{"id":"https://openalex.org/W4282961386","doi":"https://doi.org/10.1158/1538-7445.am2022-sy19-04","title":"Abstract SY19-04: Next-generation macrophage and T cell activation reporters to define functional immune states in solid tumors","display_name":"Abstract SY19-04: Next-generation macrophage and T cell activation reporters to define functional immune states in solid tumors","publication_year":2022,"publication_date":"2022-06-15","ids":{"openalex":"https://openalex.org/W4282961386","doi":"https://doi.org/10.1158/1538-7445.am2022-sy19-04"},"language":"en","primary_location":{"id":"doi:10.1158/1538-7445.am2022-sy19-04","doi":"https://doi.org/10.1158/1538-7445.am2022-sy19-04","is_oa":false,"landing_page_url":"https://doi.org/10.1158/1538-7445.am2022-sy19-04","pdf_url":null,"source":{"id":"https://openalex.org/S168522863","display_name":"Cancer Research","issn_l":"0008-5472","issn":["0008-5472","1538-7445"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320273","host_organization_name":"American Association for Cancer Research","host_organization_lineage":["https://openalex.org/P4310320273"],"host_organization_lineage_names":["American Association for Cancer Research"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer Research","raw_type":"journal-article"},"volume":null,"type":"conference-abstract","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5080786053","display_name":"Arja Ray","orcid":"https://orcid.org/0000-0003-2305-2324","observed_orcids":["https://orcid.org/0000-0003-2305-2324"]},"institutions":[{"id":"https://openalex.org/I180670191","display_name":"University of California, San Francisco","ror":"https://ror.org/043mz5j54","country_code":"US","type":"education","lineage":["https://openalex.org/I180670191"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Arja Ray","raw_affiliation_strings":["1University of California San Francisco, San Francisco, CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1University of California San Francisco, San Francisco, CA","institution_ids":["https://openalex.org/I180670191"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5030109235","display_name":"Kenneth H. Hu","orcid":"https://orcid.org/0000-0002-0294-0202","observed_orcids":["https://orcid.org/0000-0002-0294-0202"]},"institutions":[{"id":"https://openalex.org/I180670191","display_name":"University of California, San Francisco","ror":"https://ror.org/043mz5j54","country_code":"US","type":"education","lineage":["https://openalex.org/I180670191"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kenneth Hu","raw_affiliation_strings":["1University of California San Francisco, San Francisco, CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1University of California San Francisco, San Francisco, CA","institution_ids":["https://openalex.org/I180670191"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5036130616","display_name":"Bushra Samad","orcid":"https://orcid.org/0000-0002-2893-0897","observed_orcids":["https://orcid.org/0000-0002-2893-0897"]},"institutions":[{"id":"https://openalex.org/I180670191","display_name":"University of California, San Francisco","ror":"https://ror.org/043mz5j54","country_code":"US","type":"education","lineage":["https://openalex.org/I180670191"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Bushra Samad","raw_affiliation_strings":["1University of California San Francisco, San Francisco, CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1University of California San Francisco, San Francisco, CA","institution_ids":["https://openalex.org/I180670191"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5010232792","display_name":"Grace A. Hernandez","orcid":"https://orcid.org/0000-0001-6494-6194","observed_orcids":["https://orcid.org/0000-0001-6494-6194"]},"institutions":[{"id":"https://openalex.org/I180670191","display_name":"University of California, San Francisco","ror":"https://ror.org/043mz5j54","country_code":"US","type":"education","lineage":["https://openalex.org/I180670191"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Grace Hernandez","raw_affiliation_strings":["1University of California San Francisco, San Francisco, CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1University of California San Francisco, San Francisco, CA","institution_ids":["https://openalex.org/I180670191"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5018179968","display_name":"Matthew F. Krummel","orcid":"https://orcid.org/0000-0001-7915-3533","observed_orcids":["https://orcid.org/0000-0001-7915-3533"]},"institutions":[{"id":"https://openalex.org/I180670191","display_name":"University of California, San Francisco","ror":"https://ror.org/043mz5j54","country_code":"US","type":"education","lineage":["https://openalex.org/I180670191"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Matthew Krummel","raw_affiliation_strings":["1University of California San Francisco, San Francisco, CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1University of California San Francisco, San Francisco, CA","institution_ids":["https://openalex.org/I180670191"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I180670191"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"82","issue":"12_Supplement","first_page":"SY19","last_page":"04"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10158","display_name":"Cancer Immunotherapy and Biomarkers","score":0.7832000255584717,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10158","display_name":"Cancer Immunotherapy and Biomarkers","score":0.7832000255584717,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10031","display_name":"T-cell and B-cell Immunology","score":0.12540000677108765,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11313","display_name":"Immune cells in cancer","score":0.07150000333786011,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cd69","display_name":"CD69","score":0.7519999742507935},{"id":"https://openalex.org/keywords/t-cell-exhaustion","display_name":"T cell exhaustion","score":0.7870000004768372},{"id":"https://openalex.org/keywords/tumor-microenvironment","display_name":"tumor microenvironment","score":0.6990000009536743},{"id":"https://openalex.org/keywords/tumor-associated-macrophages","display_name":"tumor-associated macrophages","score":0.9750000238418579},{"id":"https://openalex.org/keywords/tam-depletion","display_name":"TAM depletion","score":0.8610000014305115},{"id":"https://openalex.org/keywords/macrophage-polarization","display_name":"macrophage polarization","score":0.7559999823570251},{"id":"https://openalex.org/keywords/t-cell-differentiation-trajectory","display_name":"T cell differentiation trajectory","score":0.4259999990463257},{"id":"https://openalex.org/keywords/effector-cd8-t-cells","display_name":"CD8+ effector T cells","score":0.753000020980835},{"id":"https://openalex.org/keywords/transcriptional-reporter","display_name":"transcriptional reporter","score":0.41999998688697815},{"id":"https://openalex.org/keywords/post-transcriptional-regulation","display_name":"post-transcriptional regulation","score":0.675000011920929},{"id":"https://openalex.org/keywords/immune-checkpoint-blockade","display_name":"immune checkpoint blockade","score":0.32199999690055847},{"id":"https://openalex.org/keywords/cancer-immunotherapy","display_name":"cancer immunotherapy","score":0.41499999165534973}],"concepts":[{"id":"https://openalex.org/C2776090121","wikidata":"https://www.wikidata.org/wiki/Q193529","display_name":"T cell","level":3,"score":0.5908432602882385},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5782637596130371},{"id":"https://openalex.org/C8891405","wikidata":"https://www.wikidata.org/wiki/Q1059","display_name":"Immune system","level":2,"score":0.5574333667755127},{"id":"https://openalex.org/C2776107976","wikidata":"https://www.wikidata.org/wiki/Q1786433","display_name":"Tumor microenvironment","level":3,"score":0.5297471284866333},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5264459848403931},{"id":"https://openalex.org/C51785407","wikidata":"https://www.wikidata.org/wiki/Q2627568","display_name":"Effector","level":2,"score":0.47405192255973816},{"id":"https://openalex.org/C26291073","wikidata":"https://www.wikidata.org/wiki/Q310484","display_name":"Ex vivo","level":3,"score":0.42410099506378174},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.3961915969848633},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.3373821973800659},{"id":"https://openalex.org/C207001950","wikidata":"https://www.wikidata.org/wiki/Q141124","display_name":"In vivo","level":2,"score":0.3268160820007324},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.1685335636138916}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1158/1538-7445.am2022-sy19-04","doi":"https://doi.org/10.1158/1538-7445.am2022-sy19-04","is_oa":false,"landing_page_url":"https://doi.org/10.1158/1538-7445.am2022-sy19-04","pdf_url":null,"source":{"id":"https://openalex.org/S168522863","display_name":"Cancer Research","issn_l":"0008-5472","issn":["0008-5472","1538-7445"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320273","host_organization_name":"American Association for Cancer Research","host_organization_lineage":["https://openalex.org/P4310320273"],"host_organization_lineage_names":["American Association for Cancer Research"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer Research","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.8048999905586243}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4313382607","https://openalex.org/W4412347374","https://openalex.org/W3188249445","https://openalex.org/W1982763703","https://openalex.org/W3083746033","https://openalex.org/W3105860591","https://openalex.org/W4415898781","https://openalex.org/W7202215087","https://openalex.org/W4402679810","https://openalex.org/W2223945343"],"abstract_inverted_index":{"Abstract":[0],"Although":[1],"chronic":[2,195,294],"activation":[3,33,94,122,227,744],"is":[4,19,126,148,183,377,451],"considered":[5],"a":[6,20,118,127,142,156,274,304,350,402,462,469,508,668],"major":[7],"driver":[8],"of":[9,23,78,100,130,141,194,220,237,252,259,278,303,308,312,324,354,360,365,390,411,458,510,526,569,582,632,660,681,686,719,757],"T":[10,28,39,89,120,167,222,247,286,310,325,511,549,573,615,688,742],"cell":[11,90,121,168,171,512,743],"dysfunction":[12,653],"in":[13,45,105,201,262,269,321,329,341,397,431,454,497,524,564,575,584,602,608,622,636,654,664,692,724,751],"the":[14,46,106,133,145,235,250,257,263,266,301,322,388,443,501,516,542,555,565,576,587,594,630,655,658,665,683,703,709,720,758],"tumor":[15,199,203,327,487,543,610,617],"microenvironment":[16],"(TME),":[17],"there":[18],"virtual":[21],"absence":[22],"methodologies":[24],"to":[25,83,108,159,256,289,293,300,393,404,473,482,507,540,558,647,672,695,711,746],"discern":[26],"how":[27],"cells":[29,40,223,248,287,311,317,574,689],"spatiotemporally":[30],"progress":[31],"through":[32],"states":[34,219,241,261,663,750],"into":[35,442],"that":[36,254,356,491,519,534],"dysfunction.":[37],"Furthermore,":[38],"and":[41,86,96,110,189,208,213,245,249,331,339,381,437,475,479,523,567,571,589,598,641,690,702,741],"other":[42],"immune":[43,114,652,662,722,749],"populations":[44],"TME":[47,666],"communicate":[48],"extensively":[49],"with":[50,92,185,336,461,515,644],"tumor-associated":[51],"macrophages":[52,413],"(TAMs)":[53],"which":[54,125,432],"are":[55,64,440,521],"typically":[56],"simply":[57],"described":[58],"as":[59,163,165,370,713,715],"\u2018suppressive\u2019,":[60],"but":[61,545],"whose":[62],"functions":[63],"certainly":[65,379],"more":[66],"complex.":[67],"To":[68,344],"address":[69],"these":[70,112,260,316,603],"critical":[71],"knowledge":[72],"gaps,":[73],"we":[74,154,210,348,677],"designed":[75],"two":[76],"types":[77],"novel":[79,119,428,633],"genetic":[80,352],"marking":[81],"systems":[82],"visualize,":[84],"track":[85],"manipulate":[87,395],"(i)":[88],"subsets":[91,99,597],"distinct":[93,217],"histories":[95],"(ii)":[97],"specific":[98,358],"macrophages,":[101,449],"modulating":[102],"primarily":[103],"those":[104],"TME,":[107,577],"study":[109],"define":[111,696,747],"co-evolving":[113],"states.":[115,699],"We":[116,421,553,627],"generated":[117,155,349],"reporter":[123,129,478,588],"mouse,":[124],"transcriptional":[128,139],"CD69":[131,173],"expressing":[132],"fluorescent":[134],"protein":[135,146],"TFP.":[136],"By":[137,579],"recording":[138],"activity":[140],"locus":[143],"where":[144],"expression":[147,182],"under":[149],"tight,":[150],"activation-dependent":[151],"post-transcriptional":[152],"control,":[153,618],"reporting":[157],"system":[158,472],"read":[160],"out":[161],"contemporary":[162],"well":[164,714],"historic":[166],"triggering.":[169],"While":[170],"surface":[172],"reliably":[174],"indicates":[175],"current":[176],"activation,":[177],"resting":[178],"TFP":[179],"(Cd69":[180],"mRNA)":[181],"downregulated":[184],"successive":[186],"TCR":[187],"triggering":[188],"hence":[190],"an":[191,456,559],"inverse":[192],"marker":[193],"stimulation.":[196,295],"Using":[197],"multiple":[198,485],"models":[200,706],"vivo,":[202,637],"slice":[204],"cultures":[205],"ex":[206],"vivo":[207,585],"scRNA-Seq,":[209,593],"thus":[211,628],"defined":[212],"tracked":[214],"over":[215],"time":[216,639],"functional":[218,313,645,661,748],"intratumoral":[221,309],"based":[224],"on":[225,651],"their":[226,290,297,332,504,620],"histories.":[228],"These":[229,700],"data":[230,531],"revealed":[231,490],"key":[232,595],"insights":[233],"including":[234],"delineation":[236],"quiescent":[238],"versus":[239],"activated":[240,283],"within":[242,448],"progenitor,":[243],"effector":[244,284],"exhausted":[246],"trajectory":[251,272],"inter-conversion":[253],"leads":[255],"emergence":[258],"TME.":[264,656],"Probing":[265],"inflection":[267],"points":[268],"this":[270,346,423,427,459,489],"differentiation":[271],"reveals":[273,533],"particularly":[275],"interesting":[276],"subset":[277,307],"CD69+TFP+":[279],"cells,":[280],"representing":[281],"intratumorally":[282],"CD8":[285,572,687],"prior":[288],"dysfunctional":[291],"adaptation":[292],"Dissecting":[296],"phenotype":[298],"led":[299,506],"discovery":[302],"previously":[305],"unexplored":[306],"significance.":[314],"Indeed,":[315],"were":[318,605],"enriched":[319],"specifically":[320,394,405],"context":[323],"cell-mediated":[326,550,616],"control":[328],"mice":[330,355,635],"phenotypic":[333],"markers":[334],"correlated":[335],"improved":[337],"outcomes":[338],"survival":[340],"human":[342],"patients.":[343],"complement":[345],"system,":[347],"second":[351],"strain":[353],"allowed":[357],"depletion":[359,410,480,536,613],"TAMs.":[361,483],"The":[362],"prevailing":[363],"view":[364],"M1":[366],"vs.":[367,373],"M2":[368],"TAMs":[369,396,492,563,583,691],"ostensibly":[371],"anti-tumor":[372,498,551,624,693],"pro-tumor,":[374],"although":[375],"useful,":[376],"almost":[378],"over-simplistic":[380],"has":[382],"lacked":[383],"further":[384],"delineation,":[385],"limited":[386],"by":[387,425],"quality":[389],"available":[391],"tools":[392],"situ.":[398],"Remarkably,":[399],"till":[400],"now,":[401],"method":[403],"deplete":[406],"TAMs,":[407],"without":[408],"global":[409,527],"all":[412],"(e.g":[414],"CSF1R":[415],"blockade,":[416],"Clodronate)":[417],"did":[418],"not":[419,537],"exist.":[420],"alleviated":[422],"problem":[424],"using":[426,586],"mouse":[429],"strain,":[430],"conditional":[433],"(lox-Stop-lox":[434],"flanked)":[435],"YFP":[436],"DTR":[438],"genes":[439],"knocked":[441],"endogenous":[444],"CD206":[445,450],"locus.":[446],"Since,":[447],"largely":[452],"expressed":[453],"tumors,":[455],"intercross":[457],"allele":[460],"Csf1r-Cre":[463],"(to":[464],"specify":[465],"monocyte-derived":[466],"lineage)":[467],"provides":[468,667],"2-allele":[470],"gating":[471],"limit":[474],"robustly":[476],"penetrate":[477],"function":[481],"In":[484],"TAM-rich":[486],"models,":[488,611],"assumed":[493],"context-dependent":[494],"divergent":[495],"roles":[496],"immunity.":[499,552,626],"On":[500],"one":[502],"hand,":[503],"removal":[505],"mitigation":[509],"exhaustion,":[513],"consistent":[514],"popular":[517],"notion":[518],"they":[520],"immunosuppressive":[522],"need":[525],"elimination.":[528],"However,":[529],"our":[530,717],"also":[532,546],"macrophage":[535,740],"only":[538],"fails":[539],"alleviate":[541],"burden":[544],"prevents":[547],"successful":[548],"linked":[554],"latter":[556],"effect":[557],"obligate":[560],"role":[561],"for":[562,761],"recruitment":[566],"maintenance":[568],"cDC1s":[570],"respectively.":[578],"live":[580],"imaging":[581,640],"subsequent":[590],"spatially":[591],"resolved":[592],"TAM":[596,612],"interactional":[599],"networks":[600],"involved":[601],"processes":[604],"established.":[606],"Importantly,":[607],"responsive":[609],"subverted":[614],"demonstrating":[619],"importance":[621],"organizing":[623],"adaptive":[625],"combine":[629],"use":[631],"engineered":[634],"real":[638],"spatial":[642],"transcriptomics":[643],"studies":[646],"shed":[648],"new":[649],"light":[650],"Defining":[657],"ontology":[659],"clear":[669],"target":[670],"space":[671],"maneuver":[673],"cancer":[674],"immunotherapies.":[675],"Herein,":[676],"offer":[678],"prime":[679],"examples":[680],"dissecting":[682],"spatiotemporal":[684],"biology":[685],"immunity":[694],"such":[697],"interdependent":[698],"findings":[701],"newly":[704],"established":[705],"therein":[707],"pave":[708],"way":[710],"deepen":[712],"broaden":[716],"understanding":[718],"complex":[721],"landscape":[723],"solid":[725,752],"tumors.":[726],"Citation":[727],"Format:":[728],"Arja":[729],"Ray,":[730],"Kenneth":[731],"Hu,":[732],"Bushra":[733],"Samad,":[734],"Grace":[735],"Hernandez,":[736],"Matthew":[737],"Krummel.":[738],"Next-generation":[739],"reporters":[745],"tumors":[753],"[abstract].":[754],"In:":[755],"Proceedings":[756],"American":[759],"Association":[760],"Cancer":[762,773],"Research":[763],"Annual":[764],"Meeting":[765],"2022;":[766],"2022":[767],"Apr":[768],"8-13.":[769],"Philadelphia":[770],"(PA):":[771],"AACR;":[772],"Res":[774],"2022;82(12_Suppl):Abstract":[775],"nr":[776],"SY19-04.":[777]},"counts_by_year":[],"updated_date":"2026-07-14T23:27:15.235271","created_date":"2022-06-17T00:00:00"}