{"id":"https://openalex.org/W4286826985","doi":"https://doi.org/10.3791/63940-v","title":"Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry","display_name":"Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry","publication_year":2022,"publication_date":"2022-04-21","ids":{"openalex":"https://openalex.org/W4286826985","doi":"https://doi.org/10.3791/63940-v"},"language":"en","primary_location":{"id":"doi:10.3791/63940-v","doi":"https://doi.org/10.3791/63940-v","is_oa":true,"landing_page_url":"https://doi.org/10.3791/63940-v","pdf_url":"https://www.jove.com/pdf/63940/characterizing-rna-modifications-single-neurons-using-mass","source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://www.jove.com/pdf/63940/characterizing-rna-modifications-single-neurons-using-mass","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5068277415","display_name":"Kevin D. Clark","orcid":"https://orcid.org/0000-0002-0576-7716","observed_orcids":["https://orcid.org/0000-0002-0576-7716"]},"institutions":[{"id":"https://openalex.org/I157725225","display_name":"University of Illinois Urbana-Champaign","ror":"https://ror.org/047426m28","country_code":"US","type":"education","lineage":["https://openalex.org/I157725225"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kevin D. Clark","raw_affiliation_strings":["Beckman Institute for Advanced Science and Technology, University of Illinois Urbana-Champaign","Department of Chemistry, University of Illinois Urbana-Champaign"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Beckman Institute for Advanced Science and Technology, University of Illinois Urbana-Champaign","institution_ids":["https://openalex.org/I157725225"]},{"raw_affiliation_string":"Department of Chemistry, University of Illinois Urbana-Champaign","institution_ids":["https://openalex.org/I157725225"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5020098405","display_name":"Stanislav S. Rubakhin","orcid":"https://orcid.org/0000-0003-0437-1493","observed_orcids":["https://orcid.org/0000-0003-0437-1493"]},"institutions":[{"id":"https://openalex.org/I157725225","display_name":"University of Illinois Urbana-Champaign","ror":"https://ror.org/047426m28","country_code":"US","type":"education","lineage":["https://openalex.org/I157725225"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Stanislav S. Rubakhin","raw_affiliation_strings":["Beckman Institute for Advanced Science and Technology, University of Illinois Urbana-Champaign","Department of Chemistry, University of Illinois Urbana-Champaign"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Beckman Institute for Advanced Science and Technology, University of Illinois Urbana-Champaign","institution_ids":["https://openalex.org/I157725225"]},{"raw_affiliation_string":"Department of Chemistry, University of Illinois Urbana-Champaign","institution_ids":["https://openalex.org/I157725225"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5006238710","display_name":"Jonathan V. Sweedler","orcid":"https://orcid.org/0000-0003-3107-9922","observed_orcids":["https://orcid.org/0000-0003-3107-9922"]},"institutions":[{"id":"https://openalex.org/I157725225","display_name":"University of Illinois Urbana-Champaign","ror":"https://ror.org/047426m28","country_code":"US","type":"education","lineage":["https://openalex.org/I157725225"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jonathan V. Sweedler","raw_affiliation_strings":["Beckman Institute for Advanced Science and Technology, University of Illinois Urbana-Champaign","Department of Chemistry, University of Illinois Urbana-Champaign"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Beckman Institute for Advanced Science and Technology, University of Illinois Urbana-Champaign","institution_ids":["https://openalex.org/I157725225"]},{"raw_affiliation_string":"Department of Chemistry, University of Illinois Urbana-Champaign","institution_ids":["https://openalex.org/I157725225"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I157725225"],"apc_list":null,"apc_paid":null,"fwci":0.1633,"has_fulltext":true,"cited_by_count":3,"citation_normalized_percentile":{"value":0.35812765,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":null,"issue":"182","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11482","display_name":"RNA modifications and cancer","score":0.9911999702453613,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11482","display_name":"RNA modifications and cancer","score":0.9911999702453613,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12610","display_name":"RNA regulation and disease","score":0.0031999999191612005,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.0017000000225380063,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/rna-modification","display_name":"RNA modification","score":0.9570000171661377},{"id":"https://openalex.org/keywords/post-transcriptional-modification","display_name":"post-transcriptional modification","score":0.8920000195503235},{"id":"https://openalex.org/keywords/epitranscriptomics","display_name":"epitranscriptomics","score":0.9100000262260437},{"id":"https://openalex.org/keywords/single-neuron-analysis","display_name":"single-neuron analysis","score":0.8450000286102295},{"id":"https://openalex.org/keywords/aplysia-californica","display_name":"Aplysia californica","score":0.9049999713897705},{"id":"https://openalex.org/keywords/neuronal-heterogeneity","display_name":"neuronal heterogeneity","score":0.6190000176429749},{"id":"https://openalex.org/keywords/translational-control","display_name":"translational regulation","score":0.7059999704360962},{"id":"https://openalex.org/keywords/learning-and-memory","display_name":"learning and memory","score":0.781000018119812},{"id":"https://openalex.org/keywords/central-nervous-system","display_name":"central nervous system","score":0.6819999814033508}],"concepts":[{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.8672810196876526},{"id":"https://openalex.org/C2778178740","wikidata":"https://www.wikidata.org/wiki/Q136564","display_name":"Aplysia","level":2,"score":0.7274625897407532},{"id":"https://openalex.org/C162356407","wikidata":"https://www.wikidata.org/wiki/Q180809","display_name":"Mass spectrometry","level":2,"score":0.5595452785491943},{"id":"https://openalex.org/C2778794669","wikidata":"https://www.wikidata.org/wiki/Q43054","display_name":"Neuron","level":2,"score":0.5412229299545288},{"id":"https://openalex.org/C112190416","wikidata":"https://www.wikidata.org/wiki/Q2124450","display_name":"RNA extraction","level":4,"score":0.5199236273765564},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5080161094665527},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.41800305247306824},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.4132278561592102},{"id":"https://openalex.org/C169760540","wikidata":"https://www.wikidata.org/wiki/Q207011","display_name":"Neuroscience","level":1,"score":0.3924393951892853},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.38761472702026367},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.356173038482666},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3414158821105957},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.259914755821228},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.2568882405757904},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1206003725528717}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.3791/63940-v","doi":"https://doi.org/10.3791/63940-v","is_oa":true,"landing_page_url":"https://doi.org/10.3791/63940-v","pdf_url":"https://www.jove.com/pdf/63940/characterizing-rna-modifications-single-neurons-using-mass","source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"}],"best_oa_location":{"id":"doi:10.3791/63940-v","doi":"https://doi.org/10.3791/63940-v","is_oa":true,"landing_page_url":"https://doi.org/10.3791/63940-v","pdf_url":"https://www.jove.com/pdf/63940/characterizing-rna-modifications-single-neurons-using-mass","source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"id":"https://metadata.un.org/sdg/14","display_name":"Life below water","score":0.8799999952316284}],"study_designs":[],"awards":[{"id":"https://openalex.org/G628250572","display_name":"Center for Sub-Cellular Genomics","funder_award_id":"RM1HG010023","funder_id":"https://openalex.org/F4320337348","funder_display_name":"National Human Genome Research Institute"},{"id":"https://openalex.org/G9000863082","display_name":"Neurometabolomics and Neuroproteomics Center on Cell-Cell Signaling","funder_award_id":"P30DA018310","funder_id":"https://openalex.org/F4320337347","funder_display_name":"National Institute on Drug Abuse"}],"funders":[{"id":"https://openalex.org/F4320337347","display_name":"National Institute on Drug Abuse","ror":"https://ror.org/00fq5cm18"},{"id":"https://openalex.org/F4320337348","display_name":"National Human Genome Research Institute","ror":"https://ror.org/00baak391"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4286826985.pdf","grobid_xml":"https://content.openalex.org/works/W4286826985.grobid-xml"},"referenced_works_count":27,"referenced_works":["https://openalex.org/W46083390","https://openalex.org/W1542613299","https://openalex.org/W1927707188","https://openalex.org/W1989762673","https://openalex.org/W2040485185","https://openalex.org/W2058020201","https://openalex.org/W2071930821","https://openalex.org/W2081412654","https://openalex.org/W2091198609","https://openalex.org/W2139357174","https://openalex.org/W2167616678","https://openalex.org/W2176803664","https://openalex.org/W2202722181","https://openalex.org/W2208228295","https://openalex.org/W2252643587","https://openalex.org/W2282095422","https://openalex.org/W2528438861","https://openalex.org/W2612048558","https://openalex.org/W2785337184","https://openalex.org/W2805826733","https://openalex.org/W2884610728","https://openalex.org/W2909556667","https://openalex.org/W2915489297","https://openalex.org/W2952723565","https://openalex.org/W3048442470","https://openalex.org/W3125180351","https://openalex.org/W4239485022"],"related_works":["https://openalex.org/W3205938105","https://openalex.org/W4321996649","https://openalex.org/W7160735785","https://openalex.org/W3048442470","https://openalex.org/W2176803664","https://openalex.org/W4312253926","https://openalex.org/W1903458122","https://openalex.org/W2069815887","https://openalex.org/W4412601202","https://openalex.org/W6969052808"],"abstract_inverted_index":{"Post-transcriptional":[0],"modifications":[1,28,52,215],"(PTMs)":[2],"of":[3,13,40,47,59,105,118,143,150,201,213],"RNA":[4,27,51,81,156,163,184,214],"represent":[5],"an":[6,77,158,173],"understudied":[7],"mechanism":[8],"involved":[9],"in":[10,15,53,95,146,219],"the":[11,16,38,57,70,106,144,210],"regulation":[12],"translation":[14],"central":[17],"nervous":[18],"system":[19],"(CNS).":[20],"Recent":[21],"evidence":[22],"has":[23],"linked":[24],"specific":[25],"neuronal":[26,220],"to":[29,122,154,182],"learning":[30],"and":[31,90,115,135,140,170,198,203],"memory":[32],"paradigms.":[33],"Unfortunately,":[34],"conventional":[35],"methods":[36],"for":[37,66,161,187],"detection":[39],"these":[41],"epitranscriptomic":[42],"features":[43],"are":[44,167,206],"only":[45],"capable":[46],"characterizing":[48],"highly":[49],"abundant":[50],"bulk":[54],"tissues,":[55],"precluding":[56],"assessment":[58],"unique":[60],"PTM":[61],"profiles":[62],"that":[63,194,208],"may":[64],"exist":[65],"individual":[67,103,159,217],"neurons":[68,104,190,218],"within":[69],"activated":[71],"behavioral":[72],"circuits.":[73],"In":[74],"this":[75],"protocol,":[76],"approach":[78,99],"is":[79,100,152,180],"described\u2014single-neuron":[80],"modification":[82,185],"analysis":[83],"by":[84,128],"mass":[85],"spectrometry":[86,177],"(SNRMA-MS)\u2014to":[87],"simultaneously":[88],"detect":[89],"quantify":[91],"numerous":[92],"modified":[93],"ribonucleosides":[94],"single":[96],"neurons.":[97],"The":[98],"validated":[101],"using":[102,132,172],"marine":[107],"mollusk,":[108],"Aplysia":[109],"californica,":[110],"beginning":[111],"with":[112],"surgical":[113],"isolation":[114,131],"enzymatic":[116],"treatment":[117,142],"major":[119],"CNS":[120],"ganglia":[121],"expose":[123],"neuron":[124],"cell":[125,160],"bodies,":[126],"followed":[127],"manual":[129],"single-neuron":[130],"sharp":[133],"needles":[134],"a":[136,147],"micropipette.":[137],"Next,":[138],"mechanical":[139],"thermal":[141],"sample":[145],"small":[148],"volume":[149],"buffer":[151],"done":[153],"liberate":[155],"from":[157,191],"subsequent":[162],"digestion.":[164],"Modified":[165],"nucleosides":[166],"then":[168],"identified":[169,189],"quantified":[171],"optimized":[174],"liquid":[175],"chromatography-mass":[176],"method.":[178],"SNRMA-MS":[179,205],"employed":[181],"establish":[183],"patterns":[186],"single,":[188],"A.":[192],"californica":[193],"have":[195],"known":[196],"morphologies":[197],"functions.":[199],"Examples":[200],"qualitative":[202],"quantitative":[204],"presented":[207],"highlight":[209],"heterogeneous":[211],"distribution":[212],"across":[216],"networks.":[221]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1}],"updated_date":"2026-10-28T10:33:24.094632","created_date":"2022-07-24T00:00:00"}