{"id":"https://openalex.org/W4285048397","doi":"https://doi.org/10.3389/fvets.2022.926881","title":"A Multiplex Crystal Digital PCR for Detection of African Swine Fever Virus, Classical Swine Fever Virus, and Porcine Reproductive and Respiratory Syndrome Virus","display_name":"A Multiplex Crystal Digital PCR for Detection of African Swine Fever Virus, Classical Swine Fever Virus, and Porcine Reproductive and Respiratory Syndrome Virus","publication_year":2022,"publication_date":"2022-06-24","ids":{"openalex":"https://openalex.org/W4285048397","doi":"https://doi.org/10.3389/fvets.2022.926881","pmid":"https://pubmed.ncbi.nlm.nih.gov/35812859","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/9270018"},"language":"en","primary_location":{"id":"doi:10.3389/fvets.2022.926881","doi":"https://doi.org/10.3389/fvets.2022.926881","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fvets.2022.926881","pdf_url":"https://www.frontiersin.org/articles/10.3389/fvets.2022.926881/pdf","source":{"id":"https://openalex.org/S2594976040","display_name":"Frontiers in Veterinary Science","issn_l":"2297-1769","issn":["2297-1769"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Veterinary Science","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.frontiersin.org/articles/10.3389/fvets.2022.926881/pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5005850471","display_name":"Kaichuang Shi","orcid":"https://orcid.org/0000-0002-1023-9635","observed_orcids":["https://orcid.org/0000-0002-1023-9635"]},"institutions":[{"id":"https://openalex.org/I150807315","display_name":"Guangxi University","ror":"https://ror.org/02c9qn167","country_code":"CN","type":"education","lineage":["https://openalex.org/I150807315"]},{"id":"https://openalex.org/I4210143324","display_name":"Guangxi Center for Disease Prevention and Control","ror":"https://ror.org/047a9ch09","country_code":"CN","type":"government","lineage":["https://openalex.org/I4210143324"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Kaichuang Shi","raw_affiliation_strings":["College of Animal Science and Technology, Guangxi University, Nanning, China","Guangxi Center for Animal Disease Control and Prevention, Nanning, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Animal Science and Technology, Guangxi University, Nanning, China","institution_ids":["https://openalex.org/I150807315"]},{"raw_affiliation_string":"Guangxi Center for Animal Disease Control and Prevention, Nanning, China","institution_ids":["https://openalex.org/I4210143324"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101609130","display_name":"Yating Chen","orcid":"https://orcid.org/0000-0002-5716-6269","observed_orcids":["https://orcid.org/0000-0002-5716-6269"]},"institutions":[{"id":"https://openalex.org/I150807315","display_name":"Guangxi University","ror":"https://ror.org/02c9qn167","country_code":"CN","type":"education","lineage":["https://openalex.org/I150807315"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yating Chen","raw_affiliation_strings":["College of Animal Science and Technology, Guangxi University, Nanning, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Animal Science and Technology, Guangxi University, Nanning, China","institution_ids":["https://openalex.org/I150807315"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5005342873","display_name":"Yanwen Yin","orcid":"https://orcid.org/0000-0002-3890-2080","observed_orcids":["https://orcid.org/0000-0002-3890-2080"]},"institutions":[{"id":"https://openalex.org/I4210143324","display_name":"Guangxi Center for Disease Prevention and Control","ror":"https://ror.org/047a9ch09","country_code":"CN","type":"government","lineage":["https://openalex.org/I4210143324"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yanwen Yin","raw_affiliation_strings":["Guangxi Center for Animal Disease Control and Prevention, Nanning, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Guangxi Center for Animal Disease Control and Prevention, Nanning, China","institution_ids":["https://openalex.org/I4210143324"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5056475276","display_name":"Feng Long","orcid":"https://orcid.org/0000-0002-9625-0527","observed_orcids":["https://orcid.org/0000-0002-9625-0527"]},"institutions":[{"id":"https://openalex.org/I4210143324","display_name":"Guangxi Center for Disease Prevention and Control","ror":"https://ror.org/047a9ch09","country_code":"CN","type":"government","lineage":["https://openalex.org/I4210143324"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Feng Long","raw_affiliation_strings":["Guangxi Center for Animal Disease Control and Prevention, Nanning, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Guangxi Center for Animal Disease Control and Prevention, Nanning, China","institution_ids":["https://openalex.org/I4210143324"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5049597309","display_name":"Shuping Feng","orcid":"https://orcid.org/0009-0009-3670-9672","observed_orcids":["https://orcid.org/0009-0009-3670-9672"]},"institutions":[{"id":"https://openalex.org/I4210143324","display_name":"Guangxi Center for Disease Prevention and Control","ror":"https://ror.org/047a9ch09","country_code":"CN","type":"government","lineage":["https://openalex.org/I4210143324"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Shuping Feng","raw_affiliation_strings":["Guangxi Center for Animal Disease Control and Prevention, Nanning, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Guangxi Center for Animal Disease Control and Prevention, Nanning, China","institution_ids":["https://openalex.org/I4210143324"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5107871616","display_name":"Huixin Liu","orcid":"https://orcid.org/0009-0005-1846-4529","observed_orcids":["https://orcid.org/0009-0005-1846-4529"]},"institutions":[{"id":"https://openalex.org/I150807315","display_name":"Guangxi University","ror":"https://ror.org/02c9qn167","country_code":"CN","type":"education","lineage":["https://openalex.org/I150807315"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Huixin Liu","raw_affiliation_strings":["College of Animal Science and Technology, Guangxi University, Nanning, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Animal Science and Technology, Guangxi University, Nanning, China","institution_ids":["https://openalex.org/I150807315"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101188086","display_name":"Sujie Qu","orcid":"https://orcid.org/0000-0002-1600-8248","observed_orcids":["https://orcid.org/0000-0002-1600-8248"]},"institutions":[{"id":"https://openalex.org/I4210143324","display_name":"Guangxi Center for Disease Prevention and Control","ror":"https://ror.org/047a9ch09","country_code":"CN","type":"government","lineage":["https://openalex.org/I4210143324"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Sujie Qu","raw_affiliation_strings":["Guangxi Center for Animal Disease Control and Prevention, Nanning, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Guangxi Center for Animal Disease Control and Prevention, Nanning, China","institution_ids":["https://openalex.org/I4210143324"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5070094506","display_name":"Hongbin Si","orcid":"https://orcid.org/0000-0002-0416-6150","observed_orcids":["https://orcid.org/0000-0002-0416-6150","https://orcid.org/0009-0000-4508-6264"]},"institutions":[{"id":"https://openalex.org/I150807315","display_name":"Guangxi University","ror":"https://ror.org/02c9qn167","country_code":"CN","type":"education","lineage":["https://openalex.org/I150807315"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Hongbin Si","raw_affiliation_strings":["College of Animal Science and Technology, Guangxi University, Nanning, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Animal Science and Technology, Guangxi University, Nanning, China","institution_ids":["https://openalex.org/I150807315"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5005850471","https://openalex.org/A5070094506"],"corresponding_institution_ids":["https://openalex.org/I150807315","https://openalex.org/I4210143324"],"apc_list":{"value":2950,"currency":"USD","value_usd":2950},"apc_paid":{"value":2950,"currency":"USD","value_usd":2950},"fwci":3.6424,"has_fulltext":true,"cited_by_count":28,"citation_normalized_percentile":{"value":0.93149885,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"9","issue":null,"first_page":"926881","last_page":"926881"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11560","display_name":"Animal Disease Management and Epidemiology","score":0.899399995803833,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11560","display_name":"Animal Disease Management and Epidemiology","score":0.899399995803833,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11495","display_name":"Animal Virus Infections Studies","score":0.08030000329017639,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11393","display_name":"Biosensors and Analytical Detection","score":0.004600000102072954,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/african-swine-fever-virus","display_name":"African swine fever virus","score":0.968999981880188},{"id":"https://openalex.org/keywords/classical-swine-fever-virus","display_name":"classical swine fever virus","score":0.9990000128746033},{"id":"https://openalex.org/keywords/porcine-reproductive-and-respiratory-syndrome-virus","display_name":"porcine reproductive and respiratory syndrome virus","score":1.0},{"id":"https://openalex.org/keywords/multiplex-digital-pcr","display_name":"multiplex digital PCR","score":0.871999979019165},{"id":"https://openalex.org/keywords/p72-gene","display_name":"p72 gene","score":0.8849999904632568},{"id":"https://openalex.org/keywords/5-untranslated-region","display_name":"5' untranslated region","score":0.9700000286102295},{"id":"https://openalex.org/keywords/orf7-gene","display_name":"ORF7 gene","score":0.9860000014305115},{"id":"https://openalex.org/keywords/swine-diseases","display_name":"swine diseases","score":0.38199999928474426},{"id":"https://openalex.org/keywords/coinfection","display_name":"co-infection","score":0.6909999847412109},{"id":"https://openalex.org/keywords/clinical-samples","display_name":"clinical samples","score":0.3970000147819519},{"id":"https://openalex.org/keywords/qrt-pcr-comparison","display_name":"qRT-PCR comparison","score":0.6919999718666077},{"id":"https://openalex.org/keywords/assay-sensitivity-and-specificity","display_name":"assay sensitivity and specificity","score":0.5789999961853027}],"concepts":[{"id":"https://openalex.org/C2779819720","wikidata":"https://www.wikidata.org/wiki/Q1760147","display_name":"Porcine reproductive and respiratory syndrome virus","level":3,"score":0.9003312587738037},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.8087559938430786},{"id":"https://openalex.org/C2779005923","wikidata":"https://www.wikidata.org/wiki/Q683770","display_name":"Classical swine fever","level":3,"score":0.7447714805603027},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.6894222497940063},{"id":"https://openalex.org/C57066541","wikidata":"https://www.wikidata.org/wiki/Q4765002","display_name":"Veterinary virology","level":3,"score":0.6851719617843628},{"id":"https://openalex.org/C2778359420","wikidata":"https://www.wikidata.org/wiki/Q195760","display_name":"African swine fever virus","level":3,"score":0.5913386344909668},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.554934024810791},{"id":"https://openalex.org/C90583042","wikidata":"https://www.wikidata.org/wiki/Q1952456","display_name":"Multiplex polymerase chain reaction","level":4,"score":0.4567625820636749},{"id":"https://openalex.org/C534529494","wikidata":"https://www.wikidata.org/wiki/Q7891","display_name":"Respiratory system","level":2,"score":0.427815705537796},{"id":"https://openalex.org/C48023723","wikidata":"https://www.wikidata.org/wiki/Q856198","display_name":"Real-time polymerase chain reaction","level":3,"score":0.41987791657447815},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.3444594144821167},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.13335633277893066},{"id":"https://openalex.org/C105702510","wikidata":"https://www.wikidata.org/wiki/Q514","display_name":"Anatomy","level":1,"score":0.0},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0}],"mesh":[],"locations_count":4,"locations":[{"id":"doi:10.3389/fvets.2022.926881","doi":"https://doi.org/10.3389/fvets.2022.926881","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fvets.2022.926881","pdf_url":"https://www.frontiersin.org/articles/10.3389/fvets.2022.926881/pdf","source":{"id":"https://openalex.org/S2594976040","display_name":"Frontiers in Veterinary Science","issn_l":"2297-1769","issn":["2297-1769"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Veterinary Science","raw_type":"journal-article"},{"id":"pmid:35812859","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/35812859","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in veterinary science","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:9270018","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC9270018/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Front Vet Sci","raw_type":"Text"},{"id":"pmh:oai:doaj.org/article:149639ddbd32497886ee0ffe6460361b","doi":null,"is_oa":false,"landing_page_url":"https://doaj.org/article/149639ddbd32497886ee0ffe6460361b","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Frontiers in Veterinary Science, Vol 9 (2022)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.3389/fvets.2022.926881","doi":"https://doi.org/10.3389/fvets.2022.926881","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fvets.2022.926881","pdf_url":"https://www.frontiersin.org/articles/10.3389/fvets.2022.926881/pdf","source":{"id":"https://openalex.org/S2594976040","display_name":"Frontiers in Veterinary Science","issn_l":"2297-1769","issn":["2297-1769"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Veterinary Science","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.6240000128746033,"display_name":"Good health and well-being"},{"id":"https://metadata.un.org/sdg/2","score":0.5666999816894531,"display_name":"Zero hunger"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G5278177371","display_name":null,"funder_award_id":"AB21238003","funder_id":"https://openalex.org/F4320336589","funder_display_name":"Guangxi Key Research and Development Program"},{"id":"https://openalex.org/G6021516157","display_name":null,"funder_award_id":"AA17204057","funder_id":"https://openalex.org/F4320335965","funder_display_name":"Science and Technology Major Project of Guangxi"}],"funders":[{"id":"https://openalex.org/F4320335965","display_name":"Science and Technology Major Project of Guangxi","ror":null},{"id":"https://openalex.org/F4320336589","display_name":"Guangxi Key Research and Development Program","ror":null}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4285048397.pdf","grobid_xml":"https://content.openalex.org/works/W4285048397.grobid-xml"},"referenced_works_count":37,"referenced_works":["https://openalex.org/W1967778241","https://openalex.org/W1968017175","https://openalex.org/W1982838264","https://openalex.org/W1990524275","https://openalex.org/W2011571583","https://openalex.org/W2036522696","https://openalex.org/W2042194227","https://openalex.org/W2047043493","https://openalex.org/W2064543601","https://openalex.org/W2083506607","https://openalex.org/W2113559596","https://openalex.org/W2158894328","https://openalex.org/W2166642834","https://openalex.org/W2398032874","https://openalex.org/W2417428323","https://openalex.org/W2501460430","https://openalex.org/W2546914187","https://openalex.org/W2578914510","https://openalex.org/W2732264451","https://openalex.org/W2741396292","https://openalex.org/W2782513028","https://openalex.org/W2789695849","https://openalex.org/W2793805656","https://openalex.org/W2887875179","https://openalex.org/W2918013093","https://openalex.org/W3025280172","https://openalex.org/W3037555113","https://openalex.org/W3081439508","https://openalex.org/W3096380720","https://openalex.org/W3126299183","https://openalex.org/W3128049022","https://openalex.org/W3134004139","https://openalex.org/W3205440159","https://openalex.org/W3209790217","https://openalex.org/W4205666603","https://openalex.org/W4205804701","https://openalex.org/W4220755653"],"related_works":["https://openalex.org/W3205440159","https://openalex.org/W4405207168","https://openalex.org/W4205804701","https://openalex.org/W4293564851","https://openalex.org/W7119140288","https://openalex.org/W2083506607","https://openalex.org/W4393483605","https://openalex.org/W3096380720","https://openalex.org/W4319015743","https://openalex.org/W2103435208"],"abstract_inverted_index":{"African":[0],"swine":[1,5],"fever":[2,6],"(ASF),":[3],"classical":[4],"(CSF),":[7],"and":[8,11,22,34,48,53,66,85,96,106,110,129,134,147,174,183,193,199,224,243,248,255,258,270,286,292,297,312],"porcine":[9,152,163,169,175],"reproductive":[10],"respiratory":[12],"syndrome":[13],"(PRRS)":[14],"are":[15],"highly":[16],"infectious":[17],"diseases":[18],"of":[19,27,50,68,82,112,139,141,187,190,240,267,294],"domestic":[20],"pigs":[21],"wild":[23],"boars.":[24],"The":[25,100,117,178,205,237,273],"co-infections":[26],"ASF":[28],"virus":[29,32,36,157,167],"(ASFV),":[30],"CSF":[31],"(CSFV),":[33],"PRRS":[35],"(PRRSV)":[37],"have":[38],"been":[39],"reported":[40],"in":[41,55,87,213,234],"different":[42,101,210],"pig":[43,211],"farms.":[44],"Early":[45],"differential":[46,80],"detection":[47,81,140,291],"diagnosis":[49],"ASFV,":[51,83,132,241,295,310],"CSFV,":[52,84,133,242,296,311],"PRRSV":[54,86,135,244],"the":[56,63,107,113,121,191,220,251,261,277,290,301,305],"clinical":[57,207],"samples":[58,208],"is":[59,300],"very":[60],"important":[61],"for":[62,79,289,308],"effective":[64],"prevention":[65],"control":[67],"these":[69],"diseases.":[70],"A":[71,160],"multiplex":[72,122,221,227,252,262,279,306],"crystal":[73,123,222,253,280],"digital":[74],"PCR":[75],"(dPCR)":[76],"was":[77,125,282],"developed":[78],"this":[88],"study,":[89],"targeting":[90],"p72,":[91],"5'":[92],"untranslated":[93],"region":[94],"(UTR),":[95],"ORF7":[97],"genes,":[98],"respectively.":[99,204,272],"reaction":[102],"conditions":[103],"were":[104,115,217,245],"optimized,":[105],"specificity,":[108],"sensitivity,":[109],"repeatability":[111,182],"assay":[114,179],"evaluated.":[116],"results":[118,274],"showed":[119,180],"that":[120,276],"dPCR":[124,223,281,307],"able":[126],"to":[127,197,202],"accurately":[128],"differentially":[130],"detect":[131,150],"with":[136,185,264],"a":[137,225,283],"limit":[138],"4.69":[142],"\u00d7":[143],"10\u22121":[144],"copies/\u03bcl,":[145],"respectively,":[146],"could":[148],"not":[149],"other":[151],"viruses,":[153],"i.e.,":[154],"foot-and-mouth":[155],"disease":[156],"(FMDV),":[158],"Senecavirus":[159],"(SVA),":[161],"atypical":[162],"pestivirus":[164],"(APPV),":[165],"pseudorabies":[166],"(PRV),":[168],"circovirus":[170],"type":[171],"2":[172],"(PCV2),":[173],"parvovirus":[176],"(PPV).":[177],"excellent":[181],"reproducibility,":[184],"coefficients":[186],"variation":[188],"(CV)":[189],"intra-":[192],"inter-assay":[194],"from":[195,200,209],"0.09":[196],"1.40%,":[198],"0.64":[201],"2.26%,":[203],"289":[206],"herds":[212],"Guangxi":[214],"province,":[215],"China,":[216],"tested":[218],"by":[219,250,260],"reference":[226],"real-time":[228],"quantitative":[229],"RT-PCR":[230],"(qRT-PCR)":[231],"established":[232,278],"previously":[233],"our":[235],"laboratory.":[236],"positive":[238],"rates":[239,266],"30.10,":[246],"13.49,":[247],"22.49%":[249],"dPCR,":[254],"24.57,":[256],"8.65,":[257],"18.34%":[259],"qRT-PCR,":[263],"coincidence":[265],"94.66,":[268],"95.16,":[269],"95.84%,":[271],"indicated":[275],"specific,":[284],"sensitive,":[285],"accurate":[287],"method":[288],"quantification":[293],"PRRSV.":[298,313],"This":[299],"first":[302],"report":[303],"on":[304],"detecting":[309]},"counts_by_year":[{"year":2026,"cited_by_count":7},{"year":2025,"cited_by_count":9},{"year":2024,"cited_by_count":4},{"year":2023,"cited_by_count":7},{"year":2022,"cited_by_count":1}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2022-07-13T00:00:00"}