{"id":"https://openalex.org/W4283527463","doi":"https://doi.org/10.1016/j.lwt.2022.113705","title":"Molecular cloning, expression and characterization of a bacteriophage JN01 endolysin and its antibacterial activity against E. coli O157:H7","display_name":"Molecular cloning, expression and characterization of a bacteriophage JN01 endolysin and its antibacterial activity against E. coli O157:H7","publication_year":2022,"publication_date":"2022-06-26","ids":{"openalex":"https://openalex.org/W4283527463","doi":"https://doi.org/10.1016/j.lwt.2022.113705"},"language":"en","primary_location":{"id":"doi:10.1016/j.lwt.2022.113705","doi":"https://doi.org/10.1016/j.lwt.2022.113705","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.lwt.2022.113705","pdf_url":"https://www.sciencedirect.com/science/article/pii/S0023643822006405/pdf","source":{"id":"https://openalex.org/S26760650","display_name":"LWT","issn_l":"0023-6438","issn":["0023-6438","1096-1127"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","norway-1","oarl","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"LWT","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.sciencedirect.com/science/article/pii/S0023643822006405/pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5016184267","display_name":"Kaisheng Shen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I74837319","display_name":"Jiangxi Agricultural University","ror":"https://ror.org/00dc7s858","country_code":"CN","type":"education","lineage":["https://openalex.org/I74837319"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Kai-Sheng Shen","raw_affiliation_strings":["College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang, 330045, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang, 330045, China","institution_ids":["https://openalex.org/I74837319"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5085763890","display_name":"Mei Shu","orcid":"https://orcid.org/0000-0001-6026-5914","observed_orcids":["https://orcid.org/0000-0001-6026-5914"]},"institutions":[{"id":"https://openalex.org/I74837319","display_name":"Jiangxi Agricultural University","ror":"https://ror.org/00dc7s858","country_code":"CN","type":"education","lineage":["https://openalex.org/I74837319"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Mei Shu","raw_affiliation_strings":["College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang, 330045, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang, 330045, China","institution_ids":["https://openalex.org/I74837319"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5050203200","display_name":"Meng-Xuan Tang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I74837319","display_name":"Jiangxi Agricultural University","ror":"https://ror.org/00dc7s858","country_code":"CN","type":"education","lineage":["https://openalex.org/I74837319"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Meng-Xuan Tang","raw_affiliation_strings":["College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang, 330045, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang, 330045, China","institution_ids":["https://openalex.org/I74837319"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5018044499","display_name":"Wenyu Yang","orcid":"https://orcid.org/0000-0001-5885-3450","observed_orcids":["https://orcid.org/0000-0001-5885-3450"]},"institutions":[{"id":"https://openalex.org/I74837319","display_name":"Jiangxi Agricultural University","ror":"https://ror.org/00dc7s858","country_code":"CN","type":"education","lineage":["https://openalex.org/I74837319"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Wen-Yu Yang","raw_affiliation_strings":["College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang, 330045, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang, 330045, China","institution_ids":["https://openalex.org/I74837319"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5051000479","display_name":"Shuchao Wang","orcid":"https://orcid.org/0000-0001-9782-9656","observed_orcids":["https://orcid.org/0000-0001-9782-9656"]},"institutions":[{"id":"https://openalex.org/I74837319","display_name":"Jiangxi Agricultural University","ror":"https://ror.org/00dc7s858","country_code":"CN","type":"education","lineage":["https://openalex.org/I74837319"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Shu-Chao Wang","raw_affiliation_strings":["College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang, 330045, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang, 330045, China","institution_ids":["https://openalex.org/I74837319"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103070950","display_name":"Chan Zhong","orcid":"https://orcid.org/0000-0001-8737-9161","observed_orcids":["https://orcid.org/0000-0001-8737-9161"]},"institutions":[{"id":"https://openalex.org/I74837319","display_name":"Jiangxi Agricultural University","ror":"https://ror.org/00dc7s858","country_code":"CN","type":"education","lineage":["https://openalex.org/I74837319"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Chan Zhong","raw_affiliation_strings":["College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang, 330045, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang, 330045, China","institution_ids":["https://openalex.org/I74837319"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5063751100","display_name":"Guoping Wu","orcid":"https://orcid.org/0000-0001-7797-7627","observed_orcids":["https://orcid.org/0000-0001-7797-7627"]},"institutions":[{"id":"https://openalex.org/I74837319","display_name":"Jiangxi Agricultural University","ror":"https://ror.org/00dc7s858","country_code":"CN","type":"education","lineage":["https://openalex.org/I74837319"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Guo-Ping Wu","raw_affiliation_strings":["College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang, 330045, China"],"raw_orcid":"https://orcid.org/0000-0001-7797-7627","affiliations":[{"raw_affiliation_string":"College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang, 330045, China","institution_ids":["https://openalex.org/I74837319"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5063751100"],"corresponding_institution_ids":["https://openalex.org/I74837319"],"apc_list":{"value":2250,"currency":"USD","value_usd":2250},"apc_paid":{"value":2250,"currency":"USD","value_usd":2250},"fwci":1.5956,"has_fulltext":true,"cited_by_count":24,"citation_normalized_percentile":{"value":0.8465952,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"165","issue":null,"first_page":"113705","last_page":"113705"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11048","display_name":"Bacteriophages and microbial interactions","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/2406","display_name":"Virology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11048","display_name":"Bacteriophages and microbial interactions","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/2406","display_name":"Virology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10900","display_name":"Listeria monocytogenes in Food Safety","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1106","display_name":"Food Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10943","display_name":"Escherichia coli research studies","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/bacteriophage-endolysins","display_name":"bacteriophage endolysins","score":0.871999979019165},{"id":"https://openalex.org/keywords/escherichia-coli-o157-h7","display_name":"Escherichia coli O157:H7","score":0.9900000095367432},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.8640000224113464},{"id":"https://openalex.org/keywords/antibacterial-activity","display_name":"antibacterial activity","score":0.7279999852180481},{"id":"https://openalex.org/keywords/edta","display_name":"EDTA","score":0.4690000116825104},{"id":"https://openalex.org/keywords/lettuce-contamination","display_name":"lettuce contamination","score":0.6240000128746033},{"id":"https://openalex.org/keywords/food-safety","display_name":"food safety","score":0.6990000009536743},{"id":"https://openalex.org/keywords/biological-control","display_name":"biological control","score":0.5730000138282776},{"id":"https://openalex.org/keywords/gram-negative-bacteria","display_name":"Gram-negative bacteria","score":0.5910000205039978},{"id":"https://openalex.org/keywords/thermal-stability","display_name":"thermal stability","score":0.5260000228881836},{"id":"https://openalex.org/keywords/ph-stability","display_name":"pH stability","score":0.6430000066757202}],"concepts":[{"id":"https://openalex.org/C22538091","wikidata":"https://www.wikidata.org/wiki/Q2646830","display_name":"Lysin","level":5,"score":0.8068356513977051},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.7946571111679077},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.7042576670646667},{"id":"https://openalex.org/C182615771","wikidata":"https://www.wikidata.org/wiki/Q1369386","display_name":"Lytic cycle","level":3,"score":0.6893205642700195},{"id":"https://openalex.org/C2776441376","wikidata":"https://www.wikidata.org/wiki/Q165028","display_name":"Bacteriophage","level":4,"score":0.6855982542037964},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.6441779136657715},{"id":"https://openalex.org/C2776966407","wikidata":"https://www.wikidata.org/wiki/Q24779410","display_name":"Lysozyme","level":2,"score":0.6436935663223267},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.573806881904602},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.54766845703125},{"id":"https://openalex.org/C2777637488","wikidata":"https://www.wikidata.org/wiki/Q31856","display_name":"Pseudomonas aeruginosa","level":3,"score":0.5313376188278198},{"id":"https://openalex.org/C4937899","wikidata":"https://www.wikidata.org/wiki/Q68541106","display_name":"Antimicrobial","level":2,"score":0.5008304119110107},{"id":"https://openalex.org/C2781065037","wikidata":"https://www.wikidata.org/wiki/Q150839","display_name":"Salmonella","level":3,"score":0.4822567105293274},{"id":"https://openalex.org/C57409179","wikidata":"https://www.wikidata.org/wiki/Q1218293","display_name":"Lysis","level":2,"score":0.45494651794433594},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.34149888157844543},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.22436612844467163},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.15393167734146118},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.1309034824371338},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.06725805997848511},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1016/j.lwt.2022.113705","doi":"https://doi.org/10.1016/j.lwt.2022.113705","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.lwt.2022.113705","pdf_url":"https://www.sciencedirect.com/science/article/pii/S0023643822006405/pdf","source":{"id":"https://openalex.org/S26760650","display_name":"LWT","issn_l":"0023-6438","issn":["0023-6438","1096-1127"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","norway-1","oarl","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"LWT","raw_type":"journal-article"}],"best_oa_location":{"id":"doi:10.1016/j.lwt.2022.113705","doi":"https://doi.org/10.1016/j.lwt.2022.113705","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.lwt.2022.113705","pdf_url":"https://www.sciencedirect.com/science/article/pii/S0023643822006405/pdf","source":{"id":"https://openalex.org/S26760650","display_name":"LWT","issn_l":"0023-6438","issn":["0023-6438","1096-1127"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","norway-1","oarl","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"LWT","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G4578667917","display_name":null,"funder_award_id":"32160608","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G7937417795","display_name":null,"funder_award_id":"20204BCJL22045","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"}],"funders":[{"id":"https://openalex.org/F4320321001","display_name":"National Natural Science Foundation of China","ror":"https://ror.org/01h0zpd94"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4283527463.pdf","grobid_xml":"https://content.openalex.org/works/W4283527463.grobid-xml"},"referenced_works_count":32,"referenced_works":["https://openalex.org/W2031752327","https://openalex.org/W2051765619","https://openalex.org/W2152343141","https://openalex.org/W2156431883","https://openalex.org/W2395104290","https://openalex.org/W2413905873","https://openalex.org/W2512258079","https://openalex.org/W2563850592","https://openalex.org/W2589200409","https://openalex.org/W2803521541","https://openalex.org/W2912256178","https://openalex.org/W2949197294","https://openalex.org/W2954865617","https://openalex.org/W2957021304","https://openalex.org/W2986698776","https://openalex.org/W3033236495","https://openalex.org/W3047006804","https://openalex.org/W3082156002","https://openalex.org/W3092273878","https://openalex.org/W3118839110","https://openalex.org/W3135585183","https://openalex.org/W3136568118","https://openalex.org/W3157788392","https://openalex.org/W3177879102","https://openalex.org/W3191390148","https://openalex.org/W3199993416","https://openalex.org/W4206287074","https://openalex.org/W6734208513","https://openalex.org/W6765646059","https://openalex.org/W6782008074","https://openalex.org/W6791273992","https://openalex.org/W6805639302"],"related_works":["https://openalex.org/W4364352576","https://openalex.org/W3044612503","https://openalex.org/W7171453282","https://openalex.org/W2006571639","https://openalex.org/W3135585183","https://openalex.org/W3043877009","https://openalex.org/W4400387275","https://openalex.org/W2016761629","https://openalex.org/W3177879102","https://openalex.org/W2937089792"],"abstract_inverted_index":{"Endolysins":[0],"encoded":[1],"by":[2,150],"bacteriophages":[3],"are":[4],"considered":[5],"bacteriostatic":[6,185],"agents":[7],"for":[8,167,187],"the":[9,28,37,138],"prevention":[10],"of":[11,40,59,67,73,123,140,159,189],"pathogenic":[12],"bacteria":[13],"proliferations.":[14],"In":[15],"this":[16],"study,":[17],"a":[18,60,64,81,120,157,181],"possible":[19],"endolysin":[20],"from":[21],"bacteriophage":[22],"JN01,":[23],"namely":[24],"LysJN01,":[25],"belonging":[26],"to":[27],"lysozyme-like":[29],"superfamily,":[30],"was":[31,48,75,147],"cloned,":[32],"expressed":[33],"and":[34,53,113,134,152,163,170,183],"characterized.":[35],"Meanwhile,":[36],"bactericidal":[38],"activity":[39,84,107,125],"recombinant":[41],"LysJN01":[42],"(rLysJN01)":[43],"against":[44,85,126],"E.":[45,88,99,131,141,190],"coli":[46,89,100,142,191],"O157:H7":[47,143],"evaluated":[49],"in":[50],"Tris-HCl":[51],"buffer":[52],"on":[54,144],"lettuce.":[55],"Recombinant":[56],"LysJN01(rLysJN01)":[57],"consists":[58],"single":[61],"domain":[62],"with":[63,93,156],"molecular":[65],"weight":[66],"about":[68,76],"20":[69,77],"kDa.":[70],"The":[71],"yield":[72],"rLysJN01":[74,79,162,179],"mg/L":[78],"had":[80],"strong":[82],"lytic":[83],"chloroform":[86],"treated":[87],"O157:H7.":[90,101,192],"It":[91],"combined":[92],"EDTA":[94,166],"could":[95],"also":[96,118],"significantly":[97,148],"lyse":[98],"Afterward,":[102],"rlysJN01":[103],"showed":[104],"high":[105],"enzymatic":[106],"stability":[108],"at":[109],"temperature":[110],"(4\u201395":[111],"OC)":[112],"pH":[114],"(5.0\u201311.0).":[115],"Moreover,":[116],"it":[117],"revealed":[119],"broad":[121],"spectrum":[122],"antimicrobial":[124],"Gram-negative":[127],"pathogens,":[128],"such":[129],"as":[130],"coli,":[132],"Salmonella":[133],"Pseudomonas":[135],"aeruginosa.":[136],"Furthermore,":[137],"counts":[139],"contaminated":[145],"lettuce":[146],"reduced":[149],"91.6%":[151],"97.8%":[153],"after":[154],"treatment":[155],"combination":[158],"60":[160,171],"\u03bcg/mL":[161],"0.5":[164],"mmol/L":[165],"30":[168],"min":[169],"min,":[172],"respectively.":[173],"All":[174],"these":[175],"results":[176],"indicated":[177],"that":[178],"is":[180],"potential":[182],"efficient":[184],"agent":[186],"biocontrol":[188]},"counts_by_year":[{"year":2026,"cited_by_count":3},{"year":2025,"cited_by_count":9},{"year":2024,"cited_by_count":5},{"year":2023,"cited_by_count":6},{"year":2022,"cited_by_count":1}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2022-06-27T00:00:00"}