{"id":"https://openalex.org/W4284883717","doi":"https://doi.org/10.1016/j.ymben.2022.07.002","title":"Single cell mutant selection for metabolic engineering of actinomycetes","display_name":"Single cell mutant selection for metabolic engineering of actinomycetes","publication_year":2022,"publication_date":"2022-07-06","ids":{"openalex":"https://openalex.org/W4284883717","doi":"https://doi.org/10.1016/j.ymben.2022.07.002","pmid":"https://pubmed.ncbi.nlm.nih.gov/35809806"},"language":"en","primary_location":{"id":"doi:10.1016/j.ymben.2022.07.002","doi":"https://doi.org/10.1016/j.ymben.2022.07.002","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.ymben.2022.07.002","pdf_url":"https://www.sciencedirect.com/science/article/pii/S1096717622000891/pdf","source":{"id":"https://openalex.org/S173328182","display_name":"Metabolic Engineering","issn_l":"1096-7176","issn":["1096-7176","1096-7184"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-2","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Metabolic Engineering","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://www.sciencedirect.com/science/article/pii/S1096717622000891/pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5080151502","display_name":"Amir Behzad AKHGARI","orcid":"https://orcid.org/0000-0002-1237-7425","observed_orcids":["https://orcid.org/0000-0002-1237-7425","https://orcid.org/0009-0008-3865-1761"]},"institutions":[{"id":"https://openalex.org/I155660961","display_name":"University of Turku","ror":"https://ror.org/05vghhr25","country_code":"FI","type":"education","lineage":["https://openalex.org/I155660961"]}],"countries":["FI"],"is_corresponding":false,"raw_author_name":"Amir Akhgari","raw_affiliation_strings":["Department of Life Sciences, University of Turku, Turku, FIN, 20014, Finland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Life Sciences, University of Turku, Turku, FIN, 20014, Finland","institution_ids":["https://openalex.org/I155660961"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5047088050","display_name":"Bikash Baral","orcid":"https://orcid.org/0000-0003-2085-9528","observed_orcids":["https://orcid.org/0000-0003-2085-9528"]},"institutions":[{"id":"https://openalex.org/I155660961","display_name":"University of Turku","ror":"https://ror.org/05vghhr25","country_code":"FI","type":"education","lineage":["https://openalex.org/I155660961"]}],"countries":["FI"],"is_corresponding":false,"raw_author_name":"Bikash Baral","raw_affiliation_strings":["Department of Life Sciences, University of Turku, Turku, FIN, 20014, Finland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Life Sciences, University of Turku, Turku, FIN, 20014, Finland","institution_ids":["https://openalex.org/I155660961"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5024569158","display_name":"Arina Koroleva","orcid":"https://orcid.org/0000-0002-0660-0408","observed_orcids":["https://orcid.org/0000-0002-0660-0408"]},"institutions":[{"id":"https://openalex.org/I155660961","display_name":"University of Turku","ror":"https://ror.org/05vghhr25","country_code":"FI","type":"education","lineage":["https://openalex.org/I155660961"]}],"countries":["FI"],"is_corresponding":false,"raw_author_name":"Arina Koroleva","raw_affiliation_strings":["Department of Life Sciences, University of Turku, Turku, FIN, 20014, Finland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Life Sciences, University of Turku, Turku, FIN, 20014, Finland","institution_ids":["https://openalex.org/I155660961"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5038994232","display_name":"Vilja Siitonen","orcid":"https://orcid.org/0000-0002-1444-2622","observed_orcids":["https://orcid.org/0000-0002-1444-2622"]},"institutions":[{"id":"https://openalex.org/I155660961","display_name":"University of Turku","ror":"https://ror.org/05vghhr25","country_code":"FI","type":"education","lineage":["https://openalex.org/I155660961"]}],"countries":["FI"],"is_corresponding":false,"raw_author_name":"Vilja Siitonen","raw_affiliation_strings":["Department of Life Sciences, University of Turku, Turku, FIN, 20014, Finland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Life Sciences, University of Turku, Turku, FIN, 20014, Finland","institution_ids":["https://openalex.org/I155660961"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5038347874","display_name":"David Peter Fewer","orcid":"https://orcid.org/0000-0003-3978-4845","observed_orcids":["https://orcid.org/0000-0003-3978-4845"]},"institutions":[{"id":"https://openalex.org/I133731052","display_name":"University of Helsinki","ror":"https://ror.org/040af2s02","country_code":"FI","type":"education","lineage":["https://openalex.org/I133731052"]}],"countries":["FI"],"is_corresponding":false,"raw_author_name":"David P. Fewer","raw_affiliation_strings":["Department of Microbiology, University of Helsinki, Helsinki, FIN, 00014, Finland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, University of Helsinki, Helsinki, FIN, 00014, Finland","institution_ids":["https://openalex.org/I133731052"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5051788322","display_name":"Charles E. Melan\u00e7on","orcid":"https://orcid.org/0000-0002-4236-3868","observed_orcids":["https://orcid.org/0000-0002-4236-3868"]},"institutions":[{"id":"https://openalex.org/I169521973","display_name":"University of New Mexico","ror":"https://ror.org/05fs6jp91","country_code":"US","type":"education","lineage":["https://openalex.org/I169521973"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Charles E. Melan\u00e7on","raw_affiliation_strings":["Department of Chemistry and Chemical Biology, University of New Mexico, Albuquerque, NM, 87131-0001, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Chemical Biology, University of New Mexico, Albuquerque, NM, 87131-0001, USA","institution_ids":["https://openalex.org/I169521973"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5032129171","display_name":"Jani Rahkila","orcid":"https://orcid.org/0000-0002-5491-0574","observed_orcids":["https://orcid.org/0000-0002-5491-0574"]},"institutions":[{"id":"https://openalex.org/I130217899","display_name":"\u00c5bo Akademi University","ror":"https://ror.org/029pk6x14","country_code":"FI","type":"education","lineage":["https://openalex.org/I130217899"]}],"countries":["FI"],"is_corresponding":false,"raw_author_name":"Jani Rahkila","raw_affiliation_strings":["Instrument Centre, Faculty of Science and Engineering, \u00c5bo Akademi University, Turku, FIN, 20500, Finland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Instrument Centre, Faculty of Science and Engineering, \u00c5bo Akademi University, Turku, FIN, 20500, Finland","institution_ids":["https://openalex.org/I130217899"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5003353973","display_name":"Mikko Mets\u00e4\u2010Ketel\u00e4","orcid":"https://orcid.org/0000-0003-3176-2908","observed_orcids":["https://orcid.org/0000-0003-3176-2908"]},"institutions":[{"id":"https://openalex.org/I155660961","display_name":"University of Turku","ror":"https://ror.org/05vghhr25","country_code":"FI","type":"education","lineage":["https://openalex.org/I155660961"]}],"countries":["FI"],"is_corresponding":true,"raw_author_name":"Mikko Mets\u00e4-Ketel\u00e4","raw_affiliation_strings":["Department of Life Sciences, University of Turku, Turku, FIN, 20014, Finland. Electronic address: mianme@utu.fi","Department of Life Sciences, University of Turku, Turku, FIN, 20014, Finland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Life Sciences, University of Turku, Turku, FIN, 20014, Finland. Electronic address: mianme@utu.fi","institution_ids":["https://openalex.org/I155660961"]},{"raw_affiliation_string":"Department of Life Sciences, University of Turku, Turku, FIN, 20014, Finland","institution_ids":["https://openalex.org/I155660961"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5003353973"],"corresponding_institution_ids":["https://openalex.org/I155660961"],"apc_list":{"value":4870,"currency":"USD","value_usd":4870},"apc_paid":{"value":4870,"currency":"USD","value_usd":4870},"fwci":0.9788,"has_fulltext":true,"cited_by_count":17,"citation_normalized_percentile":{"value":0.71755389,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":94,"max":99},"biblio":{"volume":"73","issue":null,"first_page":"124","last_page":"133"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.5562000274658203,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.5562000274658203,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10252","display_name":"Microbial Natural Products and Biosynthesis","score":0.3865000009536743,"subfield":{"id":"https://openalex.org/subfields/2736","display_name":"Pharmacology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.01590000092983246,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/actinomycetes","display_name":"actinomycetes","score":0.9559999704360962},{"id":"https://openalex.org/keywords/fluorescence-activated-cell-sorting","display_name":"fluorescence-activated cell sorting","score":0.8679999709129333},{"id":"https://openalex.org/keywords/random-mutagenesis","display_name":"random mutagenesis","score":0.8420000076293945},{"id":"https://openalex.org/keywords/cholesterol-oxidase","display_name":"cholesterol oxidase","score":0.675000011920929},{"id":"https://openalex.org/keywords/streptomyces-lavendulae","display_name":"Streptomyces lavendulae","score":0.9929999709129333},{"id":"https://openalex.org/keywords/amycolatopsis","display_name":"Amycolatopsis","score":0.9890000224113464},{"id":"https://openalex.org/keywords/genome-mining","display_name":"genome mining","score":0.6679999828338623},{"id":"https://openalex.org/keywords/high-throughput-screening","display_name":"high-throughput screening","score":0.6299999952316284}],"concepts":[{"id":"https://openalex.org/C6350086","wikidata":"https://www.wikidata.org/wiki/Q6822347","display_name":"Metabolic engineering","level":3,"score":0.849345862865448},{"id":"https://openalex.org/C9418097","wikidata":"https://www.wikidata.org/wiki/Q440681","display_name":"Directed evolution","level":4,"score":0.6664887070655823},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.6285392045974731},{"id":"https://openalex.org/C2778630268","wikidata":"https://www.wikidata.org/wiki/Q17113277","display_name":"Cell sorting","level":3,"score":0.5889080762863159},{"id":"https://openalex.org/C192989942","wikidata":"https://www.wikidata.org/wiki/Q68685","display_name":"Metabolic pathway","level":3,"score":0.5496153831481934},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5305640697479248},{"id":"https://openalex.org/C51323132","wikidata":"https://www.wikidata.org/wiki/Q626251","display_name":"High-throughput screening","level":2,"score":0.4961884617805481},{"id":"https://openalex.org/C2908647359","wikidata":"https://www.wikidata.org/wiki/Q2625603","display_name":"Population","level":2,"score":0.48401686549186707},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.47358518838882446},{"id":"https://openalex.org/C16318435","wikidata":"https://www.wikidata.org/wiki/Q149299","display_name":"Mutagenesis","level":4,"score":0.4641704559326172},{"id":"https://openalex.org/C191908910","wikidata":"https://www.wikidata.org/wiki/Q862838","display_name":"Synthetic biology","level":2,"score":0.46125543117523193},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.45035916566848755},{"id":"https://openalex.org/C147816474","wikidata":"https://www.wikidata.org/wiki/Q169525","display_name":"Protein engineering","level":3,"score":0.41258472204208374},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.35592225193977356},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.2415926456451416},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.139163076877594},{"id":"https://openalex.org/C144024400","wikidata":"https://www.wikidata.org/wiki/Q21201","display_name":"Sociology","level":0,"score":0.0},{"id":"https://openalex.org/C149923435","wikidata":"https://www.wikidata.org/wiki/Q37732","display_name":"Demography","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000190","descriptor_name":"Actinomyces","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016296","descriptor_name":"Mutagenesis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D039903","descriptor_name":"Actinobacteria","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D053858","descriptor_name":"Metabolic Networks and Pathways","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D060847","descriptor_name":"Metabolic Engineering","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":7,"locations":[{"id":"doi:10.1016/j.ymben.2022.07.002","doi":"https://doi.org/10.1016/j.ymben.2022.07.002","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.ymben.2022.07.002","pdf_url":"https://www.sciencedirect.com/science/article/pii/S1096717622000891/pdf","source":{"id":"https://openalex.org/S173328182","display_name":"Metabolic Engineering","issn_l":"1096-7176","issn":["1096-7176","1096-7184"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-2","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Metabolic Engineering","raw_type":"journal-article"},{"id":"doi:10.1101/2022.01.13.476137","doi":"https://doi.org/10.1101/2022.01.13.476137","is_oa":true,"landing_page_url":"https://doi.org/10.1101/2022.01.13.476137","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2022/01/14/2022.01.13.476137.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},{"id":"pmid:35809806","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/35809806","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Metabolic engineering","raw_type":"Journal Article"},{"id":"pmh:oai:utupub.fi:11111/35743","doi":null,"is_oa":true,"landing_page_url":"https://www.utupub.fi/handle/11111/35743","pdf_url":"https://www.utupub.fi/bitstreams/aee227c1-41ca-46a9-99be-b51548e339ff/download","source":{"id":"https://openalex.org/S4306402470","display_name":"UTUPub (University of Turku)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I155660961","host_organization_name":"University of Turku","host_organization_lineage":["https://openalex.org/I155660961"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"https://www.utupub.fi/handle/10024/161367","raw_type":null},{"id":"pmh:oai:www.utupub.fi:10024/161367","doi":null,"is_oa":true,"landing_page_url":"https://www.utupub.fi/handle/10024/161367","pdf_url":"https://www.utupub.fi/bitstream/10024/161367/1/1-s2.0-S1096717622000891-main.pdf","source":{"id":"https://openalex.org/S4306402470","display_name":"UTUPub (University of Turku)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I155660961","host_organization_name":"University of Turku","host_organization_lineage":["https://openalex.org/I155660961"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null},{"id":"pmh:oai:helda.helsinki.fi:10138/349858","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10138/349858","pdf_url":null,"source":{"id":"https://openalex.org/S4210213322","display_name":"Ty\u00f6v\u00e4entutkimus Vuosikirja","issn_l":"0784-1272","issn":["0784-1272","1459-7780"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":["ddh","jufo-1"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Article"},{"id":"pmh:oai:pure.atira.dk:openaire/a2ec22cb-c038-4bb8-958a-79114bc1b358","doi":null,"is_oa":true,"landing_page_url":"https://research.abo.fi/en/publications/a2ec22cb-c038-4bb8-958a-79114bc1b358","pdf_url":null,"source":{"id":"https://openalex.org/S4406923060","display_name":"\u00c5bo Akademi University Research Portal","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Akhgari, A, Baral, B, Koroleva, A, Siitonen, V, Fewer, D P, Melan\u00e7on, C E, Rahkila, J & Mets\u00e4-Ketel\u00e4, M 2022, 'Single cell mutant selection for metabolic engineering of actinomycetes', Metabolic Engineering, vol. 73, pp. 124-133. https://doi.org/10.1016/j.ymben.2022.07.002","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":{"id":"doi:10.1016/j.ymben.2022.07.002","doi":"https://doi.org/10.1016/j.ymben.2022.07.002","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.ymben.2022.07.002","pdf_url":"https://www.sciencedirect.com/science/article/pii/S1096717622000891/pdf","source":{"id":"https://openalex.org/S173328182","display_name":"Metabolic Engineering","issn_l":"1096-7176","issn":["1096-7176","1096-7184"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-2","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Metabolic Engineering","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G3509566489","display_name":null,"funder_award_id":"NNF21OC0068849","funder_id":"https://openalex.org/F4320325957","funder_display_name":"Novo Nordisk Fonden"}],"funders":[{"id":"https://openalex.org/F4320310086","display_name":"Helsingin Yliopisto","ror":"https://ror.org/040af2s02"},{"id":"https://openalex.org/F4320321726","display_name":"Suomen Kulttuurirahasto","ror":"https://ror.org/027xav248"},{"id":"https://openalex.org/F4320322282","display_name":"Jane ja Aatos Erkon S\u00e4\u00e4ti\u00f6","ror":"https://ror.org/03vxy9y38"},{"id":"https://openalex.org/F4320322436","display_name":"Novo Nordisk","ror":"https://ror.org/0435rc536"},{"id":"https://openalex.org/F4320323313","display_name":"Turun Yliopisto","ror":"https://ror.org/05vghhr25"},{"id":"https://openalex.org/F4320325957","display_name":"Novo Nordisk Fonden","ror":"https://ror.org/04txyc737"},{"id":"https://openalex.org/F4320331826","display_name":"Turun Yliopistos\u00e4\u00e4ti\u00f6","ror":"https://ror.org/02kjggt26"},{"id":"https://openalex.org/F4320333873","display_name":"Helsinki Institute of Life Science, Helsingin Yliopisto","ror":"https://ror.org/018bh0m68"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4284883717.pdf","grobid_xml":"https://content.openalex.org/works/W4284883717.grobid-xml"},"referenced_works_count":71,"referenced_works":["https://openalex.org/W29938334","https://openalex.org/W647091450","https://openalex.org/W1202680985","https://openalex.org/W1577451703","https://openalex.org/W1585422457","https://openalex.org/W1827848435","https://openalex.org/W1979197349","https://openalex.org/W1990776258","https://openalex.org/W1991265839","https://openalex.org/W1995995599","https://openalex.org/W2005424731","https://openalex.org/W2014197955","https://openalex.org/W2014470740","https://openalex.org/W2015421418","https://openalex.org/W2030728458","https://openalex.org/W2033496632","https://openalex.org/W2035133853","https://openalex.org/W2038070596","https://openalex.org/W2042325710","https://openalex.org/W2043585725","https://openalex.org/W2044965911","https://openalex.org/W2047014438","https://openalex.org/W2049611266","https://openalex.org/W2057969236","https://openalex.org/W2077999011","https://openalex.org/W2086223988","https://openalex.org/W2088562711","https://openalex.org/W2092263110","https://openalex.org/W2095063801","https://openalex.org/W2095381145","https://openalex.org/W2113033658","https://openalex.org/W2115437893","https://openalex.org/W2119030585","https://openalex.org/W2149264689","https://openalex.org/W2155967620","https://openalex.org/W2187341651","https://openalex.org/W2223717821","https://openalex.org/W2256742438","https://openalex.org/W2266839951","https://openalex.org/W2531630905","https://openalex.org/W2564833305","https://openalex.org/W2568577966","https://openalex.org/W2587440936","https://openalex.org/W2607417244","https://openalex.org/W2614151742","https://openalex.org/W2745396386","https://openalex.org/W2769732430","https://openalex.org/W2780809658","https://openalex.org/W2781691934","https://openalex.org/W2794714279","https://openalex.org/W2800847252","https://openalex.org/W2804007336","https://openalex.org/W2811415640","https://openalex.org/W2889677909","https://openalex.org/W2898639687","https://openalex.org/W2920877977","https://openalex.org/W2926049480","https://openalex.org/W2967935646","https://openalex.org/W2989693255","https://openalex.org/W3011366455","https://openalex.org/W3015304157","https://openalex.org/W3048699272","https://openalex.org/W3065125921","https://openalex.org/W3091905208","https://openalex.org/W3102931476","https://openalex.org/W3120392574","https://openalex.org/W3141351532","https://openalex.org/W3165511231","https://openalex.org/W3173596632","https://openalex.org/W3194188670","https://openalex.org/W4233143487"],"related_works":["https://openalex.org/W7048793202","https://openalex.org/W4226495135","https://openalex.org/W2089804615","https://openalex.org/W2077999011","https://openalex.org/W7220781738","https://openalex.org/W4280517077","https://openalex.org/W2491443887","https://openalex.org/W2922422116","https://openalex.org/W2972516821","https://openalex.org/W143896580"],"abstract_inverted_index":{"Abstract":[0],"Actinomycetes":[1,131],"are":[2,132],"important":[3,134],"producers":[4],"of":[5,48,70,128,136,222,224,237,241,249],"pharmaceuticals":[6],"and":[7,37,114,198,245,251],"industrial":[8,19,111,143,152],"enzymes.":[9],"However,":[10],"wild":[11],"type":[12],"strains":[13,205],"require":[14,184],"laborious":[15],"development":[16,113,168],"prior":[17],"to":[18,119,159,186,194,219,246],"usage.":[20],"Here":[21],"we":[22,199],"present":[23],"a":[24,55,67,98,125,178,227],"generally":[25],"applicable":[26],"reporter-guided":[27],"metabolic":[28,81,101,235,243],"engineering":[29,236],"tool":[30],"based":[31,63],"on":[32],"random":[33],"mutagenesis,":[34],"selective":[35],"pressure,":[36],"single-cell":[38],"sorting.":[39],"We":[40,89,106,145],"developed":[41],"fluorescence-activated":[42],"cell":[43,229],"sorting":[44],"(FACS)":[45],"methodology":[46],"capable":[47],"reproducibly":[49],"identifying":[50],"high-performing":[51],"individual":[52],"cells":[53],"from":[54,59,207],"mutant":[56,129,208],"population":[57],"directly":[58,206],"liquid":[60],"cultures.":[61],"Genome-mining":[62],"drug":[64,94],"discovery":[65,95],"is":[66,74,169,177],"promising":[68],"source":[69,135,187],"bioactive":[71],"compounds,":[72],"which":[73,176],"complicated":[75],"by":[76,96,172],"the":[77,108,217],"observation":[78],"that":[79,182],"target":[80],"pathways":[82,244],"may":[83,183],"be":[84],"silent":[85,99,242],"under":[86,211],"laboratory":[87],"conditions.":[88,214],"demonstrate":[90,146],"our":[91],"technology":[92],"for":[93,110,234,239],"activating":[97],"mutaxanthene":[100,116],"pathway":[102],"in":[103,124,164,226],"Amycolatopsis":[104],".":[105],"apply":[107],"method":[109],"strain":[112],"increase":[115,247],"yields":[117,141,248],"9-fold":[118],"99":[120],"mg":[121],"l":[122],"\u22121":[123,163],"second":[126],"round":[127],"selection.":[130],"an":[133,151],"catabolic":[137],"enzymes,":[138],"where":[139],"product":[140],"determine":[142],"viability.":[144],"5-fold":[147],"yield":[148,203],"improvement":[149],"with":[150],"cholesterol":[153],"oxidase":[154],"ChoD":[155,202],"producer":[156],"Streptomyces":[157],"lavendulae":[158],"20.4":[160],"U":[161],"g":[162],"three":[165],"rounds.":[166],"Strain":[167],"traditionally":[170],"followed":[171],"production":[173,204],"medium":[174,196],"optimization,":[175],"time-consuming":[179],"multi-parameter":[180],"problem":[181],"hard":[185],"ingredients.":[188],"Ultra-high":[189],"throughput":[190],"screening":[191],"allowed":[192],"us":[193],"circumvent":[195],"optimization":[197],"identified":[200],"high":[201],"libraries":[209],"grown":[210],"preset":[212],"culture":[213],"In":[215],"summary,":[216],"ability":[218],"screen":[220],"tens":[221],"millions":[223],"mutants":[225],"single":[228],"format":[230],"offers":[231],"broad":[232],"applicability":[233],"actinomycetes":[238],"activation":[240],"proteins":[250],"natural":[252],"products.":[253]},"counts_by_year":[{"year":2026,"cited_by_count":5},{"year":2025,"cited_by_count":4},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":6}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2022-07-09T00:00:00"}
