327092","display_name":"Biomedical engineering","level":1,"score":0.45224839448928833},{"id":"https://openalex.org/C64903051","wikidata":"https://www.wikidata.org/wiki/Q2198549","display_name":"Drug development","level":3,"score":0.4253287613391876},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.3897967040538788},{"id":"https://openalex.org/C40003534","wikidata":"https://www.wikidata.org/wiki/Q862228","display_name":"Raman spectroscopy","level":2,"score":0.37015026807785034},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.34984374046325684},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.32309427857398987},{"id":"https://openalex.org/C2780035454","wikidata":"https://www.wikidata.org/wiki/Q8386","display_name":"Drug","level":2,"score":0.26423966884613037},{"id":"https://openalex.org/C98274493","wikidata":"https://www.wikidata.org/wiki/Q128406","display_name":"Pharmacology","level":1,"score":0.23863095045089722},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.23459580540657043},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.11960995197296143},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.3791/63264-v","doi":"https://doi.org/10.3791/63264-v","is_oa":false,"landing_page_url":"https://doi.org/10.3791/63264-v","pdf_url":null,"source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.8048999905586243}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W3135129761","https://openalex.org/W4214930379","https://openalex.org/W4324295738","https://openalex.org/W7133589251","https://openalex.org/W4387679346","https://openalex.org/W4214940504","https://openalex.org/W180629573","https://openalex.org/W2969747628","https://openalex.org/W2980457825","https://openalex.org/W4393106483"],"abstract_inverted_index":{"Cutaneous":[0],"pharmacokinetics":[1],"(cPK)":[2],"after":[3],"topical":[4,24,166,205],"formulation":[5],"application":[6],"has":[7,97,158],"been":[8,160],"a":[9,53,184],"research":[10],"area":[11],"of":[12,56,115,204,246],"particular":[13],"interest":[14],"for":[15,90,162,244],"regulatory":[16,179],"and":[17,62,105,112,168,188,207,213,219,228,248],"drug":[18],"development":[19],"scientists":[20],"to":[21,71,124,146,186,193],"mechanistically":[22],"understand":[23],"bioavailability":[25],"(BA).":[26],"Semi-invasive":[27],"techniques,":[28],"such":[29],"as":[30,231,233],"tape-stripping,":[31],"dermal":[32,35],"microdialysis,":[33],"or":[34,93,117,129,149],"open-flow":[36],"microperfusion,":[37],"all":[38],"quantify":[39,147],"macroscale":[40,249],"cPK.":[41],"While":[42],"these":[43,241],"techniques":[44],"have":[45],"provided":[46],"vast":[47],"cPK":[48,74,163],"knowledge,":[49],"the":[50,65,88,135,202],"community":[51],"lacks":[52],"mechanistic":[54],"understanding":[55],"active":[57],"pharmaceutical":[58],"ingredient":[59],"(API)":[60],"penetration":[61],"permeation":[63],"at":[64],"cellular":[66],"level.":[67],"One":[68],"noninvasive":[69],"approach":[70],"address":[72],"microscale":[73],"is":[75,121,139,144],"coherent":[76],"Raman":[77,102,107],"scattering":[78,103,108],"imaging":[79],"(CRI),":[80],"which":[81,138],"selectively":[82],"targets":[83],"intrinsic":[84],"molecular":[85,142],"vibrations":[86],"without":[87],"need":[89],"extrinsic":[91],"labels":[92],"chemical":[94,131],"modification.":[95],"CRI":[96,199],"two":[98],"main":[99],"methods-coherent":[100],"anti-Stokes":[101],"(CARS)":[104],"stimulated":[106],"(SRS)-that":[109],"enable":[110],"sensitive":[111],"selective":[113],"quantification":[114,216],"APIs":[116,148],"inactive":[118,150],"ingredients.":[119],"CARS":[120],"typically":[122],"utilized":[123,161,243],"derive":[125],"structural":[126],"skin":[127,153,192,221],"information":[128],"visualize":[130],"contrast.":[132],"In":[133],"contrast,":[134],"SRS":[136],"signal,":[137],"linear":[140],"with":[141,164],"concentration,":[143],"used":[145,195],"ingredients":[151],"within":[152,217,226],"stratifications.":[154],"Although":[155],"mouse":[156,220],"tissue":[157,177],"commonly":[159],"CRI,":[165],"BA":[167,206,250],"bioequivalence":[169],"(BE)":[170],"must":[171],"ultimately":[172],"be":[173,194],"assessed":[174],"in":[175,196,201],"human":[176,218],"before":[178],"approval.":[180],"This":[181,209],"paper":[182],"presents":[183],"methodology":[185,210],"prepare":[187],"image":[189],"ex":[190],"vivo":[191],"quantitative":[197],"pharmacokinetic":[198],"studies":[200],"evaluation":[203],"BE.":[208,253],"enables":[211],"reliable":[212],"reproducible":[214],"API":[215,235],"over":[222,237],"time.":[223],"The":[224],"concentrations":[225],"lipid-rich":[227],"lipid-poor":[229],"compartments,":[230],"well":[232],"total":[234],"concentration":[236],"time":[238],"are":[239,242],"quantified;":[240],"estimates":[245],"micro-":[247],"and,":[251],"potentially,":[252]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1}],"updated_date":"2026-08-06T08:24:18.245995","created_date":"2022-07-25T00:00:00"}