{"id":"https://openalex.org/W4281860705","doi":"https://doi.org/10.3791/63662","title":"Detecting Wolbachia Strain wAlbB in Aedes albopictus Cell Lines","display_name":"Detecting Wolbachia Strain wAlbB in Aedes albopictus Cell Lines","publication_year":2022,"publication_date":"2022-06-01","ids":{"openalex":"https://openalex.org/W4281860705","doi":"https://doi.org/10.3791/63662","pmid":"https://pubmed.ncbi.nlm.nih.gov/35723456"},"language":"en","primary_location":{"id":"doi:10.3791/63662","doi":"https://doi.org/10.3791/63662","is_oa":false,"landing_page_url":"https://doi.org/10.3791/63662","pdf_url":null,"source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5115603233","display_name":"Ling\u2010Ling Chen","orcid":"https://orcid.org/0000-0002-3005-526X","observed_orcids":["https://orcid.org/0000-0002-3005-526X"]},"institutions":[{"id":"https://openalex.org/I149137203","display_name":"Guiyang Medical University","ror":"https://ror.org/035y7a716","country_code":"CN","type":"education","lineage":["https://openalex.org/I149137203"]},{"id":"https://openalex.org/I4405279996","display_name":"Guizhou Health Vocational College","ror":"https://ror.org/029mrnp05","country_code":"CN","type":"education","lineage":["https://openalex.org/I4405279996"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Lingling Chen","raw_affiliation_strings":["Guizhou Medical University; Guizhou Health Vocational College","Guizhou Health Vocational College","Guizhou Medical University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Guizhou Medical University; Guizhou Health Vocational College","institution_ids":["https://openalex.org/I149137203","https://openalex.org/I4405279996"]},{"raw_affiliation_string":"Guizhou Health Vocational College","institution_ids":["https://openalex.org/I4405279996"]},{"raw_affiliation_string":"Guizhou Medical University","institution_ids":["https://openalex.org/I149137203"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5078617176","display_name":"Qiuqiu Xiao","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I149137203","display_name":"Guiyang Medical University","ror":"https://ror.org/035y7a716","country_code":"CN","type":"education","lineage":["https://openalex.org/I149137203"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Qiuqiu Xiao","raw_affiliation_strings":["Guizhou Medical University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Guizhou Medical University","institution_ids":["https://openalex.org/I149137203"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109749528","display_name":"Mengting Shi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I149137203","display_name":"Guiyang Medical University","ror":"https://ror.org/035y7a716","country_code":"CN","type":"education","lineage":["https://openalex.org/I149137203"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Mengting Shi","raw_affiliation_strings":["Guizhou Medical University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Guizhou Medical University","institution_ids":["https://openalex.org/I149137203"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5066679306","display_name":"Jinzhi Cheng","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I149137203","display_name":"Guiyang Medical University","ror":"https://ror.org/035y7a716","country_code":"CN","type":"education","lineage":["https://openalex.org/I149137203"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Jinzhi Cheng","raw_affiliation_strings":["Guizhou Medical University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Guizhou Medical University","institution_ids":["https://openalex.org/I149137203"]}]},{"author_position":"last","author":{"id":null,"display_name":"Jiahong Wu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I149137203","display_name":"Guiyang Medical University","ror":"https://ror.org/035y7a716","country_code":"CN","type":"education","lineage":["https://openalex.org/I149137203"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Jiahong Wu","raw_affiliation_strings":["Guizhou Medical University; jiahongw@gmc.edu.cn","Guizhou Medical University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Guizhou Medical University; jiahongw@gmc.edu.cn","institution_ids":["https://openalex.org/I149137203"]},{"raw_affiliation_string":"Guizhou Medical University","institution_ids":["https://openalex.org/I149137203"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":"184","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12134","display_name":"Insect symbiosis and bacterial influences","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1109","display_name":"Insect Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12134","display_name":"Insect symbiosis and bacterial influences","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1109","display_name":"Insect Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10166","display_name":"Mosquito-borne diseases and control","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11403","display_name":"Invertebrate Immune Response Mechanisms","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/wolbachia","display_name":"Wolbachia","score":0.9559999704360962},{"id":"https://openalex.org/keywords/aedes-albopictus","display_name":"Aedes albopictus","score":1.0},{"id":"https://openalex.org/keywords/mosquito-cell-lines","display_name":"mosquito cell lines","score":0.6320000290870667},{"id":"https://openalex.org/keywords/endosymbionts","display_name":"endosymbionts","score":0.6480000019073486},{"id":"https://openalex.org/keywords/mosquito-virus-interactions","display_name":"mosquito-virus interactions","score":0.6380000114440918},{"id":"https://openalex.org/keywords/cytoplasmic-incompatibility","display_name":"cytoplasmic incompatibility","score":0.9150000214576721},{"id":"https://openalex.org/keywords/immune-priming","display_name":"immune priming","score":0.7860000133514404}],"concepts":[{"id":"https://openalex.org/C2779356413","wikidata":"https://www.wikidata.org/wiki/Q283526","display_name":"Wolbachia","level":3,"score":0.9767858982086182},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8789305686950684},{"id":"https://openalex.org/C2776853612","wikidata":"https://www.wikidata.org/wiki/Q477918","display_name":"Aedes albopictus","level":4,"score":0.8167865872383118},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.7052246928215027},{"id":"https://openalex.org/C147532639","wikidata":"https://www.wikidata.org/wiki/Q5201375","display_name":"Cytoplasmic incompatibility","level":4,"score":0.5447729825973511},{"id":"https://openalex.org/C92087593","wikidata":"https://www.wikidata.org/wiki/Q427389","display_name":"Vector (molecular biology)","level":4,"score":0.5303780436515808},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.48611950874328613},{"id":"https://openalex.org/C2780688631","wikidata":"https://www.wikidata.org/wiki/Q310911","display_name":"Aedes","level":3,"score":0.4372606873512268},{"id":"https://openalex.org/C166703698","wikidata":"https://www.wikidata.org/wiki/Q201993","display_name":"RNA interference","level":4,"score":0.4159778654575348},{"id":"https://openalex.org/C126831891","wikidata":"https://www.wikidata.org/wiki/Q221673","display_name":"Host (biology)","level":2,"score":0.40695980191230774},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.36894553899765015},{"id":"https://openalex.org/C2777775583","wikidata":"https://www.wikidata.org/wiki/Q1148004","display_name":"Aedes aegypti","level":3,"score":0.2344730794429779},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.20144647359848022},{"id":"https://openalex.org/C533803919","wikidata":"https://www.wikidata.org/wiki/Q30953","display_name":"Dengue fever","level":2,"score":0.15768960118293762},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1566455066204071},{"id":"https://openalex.org/C173758957","wikidata":"https://www.wikidata.org/wiki/Q129270","display_name":"Larva","level":2,"score":0.10809972882270813},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.06286612153053284},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D000072138","descriptor_name":"Mosquito Vectors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000330","descriptor_name":"Aedes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012328","descriptor_name":"RNA Viruses","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D020577","descriptor_name":"Wolbachia","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.3791/63662","doi":"https://doi.org/10.3791/63662","is_oa":false,"landing_page_url":"https://doi.org/10.3791/63662","pdf_url":null,"source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},{"id":"doi:10.3791/63662-v","doi":"https://doi.org/10.3791/63662-v","is_oa":false,"landing_page_url":"https://doi.org/10.3791/63662-v","pdf_url":null,"source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},{"id":"pmid:35723456","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/35723456","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of visualized experiments : JoVE","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.6000000238418579,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":30,"referenced_works":["https://openalex.org/W1494397017","https://openalex.org/W1543675143","https://openalex.org/W1546964919","https://openalex.org/W1592352451","https://openalex.org/W1968186266","https://openalex.org/W1971753295","https://openalex.org/W1972541583","https://openalex.org/W2009246694","https://openalex.org/W2012398198","https://openalex.org/W2017054741","https://openalex.org/W2032791847","https://openalex.org/W2034440822","https://openalex.org/W2034492280","https://openalex.org/W2040392172","https://openalex.org/W2047526274","https://openalex.org/W2050339228","https://openalex.org/W2065433574","https://openalex.org/W2069187605","https://openalex.org/W2071406982","https://openalex.org/W2075877120","https://openalex.org/W2092133388","https://openalex.org/W2133250947","https://openalex.org/W2147402521","https://openalex.org/W2172111809","https://openalex.org/W2181243794","https://openalex.org/W2615046465","https://openalex.org/W2951971928","https://openalex.org/W2980458621","https://openalex.org/W3041772551","https://openalex.org/W4388300590"],"related_works":["https://openalex.org/W3111492701","https://openalex.org/W2203088845","https://openalex.org/W2059105022","https://openalex.org/W3196356344","https://openalex.org/W2069187605","https://openalex.org/W3179050308","https://openalex.org/W2161519868","https://openalex.org/W2994949277","https://openalex.org/W2944462650","https://openalex.org/W4414677459"],"abstract_inverted_index":{"As":[0],"a":[1,73,127,148],"maternally":[2],"harbored":[3],"endosymbiont,":[4],"Wolbachia":[5,81,105,161],"infects":[6],"large":[7],"proportions":[8],"of":[9,19,34,43,58,61,80,101,114,137,147,160],"insect":[10,108,165],"populations.":[11],"Studies":[12],"have":[13],"recently":[14],"reported":[15],"the":[16,32,41,55,59,76,84,99,102,111,135,145,158],"successful":[17],"regulation":[18],"RNA":[20],"virus":[21],"transmission":[22],"using":[23],"Wolbachia-transfected":[24,62],"mosquitoes.":[25],"Key":[26],"strategies":[27],"to":[28,64,97,157],"control":[29],"viruses":[30],"include":[31],"manipulation":[33],"host":[35],"reproduction":[36],"via":[37,46],"cytoplasmic":[38],"incompatibility":[39],"and":[40,49,87,106,123],"inhibition":[42],"viral":[44,65],"transcripts":[45],"immune":[47],"priming":[48],"competition":[50],"for":[51,75,134],"host-derived":[52],"resources.":[53],"However,":[54],"underlying":[56],"mechanisms":[57],"responses":[60],"mosquitoes":[63],"infection":[66,82,162],"are":[67],"poorly":[68],"understood.":[69],"This":[70,151],"paper":[71],"presents":[72],"protocol":[74,130],"in":[77,90,163],"vitro":[78],"identification":[79],"at":[83],"nucleic":[85],"acid":[86],"protein":[88],"levels":[89],"Aedes":[91],"albopictus":[92],"(Diptera:":[93],"Culicidae)":[94],"Aa23":[95],"cells":[96,139],"enhance":[98],"understanding":[100],"interactions":[103],"between":[104],"its":[107],"vectors.":[109],"Through":[110],"combined":[112],"use":[113,146],"polymerase":[115],"chain":[116],"reaction":[117],"(PCR),":[118],"quantitative":[119],"PCR,":[120],"western":[121],"blot,":[122],"immunological":[124],"analytical":[125],"methods,":[126],"standard":[128],"morphologic":[129],"has":[131],"been":[132],"described":[133],"detection":[136,159],"Wolbachia-infected":[138],"that":[140],"is":[141],"more":[142],"accurate":[143],"than":[144],"single":[149],"method.":[150],"approach":[152],"may":[153],"also":[154],"be":[155],"applied":[156],"other":[164],"taxa.":[166]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2022-06-13T00:00:00"}