{"id":"https://openalex.org/W4282976247","doi":"https://doi.org/10.1158/1538-7445.am2022-2817","title":"Abstract 2817: GD2 CAR-T cells engineered using retroviral transduction or CRISPR editing exhibit strong cytolytic potency against glioma stem cells","display_name":"Abstract 2817: GD2 CAR-T cells engineered using retroviral transduction or CRISPR editing exhibit strong cytolytic potency against glioma stem cells","publication_year":2022,"publication_date":"2022-06-15","ids":{"openalex":"https://openalex.org/W4282976247","doi":"https://doi.org/10.1158/1538-7445.am2022-2817"},"language":"en","primary_location":{"id":"doi:10.1158/1538-7445.am2022-2817","doi":"https://doi.org/10.1158/1538-7445.am2022-2817","is_oa":false,"landing_page_url":"https://doi.org/10.1158/1538-7445.am2022-2817","pdf_url":null,"source":{"id":"https://openalex.org/S168522863","display_name":"Cancer Research","issn_l":"0008-5472","issn":["0008-5472","1538-7445"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320273","host_organization_name":"American Association for Cancer Research","host_organization_lineage":["https://openalex.org/P4310320273"],"host_organization_lineage_names":["American Association for Cancer Research"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer Research","raw_type":"journal-article"},"volume":null,"type":"conference-abstract","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5040919958","display_name":"Stacie A. Chvatal","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210167161","display_name":"Axion Biosystems (United States)","ror":"https://ror.org/05w8qnb04","country_code":"US","type":"company","lineage":["https://openalex.org/I4210167161"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Stacie A. Chvatal","raw_affiliation_strings":["1Axion BioSystems, Atlanta, GA;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Axion BioSystems, Atlanta, GA;","institution_ids":["https://openalex.org/I4210167161"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5011060620","display_name":"Meghan T. Logun","orcid":"https://orcid.org/0000-0002-7274-811X","observed_orcids":["https://orcid.org/0000-0002-7274-811X"]},"institutions":[{"id":"https://openalex.org/I165733156","display_name":"University of Georgia","ror":"https://ror.org/00te3t702","country_code":"US","type":"education","lineage":["https://openalex.org/I165733156"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Meghan T. Logun","raw_affiliation_strings":["2University of Georgia, Athens, GA;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"2University of Georgia, Athens, GA;","institution_ids":["https://openalex.org/I165733156"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5062656470","display_name":"Denise Sullivan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210167161","display_name":"Axion Biosystems (United States)","ror":"https://ror.org/05w8qnb04","country_code":"US","type":"company","lineage":["https://openalex.org/I4210167161"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Denise D. Sullivan","raw_affiliation_strings":["1Axion BioSystems, Atlanta, GA;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Axion BioSystems, Atlanta, GA;","institution_ids":["https://openalex.org/I4210167161"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5080188661","display_name":"Heather Brant Hayes","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210167161","display_name":"Axion Biosystems (United States)","ror":"https://ror.org/05w8qnb04","country_code":"US","type":"company","lineage":["https://openalex.org/I4210167161"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Heather B. Hayes","raw_affiliation_strings":["1Axion BioSystems, Atlanta, GA;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Axion BioSystems, Atlanta, GA;","institution_ids":["https://openalex.org/I4210167161"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5034769473","display_name":"Daniel C. Millard","orcid":"https://orcid.org/0000-0002-1416-3148","observed_orcids":["https://orcid.org/0000-0002-1416-3148"]},"institutions":[{"id":"https://openalex.org/I4210167161","display_name":"Axion Biosystems (United States)","ror":"https://ror.org/05w8qnb04","country_code":"US","type":"company","lineage":["https://openalex.org/I4210167161"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Daniel Millard","raw_affiliation_strings":["1Axion BioSystems, Atlanta, GA;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Axion BioSystems, Atlanta, GA;","institution_ids":["https://openalex.org/I4210167161"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5058371134","display_name":"Mueller P. Katie","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I135310074","display_name":"University of Wisconsin\u2013Madison","ror":"https://ror.org/01y2jtd41","country_code":"US","type":"education","lineage":["https://openalex.org/I135310074"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Mueller P. Katie","raw_affiliation_strings":["3University of Wisconsin-Madison, Madison, WI;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"3University of Wisconsin-Madison, Madison, WI;","institution_ids":["https://openalex.org/I135310074"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5068272337","display_name":"Nicole J. Piscopo","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I135310074","display_name":"University of Wisconsin\u2013Madison","ror":"https://ror.org/01y2jtd41","country_code":"US","type":"education","lineage":["https://openalex.org/I135310074"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Nicole J. Piscopo","raw_affiliation_strings":["3University of Wisconsin-Madison, Madison, WI;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"3University of Wisconsin-Madison, Madison, WI;","institution_ids":["https://openalex.org/I135310074"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5063923902","display_name":"Amritava Das","orcid":"https://orcid.org/0000-0001-7227-8472","observed_orcids":["https://orcid.org/0000-0001-7227-8472"]},"institutions":[{"id":"https://openalex.org/I135310074","display_name":"University of Wisconsin\u2013Madison","ror":"https://ror.org/01y2jtd41","country_code":"US","type":"education","lineage":["https://openalex.org/I135310074"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Amritava Das","raw_affiliation_strings":["3University of Wisconsin-Madison, Madison, WI;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"3University of Wisconsin-Madison, Madison, WI;","institution_ids":["https://openalex.org/I135310074"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5087358894","display_name":"Krishanu Saha","orcid":"https://orcid.org/0000-0003-2837-0858","observed_orcids":["https://orcid.org/0000-0003-2837-0858"]},"institutions":[{"id":"https://openalex.org/I135310074","display_name":"University of Wisconsin\u2013Madison","ror":"https://ror.org/01y2jtd41","country_code":"US","type":"education","lineage":["https://openalex.org/I135310074"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kris Saha","raw_affiliation_strings":["3University of Wisconsin-Madison, Madison, WI;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"3University of Wisconsin-Madison, Madison, WI;","institution_ids":["https://openalex.org/I135310074"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5078484855","display_name":"Daniel Jay Brat","orcid":"https://orcid.org/0000-0002-0633-6563","observed_orcids":["https://orcid.org/0000-0002-0633-6563"]},"institutions":[{"id":"https://openalex.org/I111979921","display_name":"Northwestern University","ror":"https://ror.org/000e0be47","country_code":"US","type":"education","lineage":["https://openalex.org/I111979921"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Daniel J. Brat","raw_affiliation_strings":["4Northwestern University, Chicago, IL;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"4Northwestern University, Chicago, IL;","institution_ids":["https://openalex.org/I111979921"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5072097405","display_name":"Lohitash Karumbaiah","orcid":"https://orcid.org/0000-0001-7969-417X","observed_orcids":["https://orcid.org/0000-0001-7969-417X"]},"institutions":[{"id":"https://openalex.org/I165733156","display_name":"University of Georgia","ror":"https://ror.org/00te3t702","country_code":"US","type":"education","lineage":["https://openalex.org/I165733156"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Lohitash Karumbaiah","raw_affiliation_strings":["5University of Georgia, Atlanta, GA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"5University of Georgia, Atlanta, GA","institution_ids":["https://openalex.org/I165733156"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"82","issue":"12_Supplement","first_page":"2817","last_page":"2817"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11491","display_name":"CAR-T cell therapy research","score":0.9901999831199646,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11491","display_name":"CAR-T cell therapy research","score":0.9901999831199646,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10129","display_name":"Glioma Diagnosis and Treatment","score":0.006399999838322401,"subfield":{"id":"https://openalex.org/subfields/2716","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10580","display_name":"Immunotherapy and Immune Responses","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/gd2-car-t-cells","display_name":"GD2 CAR T cells","score":0.8939999938011169},{"id":"https://openalex.org/keywords/glioblastoma","display_name":"glioblastoma","score":0.9049999713897705},{"id":"https://openalex.org/keywords/glioma-stem-cells","display_name":"glioma stem cells","score":0.9990000128746033},{"id":"https://openalex.org/keywords/retroviral-transduction","display_name":"retroviral transduction","score":0.8809999823570251},{"id":"https://openalex.org/keywords/crispr-gene-editing","display_name":"CRISPR gene editing","score":0.8069999814033508},{"id":"https://openalex.org/keywords/t-cell-mediated-cytotoxicity","display_name":"T cell cytotoxicity","score":0.7039999961853027},{"id":"https://openalex.org/keywords/t-cell-exhaustion","display_name":"T cell exhaustion","score":0.703000009059906},{"id":"https://openalex.org/keywords/pd-1","display_name":"PD-1","score":0.8930000066757202},{"id":"https://openalex.org/keywords/lag-3","display_name":"LAG-3","score":0.9039999842643738},{"id":"https://openalex.org/keywords/cd69","display_name":"CD69","score":0.8650000095367432},{"id":"https://openalex.org/keywords/4-1bb","display_name":"4-1BB","score":0.9800000190734863},{"id":"https://openalex.org/keywords/tumor-associated-antigens","display_name":"tumor-associated antigens","score":0.6909999847412109},{"id":"https://openalex.org/keywords/adoptive-t-cell-therapy","display_name":"adoptive T cell therapy","score":0.7630000114440918}],"concepts":[{"id":"https://openalex.org/C154317977","wikidata":"https://www.wikidata.org/wiki/Q376266","display_name":"Cytotoxic T cell","level":3,"score":0.5903428792953491},{"id":"https://openalex.org/C73588182","wikidata":"https://www.wikidata.org/wiki/Q2663023","display_name":"Cytolysis","level":4,"score":0.5109345316886902},{"id":"https://openalex.org/C2778227246","wikidata":"https://www.wikidata.org/wiki/Q1365309","display_name":"Glioma","level":2,"score":0.5095024704933167},{"id":"https://openalex.org/C3875195","wikidata":"https://www.wikidata.org/wiki/Q5099363","display_name":"Chimeric antigen receptor","level":4,"score":0.5025100708007812},{"id":"https://openalex.org/C28328180","wikidata":"https://www.wikidata.org/wiki/Q48196","display_name":"Stem cell","level":2,"score":0.5014197826385498},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4644019603729248},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.4519686996936798},{"id":"https://openalex.org/C553184892","wikidata":"https://www.wikidata.org/wiki/Q1141429","display_name":"Flow cytometry","level":2,"score":0.4197060465812683},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.40892040729522705},{"id":"https://openalex.org/C2776090121","wikidata":"https://www.wikidata.org/wiki/Q193529","display_name":"T cell","level":3,"score":0.3983701169490814},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.3744810223579407},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.36589890718460083},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.31941571831703186},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.18493488430976868},{"id":"https://openalex.org/C8891405","wikidata":"https://www.wikidata.org/wiki/Q1059","display_name":"Immune system","level":2,"score":0.1562020182609558},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.09303233027458191}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1158/1538-7445.am2022-2817","doi":"https://doi.org/10.1158/1538-7445.am2022-2817","is_oa":false,"landing_page_url":"https://doi.org/10.1158/1538-7445.am2022-2817","pdf_url":null,"source":{"id":"https://openalex.org/S168522863","display_name":"Cancer Research","issn_l":"0008-5472","issn":["0008-5472","1538-7445"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320273","host_organization_name":"American Association for Cancer Research","host_organization_lineage":["https://openalex.org/P4310320273"],"host_organization_lineage_names":["American Association for Cancer Research"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer Research","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.9537000060081482}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4319433641","https://openalex.org/W3179309620","https://openalex.org/W3112226569","https://openalex.org/W7125677556","https://openalex.org/W7213497846","https://openalex.org/W4322154910","https://openalex.org/W4403520156","https://openalex.org/W4385074674","https://openalex.org/W2899716866","https://openalex.org/W2957974627"],"abstract_inverted_index":{"Abstract":[0],"Glioblastoma":[1],"(GBM)":[2],"is":[3],"an":[4],"aggressive":[5],"brain":[6],"cancer":[7],"without":[8],"effective":[9],"treatments.":[10],"CAR-T":[11,43,80,98,134,155,164,179,214,231,238,258,299],"cells":[12,44,48,81,99,116,135,150,156,180,202,215,232,239,259,300,315],"targeted":[13],"to":[14,28,113,127,162,168],"tumor-associated":[15],"antigens":[16],"offer":[17,260],"promise":[18,263],"for":[19,175,264,323],"treating":[20,265],"GBM.":[21,266],"Here,":[22],"we":[23],"used":[24],"cellular":[25],"impedance":[26,67,75,139],"assays":[27],"compare":[29],"the":[30,72,320],"cytolytic":[31,310],"potency":[32,311],"and":[33,66,108,122,131,183,194,209,223,228,243,251],"kinetics":[34,160],"of":[35,52,105,145,254,319],"conventional":[36],"viral":[37],"vs":[38,177],"non-viral":[39,90],"CRISPR":[40,91,256,306],"engineered":[41,83,301],"GD2":[42,79,97,133,230,257,298],"against":[45,312],"glioma":[46,313],"stem":[47,314],"(GSC),":[49],"a":[50,119],"subpopulation":[51],"glioblastoma":[53],"cells.":[54,165],"Patient-derived":[55],"N08":[56],"GSCs":[57],"were":[58,82,100,111,124],"plated":[59],"at":[60,102,181,197],"50k":[61],"cells/well":[62],"on":[63,71],"96-well":[64],"plates,":[65],"was":[68,172,189],"continuously":[69],"monitored":[70,125],"Maestro":[73],"Z":[74],"platform":[76],"(Axion":[77],"BioSystems).":[78],"using":[84,302],"either":[85],"retroviral":[86,303],"transduction":[87,304],"(RV)":[88],"or":[89,305],"editing":[92,307],"(NV).":[93],"At":[94],"48":[95],"hours,":[96],"added":[101],"Effector:Target":[103],"ratios":[104],"0.1:1,":[106],"1:1,":[107],"10:1.":[109],"Comparisons":[110],"made":[112],"mCherry":[114],"T":[115,142,149,201],"(mCh)":[117],"as":[118,219],"control.":[120],"Impedance":[121],"cytolysis":[123,170],"up":[126],"7":[128,198],"days.":[129,199],"RV":[130,163,178,227],"NV":[132,154,176,213,229,237,255],"caused":[136],"decreases":[137],"in":[138],"consistent":[140],"with":[141,191,236],"cell-mediated":[143],"lysis":[144],"GSCs,":[146,235],"whereas":[147],"mCh":[148],"induced":[151],"little":[152],"change.":[153],"exhibited":[157,203,216],"faster":[158],"killing":[159],"compared":[161],"The":[166,246],"time":[167],"50%":[169],"(KT50)":[171],"significantly":[173],"shorter":[174],"1:1":[182],"10:1":[184],"E:T":[185],"ratios.":[186],"Cytotoxic":[187],"function":[188],"validated":[190],"flow":[192],"cytometry":[193],"cytokine":[195],"analysis":[196],"All":[200],"chronic":[204],"activation":[205],"measured":[206,220],"by":[207,221],"CD69":[208],"CD137":[210],"upregulation.":[211],"Importantly,":[212],"less":[217],"exhaustion,":[218],"PD1":[222],"LAG3":[224],"expression.":[225],"Both":[226],"effectively":[233],"cytolyzed":[234],"exhibiting":[240],"more":[241],"potent":[242],"efficient":[244],"killing.":[245],"high":[247],"potency,":[248],"fast":[249],"kinetics,":[250],"reduced":[252],"exhaustion":[253],"great":[261],"clinical":[262],"Citation":[267],"Format:":[268],"Stacie":[269],"A.":[270],"Chvatal,":[271],"Meghan":[272],"T.":[273],"Logun,":[274],"Denise":[275],"D.":[276],"Sullivan,":[277],"Heather":[278],"B.":[279],"Hayes,":[280],"Daniel":[281,293],"Millard,":[282],"Mueller":[283],"P.":[284],"Katie,":[285],"Nicole":[286],"J.":[287,294],"Piscopo,":[288],"Amritava":[289],"Das,":[290],"Kris":[291],"Saha,":[292],"Brat,":[295],"Lohitash":[296],"Karumbaiah.":[297],"exhibit":[308],"strong":[309],"[abstract].":[316],"In:":[317],"Proceedings":[318],"American":[321],"Association":[322],"Cancer":[324,335],"Research":[325],"Annual":[326],"Meeting":[327],"2022;":[328],"2022":[329],"Apr":[330],"8-13.":[331],"Philadelphia":[332],"(PA):":[333],"AACR;":[334],"Res":[336],"2022;82(12_Suppl):Abstract":[337],"nr":[338],"2817.":[339]},"counts_by_year":[],"updated_date":"2026-10-13T10:08:12.033832","created_date":"2022-06-17T00:00:00"}