{"id":"https://openalex.org/W4284894834","doi":"https://doi.org/10.1021/acssynbio.1c00626","title":"High-Throughput Aminoacyl-tRNA Synthetase Engineering for Genetic Code Expansion in Yeast","display_name":"High-Throughput Aminoacyl-tRNA Synthetase Engineering for Genetic Code Expansion in Yeast","publication_year":2022,"publication_date":"2022-07-06","ids":{"openalex":"https://openalex.org/W4284894834","doi":"https://doi.org/10.1021/acssynbio.1c00626","pmid":"https://pubmed.ncbi.nlm.nih.gov/35793554","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/10065163"},"language":"en","primary_location":{"id":"doi:10.1021/acssynbio.1c00626","doi":"https://doi.org/10.1021/acssynbio.1c00626","is_oa":false,"landing_page_url":"https://doi.org/10.1021/acssynbio.1c00626","pdf_url":null,"source":{"id":"https://openalex.org/S54167817","display_name":"ACS Synthetic Biology","issn_l":"2161-5063","issn":["2161-5063"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ACS Synthetic Biology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2021/12/03/2021.07.13.452272.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5059232605","display_name":"Jessica T. Stieglitz","orcid":"https://orcid.org/0000-0003-3845-0712","observed_orcids":["https://orcid.org/0000-0003-3845-0712"]},"institutions":[{"id":"https://openalex.org/I121934306","display_name":"Tufts University","ror":"https://ror.org/05wvpxv85","country_code":"US","type":"education","lineage":["https://openalex.org/I121934306"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jessica T. Stieglitz","raw_affiliation_strings":["Chemical and Biological Engineering Department, Tufts University, Medford, Massachusetts 02155, United States"],"raw_orcid":"https://orcid.org/0000-0003-3845-0712","affiliations":[{"raw_affiliation_string":"Chemical and Biological Engineering Department, Tufts University, Medford, Massachusetts 02155, United States","institution_ids":["https://openalex.org/I121934306"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5062531357","display_name":"James A. Van Deventer","orcid":"https://orcid.org/0000-0003-4343-6157","observed_orcids":["https://orcid.org/0000-0003-4343-6157"]},"institutions":[{"id":"https://openalex.org/I121934306","display_name":"Tufts University","ror":"https://ror.org/05wvpxv85","country_code":"US","type":"education","lineage":["https://openalex.org/I121934306"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"James A. Van Deventer","raw_affiliation_strings":["Biomedical Engineering Department, Tufts University, Medford, Massachusetts 02155, United States","Chemical and Biological Engineering Department, Tufts University, Medford, Massachusetts 02155, United States"],"raw_orcid":"https://orcid.org/0000-0003-4343-6157","affiliations":[{"raw_affiliation_string":"Biomedical Engineering Department, Tufts University, Medford, Massachusetts 02155, United States","institution_ids":["https://openalex.org/I121934306"]},{"raw_affiliation_string":"Chemical and Biological Engineering Department, Tufts University, Medford, Massachusetts 02155, United States","institution_ids":["https://openalex.org/I121934306"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5062531357"],"corresponding_institution_ids":["https://openalex.org/I121934306"],"apc_list":{"value":5000,"currency":"USD","value_usd":5000},"apc_paid":null,"fwci":2.843,"has_fulltext":true,"cited_by_count":37,"citation_normalized_percentile":{"value":0.92967634,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"11","issue":"7","first_page":"2284","last_page":"2299"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10911","display_name":"Chemical Synthesis and Analysis","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11482","display_name":"RNA modifications and cancer","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/aminoacyl-trna-synthetase","display_name":"aminoacyl-tRNA synthetase","score":0.9700000286102295},{"id":"https://openalex.org/keywords/genetic-code-expansion","display_name":"genetic code expansion","score":0.9430000185966492},{"id":"https://openalex.org/keywords/orthogonal-translation-systems","display_name":"orthogonal translation systems","score":0.8479999899864197},{"id":"https://openalex.org/keywords/unnatural-amino-acids","display_name":"unnatural amino acids","score":0.9670000076293945},{"id":"https://openalex.org/keywords/yeast-surface-display","display_name":"yeast surface display","score":0.7630000114440918},{"id":"https://openalex.org/keywords/fluorescence-activated-cell-sorting","display_name":"fluorescence-activated cell sorting","score":0.9549999833106995},{"id":"https://openalex.org/keywords/high-throughput-screening","display_name":"high-throughput screening","score":0.8169999718666077},{"id":"https://openalex.org/keywords/polyspecificity","display_name":"polyspecificity","score":0.6179999709129333}],"concepts":[{"id":"https://openalex.org/C2779315393","wikidata":"https://www.wikidata.org/wiki/Q415968","display_name":"Aminoacyl tRNA synthetase","level":5,"score":0.8399336338043213},{"id":"https://openalex.org/C10853874","wikidata":"https://www.wikidata.org/wiki/Q180618","display_name":"Genetic code","level":3,"score":0.8194494247436523},{"id":"https://openalex.org/C191908910","wikidata":"https://www.wikidata.org/wiki/Q862838","display_name":"Synthetic biology","level":2,"score":0.68923020362854},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.6526929140090942},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5708246231079102},{"id":"https://openalex.org/C147816474","wikidata":"https://www.wikidata.org/wiki/Q169525","display_name":"Protein engineering","level":3,"score":0.5702953338623047},{"id":"https://openalex.org/C2910418148","wikidata":"https://www.wikidata.org/wiki/Q415968","display_name":"Amino Acyl-tRNA Synthetases","level":5,"score":0.5566650629043579},{"id":"https://openalex.org/C2779222958","wikidata":"https://www.wikidata.org/wiki/Q45422","display_name":"Yeast","level":2,"score":0.5124130845069885},{"id":"https://openalex.org/C9418097","wikidata":"https://www.wikidata.org/wiki/Q440681","display_name":"Directed evolution","level":4,"score":0.478265643119812},{"id":"https://openalex.org/C153957851","wikidata":"https://www.wikidata.org/wiki/Q201448","display_name":"Transfer RNA","level":4,"score":0.47228890657424927},{"id":"https://openalex.org/C149364088","wikidata":"https://www.wikidata.org/wiki/Q185917","display_name":"Translation (biology)","level":4,"score":0.45570266246795654},{"id":"https://openalex.org/C145290725","wikidata":"https://www.wikidata.org/wiki/Q5533489","display_name":"Genome engineering","level":5,"score":0.4555548131465912},{"id":"https://openalex.org/C51323132","wikidata":"https://www.wikidata.org/wiki/Q626251","display_name":"High-throughput screening","level":2,"score":0.44099700450897217},{"id":"https://openalex.org/C2777576037","wikidata":"https://www.wikidata.org/wiki/Q719725","display_name":"Saccharomyces cerevisiae","level":3,"score":0.43120265007019043},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.41605573892593384},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.35352712869644165},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.25992780923843384},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.25605931878089905},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.18369227647781372},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.15652459859848022},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.13806703686714172},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.12310802936553955},{"id":"https://openalex.org/C144501496","wikidata":"https://www.wikidata.org/wiki/Q5533489","display_name":"Genome editing","level":4,"score":0.1039799153804779},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.0820486843585968}],"mesh":[{"descriptor_ui":"D000596","descriptor_name":"Amino Acids","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000604","descriptor_name":"Amino Acyl-tRNA Synthetases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005815","descriptor_name":"Genetic Code","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012343","descriptor_name":"RNA, Transfer","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012343","descriptor_name":"RNA, Transfer","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012441","descriptor_name":"Saccharomyces cerevisiae","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012441","descriptor_name":"Saccharomyces cerevisiae","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D015202","descriptor_name":"Protein Engineering","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018388","descriptor_name":"Codon, Terminator","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1021/acssynbio.1c00626","doi":"https://doi.org/10.1021/acssynbio.1c00626","is_oa":false,"landing_page_url":"https://doi.org/10.1021/acssynbio.1c00626","pdf_url":null,"source":{"id":"https://openalex.org/S54167817","display_name":"ACS Synthetic Biology","issn_l":"2161-5063","issn":["2161-5063"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ACS Synthetic Biology","raw_type":"journal-article"},{"id":"doi:10.1101/2021.07.13.452272","doi":"https://doi.org/10.1101/2021.07.13.452272","is_oa":true,"landing_page_url":"https://doi.org/10.1101/2021.07.13.452272","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2021/12/03/2021.07.13.452272.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},{"id":"pmid:35793554","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/35793554","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ACS synthetic biology","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:10065163","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/10065163","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC10065163/pdf/nihms-1881738.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"ACS Synth Biol","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1101/2021.07.13.452272","doi":"https://doi.org/10.1101/2021.07.13.452272","is_oa":true,"landing_page_url":"https://doi.org/10.1101/2021.07.13.452272","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2021/12/03/2021.07.13.452272.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1059077631","display_name":null,"funder_award_id":"2016231237","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"},{"id":"https://openalex.org/G4393457492","display_name":"BD Influx 5 Laser Fluorescence Activated Cell Sorter","funder_award_id":"1s10od016196-01","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G529895842","display_name":null,"funder_award_id":"W911NF-16-1-0175","funder_id":"https://openalex.org/F4320338281","funder_display_name":"Army Research Office"},{"id":"https://openalex.org/G657448715","display_name":null,"funder_award_id":"W911NF-16-1-","funder_id":"https://openalex.org/F4320338281","funder_display_name":"Army Research Office"},{"id":"https://openalex.org/G7452299184","display_name":null,"funder_award_id":"W911NF","funder_id":"https://openalex.org/F4320338281","funder_display_name":"Army Research Office"},{"id":"https://openalex.org/G9000884125","display_name":"Leveraging the yeast surface as a platform for inhibitor discovery","funder_award_id":"R35GM133471","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320306076","display_name":"National Science Foundation","ror":"https://ror.org/021nxhr62"},{"id":"https://openalex.org/F4320310245","display_name":"Tufts University","ror":"https://ror.org/05wvpxv85"},{"id":"https://openalex.org/F4320316943","display_name":"Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology","ror":"https://ror.org/01xd6q208"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"},{"id":"https://openalex.org/F4320338281","display_name":"Army Research Office","ror":"https://ror.org/05epdh915"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4284894834.pdf","grobid_xml":"https://content.openalex.org/works/W4284894834.grobid-xml"},"referenced_works_count":80,"referenced_works":["https://openalex.org/W914903399","https://openalex.org/W1963986378","https://openalex.org/W1966287798","https://openalex.org/W1968457477","https://openalex.org/W1970652428","https://openalex.org/W1976230851","https://openalex.org/W1978858109","https://openalex.org/W1986561802","https://openalex.org/W1989255556","https://openalex.org/W1989876381","https://openalex.org/W1996310883","https://openalex.org/W2001083926","https://openalex.org/W2007639714","https://openalex.org/W2013875596","https://openalex.org/W2014611104","https://openalex.org/W2020619563","https://openalex.org/W2024156903","https://openalex.org/W2026089250","https://openalex.org/W2036078885","https://openalex.org/W2051126826","https://openalex.org/W2060391020","https://openalex.org/W2062660287","https://openalex.org/W2063542397","https://openalex.org/W2065661162","https://openalex.org/W2081416817","https://openalex.org/W2094654556","https://openalex.org/W2094688585","https://openalex.org/W2104722248","https://openalex.org/W2107167859","https://openalex.org/W2109105488","https://openalex.org/W2109155760","https://openalex.org/W2134988306","https://openalex.org/W2146913917","https://openalex.org/W2148313902","https://openalex.org/W2170374832","https://openalex.org/W2172121653","https://openalex.org/W2197946614","https://openalex.org/W2261688117","https://openalex.org/W2270783611","https://openalex.org/W2412132105","https://openalex.org/W2513614261","https://openalex.org/W2522501918","https://openalex.org/W2527322609","https://openalex.org/W2604888580","https://openalex.org/W2606822983","https://openalex.org/W2607219825","https://openalex.org/W2617704854","https://openalex.org/W2624136383","https://openalex.org/W2763278454","https://openalex.org/W2766423195","https://openalex.org/W2770490738","https://openalex.org/W2782374143","https://openalex.org/W2783185708","https://openalex.org/W2789971571","https://openalex.org/W2807170361","https://openalex.org/W2883738486","https://openalex.org/W2884127564","https://openalex.org/W2885139429","https://openalex.org/W2885764921","https://openalex.org/W2888598366","https://openalex.org/W2927651188","https://openalex.org/W2947168009","https://openalex.org/W2947928962","https://openalex.org/W2969596202","https://openalex.org/W2984786470","https://openalex.org/W2985855765","https://openalex.org/W3001997065","https://openalex.org/W3016984298","https://openalex.org/W3087176459","https://openalex.org/W3122766064","https://openalex.org/W3125287691","https://openalex.org/W3128280070","https://openalex.org/W3151309882","https://openalex.org/W3155960961","https://openalex.org/W3209704773","https://openalex.org/W4210752280","https://openalex.org/W4225364169","https://openalex.org/W4225815724","https://openalex.org/W4225963375","https://openalex.org/W4231832388"],"related_works":["https://openalex.org/W4410705054","https://openalex.org/W2604888580","https://openalex.org/W2443220578","https://openalex.org/W4392667836","https://openalex.org/W6930433521","https://openalex.org/W3084387642","https://openalex.org/W3015450180","https://openalex.org/W7165922349","https://openalex.org/W4414393401","https://openalex.org/W4381189791"],"abstract_inverted_index":{"Abstract":[0,239],"Protein":[1],"expression":[2],"with":[3,81,133,190],"genetically":[4],"encoded":[5,100],"noncanonical":[6],"amino":[7],"acids":[8],"(ncAAs)":[9],"benefits":[10],"a":[11,74,201],"broad":[12],"range":[13,202,215],"of":[14,19,32,37,88,96,105,108,117,137,141,157,170,184,203,216],"applications,":[15],"from":[16],"the":[17,30,35,106,154,179],"discovery":[18],"biological":[20,24],"therapeutics":[21],"to":[22,85,130,178,188,232],"fundamental":[23,180],"studies.":[25],"A":[26,214],"major":[27],"factor":[28],"limiting":[29],"use":[31,73],"ncAAs":[33,97],"is":[34,198],"lack":[36],"orthogonal":[38,66],"translation":[39,132],"systems":[40],"(OTSs)":[41],"that":[42],"support":[43,131],"efficient":[44],"genetic":[45,163,225],"code":[46,164,226],"expansion":[47,227],"at":[48],"repurposed":[49],"stop":[50],"codons.":[51],"Aminoacyl-tRNA":[52],"synthetases":[53],"(aaRSs)":[54],"have":[55],"been":[56],"extensively":[57],"evolved":[58],"in":[59,67,90,101,148,166,228],"E.":[60],"coli":[61],"but":[62],"are":[63],"not":[64,98],"always":[65],"eukaryotes.":[68],"In":[69],"this":[70,149],"work,":[71],"we":[72,146],"yeast":[75],"display-based":[76],"ncAA":[77,122],"incorporation":[78,95],"reporter":[79],"platform":[80],"fluorescence-activated":[82],"cell":[83],"sorting":[84],"screen":[86],"libraries":[87],"aaRSs":[89,160],"high":[91],"throughput":[92],"for":[93,162,200,224],"1)":[94],"previously":[99,142],"yeast;":[102],"2)":[103],"improvement":[104],"performance":[107],"an":[109],"existing":[110],"aaRS;":[111],"3)":[112],"highly":[113],"selective":[114],"OTSs":[115,126,189],"capable":[116],"discriminating":[118],"between":[119],"closely":[120],"related":[121,177],"analogs;":[123],"and":[124,182,193,210,235],"4)":[125],"exhibiting":[127],"enhanced":[128],"polyspecificity":[129],"structurally":[134],"diverse":[135,191,221],"sets":[136],"ncAAs.":[138],"The":[139,168],"number":[140,156],"undiscovered":[143],"aaRS":[144],"variants":[145],"report":[147],"work":[150],"more":[151],"than":[152],"doubles":[153],"total":[155],"translationally":[158],"active":[159],"available":[161],"manipulation":[165],"yeast.":[167],"success":[169],"myriad":[171],"screening":[172],"strategies":[173],"has":[174],"important":[175],"implications":[176],"properties":[181,197],"evolvability":[183],"aaRSs.":[185],"Furthermore,":[186],"access":[187,231],"activities":[192],"specific":[194],"or":[195],"polyspecific":[196],"invaluable":[199],"applications":[204],"within":[205],"chemical":[206],"biology,":[207,209],"synthetic":[208],"protein":[211],"engineering.":[212],"Synopsis":[213],"flow":[217],"cytometry-based":[218],"screens":[219],"yielded":[220],"translational":[222],"machinery":[223],"yeast,":[229],"facilitating":[230],"new":[233],"chemistries":[234],"tunable":[236],"specificity":[237],"profiles.":[238],"Figure":[240]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":11},{"year":2024,"cited_by_count":13},{"year":2023,"cited_by_count":7},{"year":2022,"cited_by_count":4},{"year":2021,"cited_by_count":1}],"updated_date":"2026-11-03T10:50:19.383937","created_date":"2022-07-09T00:00:00"}