{"id":"https://openalex.org/W4283787899","doi":"https://doi.org/10.1101/2022.07.02.498544","title":"Optimizing multifunctional fluorescent ligands for intracellular labeling","display_name":"Optimizing multifunctional fluorescent ligands for intracellular labeling","publication_year":2022,"publication_date":"2022-07-03","ids":{"openalex":"https://openalex.org/W4283787899","doi":"https://doi.org/10.1101/2022.07.02.498544"},"language":"en","primary_location":{"id":"doi:10.1101/2022.07.02.498544","doi":"https://doi.org/10.1101/2022.07.02.498544","is_oa":true,"landing_page_url":"https://doi.org/10.1101/2022.07.02.498544","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2022/07/03/2022.07.02.498544.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nd","license_id":"https://openalex.org/licenses/cc-by-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2022/07/03/2022.07.02.498544.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5057165567","display_name":"Pratik Kumar","orcid":"https://orcid.org/0000-0002-9516-0212","observed_orcids":["https://orcid.org/0000-0002-9516-0212"]},"institutions":[{"id":"https://openalex.org/I1344073410","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410"]},{"id":"https://openalex.org/I195573530","display_name":"Janelia Research Campus","ror":"https://ror.org/013sk6x84","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410","https://openalex.org/I195573530"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Pratik Kumar","raw_affiliation_strings":["Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA"],"raw_orcid":"https://orcid.org/0000-0002-9516-0212","affiliations":[{"raw_affiliation_string":"Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA","institution_ids":["https://openalex.org/I1344073410","https://openalex.org/I195573530"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5064660866","display_name":"Jason Vevea","orcid":"https://orcid.org/0000-0002-3068-973X","observed_orcids":["https://orcid.org/0000-0002-3068-973X"]},"institutions":[{"id":"https://openalex.org/I1344073410","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410"]},{"id":"https://openalex.org/I135310074","display_name":"University of Wisconsin\u2013Madison","ror":"https://ror.org/01y2jtd41","country_code":"US","type":"education","lineage":["https://openalex.org/I135310074"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jason D. Vevea","raw_affiliation_strings":["Department of Neuroscience, University of Wisconsin\u2013Madison, Madison, WI, USA","Howard Hughes Medical Institute, University of Wisconsin\u2013Madison, Madison, WI, USA"],"raw_orcid":"https://orcid.org/0000-0002-3068-973X","affiliations":[{"raw_affiliation_string":"Department of Neuroscience, University of Wisconsin\u2013Madison, Madison, WI, USA","institution_ids":["https://openalex.org/I135310074"]},{"raw_affiliation_string":"Howard Hughes Medical Institute, University of Wisconsin\u2013Madison, Madison, WI, USA","institution_ids":["https://openalex.org/I1344073410","https://openalex.org/I135310074"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5045181969","display_name":"Ariana N. Tkachuk","orcid":"https://orcid.org/0000-0001-8991-9643","observed_orcids":["https://orcid.org/0000-0001-8991-9643"]},"institutions":[{"id":"https://openalex.org/I1344073410","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410"]},{"id":"https://openalex.org/I135310074","display_name":"University of Wisconsin\u2013Madison","ror":"https://ror.org/01y2jtd41","country_code":"US","type":"education","lineage":["https://openalex.org/I135310074"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ariana N. Tkachuk","raw_affiliation_strings":["Department of Neuroscience, University of Wisconsin\u2013Madison, Madison, WI, USA","Howard Hughes Medical Institute, University of Wisconsin\u2013Madison, Madison, WI, USA"],"raw_orcid":"https://orcid.org/0000-0001-8991-9643","affiliations":[{"raw_affiliation_string":"Department of Neuroscience, University of Wisconsin\u2013Madison, Madison, WI, USA","institution_ids":["https://openalex.org/I135310074"]},{"raw_affiliation_string":"Howard Hughes Medical Institute, University of Wisconsin\u2013Madison, Madison, WI, USA","institution_ids":["https://openalex.org/I1344073410","https://openalex.org/I135310074"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5058235802","display_name":"Kirby R. Campbell","orcid":"https://orcid.org/0000-0003-0091-4139","observed_orcids":["https://orcid.org/0000-0003-0091-4139"]},"institutions":[{"id":"https://openalex.org/I1344073410","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410"]},{"id":"https://openalex.org/I195573530","display_name":"Janelia Research Campus","ror":"https://ror.org/013sk6x84","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410","https://openalex.org/I195573530"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kirby Campbell","raw_affiliation_strings":["Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA"],"raw_orcid":"https://orcid.org/0000-0003-0091-4139","affiliations":[{"raw_affiliation_string":"Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA","institution_ids":["https://openalex.org/I1344073410","https://openalex.org/I195573530"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5041575772","display_name":"Emma T. Watson","orcid":"https://orcid.org/0000-0002-5336-8170","observed_orcids":["https://orcid.org/0000-0002-5336-8170"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Emma T. Watson","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-5336-8170","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5003357207","display_name":"Anthony X. Ayala","orcid":"https://orcid.org/0000-0002-3913-6453","observed_orcids":["https://orcid.org/0000-0002-3913-6453"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Anthony X. Ayala","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-3913-6453","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5052613436","display_name":"Jonathan B. Grimm","orcid":"https://orcid.org/0000-0003-0331-4200","observed_orcids":["https://orcid.org/0000-0003-0331-4200"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Jonathan B. Grimm","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0003-0331-4200","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5075074631","display_name":"Edwin R. Chapman","orcid":"https://orcid.org/0000-0001-9787-8140","observed_orcids":["https://orcid.org/0000-0001-9787-8140"]},"institutions":[{"id":"https://openalex.org/I1344073410","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410"]},{"id":"https://openalex.org/I135310074","display_name":"University of Wisconsin\u2013Madison","ror":"https://ror.org/01y2jtd41","country_code":"US","type":"education","lineage":["https://openalex.org/I135310074"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Edwin R. Chapman","raw_affiliation_strings":["Department of Neuroscience, University of Wisconsin\u2013Madison, Madison, WI, USA","Howard Hughes Medical Institute, University of Wisconsin\u2013Madison, Madison, WI, USA"],"raw_orcid":"https://orcid.org/0000-0001-9787-8140","affiliations":[{"raw_affiliation_string":"Department of Neuroscience, University of Wisconsin\u2013Madison, Madison, WI, USA","institution_ids":["https://openalex.org/I135310074"]},{"raw_affiliation_string":"Howard Hughes Medical Institute, University of Wisconsin\u2013Madison, Madison, WI, USA","institution_ids":["https://openalex.org/I1344073410","https://openalex.org/I135310074"]}]},{"author_position":"middle","author":{"id":null,"display_name":"David J. Solecki","orcid":"https://orcid.org/0000-0001-8481-0403","observed_orcids":["https://orcid.org/0000-0001-8481-0403"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"David J. Solecki","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0001-8481-0403","affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5042688784","display_name":"Luke D. Lavis","orcid":"https://orcid.org/0000-0002-0789-6343","observed_orcids":["https://orcid.org/0000-0002-0789-6343"]},"institutions":[{"id":"https://openalex.org/I1344073410","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410"]},{"id":"https://openalex.org/I195573530","display_name":"Janelia Research Campus","ror":"https://ror.org/013sk6x84","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410","https://openalex.org/I195573530"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Luke D. Lavis","raw_affiliation_strings":["Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA"],"raw_orcid":"https://orcid.org/0000-0002-0789-6343","affiliations":[{"raw_affiliation_string":"Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA","institution_ids":["https://openalex.org/I1344073410","https://openalex.org/I195573530"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5042688784"],"corresponding_institution_ids":["https://openalex.org/I1344073410","https://openalex.org/I195573530"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":2,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11440","display_name":"Click Chemistry and Applications","score":0.7196999788284302,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11440","display_name":"Click Chemistry and Applications","score":0.7196999788284302,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T13326","display_name":"Biochemical and Structural Characterization","score":0.0697999969124794,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13127","display_name":"Biotin and Related Studies","score":0.04479999840259552,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/halotag","display_name":"HaloTag","score":0.7210000157356262},{"id":"https://openalex.org/keywords/membrane-permeability","display_name":"membrane permeability","score":0.7609999775886536},{"id":"https://openalex.org/keywords/distribution-coefficient","display_name":"distribution coefficient","score":0.7770000100135803},{"id":"https://openalex.org/keywords/heterochromatin","display_name":"heterochromatin","score":0.9430000185966492},{"id":"https://openalex.org/keywords/hp1alpha","display_name":"HP1\u03b1","score":0.7360000014305115},{"id":"https://openalex.org/keywords/jq1","display_name":"JQ1","score":0.7799999713897705},{"id":"https://openalex.org/keywords/biotin","display_name":"biotin","score":0.5099999904632568}],"concepts":[{"id":"https://openalex.org/C2778617687","wikidata":"https://www.wikidata.org/wiki/Q2448456","display_name":"Fluorophore","level":3,"score":0.8934465646743774},{"id":"https://openalex.org/C2779916640","wikidata":"https://www.wikidata.org/wiki/Q420410","display_name":"Rhodamine","level":3,"score":0.6252360939979553},{"id":"https://openalex.org/C2778204606","wikidata":"https://www.wikidata.org/wiki/Q181354","display_name":"Biotin","level":2,"score":0.5800656080245972},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.5751073360443115},{"id":"https://openalex.org/C116569031","wikidata":"https://www.wikidata.org/wiki/Q899107","display_name":"Ligand (biochemistry)","level":3,"score":0.5418956279754639},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5273051261901855},{"id":"https://openalex.org/C169274487","wikidata":"https://www.wikidata.org/wiki/Q840211","display_name":"Fluorescence microscope","level":3,"score":0.5215585231781006},{"id":"https://openalex.org/C2994297994","wikidata":"https://www.wikidata.org/wiki/Q866317","display_name":"Cell permeability","level":2,"score":0.49531665444374084},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.4854453206062317},{"id":"https://openalex.org/C176406525","wikidata":"https://www.wikidata.org/wiki/Q24775166","display_name":"Avidin","level":3,"score":0.47857406735420227},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.4615744352340698},{"id":"https://openalex.org/C15526883","wikidata":"https://www.wikidata.org/wiki/Q6657126","display_name":"Live cell imaging","level":3,"score":0.4325235188007355},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.3521795868873596},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.25174829363822937},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.24842217564582825},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.15698307752609253},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1101/2022.07.02.498544","doi":"https://doi.org/10.1101/2022.07.02.498544","is_oa":true,"landing_page_url":"https://doi.org/10.1101/2022.07.02.498544","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2022/07/03/2022.07.02.498544.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nd","license_id":"https://openalex.org/licenses/cc-by-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.1101/2022.07.02.498544","doi":"https://doi.org/10.1101/2022.07.02.498544","is_oa":true,"landing_page_url":"https://doi.org/10.1101/2022.07.02.498544","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2022/07/03/2022.07.02.498544.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nd","license_id":"https://openalex.org/licenses/cc-by-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G6478566839","display_name":"Mechanisms regulating mitochondrial polarity in neurons: regional distribution and specialization","funder_award_id":"F32NS098604","funder_id":"https://openalex.org/F4320337359","funder_display_name":"National Institute of Neurological Disorders and Stroke"},{"id":"https://openalex.org/G8190400116","display_name":"Structure and dynamics of exocytotic fusion pores","funder_award_id":"R35NS097362","funder_id":"https://openalex.org/F4320337359","funder_display_name":"National Institute of Neurological Disorders and Stroke"},{"id":"https://openalex.org/G9000909930","display_name":"Synaptotagmin C2B Domain as a Ca2+ Sensing Module","funder_award_id":"R01MH061876","funder_id":"https://openalex.org/F4320337346","funder_display_name":"National Institute of Mental Health"}],"funders":[{"id":"https://openalex.org/F4320306082","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337346","display_name":"National Institute of Mental Health","ror":"https://ror.org/04xeg9z08"},{"id":"https://openalex.org/F4320337359","display_name":"National Institute of Neurological Disorders and Stroke","ror":"https://ror.org/01s5ya894"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4283787899.pdf","grobid_xml":"https://content.openalex.org/works/W4283787899.grobid-xml"},"referenced_works_count":66,"referenced_works":["https://openalex.org/W765815668","https://openalex.org/W1974945118","https://openalex.org/W1976672416","https://openalex.org/W1981037064","https://openalex.org/W1988774285","https://openalex.org/W1997099073","https://openalex.org/W2011697808","https://openalex.org/W2016036293","https://openalex.org/W2017455861","https://openalex.org/W2052692973","https://openalex.org/W2057141116","https://openalex.org/W2087508628","https://openalex.org/W2097559461","https://openalex.org/W2129788878","https://openalex.org/W2131543691","https://openalex.org/W2134178604","https://openalex.org/W2148103576","https://openalex.org/W2150881786","https://openalex.org/W2152024452","https://openalex.org/W2158821132","https://openalex.org/W2163128710","https://openalex.org/W2172791605","https://openalex.org/W2279872789","https://openalex.org/W2292719107","https://openalex.org/W2315391878","https://openalex.org/W2320931493","https://openalex.org/W2397093333","https://openalex.org/W2511142927","https://openalex.org/W2529007059","https://openalex.org/W2549455364","https://openalex.org/W2605776475","https://openalex.org/W2624558925","https://openalex.org/W2735247561","https://openalex.org/W2742811550","https://openalex.org/W2763278454","https://openalex.org/W2775795482","https://openalex.org/W2804701327","https://openalex.org/W2805522187","https://openalex.org/W2809057270","https://openalex.org/W2885038309","https://openalex.org/W2888368935","https://openalex.org/W2904433239","https://openalex.org/W2964363746","https://openalex.org/W2969331672","https://openalex.org/W2971618415","https://openalex.org/W2989822266","https://openalex.org/W2995091889","https://openalex.org/W2995673532","https://openalex.org/W3020215747","https://openalex.org/W3033812751","https://openalex.org/W3036358892","https://openalex.org/W3044450512","https://openalex.org/W3146748761","https://openalex.org/W3158765559","https://openalex.org/W3191245720","https://openalex.org/W3197096057","https://openalex.org/W4211115069","https://openalex.org/W4214693790","https://openalex.org/W4226005222","https://openalex.org/W4280595328","https://openalex.org/W4286373378","https://openalex.org/W4298087697","https://openalex.org/W4307715801","https://openalex.org/W4313453503","https://openalex.org/W4320480622","https://openalex.org/W4392027991"],"related_works":["https://openalex.org/W4403245781","https://openalex.org/W4412128768","https://openalex.org/W7117531292","https://openalex.org/W7133319481","https://openalex.org/W4200099080","https://openalex.org/W2754413319","https://openalex.org/W2231569015","https://openalex.org/W4388102014","https://openalex.org/W4391608107","https://openalex.org/W4405241077"],"abstract_inverted_index":{"Abstract":[0],"Enzyme-based":[1],"self-labeling":[2],"tags":[3,30],"enable":[4,195,258],"covalent":[5],"attachment":[6],"of":[7,17,107,173,200,209,227,260,305,309,314,331,342],"synthetic":[8,264],"molecules":[9,316],"to":[10,56,152,159,164,175,186,241,282,346],"proteins":[11,183,202,245,262],"inside":[12],"living":[13,204,247,348],"cells.":[14,39,141,248],"A":[15],"frontier":[16],"this":[18,75],"field":[19],"is":[20],"designing":[21],"multifunctional":[22,90,145,229,293,344],"ligands":[23,42,59,91,121,147,267,294,345],"that":[24,98,120,171,271,292],"contain":[25],"both":[26],"fluorophores":[27],"and":[28,34,79,129,198,206,211,243,256,288,301,334],"affinity":[29,196,303],"or":[31,155,167,307],"pharmacological":[32],"agents":[33],"can":[35,46,63,184],"still":[36],"efficiently":[37,139,298],"enter":[38,47,140],"Self-labeling":[40],"tag":[41],"with":[43,60,111,148,263],"short":[44],"linkers":[45,62],"cells":[48,179,205,300],"readily":[49],"but":[50,67],"often":[51],"show":[52,68],"less":[53],"activity":[54],"due":[55,281],"steric":[57],"issues;":[58],"long":[61],"be":[64,318,326],"more":[65,273],"potent":[66],"lower":[69],"cell":[70,88,286],"permeability.":[71],"Here,":[72],"we":[73],"overcome":[74],"tug-of-war":[76],"between":[77,285],"efficacy":[78],"cell-permeability":[80],"by":[81,320],"devising":[82],"a":[83,149,156,329],"rational":[84],"strategy":[85],"for":[86,92,232,268,328],"making":[87],"permeable":[89],"labeling":[93,250],"HaloTag":[94,146,255],"fusions.":[95],"We":[96,142,169,290],"found":[97],"the":[99,165,207,225,254,312,340],"lactone\u2013zwitterion":[100],"equilibrium":[101],"sconstant":[102],"(":[103],"K":[104,127,214],"L\u2013Z":[105,128],")":[106],"rhodamines":[108],"inversely":[109],"correlates":[110],"their":[112],"distribution":[113],"coefficients":[114],"(log":[115],"D":[116,132,219],"7.4":[117,133,220],"),":[118],"suggesting":[119],"based":[122,295],"on":[123,296],"dyes":[124],"exhibiting":[125],"low":[126,213],"high":[130],"log":[131,218],"values,":[134],"such":[135,252],"as":[136,253],"Si-rhodamines,":[137],"would":[138],"designed":[143],"cell-permeable":[144],"biotin":[150],"moiety":[151],"purify":[153],"mitochondria":[154,306],"JQ1":[157],"appendage":[158],"translocate":[160],"BRD4":[161,174],"from":[162],"euchromatin":[163],"nucleolus":[166],"heterochromatin.":[168],"discovered":[170,291],"translocation":[172,199,308],"constitutive":[176],"heterochromatin":[177],"in":[178,189,203,246],"expressing":[180],"HaloTag\u2013HP1a":[181],"fusion":[182],"lead":[185],"apparent":[187],"increases":[188],"transcriptional":[190],"activity.":[191],"These":[192,323],"new":[193,228],"reagents":[194],"capture":[197],"intracellular":[201],"use":[208],"Si-rhodamines":[210,297],"other":[212,343],"L\u2013":[215],"Z":[216],"/high":[217],"dye":[221],"scaffolds":[222],"will":[223,338],"facilitate":[224],"design":[226,341],"chemical":[230,276],"tools":[231,240],"biology.":[233],"SIGNIFICANCE":[234],"STATEMENT":[235],"Understanding":[236],"cellular":[237,261],"processes":[238],"requires":[239],"measure":[242],"manipulate":[244],"Self-":[249],"tags,":[251],"SNAP-tag,":[257],"modification":[259],"molecules.":[265],"Creating":[266],"these":[269,315],"systems":[270],"have":[272],"than":[274],"one":[275],"motif":[277],"remains":[278],"challenging,":[279],"however,":[280],"competing":[283],"demands":[284],"permeability":[287],"functionality.":[289],"entered":[299],"enabled":[302],"purification":[304],"nuclear":[310],"proteins;":[311],"performance":[313],"could":[317],"verified":[319],"fluorescence":[321],"microscopy.":[322],"compounds":[324],"should":[325],"useful":[327],"variety":[330],"biological":[332],"experiments":[333],"our":[335],"general":[336],"framework":[337],"allow":[339],"study":[347],"systems.":[349]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1}],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2022-07-04T00:00:00"}