{"id":"https://openalex.org/W4281662352","doi":"https://doi.org/10.1080/10495398.2022.2077742","title":"Development of TaqMan PCR assay for genotyping SNP rs211250281 of the bovine agpat6 gene","display_name":"Development of TaqMan PCR assay for genotyping SNP rs211250281 of the bovine agpat6 gene","publication_year":2022,"publication_date":"2022-05-28","ids":{"openalex":"https://openalex.org/W4281662352","doi":"https://doi.org/10.1080/10495398.2022.2077742","pmid":"https://pubmed.ncbi.nlm.nih.gov/35635030"},"language":"en","primary_location":{"id":"doi:10.1080/10495398.2022.2077742","doi":"https://doi.org/10.1080/10495398.2022.2077742","is_oa":false,"landing_page_url":"https://doi.org/10.1080/10495398.2022.2077742","pdf_url":null,"source":{"id":"https://openalex.org/S122350538","display_name":"Animal Biotechnology","issn_l":"1049-5398","issn":["1049-5398","1532-2378"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","medline","norway-1","oarl","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320547","host_organization_name":"Taylor & Francis","host_organization_lineage":["https://openalex.org/P4310320547","https://openalex.org/P4310320449"],"host_organization_lineage_names":["Taylor & Francis","Informa"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Animal Biotechnology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5058856437","display_name":"Svetlana Nikolaevna Kovalchuk","orcid":"https://orcid.org/0000-0002-5029-0750","observed_orcids":["https://orcid.org/0000-0002-5029-0750"]},"institutions":[{"id":"https://openalex.org/I4210088846","display_name":"Federal Scientific Center for Animal Husbandry named after Academician L.K. Ernst","ror":"https://ror.org/004914a33","country_code":"RU","type":"facility","lineage":["https://openalex.org/I1313323035","https://openalex.org/I4210088846","https://openalex.org/I4210167168"]}],"countries":["RU"],"is_corresponding":true,"raw_author_name":"Svetlana N. Kovalchuk","raw_affiliation_strings":["Department of Molecular Biotechnology, Institute of Innovative Biotechnologies in Animal Husbandry \u2013 the branch of L.K. Ernst Federal Research Center for Animal Husbandry, Moscow, Russia"],"raw_orcid":"https://orcid.org/0000-0002-5029-0750","affiliations":[{"raw_affiliation_string":"Department of Molecular Biotechnology, Institute of Innovative Biotechnologies in Animal Husbandry \u2013 the branch of L.K. Ernst Federal Research Center for Animal Husbandry, Moscow, Russia","institution_ids":["https://openalex.org/I4210088846"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5030393232","display_name":"Anna Leonidovna Arkhipova","orcid":"https://orcid.org/0000-0002-8835-6671","observed_orcids":["https://orcid.org/0000-0002-8835-6671"]},"institutions":[{"id":"https://openalex.org/I4210088846","display_name":"Federal Scientific Center for Animal Husbandry named after Academician L.K. Ernst","ror":"https://ror.org/004914a33","country_code":"RU","type":"facility","lineage":["https://openalex.org/I1313323035","https://openalex.org/I4210088846","https://openalex.org/I4210167168"]}],"countries":["RU"],"is_corresponding":false,"raw_author_name":"Anna L. Arkhipova","raw_affiliation_strings":["Department of Molecular Biotechnology, Institute of Innovative Biotechnologies in Animal Husbandry \u2013 the branch of L.K. Ernst Federal Research Center for Animal Husbandry, Moscow, Russia"],"raw_orcid":"https://orcid.org/0000-0002-8835-6671","affiliations":[{"raw_affiliation_string":"Department of Molecular Biotechnology, Institute of Innovative Biotechnologies in Animal Husbandry \u2013 the branch of L.K. Ernst Federal Research Center for Animal Husbandry, Moscow, Russia","institution_ids":["https://openalex.org/I4210088846"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5058856437"],"corresponding_institution_ids":["https://openalex.org/I4210088846"],"apc_list":null,"apc_paid":null,"fwci":0.7342,"has_fulltext":false,"cited_by_count":10,"citation_normalized_percentile":{"value":0.62538304,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"34","issue":"7","first_page":"3250","last_page":"3255"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10594","display_name":"Genetic and phenotypic traits in livestock","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10594","display_name":"Genetic and phenotypic traits in livestock","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11468","display_name":"Genetic Mapping and Diversity in Plants and Animals","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12929","display_name":"Animal Genetics and Reproduction","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/single-nucleotide-polymorphisms","display_name":"single nucleotide polymorphisms","score":0.949999988079071},{"id":"https://openalex.org/keywords/dairy-cows","display_name":"dairy cattle","score":0.7950000166893005},{"id":"https://openalex.org/keywords/milk-fat-content","display_name":"milk fat content","score":0.8759999871253967},{"id":"https://openalex.org/keywords/fat-to-protein-ratio","display_name":"fat-to-protein ratio","score":0.8610000014305115},{"id":"https://openalex.org/keywords/triacylglycerol-biosynthesis","display_name":"triglyceride synthesis","score":0.8339999914169312},{"id":"https://openalex.org/keywords/allele-specific-probes","display_name":"allele-specific probes","score":0.5519999861717224},{"id":"https://openalex.org/keywords/high-throughput-genotyping","display_name":"high-throughput genotyping","score":0.6940000057220459}],"concepts":[{"id":"https://openalex.org/C143660080","wikidata":"https://www.wikidata.org/wiki/Q7684883","display_name":"TaqMan","level":4,"score":0.8501898050308228},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8312624096870422},{"id":"https://openalex.org/C163691529","wikidata":"https://www.wikidata.org/wiki/Q7391886","display_name":"SNP genotyping","level":5,"score":0.7610918879508972},{"id":"https://openalex.org/C31467283","wikidata":"https://www.wikidata.org/wiki/Q912147","display_name":"Genotyping","level":4,"score":0.7257869839668274},{"id":"https://openalex.org/C139275648","wikidata":"https://www.wikidata.org/wiki/Q17134011","display_name":"SNP","level":5,"score":0.4636901915073395},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.46175432205200195},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4570493996143341},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.43928125500679016},{"id":"https://openalex.org/C153209595","wikidata":"https://www.wikidata.org/wiki/Q501128","display_name":"Single-nucleotide polymorphism","level":4,"score":0.40917491912841797},{"id":"https://openalex.org/C135763542","wikidata":"https://www.wikidata.org/wiki/Q106016","display_name":"Genotype","level":3,"score":0.38712066411972046},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.3741348385810852}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002417","descriptor_name":"Cattle","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D005838","descriptor_name":"Genotype","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008892","descriptor_name":"Milk","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D010641","descriptor_name":"Phenotype","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020641","descriptor_name":"Polymorphism, Single Nucleotide","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D055106","descriptor_name":"Genome-Wide Association Study","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1080/10495398.2022.2077742","doi":"https://doi.org/10.1080/10495398.2022.2077742","is_oa":false,"landing_page_url":"https://doi.org/10.1080/10495398.2022.2077742","pdf_url":null,"source":{"id":"https://openalex.org/S122350538","display_name":"Animal Biotechnology","issn_l":"1049-5398","issn":["1049-5398","1532-2378"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","medline","norway-1","oarl","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320547","host_organization_name":"Taylor & Francis","host_organization_lineage":["https://openalex.org/P4310320547","https://openalex.org/P4310320449"],"host_organization_lineage_names":["Taylor & Francis","Informa"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Animal Biotechnology","raw_type":"journal-article"},{"id":"pmid:35635030","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/35635030","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Animal biotechnology","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Zero hunger","id":"https://metadata.un.org/sdg/2","score":0.9208999872207642}],"study_designs":[],"awards":[{"id":"https://openalex.org/G149473429","display_name":null,"funder_award_id":"121052600344-8","funder_id":"https://openalex.org/F4320327494","funder_display_name":"Ministry of Science and Higher Education of the Russian Federation"}],"funders":[{"id":"https://openalex.org/F4320327494","display_name":"Ministry of Science and Higher Education of the Russian Federation","ror":null}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":46,"referenced_works":["https://openalex.org/W204846666","https://openalex.org/W1025554228","https://openalex.org/W1518768428","https://openalex.org/W1542823905","https://openalex.org/W1909700405","https://openalex.org/W1911678449","https://openalex.org/W1967455686","https://openalex.org/W1975522965","https://openalex.org/W1976015677","https://openalex.org/W1995876004","https://openalex.org/W1997751006","https://openalex.org/W2005690181","https://openalex.org/W2015432991","https://openalex.org/W2028537606","https://openalex.org/W2028801553","https://openalex.org/W2044872702","https://openalex.org/W2046949985","https://openalex.org/W2047737432","https://openalex.org/W2063542361","https://openalex.org/W2070721758","https://openalex.org/W2104146295","https://openalex.org/W2106213860","https://openalex.org/W2110129827","https://openalex.org/W2134422611","https://openalex.org/W2150705960","https://openalex.org/W2170302205","https://openalex.org/W2171182745","https://openalex.org/W2176699305","https://openalex.org/W2327754435","https://openalex.org/W2765200941","https://openalex.org/W2770094547","https://openalex.org/W2892020074","https://openalex.org/W2933060810","https://openalex.org/W2950866894","https://openalex.org/W3002751811","https://openalex.org/W3014148723","https://openalex.org/W3035027294","https://openalex.org/W3091504354","https://openalex.org/W3098538442","https://openalex.org/W3101768345","https://openalex.org/W3128498492","https://openalex.org/W3185926863","https://openalex.org/W3194119456","https://openalex.org/W3206214935","https://openalex.org/W3211709893","https://openalex.org/W4206197006"],"related_works":["https://openalex.org/W4405313900","https://openalex.org/W3006552082","https://openalex.org/W2892020074","https://openalex.org/W2044872702","https://openalex.org/W6966978742","https://openalex.org/W2128115778","https://openalex.org/W4404076575","https://openalex.org/W4417364457","https://openalex.org/W2355144873","https://openalex.org/W2381166912"],"abstract_inverted_index":{"Milk":[0],"fat":[1,25,60,190],"percentage":[2],"is":[3,12,117],"an":[4,34],"important":[5],"production":[6],"trait":[7],"of":[8,14,17,22,37,75,83,96,99,111,122,127,146,155,177],"dairy":[9,66],"cattle":[10,183],"and":[11,62,103,114,129,165,185],"one":[13],"the":[15,23,41,54,73,84,100,112,123,156,175,178],"goals":[16],"breeding":[18],"programs.":[19],"Over":[20],"95%":[21],"milk":[24,59,189],"accounts":[26],"for":[27,79,173],"triacylglycerols.":[28],"AGPAT6":[29],"(1-acylglycerol-3-phosphate":[30],"O-acyltransferase":[31],"6)":[32],"catalyzes":[33],"intermediary":[35],"step":[36],"triglyceride":[38],"synthesis":[39],"in":[40,53,65],"mammary":[42],"cells.":[43],"Genome-wide":[44],"association":[45,187],"studies":[46],"identified":[47],"SNP":[48,81,179],"rs211250281":[49,82,180],"(g27:":[50],"36520069":[51],"G/T)":[52],"agpat6":[55,86,101],"gene":[56],"associated":[57],"with":[58,188],"content":[61],"fat-to-protein":[63],"ratio":[64],"cattle.":[67],"The":[68,133,144],"article":[69],"presents":[70],"data":[71],"on":[72,119],"development":[74],"TaqMan":[76,106,135,158],"PCR":[77,136,159],"assay":[78,137],"genotyping":[80],"bovine":[85],"gene.":[87],"In":[88],"this":[89],"method,":[90],"a":[91,120,170],"primer":[92],"pair,":[93],"initiating":[94],"amplification":[95],"75-bp":[97],"fragments":[98],"gene,":[102],"two":[104],"allele-specific":[105],"probes":[107],"are":[108],"used.":[109],"Identification":[110],"G":[113],"T":[115],"alleles":[116],"based":[118],"comparison":[121],"final":[124],"fluorescence":[125],"intensity":[126],"FAM":[128],"VIC":[130],"dyes,":[131],"respectively.":[132],"developed":[134,157],"was":[138],"validated":[139],"by":[140],"Sanger":[141],"sequencing":[142],"method.":[143],"results":[145],"both":[147],"methods":[148],"fully":[149],"coincided,":[150],"that":[151],"confirmed":[152],"high":[153],"accuracy":[154],"assay.":[160],"This":[161],"reliable,":[162],"simple,":[163],"rapid,":[164],"high-throughput":[166],"method":[167],"could":[168],"be":[169],"suitable":[171],"tool":[172],"studying":[174],"distribution":[176],"among":[181],"different":[182],"breeds":[184],"its":[186],"content.":[191]},"counts_by_year":[{"year":2026,"cited_by_count":4},{"year":2025,"cited_by_count":4},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1}],"updated_date":"2026-11-13T13:42:25.865203","created_date":"2022-06-13T00:00:00"}