{"id":"https://openalex.org/W4283592086","doi":"https://doi.org/10.26434/chemrxiv-2022-xlspx","title":"Amplifying ion signal levels for MS/MS and MSn applications in native MS experiments in negative ion mode using cVSSI techniques","display_name":"Amplifying ion signal levels for MS/MS and MSn applications in native MS experiments in negative ion mode using cVSSI techniques","publication_year":2022,"publication_date":"2022-06-27","ids":{"openalex":"https://openalex.org/W4283592086","doi":"https://doi.org/10.26434/chemrxiv-2022-xlspx"},"language":"en","primary_location":{"id":"doi:10.26434/chemrxiv-2022-xlspx","doi":"https://doi.org/10.26434/chemrxiv-2022-xlspx","is_oa":true,"landing_page_url":"https://doi.org/10.26434/chemrxiv-2022-xlspx","pdf_url":"https://chemrxiv.org/engage/api-gateway/chemrxiv/assets/orp/resource/item/62b4914258b3d6263f5be1c0/original/amplifying-ion-signal-levels-for-ms-ms-and-m-sn-applications-in-native-ms-experiments-in-negative-ion-mode-using-c-vssi-techniques.pdf","source":{"id":"https://openalex.org/S4393918830","display_name":"ChemRxiv","issn_l":"2573-2293","issn":["2573-2293"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"},"volume":null,"type":"preprint","indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://chemrxiv.org/engage/api-gateway/chemrxiv/assets/orp/resource/item/62b4914258b3d6263f5be1c0/original/amplifying-ion-signal-levels-for-ms-ms-and-m-sn-applications-in-native-ms-experiments-in-negative-ion-mode-using-c-vssi-techniques.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5006368800","display_name":"Kushani Attanayake","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I12097938","display_name":"West Virginia University","ror":"https://ror.org/011vxgd24","country_code":"US","type":"education","lineage":["https://openalex.org/I12097938"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kushani Attanayake","raw_affiliation_strings":["West Virginia University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"West Virginia University","institution_ids":["https://openalex.org/I12097938"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100424938","display_name":"Chong Li","orcid":"https://orcid.org/0009-0000-3015-181X","observed_orcids":["https://orcid.org/0009-0000-3015-181X"]},"institutions":[{"id":"https://openalex.org/I12097938","display_name":"West Virginia University","ror":"https://ror.org/011vxgd24","country_code":"US","type":"education","lineage":["https://openalex.org/I12097938"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Chong Li","raw_affiliation_strings":["West Virginia University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"West Virginia University","institution_ids":["https://openalex.org/I12097938"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015345838","display_name":"Daud Sharif","orcid":"https://orcid.org/0000-0001-8397-0536","observed_orcids":["https://orcid.org/0000-0001-8397-0536"]},"institutions":[{"id":"https://openalex.org/I12097938","display_name":"West Virginia University","ror":"https://ror.org/011vxgd24","country_code":"US","type":"education","lineage":["https://openalex.org/I12097938"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Daud Sharif","raw_affiliation_strings":["West Virginia University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"West Virginia University","institution_ids":["https://openalex.org/I12097938"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100432738","display_name":"Peng Li","orcid":"https://orcid.org/0000-0002-8332-7142","observed_orcids":["https://orcid.org/0000-0002-8332-7142","https://orcid.org/0000-0002-5235-6311"]},"institutions":[{"id":"https://openalex.org/I12097938","display_name":"West Virginia University","ror":"https://ror.org/011vxgd24","country_code":"US","type":"education","lineage":["https://openalex.org/I12097938"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Peng Li","raw_affiliation_strings":["West Virginia University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"West Virginia University","institution_ids":["https://openalex.org/I12097938"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5037830220","display_name":"Stephen J. Valentine","orcid":"https://orcid.org/0000-0003-4360-2928","observed_orcids":["https://orcid.org/0000-0003-4360-2928"]},"institutions":[{"id":"https://openalex.org/I12097938","display_name":"West Virginia University","ror":"https://ror.org/011vxgd24","country_code":"US","type":"education","lineage":["https://openalex.org/I12097938"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Stephen Valentine","raw_affiliation_strings":["West Virginia University"],"raw_orcid":"https://orcid.org/0000-0003-4360-2928","affiliations":[{"raw_affiliation_string":"West Virginia University","institution_ids":["https://openalex.org/I12097938"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I12097938"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":1,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12776","display_name":"Electrohydrodynamics and Fluid Dynamics","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2208","display_name":"Electrical and Electronic Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/native-mass-spectrometry","display_name":"native mass spectrometry","score":0.9229999780654907},{"id":"https://openalex.org/keywords/negative-ion-mode","display_name":"negative ion mode","score":0.9599999785423279},{"id":"https://openalex.org/keywords/tandem-mass-spectrometry","display_name":"tandem mass spectrometry","score":0.972000002861023},{"id":"https://openalex.org/keywords/signal-to-noise-ratio","display_name":"signal-to-noise ratio","score":0.8389999866485596},{"id":"https://openalex.org/keywords/fragment-ion-identification","display_name":"fragment ion identification","score":0.6830000281333923},{"id":"https://openalex.org/keywords/heated-electrospray-ionization","display_name":"heated electrospray ionization","score":0.9620000123977661}],"concepts":[{"id":"https://openalex.org/C145148216","wikidata":"https://www.wikidata.org/wiki/Q36496","display_name":"Ion","level":2,"score":0.7683811783790588},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7683409452438354},{"id":"https://openalex.org/C198291218","wikidata":"https://www.wikidata.org/wiki/Q190382","display_name":"Ionization","level":3,"score":0.7093517184257507},{"id":"https://openalex.org/C40684141","wikidata":"https://www.wikidata.org/wiki/Q902061","display_name":"Electrospray ionization","level":3,"score":0.7043490409851074},{"id":"https://openalex.org/C113196181","wikidata":"https://www.wikidata.org/wiki/Q485223","display_name":"Analytical Chemistry (journal)","level":2,"score":0.6153600811958313},{"id":"https://openalex.org/C188224857","wikidata":"https://www.wikidata.org/wiki/Q905291","display_name":"Ion source","level":3,"score":0.5768532156944275},{"id":"https://openalex.org/C162356407","wikidata":"https://www.wikidata.org/wiki/Q180809","display_name":"Mass spectrometry","level":2,"score":0.5738118290901184},{"id":"https://openalex.org/C10390740","wikidata":"https://www.wikidata.org/wiki/Q2282401","display_name":"Analyte","level":2,"score":0.5679960250854492},{"id":"https://openalex.org/C31827203","wikidata":"https://www.wikidata.org/wiki/Q874296","display_name":"Tandem mass spectrometry","level":3,"score":0.5475200414657593},{"id":"https://openalex.org/C49853544","wikidata":"https://www.wikidata.org/wiki/Q206229","display_name":"Biomolecule","level":2,"score":0.41884803771972656},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.29994797706604004},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.26434/chemrxiv-2022-xlspx","doi":"https://doi.org/10.26434/chemrxiv-2022-xlspx","is_oa":true,"landing_page_url":"https://doi.org/10.26434/chemrxiv-2022-xlspx","pdf_url":"https://chemrxiv.org/engage/api-gateway/chemrxiv/assets/orp/resource/item/62b4914258b3d6263f5be1c0/original/amplifying-ion-signal-levels-for-ms-ms-and-m-sn-applications-in-native-ms-experiments-in-negative-ion-mode-using-c-vssi-techniques.pdf","source":{"id":"https://openalex.org/S4393918830","display_name":"ChemRxiv","issn_l":"2573-2293","issn":["2573-2293"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.26434/chemrxiv-2022-xlspx","doi":"https://doi.org/10.26434/chemrxiv-2022-xlspx","is_oa":true,"landing_page_url":"https://doi.org/10.26434/chemrxiv-2022-xlspx","pdf_url":"https://chemrxiv.org/engage/api-gateway/chemrxiv/assets/orp/resource/item/62b4914258b3d6263f5be1c0/original/amplifying-ion-signal-levels-for-ms-ms-and-m-sn-applications-in-native-ms-experiments-in-negative-ion-mode-using-c-vssi-techniques.pdf","source":{"id":"https://openalex.org/S4393918830","display_name":"ChemRxiv","issn_l":"2573-2293","issn":["2573-2293"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1535578731","display_name":"Development of VSSI-probe technology for in situ probing biological systems using mass spectrometry","funder_award_id":"R01GM135432","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4283592086.pdf","grobid_xml":"https://content.openalex.org/works/W4283592086.grobid-xml"},"referenced_works_count":44,"referenced_works":["https://openalex.org/W1145214654","https://openalex.org/W1967255819","https://openalex.org/W1977853483","https://openalex.org/W1980679837","https://openalex.org/W1981978551","https://openalex.org/W1995693931","https://openalex.org/W1996518786","https://openalex.org/W2000983103","https://openalex.org/W2008277785","https://openalex.org/W2021809952","https://openalex.org/W2032284401","https://openalex.org/W2034541601","https://openalex.org/W2038284852","https://openalex.org/W2052365939","https://openalex.org/W2062824542","https://openalex.org/W2069839371","https://openalex.org/W2073292063","https://openalex.org/W2074877005","https://openalex.org/W2081222587","https://openalex.org/W2084130233","https://openalex.org/W2089686959","https://openalex.org/W2090006460","https://openalex.org/W2110174460","https://openalex.org/W2112673374","https://openalex.org/W2120977203","https://openalex.org/W2124081847","https://openalex.org/W2124157967","https://openalex.org/W2145374602","https://openalex.org/W2170015710","https://openalex.org/W2296260051","https://openalex.org/W2314545667","https://openalex.org/W2341349155","https://openalex.org/W2410310292","https://openalex.org/W2442564305","https://openalex.org/W2550535012","https://openalex.org/W2558396410","https://openalex.org/W2750322303","https://openalex.org/W2807885489","https://openalex.org/W2829097635","https://openalex.org/W3000429282","https://openalex.org/W3162600333","https://openalex.org/W4234357073","https://openalex.org/W6635715184","https://openalex.org/W6637370835"],"related_works":["https://openalex.org/W4200356007","https://openalex.org/W4293828048","https://openalex.org/W3118575243","https://openalex.org/W7134275100","https://openalex.org/W2915366856","https://openalex.org/W7165372686","https://openalex.org/W4283260738","https://openalex.org/W4307804891","https://openalex.org/W4200435317","https://openalex.org/W4408903770"],"abstract_inverted_index":{"Capillary":[0],"vibrating":[1],"sharp-edge":[2],"spray":[3],"ionization":[4,101,136],"(cVSSI)":[5],"has":[6],"been":[7],"investigated":[8],"for":[9,23,127,179,181],"its":[10],"utility":[11],"in":[12,27,48,58,161],"tandem":[13,19],"mass":[14],"spectrometry":[15],"(MS)":[16],"and":[17,36,158],"multistage":[18],"MS":[20,25],"(MSn)":[21],"experiments":[22,72,169],"native":[24],"analyses":[26],"negative":[28],"ion":[29,49,92,148,164,173],"mode.":[30],"Compared":[31],"with":[32,99,133],"capillary":[33],"electrosprayionization":[34],"(cESI)":[35],"heated":[37],"electrospray":[38],"ionization,":[39],"cVSSI":[40,61,88,129,153],"provided":[41,151],"significant":[42],"increases":[43],"(up":[44],"to":[45,67,156,190],">1000":[46],"fold)":[47],"signal":[50,149,174],"levels.":[51],"Signal-to-noise":[52],"ratio":[53],"(S/N)":[54],"show":[55],"a":[56,116],"reduction":[57],"difference":[59],"where":[60],"provides":[62],"an":[63,122],"increase":[64],"of":[65,95,107,118,145,163,185],"up":[66],"7":[68],"fold.":[69],"Performing":[70],"MS3":[71],"on":[73],"all":[74,100],"biomolecules":[75],"examined":[76],"here":[77],"showed":[78],"that":[79,171],"more":[80],"fragment":[81,96],"ions":[82,97,114],"could":[83],"be":[84],"identified":[85],"from":[86],"the":[87,91,104,108,128,134,140,143,146,172],"experiments.":[89],"Additionally,":[90],"intensity":[93],"levels":[94],"observed":[98],"sources":[102],"exceeds":[103],"S/N":[105],"advantage":[106,126,150],"precursor":[109],"ions.":[110],"Tracking":[111],"MS/MS":[112],"product":[113],"as":[115],"function":[117],"analyte":[119],"concentration":[120],"reveals":[121],"~10":[123],"fold":[124],"sensitivity":[125],"approach":[130],"when":[131],"compared":[132],"other":[135],"sources.":[137],"Taken":[138],"together,":[139],"results":[141],"highlight":[142],"importance":[144],"total":[147],"by":[152],"(as":[154],"opposed":[155],"S/N)":[157],"are":[159,188],"discussed":[160],"light":[162],"utilization":[165],"considerations.":[166],"Finally,":[167],"separate":[168],"demonstrate":[170],"level":[175],"advantages":[176],"hold":[177],"even":[178],"samples":[180],"which":[182],"small":[183],"amounts":[184],"organic":[186],"solvent":[187],"used":[189],"improve":[191],"ionization.":[192]},"counts_by_year":[{"year":2025,"cited_by_count":1}],"updated_date":"2026-10-30T12:39:25.321706","created_date":"2022-06-28T00:00:00"}