{"id":"https://openalex.org/W426393152","doi":null,"title":"Cloning, Heterologous Expression in Yeast, and Biochemical Characterization of Recombinant Putative Glucosyltransferase Clones 9 and 11 from Grapefruit (Citrus paradisi)","display_name":"Cloning, Heterologous Expression in Yeast, and Biochemical Characterization of Recombinant Putative Glucosyltransferase Clones 9 and 11 from Grapefruit (Citrus paradisi)","publication_year":2012,"publication_date":"2012-01-01","ids":{"openalex":"https://openalex.org/W426393152","mag":"426393152"},"language":"en","primary_location":{"id":"pmh:oai:dc.etsu.edu:etd-2400","doi":null,"is_oa":false,"landing_page_url":"https://dc.etsu.edu/etd/1214","pdf_url":null,"source":{"id":"https://openalex.org/S4377196424","display_name":"Digital Commons - East Tennessee State University (East Tennessee State University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I119443389","host_organization_name":"East Tennessee State University","host_organization_lineage":["https://openalex.org/I119443389"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Electronic Theses and Dissertations","raw_type":"text"},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5076004354","display_name":"Anye Wamucho","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Anye Wamucho","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5076004354"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.105,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":{"value":0.39636485,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11237","display_name":"Plant Gene Expression Analysis","score":0.6474999785423279,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11237","display_name":"Plant Gene Expression Analysis","score":0.6474999785423279,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11882","display_name":"Plant biochemistry and biosynthesis","score":0.3181000053882599,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T14150","display_name":"Biochemical and biochemical processes","score":0.00430000014603138,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/glucosyltransferase","display_name":"glucosyltransferase","score":0.9210000038146973},{"id":"https://openalex.org/keywords/grapefruit","display_name":"grapefruit","score":0.9919999837875366},{"id":"https://openalex.org/keywords/komagataella-phaffii","display_name":"Pichia pastoris","score":0.7250000238418579},{"id":"https://openalex.org/keywords/heterologous-expression","display_name":"heterologous expression","score":0.9070000052452087},{"id":"https://openalex.org/keywords/plant-secondary-metabolites","display_name":"plant secondary metabolites","score":0.7379999756813049},{"id":"https://openalex.org/keywords/phenolic-substrates","display_name":"phenolic substrates","score":0.6890000104904175},{"id":"https://openalex.org/keywords/enzyme-activity-screening","display_name":"enzyme activity screening","score":0.46700000762939453},{"id":"https://openalex.org/keywords/gene-cloning","display_name":"gene cloning","score":0.453000009059906},{"id":"https://openalex.org/keywords/catechol","display_name":"catechol","score":0.574999988079071},{"id":"https://openalex.org/keywords/gentisic-acid","display_name":"gentisic acid","score":0.890999972820282},{"id":"https://openalex.org/keywords/vanillin","display_name":"vanillin","score":0.9089999794960022}],"concepts":[{"id":"https://openalex.org/C3018190196","wikidata":"https://www.wikidata.org/wiki/Q41350","display_name":"Citrus paradisi","level":3,"score":0.9172081351280212},{"id":"https://openalex.org/C2777858569","wikidata":"https://www.wikidata.org/wiki/Q5572361","display_name":"Glucosyltransferase","level":3,"score":0.7771670818328857},{"id":"https://openalex.org/C121050878","wikidata":"https://www.wikidata.org/wiki/Q5135020","display_name":"Cloning (programming)","level":2,"score":0.7669116258621216},{"id":"https://openalex.org/C108305142","wikidata":"https://www.wikidata.org/wiki/Q3062286","display_name":"Heterologous expression","level":4,"score":0.6508448719978333},{"id":"https://openalex.org/C2779222958","wikidata":"https://www.wikidata.org/wiki/Q45422","display_name":"Yeast","level":2,"score":0.6306471824645996},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6248282194137573},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.5427317023277283},{"id":"https://openalex.org/C19924922","wikidata":"https://www.wikidata.org/wiki/Q408351","display_name":"Molecular cloning","level":4,"score":0.4797973930835724},{"id":"https://openalex.org/C2780115692","wikidata":"https://www.wikidata.org/wiki/Q5747302","display_name":"Heterologous","level":3,"score":0.4421177804470062},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3989810049533844},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3262181878089905},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.30419307947158813},{"id":"https://openalex.org/C2781353486","wikidata":"https://www.wikidata.org/wiki/Q146030","display_name":"Rutaceae","level":2,"score":0.21959823369979858},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.20146256685256958},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:dc.etsu.edu:etd-2400","doi":null,"is_oa":false,"landing_page_url":"https://dc.etsu.edu/etd/1214","pdf_url":null,"source":{"id":"https://openalex.org/S4377196424","display_name":"Digital Commons - East Tennessee State University (East Tennessee State University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I119443389","host_organization_name":"East Tennessee State University","host_organization_lineage":["https://openalex.org/I119443389"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Electronic Theses and Dissertations","raw_type":"text"},{"id":"mag:426393152","doi":null,"is_oa":false,"landing_page_url":"http://dc.etsu.edu/cgi/viewcontent.cgi?article=2400&context=etd","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":89,"referenced_works":["https://openalex.org/W34271203","https://openalex.org/W83864354","https://openalex.org/W100260962","https://openalex.org/W100729273","https://openalex.org/W111574033","https://openalex.org/W132906125","https://openalex.org/W164218934","https://openalex.org/W168024801","https://openalex.org/W182577994","https://openalex.org/W1510219302","https://openalex.org/W1577373772","https://openalex.org/W1577549297","https://openalex.org/W1602795254","https://openalex.org/W1625282049","https://openalex.org/W1841400157","https://openalex.org/W1964957783","https://openalex.org/W1974987683","https://openalex.org/W1975404789","https://openalex.org/W1985020020","https://openalex.org/W1985692897","https://openalex.org/W1987407807","https://openalex.org/W1987452533","https://openalex.org/W1996133279","https://openalex.org/W2001638522","https://openalex.org/W2006864838","https://openalex.org/W2007601557","https://openalex.org/W2014489366","https://openalex.org/W2017526934","https://openalex.org/W2018800758","https://openalex.org/W2019056784","https://openalex.org/W2025426338","https://openalex.org/W2025724557","https://openalex.org/W2029262575","https://openalex.org/W2030715990","https://openalex.org/W2031313159","https://openalex.org/W2033924510","https://openalex.org/W2038283764","https://openalex.org/W2040541070","https://openalex.org/W2042312786","https://openalex.org/W2047382548","https://openalex.org/W2048309867","https://openalex.org/W2048695976","https://openalex.org/W2049379345","https://openalex.org/W2050894368","https://openalex.org/W2052519950","https://openalex.org/W2052978721","https://openalex.org/W2053174623","https://openalex.org/W2053397996","https://openalex.org/W2053902869","https://openalex.org/W2056368477","https://openalex.org/W2057838303","https://openalex.org/W2065642786","https://openalex.org/W2075636655","https://openalex.org/W2076487460","https://openalex.org/W2077603133","https://openalex.org/W2085022395","https://openalex.org/W2089820895","https://openalex.org/W2093901029","https://openalex.org/W2096819896","https://openalex.org/W2098588686","https://openalex.org/W2104478003","https://openalex.org/W2110837175","https://openalex.org/W2112252323","https://openalex.org/W2112487249","https://openalex.org/W2113800550","https://openalex.org/W2113968207","https://openalex.org/W2120357935","https://openalex.org/W2120558435","https://openalex.org/W2121868928","https://openalex.org/W2124697128","https://openalex.org/W2125647971","https://openalex.org/W2136233218","https://openalex.org/W2140894416","https://openalex.org/W2142498401","https://openalex.org/W2147201798","https://openalex.org/W2151606576","https://openalex.org/W2156449660","https://openalex.org/W2158258935","https://openalex.org/W2159087783","https://openalex.org/W2159514099","https://openalex.org/W2160607514","https://openalex.org/W2165661550","https://openalex.org/W2172155425","https://openalex.org/W2238990854","https://openalex.org/W2313542043","https://openalex.org/W2523557514","https://openalex.org/W2590767013","https://openalex.org/W2796398178","https://openalex.org/W4253134632"],"related_works":["https://openalex.org/W2749054877","https://openalex.org/W206944098","https://openalex.org/W71229738","https://openalex.org/W2748475010","https://openalex.org/W7134723801","https://openalex.org/W2749262432","https://openalex.org/W7005583102","https://openalex.org/W69783964","https://openalex.org/W2273633352","https://openalex.org/W7134643121"],"abstract_inverted_index":{"Flavonoids":[0],"are":[1,76,82],"plant":[2,77],"secondary":[3,32,78],"metabolites":[4],"that":[5,70],"play":[6],"diverse":[7],"roles":[8],"in":[9,16,20],"plants":[10],"and":[11,49,74,81,98,103,121,145],"human":[12],"health.":[13],"These":[14],"compounds":[15],"most":[17],"part":[18],"exist":[19],"the":[21,37,47,96,128],"glucosylated":[22,29],"form.":[23],"Grapefruit":[24],"accumulates":[25],"high":[26],"levels":[27],"of":[28,54,111],"flavonoids.":[30],"Plant":[31],"product":[33,79],"glucosyltransferases":[34],"(GTs)":[35],"catalyze":[36],"glucosylation":[38],"reaction,":[39],"but":[40],"due":[41],"to":[42,61],"low":[43],"homology":[44],"at":[45],"both":[46],"nucleotide":[48],"amino":[50],"acid":[51,123],"sequence":[52,66],"level":[53],"different":[55],"GTs,":[56],"it":[57],"is":[58],"not":[59],"possible":[60],"ascribe":[62],"function":[63],"based":[64],"on":[65],"only.":[67],"The":[68],"hypotheses":[69],"PGT":[71,87,129,132],"clones":[72],"9":[73,88,130],"11":[75,133],"GTs":[80],"biochemically":[83],"regulated":[84],"were":[85],"tested.":[86],"has":[89,134],"been":[90,135],"cloned":[91,137],"into":[92,138,142],"Pichia":[93],"pastoris":[94],"using":[95],"pPICZA":[97,139],"pPICZA\u03b1":[99],"vectors,":[100],"expressed,":[101],"enriched,":[102],"screened":[104],"for":[105,127,140],"GT":[106],"activity":[107],"with":[108],"a":[109],"variety":[110],"phenolic":[112],"substrates.":[113],"Initial":[114],"screens":[115],"show":[116],"catechol,":[117],"gentisic":[118],"acid,":[119],"vanillin,":[120],"p-hydroxylphenylacetic":[122],"as":[124],"potential":[125],"substrates":[126],"protein.":[131],"successfully":[136],"transformation":[141],"yeast,":[143],"expression,":[144],"subsequent":[146],"characterization.":[147]},"counts_by_year":[{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-12T09:40:04.860316","created_date":"2016-06-24T00:00:00"}