{"id":"https://openalex.org/W4282945526","doi":"https://doi.org/10.1158/1538-7445.am2022-1474","title":"Abstract 1474: Genome edited colorectal cancer organoid models reveal distinct microRNA activity patterns across different mutation profiles","display_name":"Abstract 1474: Genome edited colorectal cancer organoid models reveal distinct microRNA activity patterns across different mutation profiles","publication_year":2022,"publication_date":"2022-06-15","ids":{"openalex":"https://openalex.org/W4282945526","doi":"https://doi.org/10.1158/1538-7445.am2022-1474"},"language":"en","primary_location":{"id":"doi:10.1158/1538-7445.am2022-1474","doi":"https://doi.org/10.1158/1538-7445.am2022-1474","is_oa":false,"landing_page_url":"https://doi.org/10.1158/1538-7445.am2022-1474","pdf_url":null,"source":{"id":"https://openalex.org/S168522863","display_name":"Cancer Research","issn_l":"0008-5472","issn":["0008-5472","1538-7445"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320273","host_organization_name":"American Association for Cancer Research","host_organization_lineage":["https://openalex.org/P4310320273"],"host_organization_lineage_names":["American Association for Cancer Research"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer Research","raw_type":"journal-article"},"volume":null,"type":"conference-abstract","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5053197795","display_name":"Jonathan Villanueva","orcid":"https://orcid.org/0000-0002-1789-6822","observed_orcids":["https://orcid.org/0000-0002-1789-6822"]},"institutions":[{"id":"https://openalex.org/I205783295","display_name":"Cornell University","ror":"https://ror.org/05bnh6r87","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jonathan W. Villanueva","raw_affiliation_strings":["1Cornell University, Ithaca, NY;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Cornell University, Ithaca, NY;","institution_ids":["https://openalex.org/I205783295"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5040776645","display_name":"Lawrence N. Kwong","orcid":"https://orcid.org/0000-0001-8370-1432","observed_orcids":["https://orcid.org/0000-0001-8370-1432"]},"institutions":[{"id":"https://openalex.org/I205783295","display_name":"Cornell University","ror":"https://ror.org/05bnh6r87","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Lawrence Kwong","raw_affiliation_strings":["1Cornell University, Ithaca, NY;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Cornell University, Ithaca, NY;","institution_ids":["https://openalex.org/I205783295"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Teng Han","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4387153466","display_name":"Weill Cornell Medicine","ror":"https://ror.org/02r109517","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295","https://openalex.org/I4387153466"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Teng Han","raw_affiliation_strings":["2Weill Cornell Medicine, New York City, NY"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"2Weill Cornell Medicine, New York City, NY","institution_ids":["https://openalex.org/I4387153466"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5060336621","display_name":"Salvador Alonso Martinez","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4387153466","display_name":"Weill Cornell Medicine","ror":"https://ror.org/02r109517","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295","https://openalex.org/I4387153466"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Salvador Alonso Martinez","raw_affiliation_strings":["2Weill Cornell Medicine, New York City, NY"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"2Weill Cornell Medicine, New York City, NY","institution_ids":["https://openalex.org/I4387153466"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5030066005","display_name":"Fong Cheng Pan","orcid":"https://orcid.org/0000-0002-9393-6574","observed_orcids":["https://orcid.org/0000-0002-9393-6574"]},"institutions":[{"id":"https://openalex.org/I4387153466","display_name":"Weill Cornell Medicine","ror":"https://ror.org/02r109517","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295","https://openalex.org/I4387153466"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Fong Cheng Pan","raw_affiliation_strings":["2Weill Cornell Medicine, New York City, NY"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"2Weill Cornell Medicine, New York City, NY","institution_ids":["https://openalex.org/I4387153466"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102892780","display_name":"Michael T. Shanahan","orcid":"https://orcid.org/0000-0002-8411-4050","observed_orcids":["https://orcid.org/0000-0002-8411-4050","https://orcid.org/0000-0003-4650-5787"]},"institutions":[{"id":"https://openalex.org/I205783295","display_name":"Cornell University","ror":"https://ror.org/05bnh6r87","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Michael T. Shanahan","raw_affiliation_strings":["1Cornell University, Ithaca, NY;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Cornell University, Ithaca, NY;","institution_ids":["https://openalex.org/I205783295"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5002870194","display_name":"Matt Kanke","orcid":"https://orcid.org/0000-0002-7227-3054","observed_orcids":["https://orcid.org/0000-0002-7227-3054"]},"institutions":[{"id":"https://openalex.org/I205783295","display_name":"Cornell University","ror":"https://ror.org/05bnh6r87","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Matt Kanke","raw_affiliation_strings":["1Cornell University, Ithaca, NY;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Cornell University, Ithaca, NY;","institution_ids":["https://openalex.org/I205783295"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5024015568","display_name":"Shuibing Chen","orcid":"https://orcid.org/0000-0002-6294-5187","observed_orcids":["https://orcid.org/0000-0002-6294-5187"]},"institutions":[{"id":"https://openalex.org/I4387153466","display_name":"Weill Cornell Medicine","ror":"https://ror.org/02r109517","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295","https://openalex.org/I4387153466"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Shuibing Chen","raw_affiliation_strings":["2Weill Cornell Medicine, New York City, NY"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"2Weill Cornell Medicine, New York City, NY","institution_ids":["https://openalex.org/I4387153466"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5021011616","display_name":"Lukas E. Dow","orcid":"https://orcid.org/0000-0001-7048-1418","observed_orcids":["https://orcid.org/0000-0001-7048-1418"]},"institutions":[{"id":"https://openalex.org/I4387153466","display_name":"Weill Cornell Medicine","ror":"https://ror.org/02r109517","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295","https://openalex.org/I4387153466"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Lukas E. Dow","raw_affiliation_strings":["2Weill Cornell Medicine, New York City, NY"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"2Weill Cornell Medicine, New York City, NY","institution_ids":["https://openalex.org/I4387153466"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5024923108","display_name":"Charles G. Danko","orcid":"https://orcid.org/0000-0002-1999-7125","observed_orcids":["https://orcid.org/0000-0002-1999-7125"]},"institutions":[{"id":"https://openalex.org/I205783295","display_name":"Cornell University","ror":"https://ror.org/05bnh6r87","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Charles G. Danko","raw_affiliation_strings":["1Cornell University, Ithaca, NY;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Cornell University, Ithaca, NY;","institution_ids":["https://openalex.org/I205783295"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5067207319","display_name":"Praveen Sethupathy","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I205783295","display_name":"Cornell University","ror":"https://ror.org/05bnh6r87","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Praveen Sethupathy","raw_affiliation_strings":["1Cornell University, Ithaca, NY;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Cornell University, Ithaca, NY;","institution_ids":["https://openalex.org/I205783295"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"82","issue":"12_Supplement","first_page":"1474","last_page":"1474"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10062","display_name":"MicroRNA in disease regulation","score":0.9965999722480774,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10062","display_name":"MicroRNA in disease regulation","score":0.9965999722480774,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11369","display_name":"Genetic factors in colorectal cancer","score":0.0020000000949949026,"subfield":{"id":"https://openalex.org/subfields/2734","display_name":"Pathology and Forensic Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10515","display_name":"Cancer-related molecular mechanisms research","score":0.00039999998989515007,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/colorectal-cancer","display_name":"colorectal cancer","score":0.9990000128746033},{"id":"https://openalex.org/keywords/microrna","display_name":"microRNA","score":0.7620000243186951},{"id":"https://openalex.org/keywords/mir-24-3p","display_name":"miR-24-3p","score":0.9990000128746033},{"id":"https://openalex.org/keywords/intestinal-organoids","display_name":"intestinal organoids","score":0.6499999761581421},{"id":"https://openalex.org/keywords/enteroids","display_name":"enteroids","score":0.7850000262260437},{"id":"https://openalex.org/keywords/oncogenic-mutations","display_name":"oncogenic mutations","score":0.7630000114440918},{"id":"https://openalex.org/keywords/gene-regulatory-networks","display_name":"gene regulatory networks","score":0.6029999852180481},{"id":"https://openalex.org/keywords/apoptosis","display_name":"apoptosis","score":0.9480000138282776},{"id":"https://openalex.org/keywords/heme-oxygenase-1","display_name":"heme oxygenase-1","score":0.9100000262260437},{"id":"https://openalex.org/keywords/prss8","display_name":"PRSS8","score":0.9990000128746033},{"id":"https://openalex.org/keywords/precision-medicine","display_name":"precision medicine","score":0.6549999713897705},{"id":"https://openalex.org/keywords/tumor-cell-survival","display_name":"tumor cell survival","score":0.5600000023841858},{"id":"https://openalex.org/keywords/hct116-cells","display_name":"HCT116 cells","score":0.9470000267028809},{"id":"https://openalex.org/keywords/microrna-target-genes","display_name":"miRNA target genes","score":0.41100001335144043}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8015395402908325},{"id":"https://openalex.org/C145059251","wikidata":"https://www.wikidata.org/wiki/Q310899","display_name":"microRNA","level":3,"score":0.7207974195480347},{"id":"https://openalex.org/C526805850","wikidata":"https://www.wikidata.org/wiki/Q188874","display_name":"Colorectal cancer","level":3,"score":0.5233851671218872},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.521513044834137},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.5201390385627747},{"id":"https://openalex.org/C121608353","wikidata":"https://www.wikidata.org/wiki/Q12078","display_name":"Cancer","level":2,"score":0.48202693462371826},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.4647570848464966},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.44263580441474915},{"id":"https://openalex.org/C6929976","wikidata":"https://www.wikidata.org/wiki/Q3771881","display_name":"Regulator","level":3,"score":0.42632579803466797},{"id":"https://openalex.org/C165864922","wikidata":"https://www.wikidata.org/wiki/Q411391","display_name":"Regulation of gene expression","level":3,"score":0.4127143621444702}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1158/1538-7445.am2022-1474","doi":"https://doi.org/10.1158/1538-7445.am2022-1474","is_oa":false,"landing_page_url":"https://doi.org/10.1158/1538-7445.am2022-1474","pdf_url":null,"source":{"id":"https://openalex.org/S168522863","display_name":"Cancer Research","issn_l":"0008-5472","issn":["0008-5472","1538-7445"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320273","host_organization_name":"American Association for Cancer Research","host_organization_lineage":["https://openalex.org/P4310320273"],"host_organization_lineage_names":["American Association for Cancer Research"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer Research","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.9476000070571899,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4200497485","https://openalex.org/W4310709067","https://openalex.org/W6940402757","https://openalex.org/W4385386960","https://openalex.org/W2031621381","https://openalex.org/W2319420238","https://openalex.org/W2048532811","https://openalex.org/W2509884289","https://openalex.org/W2947075578","https://openalex.org/W1981055638"],"abstract_inverted_index":{"Abstract":[0],"Colorectal":[1],"cancer":[2,9,342],"(CRC)":[3],"is":[4,69,82,173,204,281],"the":[5,92,213,221,256,358],"third":[6],"most":[7],"common":[8],"and":[10,33,67,94,127,266,269,291],"second":[11],"leading":[12,49],"cause":[13],"of":[14,37,62,87,96,116,136,149,158,178,197,223,258,285,357],"cancer-related":[15],"deaths":[16],"worldwide.":[17],"DNA":[18],"mutations":[19,38,89,138,199],"drive":[20],"colon":[21],"tumor":[22,286],"formation":[23],"by":[24,77,228],"disrupting":[25],"gene":[26,46,63,252],"regulatory":[27,47],"mechanisms":[28,48],"that":[29,71,100,139,160,171,180,202,218,243,254,279],"facilitate":[30],"cell":[31,215,247,287],"survival":[32],"growth.":[34],"Distinct":[35],"combinations":[36,86,135,196],"can":[39,74],"result":[40],"in":[41,52,65,108,142,163,183,189,212,239,289,307],"unique":[42],"changes":[43,107],"to":[44,50,54],"these":[45],"variability":[51],"responses":[53],"available":[55],"therapeutics.":[56],"MicroRNAs":[57],"(miRNAs)":[58],"are":[59,161,181],"important":[60],"regulators":[61],"expression":[64,73,95],"CRC":[66,109,290],"it":[68,81],"known":[70],"their":[72],"be":[75,113],"altered":[76,114],"oncogenic":[78,137,198],"mutations.":[79],"However,":[80],"unknown":[83],"how":[84],"distinct":[85,147,346],"CRC-risk":[88],"differentially":[90],"affect":[91],"regulation":[93],"miRNAs.":[97],"We":[98,153],"hypothesize":[99],"some":[101],"miRNAs":[102,159],"will":[103,112],"exhibit":[104],"strong":[105],"genotype-dependent":[106,150],"whereas":[110],"others":[111],"irrespective":[115],"genotype.":[117],"Utilizing":[118],"sequencing":[119],"data":[120],"from":[121],"The":[122],"Cancer":[123,362,373],"Genome":[124,339],"Atlas":[125],"(TCGA)":[126],"cutting-edge":[128],"intestinal":[129,246],"organoid":[130,343],"(enteroid)":[131],"models":[132,344],"with":[133,194],"different":[134,195,351],"commonly":[140],"occur":[141],"CRC,":[143],"we":[144,169],"identified":[145,267],"several":[146],"patterns":[148,349],"miRNA":[151],"expression.":[152],"also":[154],"found":[155],"a":[156,164,174,282,299],"suite":[157],"up-regulated":[162],"mutation-independent":[165],"manner.":[166],"Among":[167],"these,":[168],"demonstrated":[170],"miR-24-3p":[172,188,203,219,274,280],"candidate":[175,251,273],"master":[176],"regulator":[177,284],"genes":[179],"down-regulated":[182],"all":[184],"mutational-contexts.":[185],"RT-qPCR":[186],"for":[187,304,361],"genetically":[190],"modified":[191],"human":[192],"colonoids":[193],"further":[200,293],"supports":[201],"elevated":[205],"across":[206,350],"various":[207],"mutational":[208],"contexts.":[209],"Functional":[210],"studies":[211,238],"HCT116":[214],"line":[216],"revealed":[217],"controls":[220,245],"number":[222],"metabolically":[224],"active,":[225],"viable":[226],"cells":[227],"regulating":[229],"apoptosis":[230,288],"but":[231],"not":[232],"proliferation.":[233],"Moreover,":[234],"follow-up":[235],"ex":[236],"vivo":[237],"mouse":[240],"enteroids":[241],"confirmed":[242],"miR-24":[244],"survival.":[248],"To":[249],"identify":[250],"targets":[253],"mediate":[255],"function":[257],"miR-24-3p,":[259],"I":[260],"performed":[261],"an":[262],"RNA-sequencing":[263],"(RNA-seq)":[264],"analysis":[265],"HMOX1":[268],"PRSS8":[270],"as":[271],"top":[272],"targets.":[275],"These":[276],"findings":[277,297],"suggest":[278],"genotype-independent":[283],"merits":[292],"investigation.":[294],"Overall,":[295],"our":[296],"provide":[298],"novel":[300],"perspective":[301],"on":[302],"approaches":[303],"precision":[305],"medicine":[306],"CRC.":[308],"Citation":[309],"Format:":[310],"Jonathan":[311],"W.":[312],"Villanueva,":[313],"Lawrence":[314],"Kwong,":[315],"Teng":[316],"Han,":[317],"Salvador":[318],"Alonso":[319],"Martinez,":[320],"Fong":[321],"Cheng":[322],"Pan,":[323],"Michael":[324],"T.":[325],"Shanahan,":[326],"Matt":[327],"Kanke,":[328],"Shuibing":[329],"Chen,":[330],"Lukas":[331],"E.":[332],"Dow,":[333],"Charles":[334],"G.":[335],"Danko,":[336],"Praveen":[337],"Sethupathy.":[338],"edited":[340],"colorectal":[341],"reveal":[345],"microRNA":[347],"activity":[348],"mutation":[352],"profiles":[353],"[abstract].":[354],"In:":[355],"Proceedings":[356],"American":[359],"Association":[360],"Research":[363],"Annual":[364],"Meeting":[365],"2022;":[366],"2022":[367],"Apr":[368],"8-13.":[369],"Philadelphia":[370],"(PA):":[371],"AACR;":[372],"Res":[374],"2022;82(12_Suppl):Abstract":[375],"nr":[376],"1474.":[377]},"counts_by_year":[],"updated_date":"2026-09-29T14:09:16.559479","created_date":"2022-06-16T00:00:00"}
