{"id":"https://openalex.org/W4285795094","doi":"https://doi.org/10.3389/fvets.2022.894075","title":"Culture of Kenyan Goat (Capra hircus) Undifferentiated Spermatogonia in Feeder-Free Conditions","display_name":"Culture of Kenyan Goat (Capra hircus) Undifferentiated Spermatogonia in Feeder-Free Conditions","publication_year":2022,"publication_date":"2022-07-19","ids":{"openalex":"https://openalex.org/W4285795094","doi":"https://doi.org/10.3389/fvets.2022.894075","pmid":"https://pubmed.ncbi.nlm.nih.gov/35928111","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/9343694"},"language":"en","primary_location":{"id":"doi:10.3389/fvets.2022.894075","doi":"https://doi.org/10.3389/fvets.2022.894075","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fvets.2022.894075","pdf_url":"https://www.frontiersin.org/articles/10.3389/fvets.2022.894075/pdf","source":{"id":"https://openalex.org/S2594976040","display_name":"Frontiers in Veterinary Science","issn_l":"2297-1769","issn":["2297-1769"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Veterinary Science","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","datacite","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.frontiersin.org/articles/10.3389/fvets.2022.894075/pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5075410742","display_name":"Wilkister Nakami","orcid":"https://orcid.org/0000-0003-3604-0234","observed_orcids":["https://orcid.org/0000-0003-3604-0234"]},"institutions":[{"id":"https://openalex.org/I12859529","display_name":"University of Nairobi","ror":"https://ror.org/02y9nww90","country_code":"KE","type":"education","lineage":["https://openalex.org/I12859529"]},{"id":"https://openalex.org/I58422712","display_name":"International Livestock Research Institute","ror":"https://ror.org/01jxjwb74","country_code":"KE","type":"facility","lineage":["https://openalex.org/I1286583668","https://openalex.org/I58422712"]}],"countries":["KE"],"is_corresponding":true,"raw_author_name":"Nakami Wilkister Nabulindo","raw_affiliation_strings":["Centre for Tropical Livestock Genetics and Health Laboratory, International Livestock Research Institute, Nairobi, Kenya","Department of Clinical Studies, Faculty of Veterinary Medicine, University of Nairobi, Nairobi, Kenya"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centre for Tropical Livestock Genetics and Health Laboratory, International Livestock Research Institute, Nairobi, Kenya","institution_ids":["https://openalex.org/I58422712"]},{"raw_affiliation_string":"Department of Clinical Studies, Faculty of Veterinary Medicine, University of Nairobi, Nairobi, Kenya","institution_ids":["https://openalex.org/I12859529"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5023780953","display_name":"James Nguhiu-Mwangi","orcid":"https://orcid.org/0000-0001-5052-8696","observed_orcids":["https://orcid.org/0000-0001-5052-8696"]},"institutions":[{"id":"https://openalex.org/I12859529","display_name":"University of Nairobi","ror":"https://ror.org/02y9nww90","country_code":"KE","type":"education","lineage":["https://openalex.org/I12859529"]}],"countries":["KE"],"is_corresponding":false,"raw_author_name":"James Nguhiu-Mwangi","raw_affiliation_strings":["Department of Clinical Studies, Faculty of Veterinary Medicine, University of Nairobi, Nairobi, Kenya"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Clinical Studies, Faculty of Veterinary Medicine, University of Nairobi, Nairobi, Kenya","institution_ids":["https://openalex.org/I12859529"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5068258748","display_name":"Ambrose Ng\u2019eno Kipyegon","orcid":"https://orcid.org/0000-0001-9467-6201","observed_orcids":["https://orcid.org/0000-0001-9467-6201"]},"institutions":[{"id":"https://openalex.org/I12859529","display_name":"University of Nairobi","ror":"https://ror.org/02y9nww90","country_code":"KE","type":"education","lineage":["https://openalex.org/I12859529"]}],"countries":["KE"],"is_corresponding":false,"raw_author_name":"Ambrose Ng'eno Kipyegon","raw_affiliation_strings":["Department of Clinical Studies, Faculty of Veterinary Medicine, University of Nairobi, Nairobi, Kenya"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Clinical Studies, Faculty of Veterinary Medicine, University of Nairobi, Nairobi, Kenya","institution_ids":["https://openalex.org/I12859529"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5084479199","display_name":"Moses Ogugo","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I58422712","display_name":"International Livestock Research Institute","ror":"https://ror.org/01jxjwb74","country_code":"KE","type":"facility","lineage":["https://openalex.org/I1286583668","https://openalex.org/I58422712"]}],"countries":["KE"],"is_corresponding":false,"raw_author_name":"Moses Ogugo","raw_affiliation_strings":["Centre for Tropical Livestock Genetics and Health Laboratory, International Livestock Research Institute, Nairobi, Kenya"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centre for Tropical Livestock Genetics and Health Laboratory, International Livestock Research Institute, Nairobi, Kenya","institution_ids":["https://openalex.org/I58422712"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5037266704","display_name":"Charity Muteti","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I58422712","display_name":"International Livestock Research Institute","ror":"https://ror.org/01jxjwb74","country_code":"KE","type":"facility","lineage":["https://openalex.org/I1286583668","https://openalex.org/I58422712"]}],"countries":["KE"],"is_corresponding":false,"raw_author_name":"Charity Muteti","raw_affiliation_strings":["Centre for Tropical Livestock Genetics and Health Laboratory, International Livestock Research Institute, Nairobi, Kenya"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centre for Tropical Livestock Genetics and Health Laboratory, International Livestock Research Institute, Nairobi, Kenya","institution_ids":["https://openalex.org/I58422712"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5041897099","display_name":"Tiambo Christian","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I58422712","display_name":"International Livestock Research Institute","ror":"https://ror.org/01jxjwb74","country_code":"KE","type":"facility","lineage":["https://openalex.org/I1286583668","https://openalex.org/I58422712"]}],"countries":["KE"],"is_corresponding":false,"raw_author_name":"Tiambo Christian","raw_affiliation_strings":["Centre for Tropical Livestock Genetics and Health Laboratory, International Livestock Research Institute, Nairobi, Kenya"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centre for Tropical Livestock Genetics and Health Laboratory, International Livestock Research Institute, Nairobi, Kenya","institution_ids":["https://openalex.org/I58422712"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110664304","display_name":"Melissa J. Oatley","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I72951846","display_name":"Washington State University","ror":"https://ror.org/05dk0ce17","country_code":"US","type":"education","lineage":["https://openalex.org/I72951846"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Melissa J. Oatley","raw_affiliation_strings":["Center for Reproductive Biology, Washington State University, Pullman, WA, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Reproductive Biology, Washington State University, Pullman, WA, United States","institution_ids":["https://openalex.org/I72951846"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5014447224","display_name":"Jon M. Oatley","orcid":"https://orcid.org/0000-0001-6692-4351","observed_orcids":["https://orcid.org/0000-0001-6692-4351"]},"institutions":[{"id":"https://openalex.org/I72951846","display_name":"Washington State University","ror":"https://ror.org/05dk0ce17","country_code":"US","type":"education","lineage":["https://openalex.org/I72951846"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jon M. Oatley","raw_affiliation_strings":["Center for Reproductive Biology, Washington State University, Pullman, WA, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Reproductive Biology, Washington State University, Pullman, WA, United States","institution_ids":["https://openalex.org/I72951846"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5075819806","display_name":"Stephen J. Kemp","orcid":"https://orcid.org/0000-0003-4041-1720","observed_orcids":["https://orcid.org/0000-0003-4041-1720"]},"institutions":[{"id":"https://openalex.org/I58422712","display_name":"International Livestock Research Institute","ror":"https://ror.org/01jxjwb74","country_code":"KE","type":"facility","lineage":["https://openalex.org/I1286583668","https://openalex.org/I58422712"]}],"countries":["KE"],"is_corresponding":false,"raw_author_name":"Stephen Kemp","raw_affiliation_strings":["Centre for Tropical Livestock Genetics and Health Laboratory, International Livestock Research Institute, Nairobi, Kenya"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centre for Tropical Livestock Genetics and Health Laboratory, International Livestock Research Institute, Nairobi, Kenya","institution_ids":["https://openalex.org/I58422712"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5075410742"],"corresponding_institution_ids":["https://openalex.org/I12859529","https://openalex.org/I58422712"],"apc_list":{"value":2950,"currency":"USD","value_usd":2950},"apc_paid":{"value":2950,"currency":"USD","value_usd":2950},"fwci":1.0534,"has_fulltext":true,"cited_by_count":6,"citation_normalized_percentile":{"value":0.74095099,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":90,"max":97},"biblio":{"volume":"9","issue":null,"first_page":"894075","last_page":"894075"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10116","display_name":"Sperm and Testicular Function","score":0.8637999892234802,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10116","display_name":"Sperm and Testicular Function","score":0.8637999892234802,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12929","display_name":"Animal Genetics and Reproduction","score":0.13449999690055847,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10364","display_name":"Reproductive Biology and Fertility","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/undifferentiated-spermatogonia","display_name":"undifferentiated spermatogonia","score":0.9179999828338623},{"id":"https://openalex.org/keywords/spermatogonial-stem-cells","display_name":"spermatogonial stem cells","score":0.9819999933242798},{"id":"https://openalex.org/keywords/goats","display_name":"goats","score":0.8330000042915344},{"id":"https://openalex.org/keywords/feeder-free-culture","display_name":"feeder-free culture","score":0.8859999775886536},{"id":"https://openalex.org/keywords/serum-free-medium","display_name":"serum-free medium","score":0.8389999866485596},{"id":"https://openalex.org/keywords/germ-cell-transplantation","display_name":"germ cell transplantation","score":0.4830000102519989},{"id":"https://openalex.org/keywords/spermatogenesis","display_name":"spermatogenesis","score":0.6489999890327454},{"id":"https://openalex.org/keywords/vasa","display_name":"Vasa","score":0.7540000081062317},{"id":"https://openalex.org/keywords/plzf","display_name":"PLZF","score":0.996999979019165},{"id":"https://openalex.org/keywords/growth-factor-supplementation","display_name":"growth factor supplementation","score":0.6629999876022339},{"id":"https://openalex.org/keywords/testis","display_name":"testis","score":0.36800000071525574}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.811385452747345},{"id":"https://openalex.org/C2908647359","wikidata":"https://www.wikidata.org/wiki/Q2625603","display_name":"Population","level":2,"score":0.6506265997886658},{"id":"https://openalex.org/C185856081","wikidata":"https://www.wikidata.org/wiki/Q14916286","display_name":"Glial cell line-derived neurotrophic factor","level":4,"score":0.5570691227912903},{"id":"https://openalex.org/C16685009","wikidata":"https://www.wikidata.org/wiki/Q335057","display_name":"Andrology","level":1,"score":0.5277410745620728},{"id":"https://openalex.org/C123765429","wikidata":"https://www.wikidata.org/wiki/Q36961","display_name":"Spermatogenesis","level":2,"score":0.4847242832183838},{"id":"https://openalex.org/C28328180","wikidata":"https://www.wikidata.org/wiki/Q48196","display_name":"Stem cell","level":2,"score":0.4831806719303131},{"id":"https://openalex.org/C2911091166","wikidata":"https://www.wikidata.org/wiki/Q106419912","display_name":"Transplantation","level":2,"score":0.47163888812065125},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.4668842852115631},{"id":"https://openalex.org/C2777587049","wikidata":"https://www.wikidata.org/wiki/Q20969945","display_name":"Basic fibroblast growth factor","level":4,"score":0.4214520752429962},{"id":"https://openalex.org/C207701140","wikidata":"https://www.wikidata.org/wiki/Q420522","display_name":"Homeobox protein NANOG","level":5,"score":0.41795480251312256},{"id":"https://openalex.org/C2775960820","wikidata":"https://www.wikidata.org/wiki/Q408378","display_name":"Growth factor","level":3,"score":0.38333895802497864},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.36863672733306885},{"id":"https://openalex.org/C160539049","wikidata":"https://www.wikidata.org/wiki/Q3064187","display_name":"Neurotrophic factors","level":3,"score":0.35197293758392334},{"id":"https://openalex.org/C145103041","wikidata":"https://www.wikidata.org/wiki/Q1151519","display_name":"Embryonic stem cell","level":3,"score":0.26777398586273193},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.2651174068450928},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.22129300236701965},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.2115224003791809},{"id":"https://openalex.org/C107459253","wikidata":"https://www.wikidata.org/wiki/Q674925","display_name":"Induced pluripotent stem cell","level":4,"score":0.08200523257255554},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.0},{"id":"https://openalex.org/C149923435","wikidata":"https://www.wikidata.org/wiki/Q37732","display_name":"Demography","level":1,"score":0.0},{"id":"https://openalex.org/C144024400","wikidata":"https://www.wikidata.org/wiki/Q21201","display_name":"Sociology","level":0,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.0}],"mesh":[],"locations_count":7,"locations":[{"id":"doi:10.3389/fvets.2022.894075","doi":"https://doi.org/10.3389/fvets.2022.894075","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fvets.2022.894075","pdf_url":"https://www.frontiersin.org/articles/10.3389/fvets.2022.894075/pdf","source":{"id":"https://openalex.org/S2594976040","display_name":"Frontiers in Veterinary Science","issn_l":"2297-1769","issn":["2297-1769"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Veterinary Science","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:9343694","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/9343694","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Front Vet Sci","raw_type":"Text"},{"id":"pmid:35928111","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/35928111","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in veterinary science","raw_type":"Journal Article"},{"id":"pmh:oai:cgspace.cgiar.org:10568/120247","doi":null,"is_oa":true,"landing_page_url":"https://hdl.handle.net/10568/120247","pdf_url":null,"source":{"id":"https://openalex.org/S4306402055","display_name":"CGSPace A Repository of Agricultural Research Outputs (Consultative Group for International Agricultural Research)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1286583668","host_organization_name":"CGIAR","host_organization_lineage":["https://openalex.org/I1286583668"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Nabulindo, N.W., Nguhiu-Mwangi, J., Kipyegon, A.N., Ogugo, M., Muteti, C, Christian, T., Oatley, M.J., Oatley, J.M. and Kemp, Stephen J. 2022. Culture of Kenyan goat (Capra hircus) undifferentiated spermatogonia in feeder-free conditions. Frontiers in Veterinary Science, 9: 894075.","raw_type":"Journal Article"},{"id":"pmh:oai:doaj.org/article:213e17ee9cdf43ed995e7aa5f44e8207","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/213e17ee9cdf43ed995e7aa5f44e8207","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Frontiers in Veterinary Science, Vol 9 (2022)","raw_type":"article"},{"id":"doi:10.60692/0bh8c-94k44","doi":"https://doi.org/10.60692/0bh8c-94k44","is_oa":true,"landing_page_url":"https://doi.org/10.60692/0bh8c-94k44","pdf_url":null,"source":{"id":"https://openalex.org/S7407051682","display_name":"Greater South Information System","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"article-journal"},{"id":"doi:10.60692/whhnm-myv53","doi":"https://doi.org/10.60692/whhnm-myv53","is_oa":true,"landing_page_url":"https://doi.org/10.60692/whhnm-myv53","pdf_url":null,"source":{"id":"https://openalex.org/S7407051682","display_name":"Greater South Information System","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"article-journal"}],"best_oa_location":{"id":"doi:10.3389/fvets.2022.894075","doi":"https://doi.org/10.3389/fvets.2022.894075","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fvets.2022.894075","pdf_url":"https://www.frontiersin.org/articles/10.3389/fvets.2022.894075/pdf","source":{"id":"https://openalex.org/S2594976040","display_name":"Frontiers in Veterinary Science","issn_l":"2297-1769","issn":["2297-1769"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Veterinary Science","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.4359000027179718,"id":"https://metadata.un.org/sdg/2","display_name":"Zero hunger"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320328548","display_name":"International Livestock Research Institute","ror":"https://ror.org/01jxjwb74"},{"id":"https://openalex.org/F4320329182","display_name":"Consortium of International Agricultural Research Centers","ror":"https://ror.org/04c4bm785"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4285795094.pdf","grobid_xml":"https://content.openalex.org/works/W4285795094.grobid-xml"},"referenced_works_count":30,"referenced_works":["https://openalex.org/W246865704","https://openalex.org/W1700092582","https://openalex.org/W2017473812","https://openalex.org/W2021971921","https://openalex.org/W2025551614","https://openalex.org/W2040605613","https://openalex.org/W2045519522","https://openalex.org/W2059033739","https://openalex.org/W2072791055","https://openalex.org/W2110804359","https://openalex.org/W2127264690","https://openalex.org/W2131238404","https://openalex.org/W2139789628","https://openalex.org/W2143065569","https://openalex.org/W2155023059","https://openalex.org/W2168003742","https://openalex.org/W2171929902","https://openalex.org/W2314764226","https://openalex.org/W2408867634","https://openalex.org/W2417670710","https://openalex.org/W2605274597","https://openalex.org/W2791390254","https://openalex.org/W2802672272","https://openalex.org/W2912429831","https://openalex.org/W2940714046","https://openalex.org/W2950223343","https://openalex.org/W3009681692","https://openalex.org/W3020849409","https://openalex.org/W3080503475","https://openalex.org/W3087233205"],"related_works":["https://openalex.org/W2417670710","https://openalex.org/W2593843896","https://openalex.org/W2791390254","https://openalex.org/W2043160076","https://openalex.org/W2772268330","https://openalex.org/W2772093451","https://openalex.org/W2045519522","https://openalex.org/W2072791055","https://openalex.org/W3009681692","https://openalex.org/W2610540826"],"abstract_inverted_index":{"The":[0,124],"undifferentiated":[1,85,102,177,216],"spermatogonial":[2,9,195,217],"population":[3,13,58,218],"in":[4,42,50,59,116,225],"mammalian":[5],"testes":[6,221],"contains":[7],"a":[8,30,57,88,117,136,170,209],"stem":[10],"cell":[11,143,186],"(SSC)":[12],"that":[14,78,91,140],"can":[15],"regenerate":[16],"continual":[17],"spermatogenesis":[18],"following":[19],"transplantation.":[20],"This":[21],"capacity":[22],"has":[23],"the":[24,53,166,184,192,213],"potential":[25],"to":[26,35,55,64,94],"be":[27,62],"exploited":[28],"as":[29,189,191,198],"surrogate":[31,71],"sires":[32],"breeding":[33],"tool":[34],"achieve":[36],"widespread":[37],"dissemination":[38],"of":[39,84,174],"desirable":[40],"genetics":[41],"livestock":[43,96],"production.":[44,97],"Because":[45],"SSCs":[46],"are":[47],"relatively":[48],"rare":[49],"testicular":[51],"tissue,":[52],"ability":[54],"expand":[56],"vitro":[60],"would":[61,79],"advantageous":[63],"provide":[65,208],"large":[66],"numbers":[67],"for":[68,101,211],"transplantation":[69],"into":[70],"recipient":[72],"males.":[73],"Here,":[74],"we":[75],"evaluated":[76],"conditions":[77],"support":[80],"long-term":[81,223],"in-vitro":[82,175],"maintenance":[83,224],"spermatogonia":[86,103,178],"from":[87,104,179,219],"goat":[89,130,220],"breed":[90],"is":[92],"endemic":[93],"Kenyan":[95],"Single-cell":[98],"suspensions":[99],"enriched":[100],"pre-pubertal":[105],"bucks":[106],"were":[107],"seeded":[108],"on":[109,121,129],"laminin-coated":[110],"tissue":[111],"culture":[112,227],"plates":[113],"and":[114,133,156,182,222],"maintained":[115],"commercial":[118],"media":[119,126],"based":[120],"serum-free":[122,125],"composition.":[123],"was":[127],"conditioned":[128],"fetal":[131],"fibroblasts":[132],"supplemented":[134],"with":[135],"growth":[137,158],"factor":[138,146,150,154,159],"cocktail":[139],"included":[141],"glial":[142],"line-derived":[144],"neurotrophic":[145],"(GDNF),":[147],"leukemia":[148,200],"inhibitory":[149],"(LIF),":[151],"stromal":[152],"cell-derived":[153],"(SDF),":[155],"fibroblast":[157],"(FGF)":[160],"before":[161],"use.":[162],"Over":[163],"45":[164],"days,":[165],"primary":[167],"cultures":[168],"developed":[169],"cluster":[171],"morphology":[172],"indicative":[173],"grown":[176],"other":[180],"species":[181],"expressed":[183],"germ":[185],"marker":[187,196],"VASA,":[188],"well":[190],"previously":[193],"defined":[194,226],"such":[197],"promyelocytic":[199],"zinc":[201],"finger":[202],"(PLZF).":[203],"Taken":[204],"together,":[205],"these":[206],"findings":[207],"methodology":[210],"isolating":[212],"SSC":[214],"containing":[215],"conditions.":[228]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":4}],"updated_date":"2026-10-28T10:33:24.094632","created_date":"2022-07-19T00:00:00"}