{"id":"https://openalex.org/W4282919443","doi":"https://doi.org/10.1158/1538-7445.am2022-3286","title":"Abstract 3286: Development and characterization of an oncolytic adenovirus armed with the immune checkpoint stimulator SA-4-1BBL","display_name":"Abstract 3286: Development and characterization of an oncolytic adenovirus armed with the immune checkpoint stimulator SA-4-1BBL","publication_year":2022,"publication_date":"2022-06-15","ids":{"openalex":"https://openalex.org/W4282919443","doi":"https://doi.org/10.1158/1538-7445.am2022-3286"},"language":"en","primary_location":{"id":"doi:10.1158/1538-7445.am2022-3286","doi":"https://doi.org/10.1158/1538-7445.am2022-3286","is_oa":false,"landing_page_url":"https://doi.org/10.1158/1538-7445.am2022-3286","pdf_url":null,"source":{"id":"https://openalex.org/S168522863","display_name":"Cancer Research","issn_l":"0008-5472","issn":["0008-5472","1538-7445"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320273","host_organization_name":"American Association for Cancer Research","host_organization_lineage":["https://openalex.org/P4310320273"],"host_organization_lineage_names":["American Association for Cancer Research"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer Research","raw_type":"journal-article"},"volume":null,"type":"conference-abstract","is_traditional":false,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5077647701","display_name":"Jorge G. G\u00f3mez-Guti\u00e9rrez","orcid":"https://orcid.org/0000-0003-3610-260X","observed_orcids":["https://orcid.org/0000-0003-3610-260X"]},"institutions":[{"id":"https://openalex.org/I76835614","display_name":"University of Missouri","ror":"https://ror.org/02ymw8z06","country_code":"US","type":"education","lineage":["https://openalex.org/I76835614"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jorge G. G\u00f3mez-Guti\u00e9rrez","raw_affiliation_strings":["1University of Missouri-Columbia, Columbia, MO;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1University of Missouri-Columbia, Columbia, MO;","institution_ids":["https://openalex.org/I76835614"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5039211276","display_name":"Martin Ramos-Gonzalez","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I76835614","display_name":"University of Missouri","ror":"https://ror.org/02ymw8z06","country_code":"US","type":"education","lineage":["https://openalex.org/I76835614"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Martin Ramos-Gonzalez","raw_affiliation_strings":["1University of Missouri-Columbia, Columbia, MO;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1University of Missouri-Columbia, Columbia, MO;","institution_ids":["https://openalex.org/I76835614"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5089899799","display_name":"Rodolfo Garza-Morales","orcid":"https://orcid.org/0000-0002-3613-521X","observed_orcids":["https://orcid.org/0000-0002-3613-521X"]},"institutions":[{"id":"https://openalex.org/I2802326326","display_name":"The University of Texas Rio Grande Valley","ror":"https://ror.org/02p5xjf12","country_code":"US","type":"education","lineage":["https://openalex.org/I2802326326"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Rodolfo Garza-Morales","raw_affiliation_strings":["2University of Texas Rio Grande Valley, Edinburg, TX;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"2University of Texas Rio Grande Valley, Edinburg, TX;","institution_ids":["https://openalex.org/I2802326326"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5062353028","display_name":"Mohammad Tarique","orcid":"https://orcid.org/0000-0003-4890-8934","observed_orcids":["https://orcid.org/0000-0003-4890-8934"]},"institutions":[{"id":"https://openalex.org/I76835614","display_name":"University of Missouri","ror":"https://ror.org/02ymw8z06","country_code":"US","type":"education","lineage":["https://openalex.org/I76835614"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Mohammad Tarique","raw_affiliation_strings":["1University of Missouri-Columbia, Columbia, MO;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1University of Missouri-Columbia, Columbia, MO;","institution_ids":["https://openalex.org/I76835614"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5085934112","display_name":"Feyza Nur Arguc","orcid":"https://orcid.org/0000-0003-1004-2653","observed_orcids":["https://orcid.org/0000-0003-1004-2653"]},"institutions":[{"id":"https://openalex.org/I76835614","display_name":"University of Missouri","ror":"https://ror.org/02ymw8z06","country_code":"US","type":"education","lineage":["https://openalex.org/I76835614"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Feyza Arguc","raw_affiliation_strings":["1University of Missouri-Columbia, Columbia, MO;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1University of Missouri-Columbia, Columbia, MO;","institution_ids":["https://openalex.org/I76835614"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5091023729","display_name":"Lalit Batra","orcid":"https://orcid.org/0000-0002-4375-9004","observed_orcids":["https://orcid.org/0000-0002-4375-9004"]},"institutions":[{"id":"https://openalex.org/I142740786","display_name":"University of Louisville","ror":"https://ror.org/01ckdn478","country_code":"US","type":"education","lineage":["https://openalex.org/I142740786"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Lalit Batra","raw_affiliation_strings":["3University of Louisville, Louisville, KY"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"3University of Louisville, Louisville, KY","institution_ids":["https://openalex.org/I142740786"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5049976699","display_name":"Haval Shirwan","orcid":"https://orcid.org/0000-0002-1657-9470","observed_orcids":["https://orcid.org/0000-0002-1657-9470"]},"institutions":[{"id":"https://openalex.org/I76835614","display_name":"University of Missouri","ror":"https://ror.org/02ymw8z06","country_code":"US","type":"education","lineage":["https://openalex.org/I76835614"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Haval Shirwan","raw_affiliation_strings":["1University of Missouri-Columbia, Columbia, MO;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1University of Missouri-Columbia, Columbia, MO;","institution_ids":["https://openalex.org/I76835614"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5077060710","display_name":"Esma S. Yolcu","orcid":"https://orcid.org/0000-0002-4312-8798","observed_orcids":["https://orcid.org/0000-0002-4312-8798"]},"institutions":[{"id":"https://openalex.org/I76835614","display_name":"University of Missouri","ror":"https://ror.org/02ymw8z06","country_code":"US","type":"education","lineage":["https://openalex.org/I76835614"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Esma Yolcu","raw_affiliation_strings":["1University of Missouri-Columbia, Columbia, MO;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1University of Missouri-Columbia, Columbia, MO;","institution_ids":["https://openalex.org/I76835614"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"82","issue":"12_Supplement","first_page":"3286","last_page":"3286"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.9911999702453613,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.9911999702453613,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10580","display_name":"Immunotherapy and Immune Responses","score":0.007899999618530273,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10158","display_name":"Cancer Immunotherapy and Biomarkers","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/oncolytic-adenovirus","display_name":"oncolytic adenovirus","score":0.9940000176429749},{"id":"https://openalex.org/keywords/4-1bbl","display_name":"4-1BBL","score":0.9330000281333923},{"id":"https://openalex.org/keywords/streptavidin","display_name":"streptavidin","score":0.7269999980926514},{"id":"https://openalex.org/keywords/immunoadjuvant","display_name":"immunoadjuvant","score":0.7200000286102295},{"id":"https://openalex.org/keywords/damage-associated-molecular-patterns","display_name":"damage-associated molecular patterns","score":0.968999981880188},{"id":"https://openalex.org/keywords/hmgb1","display_name":"HMGB1","score":0.9620000123977661},{"id":"https://openalex.org/keywords/calreticulin","display_name":"calreticulin","score":0.9850000143051147},{"id":"https://openalex.org/keywords/tumor-associated-macrophages","display_name":"tumor-associated macrophages","score":0.5540000200271606},{"id":"https://openalex.org/keywords/cd8-t-cells","display_name":"CD8+ T cells","score":0.6420000195503235},{"id":"https://openalex.org/keywords/tumor-infiltrating-lymphocytes","display_name":"tumor-infiltrating lymphocytes","score":0.5329999923706055},{"id":"https://openalex.org/keywords/adenovirus-vectors","display_name":"adenoviral vector","score":0.492000013589859}],"concepts":[{"id":"https://openalex.org/C82210918","wikidata":"https://www.wikidata.org/wiki/Q1560099","display_name":"Oncolytic virus","level":3,"score":0.545767605304718},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5084825754165649},{"id":"https://openalex.org/C8891405","wikidata":"https://www.wikidata.org/wiki/Q1059","display_name":"Immune system","level":2,"score":0.43622657656669617},{"id":"https://openalex.org/C2780674031","wikidata":"https://www.wikidata.org/wiki/Q2012719","display_name":"Cancer immunotherapy","level":4,"score":0.43160149455070496},{"id":"https://openalex.org/C136449434","wikidata":"https://www.wikidata.org/wiki/Q428253","display_name":"Innate immune system","level":3,"score":0.42597341537475586},{"id":"https://openalex.org/C2777283488","wikidata":"https://www.wikidata.org/wiki/Q7095181","display_name":"Oncolytic adenovirus","level":4,"score":0.42574721574783325},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.36666107177734375},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3544924259185791},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.34480488300323486},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.2976732850074768},{"id":"https://openalex.org/C2777701055","wikidata":"https://www.wikidata.org/wiki/Q1427096","display_name":"Immunotherapy","level":3,"score":0.27863791584968567}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1158/1538-7445.am2022-3286","doi":"https://doi.org/10.1158/1538-7445.am2022-3286","is_oa":false,"landing_page_url":"https://doi.org/10.1158/1538-7445.am2022-3286","pdf_url":null,"source":{"id":"https://openalex.org/S168522863","display_name":"Cancer Research","issn_l":"0008-5472","issn":["0008-5472","1538-7445"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320273","host_organization_name":"American Association for Cancer Research","host_organization_lineage":["https://openalex.org/P4310320273"],"host_organization_lineage_names":["American Association for Cancer Research"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer Research","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.9476000070571899}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4393039233","https://openalex.org/W2126314169","https://openalex.org/W2409057299","https://openalex.org/W4313386736","https://openalex.org/W1849585918","https://openalex.org/W2166024764","https://openalex.org/W2323565136","https://openalex.org/W3131184341","https://openalex.org/W2258332793","https://openalex.org/W2784212326"],"abstract_inverted_index":{"Abstract":[0],"Introduction:":[1],"4-1BBL":[2,32,54,68],"is":[3,8,33,96,107],"a":[4,34,49,119,132,274,341],"co-stimulatory":[5],"molecule":[6],"that":[7,38,106,294,351],"particularly":[9],"important":[10],"for":[11,27,122,429],"the":[12,63,143,161,178,203,225,261,326,354,385,417,426],"expansion":[13],"of":[14,19,53,66,84,94,102,115,125,170,205,214,255,411,425],"T":[15],"cells":[16,83],"and":[17,23,75,87,92,98,111,160,177,192,219,243,250,258,309,331,409],"induction":[18],"robust":[20],"CD8+":[21],"T-effector":[22],"memory":[24],"responses,":[25],"critical":[26],"tumor":[28,328,343],"rejection.":[29],"The":[30,147,168,190,267,322],"natural":[31],"trimeric":[35],"transmembrane":[36],"protein":[37,72,241],"has":[39,79],"no":[40],"activity":[41],"in":[42,76,188,273,307,318,340,373],"soluble":[43,51,77],"form.":[44],"To":[45],"overcome":[46],"this":[47,126],"limitation,":[48],"unique":[50],"form":[52],"was":[55,151,172,182,197,210,271],"developed":[56,131],"by":[57,174,184,199,212,247,265],"linking":[58],"core":[59],"streptavidin":[60],"(SA)":[61],"to":[62,138,142,153],"extracellular":[64],"domain":[65],"murine":[67,308],"(SA-4-1BBL).":[69],"This":[70],"chimeric":[71],"forms":[73],"oligomers,":[74,300],"form,":[78],"pleiotropic":[80],"effects":[81],"on":[82],"innate,":[85],"adaptive,":[86],"regulatory":[88],"immunity.":[89],"However,":[90],"production":[91],"purification":[93],"SA-4-1BBL":[95,116,140,159,179,295,358,421],"cost-":[97],"time-intensive.":[99],"Therefore,":[100],"development":[101,124],"an":[103,412],"expression":[104,169],"platform":[105],"more":[108],"efficient,":[109],"translational,":[110],"augments":[112],"anti-tumor":[113,335,386],"efficacy":[114,270,336],"will":[117],"have":[118],"significant":[120],"impact":[121],"clinical":[123],"immune":[127,355,376,387,418],"adjuvant.":[128],"Thus,":[129],"we":[130,292],"novel":[133],"prototype":[134],"oncolytic":[135,413],"adenoviral":[136],"system":[137,150],"deliver":[139],"(OAdSA-4-1BBL)":[141],"solid":[144],"tumors.":[145],"Methods:":[146],"AdenoQuick":[148],"2.0":[149],"used":[152,370],"construct":[154],"two":[155,286],"OAds,":[156],"one":[157],"expressing":[158,163,288,353],"other":[162,380],"SA":[164,289],"(as":[165],"control":[166,338],"virus).":[167],"transgenes":[171],"confirmed":[173,330],"western":[175],"blot":[176],"biological":[180],"function":[181],"evaluated":[183,198,246,264,272],"splenocyte":[185],"proliferation":[186],"assay":[187],"vitro.":[189],"OAdSA-4-1BBL":[191,268,332],"OAdSA-mediated":[193],"cancer":[194,312],"killing":[195],"effect":[196],"crystal":[200],"violet":[201],"staining,":[202],"activation":[204],"autophagy-mediate":[206],"immunogenic":[207,304],"cell":[208,305,313,320],"death":[209,306],"assessed":[211],"detection":[213],"autophagy":[215],"markers":[216],"(e.g.,":[217],"LC3-I":[218],"II":[220],"as":[221,223,232],"well":[222],"p62),":[224],"damage-associated":[226],"molecular":[227],"patterns":[228],"(DAMPs)":[229],"molecules":[230],"such":[231],"adenosine":[233],"triphosphate":[234],"(ATP),":[235],"high":[236],"mobility":[237],"group":[238],"box":[239],"1":[240],"(HMGB1),":[242],"calreticulin":[244],"were":[245,263,329],"luminescence,":[248],"ELISA,":[249],"flow":[251],"cytometry,":[252],"respectively.":[253],"Expression":[254],"SA,":[256],"SA-4-1BBL,":[257,291],"Hexon":[259],"within":[260,325],"tumors":[262],"immunohistochemistry.":[266],"therapeutic":[269,362],"C57BL/6":[275],"mice":[276],"bearing":[277],"subcutaneous":[278,327],"TC-1":[279,342],"lung":[280,311,365],"tumor.":[281],"Results:":[282],"We":[283],"successfully":[284],"constructed":[285],"OAds":[287],"or":[290,372,379],"demonstrated":[293],"can":[296],"produce":[297],"biologically":[298],"active":[299],"selectively":[301],"induces":[302],"autophagy-mediated":[303],"human":[310],"lines":[314],"with":[315,375,416],"little":[316],"cytotoxicity":[317],"normal":[319],"lines.":[321],"transgene":[323],"expressions":[324],"shows":[333],"greater":[334],"than":[337],"viruses":[339],"mouse":[344],"model.":[345],"Conclusions:":[346],"Overall,":[347],"our":[348],"data":[349],"suggests":[350],"OAd":[352],"checkpoint":[356,377,419],"stimulator":[357,420],"may":[359],"represent":[360],"alternative":[361],"strategy":[363],"against":[364],"cancer,":[366],"which":[367],"could":[368],"be":[369],"alone":[371],"combination":[374],"inhibitors,":[378],"approaches":[381],"aimed":[382],"at":[383],"enhancing":[384],"response.":[388],"Citation":[389],"Format:":[390],"Jorge":[391],"G.":[392],"G\u00f3mez-Guti\u00e9rrez,":[393],"Martin":[394],"Ramos-Gonzalez,":[395],"Rodolfo":[396],"Garza-Morales,":[397],"Mohammad":[398],"Tarique,":[399],"Feyza":[400],"Arguc,":[401],"Lalit":[402],"Batra,":[403],"Haval":[404],"Shirwan,":[405],"Esma":[406],"Yolcu.":[407],"Development":[408],"characterization":[410],"adenovirus":[414],"armed":[415],"[abstract].":[422],"In:":[423],"Proceedings":[424],"American":[427],"Association":[428],"Cancer":[430,441],"Research":[431],"Annual":[432],"Meeting":[433],"2022;":[434],"2022":[435],"Apr":[436],"8-13.":[437],"Philadelphia":[438],"(PA):":[439],"AACR;":[440],"Res":[442],"2022;82(12_Suppl):Abstract":[443],"nr":[444],"3286.":[445]},"counts_by_year":[],"updated_date":"2026-10-12T09:40:04.860316","created_date":"2022-06-16T00:00:00"}