{"id":"https://openalex.org/W4282937482","doi":"https://doi.org/10.1021/acsbiomaterials.2c00125","title":"Covalent Labeling-Mass Spectrometry Provides a Molecular Understanding of Noncovalent Polymer\u2013Protein Complexation","display_name":"Covalent Labeling-Mass Spectrometry Provides a Molecular Understanding of Noncovalent Polymer\u2013Protein Complexation","publication_year":2022,"publication_date":"2022-05-24","ids":{"openalex":"https://openalex.org/W4282937482","doi":"https://doi.org/10.1021/acsbiomaterials.2c00125","pmid":"https://pubmed.ncbi.nlm.nih.gov/35608244","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/9205173"},"language":"en","primary_location":{"id":"doi:10.1021/acsbiomaterials.2c00125","doi":"https://doi.org/10.1021/acsbiomaterials.2c00125","is_oa":false,"landing_page_url":"https://doi.org/10.1021/acsbiomaterials.2c00125","pdf_url":null,"source":{"id":"https://openalex.org/S2764739176","display_name":"ACS Biomaterials Science & Engineering","issn_l":"2373-9878","issn":["2373-9878"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ACS Biomaterials Science & Engineering","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/9205173","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5078067399","display_name":"Hazel C. Davis","orcid":"https://orcid.org/0000-0003-3675-1650","observed_orcids":["https://orcid.org/0000-0003-3675-1650"]},"institutions":[{"id":"https://openalex.org/I24603500","display_name":"University of Massachusetts Amherst","ror":"https://ror.org/0072zz521","country_code":"US","type":"education","lineage":["https://openalex.org/I24603500"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Hazel C. Davis","raw_affiliation_strings":["Department of Polymer Science and Engineering, University of Massachusetts Amherst, Amherst, Massachusetts 01003, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Polymer Science and Engineering, University of Massachusetts Amherst, Amherst, Massachusetts 01003, United States","institution_ids":["https://openalex.org/I24603500"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102014181","display_name":"Xiao Pan","orcid":"https://orcid.org/0000-0002-4444-8275","observed_orcids":["https://orcid.org/0000-0002-4444-8275"]},"institutions":[{"id":"https://openalex.org/I24603500","display_name":"University of Massachusetts Amherst","ror":"https://ror.org/0072zz521","country_code":"US","type":"education","lineage":["https://openalex.org/I24603500"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Xiao Pan","raw_affiliation_strings":["Department of Chemistry, University of Massachusetts Amherst, Amherst, Massachusetts 01003, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of Massachusetts Amherst, Amherst, Massachusetts 01003, United States","institution_ids":["https://openalex.org/I24603500"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5054505493","display_name":"Zachary J. Kirsch","orcid":"https://orcid.org/0000-0001-7788-6223","observed_orcids":["https://orcid.org/0000-0001-7788-6223"]},"institutions":[{"id":"https://openalex.org/I24603500","display_name":"University of Massachusetts Amherst","ror":"https://ror.org/0072zz521","country_code":"US","type":"education","lineage":["https://openalex.org/I24603500"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Zachary J. Kirsch","raw_affiliation_strings":["Department of Chemistry, University of Massachusetts Amherst, Amherst, Massachusetts 01003, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of Massachusetts Amherst, Amherst, Massachusetts 01003, United States","institution_ids":["https://openalex.org/I24603500"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5070876073","display_name":"Richard W. Vachet","orcid":"https://orcid.org/0000-0003-4514-0210","observed_orcids":["https://orcid.org/0000-0003-4514-0210"]},"institutions":[{"id":"https://openalex.org/I24603500","display_name":"University of Massachusetts Amherst","ror":"https://ror.org/0072zz521","country_code":"US","type":"education","lineage":["https://openalex.org/I24603500"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Richard W. Vachet","raw_affiliation_strings":["Department of Chemistry, University of Massachusetts Amherst, Amherst, Massachusetts 01003, United States"],"raw_orcid":"https://orcid.org/0000-0003-4514-0210","affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of Massachusetts Amherst, Amherst, Massachusetts 01003, United States","institution_ids":["https://openalex.org/I24603500"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5086363084","display_name":"Gregory N. Tew","orcid":"https://orcid.org/0000-0003-3277-7925","observed_orcids":["https://orcid.org/0000-0003-3277-7925"]},"institutions":[{"id":"https://openalex.org/I24603500","display_name":"University of Massachusetts Amherst","ror":"https://ror.org/0072zz521","country_code":"US","type":"education","lineage":["https://openalex.org/I24603500"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Gregory N. Tew","raw_affiliation_strings":["Department of Polymer Science and Engineering, University of Massachusetts Amherst, Amherst, Massachusetts 01003, United States"],"raw_orcid":"https://orcid.org/0000-0003-3277-7925","affiliations":[{"raw_affiliation_string":"Department of Polymer Science and Engineering, University of Massachusetts Amherst, Amherst, Massachusetts 01003, United States","institution_ids":["https://openalex.org/I24603500"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5070876073","https://openalex.org/A5086363084"],"corresponding_institution_ids":["https://openalex.org/I24603500"],"apc_list":{"value":5000,"currency":"USD","value_usd":5000},"apc_paid":null,"fwci":0.1805,"has_fulltext":false,"cited_by_count":2,"citation_normalized_percentile":{"value":0.28298312,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"8","issue":"6","first_page":"2489","last_page":"2499"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.4431000053882599,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.4431000053882599,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10063","display_name":"Nanoparticle-Based Drug Delivery","score":0.21459999680519104,"subfield":{"id":"https://openalex.org/subfields/2502","display_name":"Biomaterials"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10405","display_name":"Advanced Polymer Synthesis and Characterization","score":0.0828000009059906,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/protein-delivery","display_name":"protein delivery","score":0.7509999871253967},{"id":"https://openalex.org/keywords/amphiphilic-block-copolymers","display_name":"amphiphilic block copolymers","score":0.8880000114440918},{"id":"https://openalex.org/keywords/sfgfp","display_name":"sfGFP","score":0.9089999794960022},{"id":"https://openalex.org/keywords/electrostatic-interactions","display_name":"electrostatic interactions","score":0.8019999861717224},{"id":"https://openalex.org/keywords/hydrophobic-interactions","display_name":"hydrophobic interactions","score":0.9769999980926514},{"id":"https://openalex.org/keywords/binding-site-mapping","display_name":"binding site mapping","score":0.5210000276565552}],"concepts":[{"id":"https://openalex.org/C137277065","wikidata":"https://www.wikidata.org/wiki/Q2915446","display_name":"Non-covalent interactions","level":4,"score":0.7521722316741943},{"id":"https://openalex.org/C180577832","wikidata":"https://www.wikidata.org/wiki/Q127920","display_name":"Covalent bond","level":2,"score":0.6996385455131531},{"id":"https://openalex.org/C162356407","wikidata":"https://www.wikidata.org/wiki/Q180809","display_name":"Mass spectrometry","level":2,"score":0.6915007829666138},{"id":"https://openalex.org/C521977710","wikidata":"https://www.wikidata.org/wiki/Q81163","display_name":"Polymer","level":2,"score":0.5832381248474121},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5319657325744629},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.36230969429016113},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.30924004316329956},{"id":"https://openalex.org/C32909587","wikidata":"https://www.wikidata.org/wiki/Q11369","display_name":"Molecule","level":2,"score":0.2513911724090576},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.199346661567688},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.17208704352378845},{"id":"https://openalex.org/C112887158","wikidata":"https://www.wikidata.org/wiki/Q169324","display_name":"Hydrogen bond","level":3,"score":0.12876233458518982}],"mesh":[{"descriptor_ui":"D011108","descriptor_name":"Polymers","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D013058","descriptor_name":"Mass Spectrometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D057927","descriptor_name":"Hydrophobic and Hydrophilic Interactions","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1021/acsbiomaterials.2c00125","doi":"https://doi.org/10.1021/acsbiomaterials.2c00125","is_oa":false,"landing_page_url":"https://doi.org/10.1021/acsbiomaterials.2c00125","pdf_url":null,"source":{"id":"https://openalex.org/S2764739176","display_name":"ACS Biomaterials Science & Engineering","issn_l":"2373-9878","issn":["2373-9878"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ACS Biomaterials Science & Engineering","raw_type":"journal-article"},{"id":"pmid:35608244","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/35608244","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ACS biomaterials science & engineering","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:9205173","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/9205173","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"ACS Biomater Sci Eng","raw_type":"Text"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:9205173","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/9205173","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"ACS Biomater Sci Eng","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G3403323728","display_name":"Acquisition of an Orbitrap MS","funder_award_id":"1s10od010645-01a1","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G4472611239","display_name":"Copper-Induced Amyloidosis Studied by Mass Spectrometry","funder_award_id":"R01GM075092","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320332749","display_name":"University of Massachusetts Amherst","ror":"https://ror.org/0072zz521"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":47,"referenced_works":["https://openalex.org/W83613284","https://openalex.org/W653337582","https://openalex.org/W1487420490","https://openalex.org/W1981183233","https://openalex.org/W1983995089","https://openalex.org/W2010959934","https://openalex.org/W2018272940","https://openalex.org/W2018569753","https://openalex.org/W2025938842","https://openalex.org/W2026507477","https://openalex.org/W2026582002","https://openalex.org/W2048023847","https://openalex.org/W2080210438","https://openalex.org/W2103647536","https://openalex.org/W2107107651","https://openalex.org/W2123670081","https://openalex.org/W2145706118","https://openalex.org/W2160238947","https://openalex.org/W2161574105","https://openalex.org/W2169515696","https://openalex.org/W2170036411","https://openalex.org/W2261220794","https://openalex.org/W2285099499","https://openalex.org/W2321933331","https://openalex.org/W2410022430","https://openalex.org/W2498346280","https://openalex.org/W2549105095","https://openalex.org/W2550421694","https://openalex.org/W2586145470","https://openalex.org/W2600588343","https://openalex.org/W2795922219","https://openalex.org/W2886335353","https://openalex.org/W2890792159","https://openalex.org/W2909156763","https://openalex.org/W2913036494","https://openalex.org/W2947874091","https://openalex.org/W2956368132","https://openalex.org/W2969560376","https://openalex.org/W2986779843","https://openalex.org/W2994778055","https://openalex.org/W3000838175","https://openalex.org/W3016573515","https://openalex.org/W3027737160","https://openalex.org/W3121015296","https://openalex.org/W3173141729","https://openalex.org/W3173464281","https://openalex.org/W4200252967"],"related_works":["https://openalex.org/W2889517691","https://openalex.org/W3013493982","https://openalex.org/W6902728149","https://openalex.org/W3095464944","https://openalex.org/W3033139795","https://openalex.org/W1969096480","https://openalex.org/W3014823084","https://openalex.org/W2063559309","https://openalex.org/W2795922219","https://openalex.org/W6959135523"],"abstract_inverted_index":{"The":[0],"delivery":[1,55,203,235],"of":[2,15,24,54,56,66,70,76,86,105,113,123,141,146,158,190,199,209],"functional":[3,57],"proteins":[4],"to":[5,128,151,180,192,222],"the":[6,13,22,30,52,67,103,118,121,139,184,188,197,210],"intracellular":[7],"space":[8],"offers":[9],"tremendous":[10],"advantages":[11],"for":[12,117,143,233],"development":[14],"new":[16,106],"therapeutics":[17],"but":[18,109],"is":[19,37],"limited":[20],"by":[21,39],"passage":[23],"these":[25],"large":[26],"polar":[27],"biomacromolecules":[28],"through":[29],"cell":[31],"membrane.":[32],"Noncovalent":[33],"polymer-protein":[34,88,135,214],"binding":[35,78,145,215],"that":[36],"driven":[38],"strong":[40],"carrier-cargo":[41],"interactions,":[42],"including":[43,90],"electrostatics":[44],"and":[45,97,154,216],"hydrophobicity,":[46],"has":[47],"previously":[48],"been":[49],"explored":[50],"in":[51,102,196,213],"context":[53,198],"proteins.":[58],"Appropriately":[59],"designed":[60,232],"polymer-based":[61,107],"carriers":[62,108,227],"can":[63,82,228],"take":[64],"advantage":[65],"heterogeneous":[68],"surface":[69,131,156,186],"protein":[71,165,193,202,223,234],"cargoes,":[72],"where":[73,219],"multiple":[74],"types":[75],"physical":[77],"interactions":[79,132,212],"with":[80],"polymers":[81,220],"occur.":[83],"Traditional":[84],"methods":[85],"assessing":[87],"binding,":[89],"dynamic":[91],"light":[92],"scattering,":[93],"circular":[94],"dichroism":[95],"spectroscopy,":[96],"fluorescence-based":[98],"assays,":[99],"are":[100],"useful":[101],"study":[104],"face":[110],"a":[111,159,170],"number":[112],"limitations.":[114],"We":[115,137],"implement":[116],"first":[119],"time":[120],"method":[122,179],"covalent":[124],"labeling-mass":[125],"spectrometry":[126],"(CL-MS)":[127],"probe":[129],"intermolecular":[130,185,211],"within":[133],"noncovalent":[134,225],"complexes.":[136],"demonstrate":[138],"utility":[140],"CL-MS":[142],"establishing":[144],"an":[147,207],"amphiphilic":[148],"block":[149],"copolymer":[150],"negatively":[152],"charged":[153],"hydrophobic":[155],"patches":[157],"model":[160],"protein,":[161],"superfolder":[162],"green":[163],"fluorescent":[164],"(sfGFP),":[166],"using":[167],"diethylpyrocarbonate":[168],"as":[169,187],"pseudo-specific":[171],"labeling":[172],"reagent.":[173],"In":[174],"addition,":[175],"we":[176],"utilize":[177],"this":[178],"explore":[181],"differences":[182],"at":[183],"ratio":[189],"polymer":[191,226],"increases,":[194],"particularly":[195],"defining":[200],"effective":[201],"regimes.":[204],"By":[205],"promoting":[206],"understanding":[208],"identifying":[217],"sites":[218],"bind":[221],"surfaces,":[224],"be":[229],"more":[230],"effectively":[231],"applications.":[236]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2022-06-16T00:00:00"}