{"id":"https://openalex.org/W4252865406","doi":"https://doi.org/10.1002/ange.201600073","title":"Reconstitution of the Cytb5\u2013CytP450 Complex in Nanodiscs for Structural Studies using NMR Spectroscopy","display_name":"Reconstitution of the Cytb5\u2013CytP450 Complex in Nanodiscs for Structural Studies using NMR Spectroscopy","publication_year":2016,"publication_date":"2016-02-29","ids":{"openalex":"https://openalex.org/W4252865406","doi":"https://doi.org/10.1002/ange.201600073"},"language":"en","primary_location":{"id":"doi:10.1002/ange.201600073","doi":"https://doi.org/10.1002/ange.201600073","is_oa":false,"landing_page_url":"https://doi.org/10.1002/ange.201600073","pdf_url":null,"source":{"id":"https://openalex.org/S4210200754","display_name":"Angewandte Chemie","issn_l":"0044-8249","issn":["0044-8249","0932-2140","1521-3757"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","norway-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Angewandte Chemie","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5100437822","display_name":"Meng Zhang","orcid":"https://orcid.org/0000-0003-4497-3253","observed_orcids":["https://orcid.org/0000-0003-4497-3253"]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Meng Zhang","raw_affiliation_strings":["Department of Chemistry, University of Michigan, Ann Arbor, MI, 48109-1055 USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of Michigan, Ann Arbor, MI, 48109-1055 USA","institution_ids":["https://openalex.org/I27837315"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5024013299","display_name":"Rui Huang","orcid":"https://orcid.org/0000-0002-4064-6397","observed_orcids":["https://orcid.org/0000-0002-4064-6397"]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Rui Huang","raw_affiliation_strings":["Department of Chemistry, University of Michigan, Ann Arbor, MI, 48109-1055 USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of Michigan, Ann Arbor, MI, 48109-1055 USA","institution_ids":["https://openalex.org/I27837315"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5012158708","display_name":"Rose Ackermann","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Rose Ackermann","raw_affiliation_strings":["Department of Medicinal Chemistry, The Biointerfaces Institute, University of Michigan, North Campus Research Complex, Ann Arbor, MI, 48109 USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicinal Chemistry, The Biointerfaces Institute, University of Michigan, North Campus Research Complex, Ann Arbor, MI, 48109 USA","institution_ids":["https://openalex.org/I27837315"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5040612727","display_name":"Sang\u2010Choul Im","orcid":"https://orcid.org/0000-0001-9529-3819","observed_orcids":["https://orcid.org/0000-0001-9529-3819"]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]},{"id":"https://openalex.org/I4210114445","display_name":"Michigan Medicine","ror":"https://ror.org/01zcpa714","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210114445"]},{"id":"https://openalex.org/I4210122509","display_name":"Ann Arbor VA Medical Center","ror":"https://ror.org/039n0s143","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1322918889","https://openalex.org/I2799886695","https://openalex.org/I4210102099","https://openalex.org/I4210122509","https://openalex.org/I4210127612"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Sang\u2010Choul Im","raw_affiliation_strings":["Department of Anesthesiology, University of Michigan and VA Medical Center, Ann Arbor, MI, 48105 USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Anesthesiology, University of Michigan and VA Medical Center, Ann Arbor, MI, 48105 USA","institution_ids":["https://openalex.org/I27837315","https://openalex.org/I4210114445","https://openalex.org/I4210122509"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5050932910","display_name":"Lucy A. Waskell","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]},{"id":"https://openalex.org/I4210114445","display_name":"Michigan Medicine","ror":"https://ror.org/01zcpa714","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210114445"]},{"id":"https://openalex.org/I4210122509","display_name":"Ann Arbor VA Medical Center","ror":"https://ror.org/039n0s143","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1322918889","https://openalex.org/I2799886695","https://openalex.org/I4210102099","https://openalex.org/I4210122509","https://openalex.org/I4210127612"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Lucy Waskell","raw_affiliation_strings":["Department of Anesthesiology, University of Michigan and VA Medical Center, Ann Arbor, MI, 48105 USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Anesthesiology, University of Michigan and VA Medical Center, Ann Arbor, MI, 48105 USA","institution_ids":["https://openalex.org/I27837315","https://openalex.org/I4210114445","https://openalex.org/I4210122509"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5000784328","display_name":"Anna A. Schwendeman","orcid":"https://orcid.org/0000-0002-8023-8080","observed_orcids":["https://orcid.org/0000-0002-8023-8080"]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Anna Schwendeman","raw_affiliation_strings":["Department of Medicinal Chemistry, The Biointerfaces Institute, University of Michigan, North Campus Research Complex, Ann Arbor, MI, 48109 USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicinal Chemistry, The Biointerfaces Institute, University of Michigan, North Campus Research Complex, Ann Arbor, MI, 48109 USA","institution_ids":["https://openalex.org/I27837315"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5006414766","display_name":"Ayyalusamy Ramamoorthy","orcid":"https://orcid.org/0000-0003-1964-1900","observed_orcids":["https://orcid.org/0000-0003-1964-1900"]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Ayyalusamy Ramamoorthy","raw_affiliation_strings":["Biophysics and Department of Chemistry, University of Michigan, Ann Arbor, MI, 48109-1055 USA","Department of Chemistry, University of Michigan, Ann Arbor, MI, 48109-1055 USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biophysics and Department of Chemistry, University of Michigan, Ann Arbor, MI, 48109-1055 USA","institution_ids":["https://openalex.org/I27837315"]},{"raw_affiliation_string":"Department of Chemistry, University of Michigan, Ann Arbor, MI, 48109-1055 USA","institution_ids":["https://openalex.org/I27837315"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5006414766"],"corresponding_institution_ids":["https://openalex.org/I27837315"],"apc_list":{"value":5790,"currency":"USD","value_usd":5790},"apc_paid":null,"fwci":2.2509,"has_fulltext":false,"cited_by_count":13,"citation_normalized_percentile":{"value":0.8837932,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":96,"max":99},"biblio":{"volume":"128","issue":"14","first_page":"4573","last_page":"4575"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10407","display_name":"Lipid Membrane Structure and Behavior","score":0.9028000235557556,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10407","display_name":"Lipid Membrane Structure and Behavior","score":0.9028000235557556,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11809","display_name":"Advanced NMR Techniques and Applications","score":0.04879999905824661,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.027300000190734863,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cytochrome-p450","display_name":"cytochrome P450","score":0.9440000057220459},{"id":"https://openalex.org/keywords/cytochrome-b5","display_name":"cytochrome b5","score":1.0},{"id":"https://openalex.org/keywords/protein-protein-interfaces","display_name":"protein-protein interfaces","score":0.8930000066757202},{"id":"https://openalex.org/keywords/redox-partners","display_name":"redox partners","score":0.6230000257492065}],"concepts":[{"id":"https://openalex.org/C2780880673","wikidata":"https://www.wikidata.org/wiki/Q6964029","display_name":"Nanodisc","level":4,"score":0.8855602741241455},{"id":"https://openalex.org/C66974803","wikidata":"https://www.wikidata.org/wiki/Q10359898","display_name":"Nuclear magnetic resonance spectroscopy","level":2,"score":0.6925002336502075},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6914623379707336},{"id":"https://openalex.org/C191120209","wikidata":"https://www.wikidata.org/wiki/Q908902","display_name":"Structural biology","level":2,"score":0.5314273834228516},{"id":"https://openalex.org/C2780768313","wikidata":"https://www.wikidata.org/wiki/Q408068","display_name":"Cytochrome","level":3,"score":0.5274791121482849},{"id":"https://openalex.org/C205365616","wikidata":"https://www.wikidata.org/wiki/Q16863771","display_name":"Membrane biology","level":3,"score":0.49947309494018555},{"id":"https://openalex.org/C2779281246","wikidata":"https://www.wikidata.org/wiki/Q172847","display_name":"Peptide","level":2,"score":0.4769655466079712},{"id":"https://openalex.org/C19549132","wikidata":"https://www.wikidata.org/wiki/Q11141463","display_name":"Two-dimensional nuclear magnetic resonance spectroscopy","level":2,"score":0.4444008767604828},{"id":"https://openalex.org/C144647389","wikidata":"https://www.wikidata.org/wiki/Q423042","display_name":"Membrane protein","level":3,"score":0.4105187952518463},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.38010933995246887},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.3713347911834717},{"id":"https://openalex.org/C8010536","wikidata":"https://www.wikidata.org/wiki/Q160398","display_name":"Crystallography","level":1,"score":0.3234105706214905},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.29799339175224304},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.24398967623710632}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1002/ange.201600073","doi":"https://doi.org/10.1002/ange.201600073","is_oa":false,"landing_page_url":"https://doi.org/10.1002/ange.201600073","pdf_url":null,"source":{"id":"https://openalex.org/S4210200754","display_name":"Angewandte Chemie","issn_l":"0044-8249","issn":["0044-8249","0932-2140","1521-3757"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","norway-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Angewandte Chemie","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":21,"referenced_works":["https://openalex.org/W1134064717","https://openalex.org/W1597150712","https://openalex.org/W1987542967","https://openalex.org/W1993242372","https://openalex.org/W1997765587","https://openalex.org/W2001490774","https://openalex.org/W2003774724","https://openalex.org/W2015091165","https://openalex.org/W2034284608","https://openalex.org/W2035899761","https://openalex.org/W2050731732","https://openalex.org/W2051490548","https://openalex.org/W2067217155","https://openalex.org/W2079619903","https://openalex.org/W2080832034","https://openalex.org/W2093929298","https://openalex.org/W2117213358","https://openalex.org/W2147177386","https://openalex.org/W2164993892","https://openalex.org/W2166025323","https://openalex.org/W2432412291"],"related_works":["https://openalex.org/W2869955696","https://openalex.org/W2977475956","https://openalex.org/W2986437725","https://openalex.org/W3000436964","https://openalex.org/W2745194654","https://openalex.org/W4299821050","https://openalex.org/W4245470654","https://openalex.org/W2076121982","https://openalex.org/W1993242372","https://openalex.org/W80056783"],"abstract_inverted_index":{"Abstract":[0],"Cytochrome":[1],"P450s":[2],"(P450s)":[3],"are":[4,83,96],"a":[5,14,117,121],"superfamily":[6],"of":[7,13,17,42,47,69,103,116],"enzymes":[8],"responsible":[9],"for":[10,38],"the":[11,39,45,48,70,100,104,108,112],"catalysis":[12],"wide":[15],"range":[16],"substrates.":[18],"Dynamic":[19],"interactions":[20],"between":[21],"full\u2010length":[22],"membrane\u2010bound":[23,52,137],"P450":[24],"and":[25,89,127],"its":[26],"redox":[27],"partner":[28],"cytochrome":[29],"b5":[30],"(cytb5)":[31],"have":[32],"been":[33,80],"found":[34],"to":[35,98,131],"be":[36,129],"important":[37],"enzymatic":[40],"activity":[41],"P450.":[43],"However,":[44],"stability":[46],"circa":[49],"70":[50],"kDa":[51],"complex":[53,72,106,119],"in":[54,73,107,120],"model":[55],"membranes":[56],"renders":[57],"high\u2010resolution":[58],"structural":[59,125,134],"NMR":[60,90,94,124],"studies":[61,126],"particularly":[62],"difficult.":[63],"To":[64],"overcome":[65],"these":[66],"challenges,":[67],"reconstitution":[68],"P450\u2013cytb5":[71,105],"peptide\u2010based":[74],"nanodiscs,":[75],"containing":[76],"no":[77],"detergents,":[78],"has":[79],"demonstrated,":[81],"which":[82],"characterized":[84],"by":[85],"size":[86],"exclusion":[87],"chromatography":[88],"spectroscopy.":[91],"In":[92],"addition,":[93],"experiments":[95],"used":[97],"identify":[99],"binding":[101],"interface":[102],"nanodisc.":[109],"This":[110],"is":[111],"first":[113],"successful":[114],"demonstration":[115],"protein\u2013protein":[118],"nanodisc":[122],"using":[123],"should":[128],"useful":[130],"obtain":[132],"valuable":[133],"information":[135],"on":[136],"protein":[138],"complexes.":[139]},"counts_by_year":[{"year":2018,"cited_by_count":6},{"year":2017,"cited_by_count":4},{"year":2016,"cited_by_count":3}],"updated_date":"2026-10-30T12:39:25.321706","created_date":"2022-05-12T00:00:00"}