{"id":"https://openalex.org/W4255811296","doi":"https://doi.org/10.1097/00000374-199704000-00024","title":"Glycerol Increases Content and Activity of Human Cytochrome P-4502E1 in a Transduced HepG2 Cell Line by Protein Stabilization","display_name":"Glycerol Increases Content and Activity of Human Cytochrome P-4502E1 in a Transduced HepG2 Cell Line by Protein Stabilization","publication_year":1997,"publication_date":"1997-04-01","ids":{"openalex":"https://openalex.org/W4255811296","doi":"https://doi.org/10.1097/00000374-199704000-00024","mag":"2065746750","pmid":"https://pubmed.ncbi.nlm.nih.gov/9113273"},"language":"en","primary_location":{"id":"doi:10.1097/00000374-199704000-00024","doi":"https://doi.org/10.1097/00000374-199704000-00024","is_oa":false,"landing_page_url":"https://doi.org/10.1097/00000374-199704000-00024","pdf_url":null,"source":{"id":"https://openalex.org/S115510430","display_name":"Alcoholism Clinical and Experimental Research","issn_l":"0145-6008","issn":["0145-6008","1530-0277","2993-7175"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-1","medline","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Alcoholism: Clinical & Experimental Research","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Ming-Xue Yang","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Ming-Xue Yang","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5046167919","display_name":"Arthur I. Cederbaum","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Arthur I. Cederbaum","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":4110,"currency":"USD","value_usd":4110},"apc_paid":null,"fwci":0.4775,"has_fulltext":false,"cited_by_count":18,"citation_normalized_percentile":{"value":0.54628225,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"21","issue":"2","first_page":"340","last_page":"340"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10375","display_name":"Pharmacogenetics and Drug Metabolism","score":0.9911999702453613,"subfield":{"id":"https://openalex.org/subfields/3004","display_name":"Pharmacology"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10375","display_name":"Pharmacogenetics and Drug Metabolism","score":0.9911999702453613,"subfield":{"id":"https://openalex.org/subfields/3004","display_name":"Pharmacology"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11465","display_name":"Drug-Induced Hepatotoxicity and Protection","score":0.002300000051036477,"subfield":{"id":"https://openalex.org/subfields/3004","display_name":"Pharmacology"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11747","display_name":"Metal-Catalyzed Oxygenation Mechanisms","score":0.0008999999845400453,"subfield":{"id":"https://openalex.org/subfields/1604","display_name":"Inorganic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/glycerol","display_name":"glycerol","score":0.9940000176429749},{"id":"https://openalex.org/keywords/cyp2e1","display_name":"CYP2E1","score":0.9589999914169312},{"id":"https://openalex.org/keywords/protein-stabilization","display_name":"protein stabilization","score":0.9700000286102295},{"id":"https://openalex.org/keywords/protein-half-life","display_name":"protein half-life","score":0.7730000019073486},{"id":"https://openalex.org/keywords/proteolysis","display_name":"proteolysis","score":0.8420000076293945},{"id":"https://openalex.org/keywords/hepg2-cells","display_name":"HepG2 cells","score":0.8289999961853027},{"id":"https://openalex.org/keywords/ethanol","display_name":"ethanol","score":0.6819999814033508},{"id":"https://openalex.org/keywords/4-methylpyrazole","display_name":"4-methylpyrazole","score":0.9990000128746033},{"id":"https://openalex.org/keywords/cytochrome-b5","display_name":"cytochrome b5","score":0.7979999780654907},{"id":"https://openalex.org/keywords/nadph-cytochrome-p450-reductase","display_name":"NADPH-cytochrome P450 reductase","score":0.9369999766349792},{"id":"https://openalex.org/keywords/nadh-cytochrome-b5-reductase","display_name":"NADH-cytochrome b5 reductase","score":0.9350000023841858},{"id":"https://openalex.org/keywords/microsomes","display_name":"microsomes","score":0.4519999921321869}],"concepts":[{"id":"https://openalex.org/C2780881558","wikidata":"https://www.wikidata.org/wiki/Q132501","display_name":"Glycerol","level":2,"score":0.6832391023635864},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5339155793190002},{"id":"https://openalex.org/C29311851","wikidata":"https://www.wikidata.org/wiki/Q408223","display_name":"Cytochrome c","level":3,"score":0.5231500864028931},{"id":"https://openalex.org/C198352243","wikidata":"https://www.wikidata.org/wiki/Q37105","display_name":"Line (geometry)","level":2,"score":0.481623113155365},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.4790804982185364},{"id":"https://openalex.org/C2780768313","wikidata":"https://www.wikidata.org/wiki/Q408068","display_name":"Cytochrome","level":3,"score":0.4603433907032013},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3804023861885071},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.37170931696891785},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2276807427406311},{"id":"https://openalex.org/C190283241","wikidata":"https://www.wikidata.org/wiki/Q14599311","display_name":"Apoptosis","level":2,"score":0.2143661379814148},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.11348938941955566},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.07852056622505188},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.07001811265945435},{"id":"https://openalex.org/C2524010","wikidata":"https://www.wikidata.org/wiki/Q8087","display_name":"Geometry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002461","descriptor_name":"Cell Line, Transformed","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004795","descriptor_name":"Enzyme Stability","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D005990","descriptor_name":"Glycerol","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008114","descriptor_name":"Liver Neoplasms, Experimental","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010450","descriptor_name":"Endopeptidases","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D014161","descriptor_name":"Transduction, Genetic","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D014161","descriptor_name":"Transduction, Genetic","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D019392","descriptor_name":"Cytochrome P-450 CYP2E1","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1097/00000374-199704000-00024","doi":"https://doi.org/10.1097/00000374-199704000-00024","is_oa":false,"landing_page_url":"https://doi.org/10.1097/00000374-199704000-00024","pdf_url":null,"source":{"id":"https://openalex.org/S115510430","display_name":"Alcoholism Clinical and Experimental Research","issn_l":"0145-6008","issn":["0145-6008","1530-0277","2993-7175"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-1","medline","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Alcoholism: Clinical & Experimental Research","raw_type":"journal-article"},{"id":"pmid:9113273","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9113273","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Alcoholism, clinical and experimental research","raw_type":"Journal Article"},{"id":"mag:2065746750","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9113273","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.8048999905586243,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":36,"referenced_works":["https://openalex.org/W74213787","https://openalex.org/W1493785014","https://openalex.org/W1535053435","https://openalex.org/W1541363280","https://openalex.org/W1562445183","https://openalex.org/W1577117187","https://openalex.org/W1588262878","https://openalex.org/W1775749144","https://openalex.org/W1898017580","https://openalex.org/W1944462698","https://openalex.org/W1966266567","https://openalex.org/W1974655296","https://openalex.org/W1978318306","https://openalex.org/W1978724417","https://openalex.org/W1980609328","https://openalex.org/W1984526747","https://openalex.org/W1991726159","https://openalex.org/W1991812553","https://openalex.org/W2000211178","https://openalex.org/W2041258806","https://openalex.org/W2042664740","https://openalex.org/W2047535702","https://openalex.org/W2055259016","https://openalex.org/W2062453452","https://openalex.org/W2063902118","https://openalex.org/W2067303879","https://openalex.org/W2069816958","https://openalex.org/W2075372903","https://openalex.org/W2078722601","https://openalex.org/W2100837269","https://openalex.org/W2101108802","https://openalex.org/W2116300323","https://openalex.org/W2136839357","https://openalex.org/W2138145189","https://openalex.org/W2159946208","https://openalex.org/W2291865987"],"related_works":["https://openalex.org/W2030105250","https://openalex.org/W2078722601","https://openalex.org/W2072244763","https://openalex.org/W2404173325","https://openalex.org/W1846524227","https://openalex.org/W2970778265","https://openalex.org/W7006040206","https://openalex.org/W2614084968","https://openalex.org/W2782756158","https://openalex.org/W4242330258"],"abstract_inverted_index":{"Glycerol":[0,98,141,176],"is":[1],"widely":[2],"used":[3],"to":[4,12,52,56,65,87,94,123,180,230],"stabilize":[5,231],"cytochrome":[6,13,26,106],"P-450":[7,40,112],"and":[8,22,36,45,71,129,137,150,163,242,247],"prevent":[9],"its":[10],"transformation":[11],"P-420.":[14],"The":[15],"effect":[16,101],"of":[17,24,47,50,73,92,105,110,121,135,147,165,172,185,198,215,239,249],"glycerol":[18,55,93,189,224],"on":[19,102],"the":[20,57,69,79,95,103,119,178,196,237,240,245],"content":[21,70,104,128,149,246],"activity":[23,72,130,248],"human":[25,228],"P-4502E1":[27],"(CYP2E1)":[28],"in":[29,61,68,75,217],"a":[30,62,168,212],"HepG2":[31],"cell":[32],"line":[33],"that":[34,205,223],"stably":[35],"constitutively":[37],"expresses":[38],"this":[39,145,218],"was":[41,190],"evaluated":[42],"by":[43,157,188,200,211],"immunoassays":[44],"oxidation":[46],"p-nitrophenol.":[48],"Addition":[49],"100":[51],"200":[53],"mM":[54],"culture":[58,96],"medium":[59],"resulted":[60],"2":[63],"1/2-":[64],"3-fold":[66],"increase":[67,197],"CYP2E1":[74,127,148,153,166,171,186,199,210,229],"microsomes":[76],"isolated":[77],"from":[78],"cells.":[80],"Increases":[81],"could":[82],"be":[83],"observed":[84],"within":[85],"4":[86,138],"8":[88],"hr":[89],"after":[90],"addition":[91,120],"medium.":[97],"had":[99],"no":[100],"b5":[107,116],"or":[108,114,143,193,202],"activities":[109],"NADPH-cytochrome":[111],"reductase":[113],"NADH-cytochrome":[115],"reductase.":[117],"Upon":[118],"cycloheximide":[122],"stop":[124],"protein":[125,187],"synthesis,":[126],"decreased":[131,144],"with":[132,154,160,195,227],"apparent":[133],"half-lives":[134],"6":[136],"hr,":[139],"respectively.":[140],"prevented":[142],"loss":[146],"activity.":[151],"Labeling":[152],"[35S]methionine,":[155],"followed":[156],"pulse-chase":[158],"experiments":[159],"cold":[161],"methionine":[162],"immunoprecipitation":[164],"indicated":[167],"half-life":[169,179,238],"for":[170],"approximately":[173,181],"3":[174],"hr.":[175,183],"increased":[177],"11":[182],"Stabilization":[184],"not":[191],"additive":[192],"synergistic":[194],"ethanol":[201],"4-methylpyrazole,":[203],"suggesting":[204],"all":[206],"three":[207],"agents":[208],"elevate":[209],"similar":[213],"type":[214],"mechanism":[216],"model.":[219],"These":[220],"results":[221],"indicate":[222],"can":[225],"interact":[226],"it":[232],"against":[233],"proteolytic":[234],"degradation,":[235],"increasing":[236],"enzyme":[241],"thereby":[243],"elevating":[244],"CYP2E1.":[250]},"counts_by_year":[{"year":2019,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":2},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-08T12:12:28.789561","created_date":"2022-05-12T00:00:00"}