{"id":"https://openalex.org/W4286587155","doi":"https://doi.org/10.1021/acs.analchem.2c02685","title":"Programmable Ligation-Transcription Circuit-Driven Cascade Amplification Machinery for Multiple Long Noncoding RNAs Detection in Lung Tissues","display_name":"Programmable Ligation-Transcription Circuit-Driven Cascade Amplification Machinery for Multiple Long Noncoding RNAs Detection in Lung Tissues","publication_year":2022,"publication_date":"2022-07-22","ids":{"openalex":"https://openalex.org/W4286587155","doi":"https://doi.org/10.1021/acs.analchem.2c02685","pmid":"https://pubmed.ncbi.nlm.nih.gov/35867839"},"language":"en","primary_location":{"id":"doi:10.1021/acs.analchem.2c02685","doi":"https://doi.org/10.1021/acs.analchem.2c02685","is_oa":false,"landing_page_url":"https://doi.org/10.1021/acs.analchem.2c02685","pdf_url":null,"source":{"id":"https://openalex.org/S191963794","display_name":"Analytical Chemistry","issn_l":"0003-2700","issn":["0003-2700","1520-6882"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Chemistry","raw_type":"journal-article"},"volume":null,"type":"editorial","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5055047430","display_name":"Yan Zhang","orcid":"https://orcid.org/0000-0002-7793-072X","observed_orcids":["https://orcid.org/0000-0002-7793-072X"]},"institutions":[{"id":"https://openalex.org/I28006308","display_name":"Shandong Normal University","ror":"https://ror.org/01wy3h363","country_code":"CN","type":"education","lineage":["https://openalex.org/I28006308"]},{"id":"https://openalex.org/I4210097715","display_name":"Qilu Normal University","ror":"https://ror.org/00wztsq19","country_code":"CN","type":"education","lineage":["https://openalex.org/I4210097715"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yan Zhang","raw_affiliation_strings":["College of Chemistry and Chemical Engineering, Qilu Normal University, Jinan 250200, China","College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Chemistry and Chemical Engineering, Qilu Normal University, Jinan 250200, China","institution_ids":["https://openalex.org/I4210097715"]},{"raw_affiliation_string":"College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, China","institution_ids":["https://openalex.org/I28006308"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5061981045","display_name":"Xueke Du","orcid":"https://orcid.org/0000-0001-9796-9202","observed_orcids":["https://orcid.org/0000-0001-9796-9202"]},"institutions":[{"id":"https://openalex.org/I28006308","display_name":"Shandong Normal University","ror":"https://ror.org/01wy3h363","country_code":"CN","type":"education","lineage":["https://openalex.org/I28006308"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Xue-ke Du","raw_affiliation_strings":["College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, China","institution_ids":["https://openalex.org/I28006308"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100378304","display_name":"Wenjing Liu","orcid":"https://orcid.org/0009-0005-1720-846X","observed_orcids":["https://orcid.org/0009-0005-1720-846X"]},"institutions":[{"id":"https://openalex.org/I76569877","display_name":"Southeast University","ror":"https://ror.org/04ct4d772","country_code":"CN","type":"education","lineage":["https://openalex.org/I76569877"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Wen-jing Liu","raw_affiliation_strings":["School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China","institution_ids":["https://openalex.org/I76569877"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100457561","display_name":"Meng Liu","orcid":"https://orcid.org/0000-0003-2513-7866","observed_orcids":["https://orcid.org/0000-0003-2513-7866"]},"institutions":[{"id":"https://openalex.org/I28006308","display_name":"Shandong Normal University","ror":"https://ror.org/01wy3h363","country_code":"CN","type":"education","lineage":["https://openalex.org/I28006308"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Meng Liu","raw_affiliation_strings":["College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, China","institution_ids":["https://openalex.org/I28006308"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5100602553","display_name":"Chun\u2010yang Zhang","orcid":"https://orcid.org/0000-0002-8010-1981","observed_orcids":["https://orcid.org/0000-0002-8010-1981"]},"institutions":[{"id":"https://openalex.org/I28006308","display_name":"Shandong Normal University","ror":"https://ror.org/01wy3h363","country_code":"CN","type":"education","lineage":["https://openalex.org/I28006308"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Chun-yang Zhang","raw_affiliation_strings":["College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, China"],"raw_orcid":"https://orcid.org/0000-0002-8010-1981","affiliations":[{"raw_affiliation_string":"College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, China","institution_ids":["https://openalex.org/I28006308"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5100602553"],"corresponding_institution_ids":["https://openalex.org/I28006308"],"apc_list":{"value":5000,"currency":"USD","value_usd":5000},"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":16,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"94","issue":"30","first_page":"10573","last_page":"10578"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.9765999913215637,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.9765999913215637,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10515","display_name":"Cancer-related molecular mechanisms research","score":0.009600000455975533,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.003800000064074993,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/long-non-coding-rna","display_name":"long non-coding RNA","score":0.9409999847412109},{"id":"https://openalex.org/keywords/cascade-amplification","display_name":"cascade amplification","score":0.9559999704360962},{"id":"https://openalex.org/keywords/single-molecule-counting","display_name":"single-molecule counting","score":0.8939999938011169},{"id":"https://openalex.org/keywords/hotair","display_name":"HOTAIR","score":0.8939999938011169},{"id":"https://openalex.org/keywords/malat1","display_name":"MALAT1","score":1.0},{"id":"https://openalex.org/keywords/duplex-specific-nuclease","display_name":"duplex-specific nuclease","score":0.9580000042915344},{"id":"https://openalex.org/keywords/magnetic-separation","display_name":"magnetic separation","score":0.6259999871253967},{"id":"https://openalex.org/keywords/non-small-cell-lung-cancer","display_name":"non-small cell lung cancer","score":0.7770000100135803},{"id":"https://openalex.org/keywords/lung-adenocarcinoma","display_name":"lung adenocarcinoma","score":0.8259999752044678},{"id":"https://openalex.org/keywords/single-cell-expression-profiling","display_name":"single-cell expression profiling","score":0.609000027179718}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5846419930458069},{"id":"https://openalex.org/C62203573","wikidata":"https://www.wikidata.org/wiki/Q15087973","display_name":"Long non-coding RNA","level":4,"score":0.5723428130149841},{"id":"https://openalex.org/C2776243700","wikidata":"https://www.wikidata.org/wiki/Q7252869","display_name":"Proximity ligation assay","level":3,"score":0.5151192545890808},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.4715186357498169},{"id":"https://openalex.org/C179926584","wikidata":"https://www.wikidata.org/wiki/Q207714","display_name":"Transcription (linguistics)","level":2,"score":0.4686267673969269},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4219144284725189},{"id":"https://openalex.org/C119203544","wikidata":"https://www.wikidata.org/wiki/Q425891","display_name":"Molecular beacon","level":4,"score":0.41913801431655884},{"id":"https://openalex.org/C2781128886","wikidata":"https://www.wikidata.org/wiki/Q18062498","display_name":"HOTAIR","level":5,"score":0.41562044620513916},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.40558600425720215},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.38621461391448975},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.3323366641998291},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2900218367576599},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.25632303953170776},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.12525320053100586},{"id":"https://openalex.org/C129312508","wikidata":"https://www.wikidata.org/wiki/Q133686","display_name":"Oligonucleotide","level":3,"score":0.0},{"id":"https://openalex.org/C41895202","wikidata":"https://www.wikidata.org/wiki/Q8162","display_name":"Linguistics","level":1,"score":0.0},{"id":"https://openalex.org/C138885662","wikidata":"https://www.wikidata.org/wiki/Q5891","display_name":"Philosophy","level":0,"score":0.0},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D002289","descriptor_name":"Carcinoma, Non-Small-Cell Lung","qualifier_ui":"Q000175","qualifier_name":"diagnosis","is_major_topic":true},{"descriptor_ui":"D002289","descriptor_name":"Carcinoma, Non-Small-Cell Lung","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008168","descriptor_name":"Lung","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008175","descriptor_name":"Lung Neoplasms","qualifier_ui":"Q000175","qualifier_name":"diagnosis","is_major_topic":true},{"descriptor_ui":"D008175","descriptor_name":"Lung Neoplasms","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D062085","descriptor_name":"RNA, Long Noncoding","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1021/acs.analchem.2c02685","doi":"https://doi.org/10.1021/acs.analchem.2c02685","is_oa":false,"landing_page_url":"https://doi.org/10.1021/acs.analchem.2c02685","pdf_url":null,"source":{"id":"https://openalex.org/S191963794","display_name":"Analytical Chemistry","issn_l":"0003-2700","issn":["0003-2700","1520-6882"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Chemistry","raw_type":"journal-article"},{"id":"pmid:35867839","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/35867839","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical chemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.6240000128746033,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G7917761756","display_name":"\u9776\u5411\u80ba\u764c\u5faa\u73af\u80bf\u7624\u7ec6\u80de\u7684\u5355\u5206\u5b50\u68c0\u6d4b\u6280\u672f\u7814\u7a76","funder_award_id":"21735003","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"}],"funders":[{"id":"https://openalex.org/F4320321001","display_name":"National Natural Science Foundation of China","ror":"https://ror.org/01h0zpd94"},{"id":"https://openalex.org/F4320338074","display_name":"Government of Shandong Province","ror":"https://ror.org/03jjt8440"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":37,"referenced_works":["https://openalex.org/W1727677173","https://openalex.org/W1992741792","https://openalex.org/W2004271888","https://openalex.org/W2015310018","https://openalex.org/W2024628274","https://openalex.org/W2030047242","https://openalex.org/W2040206084","https://openalex.org/W2052741109","https://openalex.org/W2053220435","https://openalex.org/W2078653411","https://openalex.org/W2087616533","https://openalex.org/W2096071764","https://openalex.org/W2160728314","https://openalex.org/W2313356495","https://openalex.org/W2322010009","https://openalex.org/W2502698579","https://openalex.org/W2514632341","https://openalex.org/W2517162516","https://openalex.org/W2570812412","https://openalex.org/W2785861340","https://openalex.org/W2796484834","https://openalex.org/W2885929794","https://openalex.org/W2913681910","https://openalex.org/W2943695944","https://openalex.org/W2945791486","https://openalex.org/W2946035471","https://openalex.org/W2954947329","https://openalex.org/W2960638069","https://openalex.org/W2979488527","https://openalex.org/W2993820226","https://openalex.org/W3013409109","https://openalex.org/W3154669964","https://openalex.org/W3158535279","https://openalex.org/W3164698711","https://openalex.org/W3177371019","https://openalex.org/W3183849717","https://openalex.org/W4200070993"],"related_works":["https://openalex.org/W4416416916","https://openalex.org/W4317840358","https://openalex.org/W7128597235","https://openalex.org/W4381194528","https://openalex.org/W3158535279","https://openalex.org/W2945791486","https://openalex.org/W4407378454","https://openalex.org/W4393385669","https://openalex.org/W7155533517","https://openalex.org/W4386258556"],"abstract_inverted_index":{"The":[0,39,58],"measurement":[1,161],"of":[2,13,41,66,114,130,133,162,176,183],"long":[3],"noncoding":[4],"RNAs":[5,60],"(lncRNAs)":[6],"is":[7],"essential":[8],"to":[9,25,52,72,102],"diagnosis":[10,202],"and":[11,36,45,75,84,144,170,190,203],"treatment":[12],"various":[14,177],"diseases":[15],"such":[16],"as":[17],"cancers.":[18],"Herein,":[19],"we":[20],"develop":[21],"a":[22,95,128,197],"simple":[23],"method":[24,122,158],"simultaneously":[26],"detect":[27],"multiple":[28,163],"lncRNAs":[29,165],"using":[30],"programmable":[31],"ligation-transcription":[32,50,100],"circuit-driven":[33],"cascade":[34],"amplification":[35],"single-molecule":[37,90,115],"counting.":[38,91],"presence":[40],"targets":[42],"lncRNA":[43,46,97,178],"HOTAIR":[44],"MALAT1":[47],"activates":[48],"the":[49,64,81,118,167,174],"circuits":[51],"produce":[53],"two":[54],"corresponding":[55,192],"functional":[56,59,105],"RNAs.":[57],"then":[61],"cyclically":[62],"initiate":[63],"digestion":[65],"signal":[67],"probes":[68],"by":[69,89],"duplex-specific":[70],"nuclease":[71],"liberate":[73],"Cy5":[74,83],"Cy3":[76,85],"molecules.":[77],"After":[78],"magnetic":[79],"separation,":[80],"liberated":[82],"molecules":[86],"are":[87],"measured":[88],"In":[92],"this":[93,108,157],"assay,":[94],"single":[96],"can":[98],"activate":[99],"circuit":[101],"generate":[103],"abundant":[104],"RNAs,":[106],"endowing":[107],"assay":[109],"with":[110,127],"high":[111,119,125],"sensitivity.":[112,120],"Integration":[113],"counting":[116],"ensures":[117],"This":[121],"shows":[123],"extremely":[124],"sensitivity":[126],"limit":[129],"detection":[131],"(LOD)":[132],"0.043":[134],"aM":[135,146],"for":[136,147,200],"HOX":[137],"gene":[138],"antisense":[139],"intergenic":[140],"RNA":[141],"(lncRNA":[142,154],"HOTAIR)":[143],"0.126":[145],"mammalian":[148],"metastasis-related":[149],"lung":[150,180,186],"adenocarcinoma":[151],"transcript":[152],"1":[153],"MALAT1).":[155],"Importantly,":[156],"enables":[159],"simultaneous":[160],"endogenous":[164],"at":[166],"single-cell":[168],"level,":[169],"it":[171],"may":[172],"discriminate":[173],"expressions":[175],"in":[179],"tumor":[181],"tissues":[182],"nonsmall":[184],"cell":[185],"cancer":[187],"(NSCLC)":[188],"patients":[189],"their":[191],"healthy":[193],"adjacent":[194],"tissues,":[195],"offering":[196],"promising":[198],"platform":[199],"clinical":[201],"biomedical":[204],"research.":[205]},"counts_by_year":[{"year":2026,"cited_by_count":3},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":5},{"year":2023,"cited_by_count":7}],"updated_date":"2026-11-03T10:50:19.383937","created_date":"2022-07-22T00:00:00"}