{"id":"https://openalex.org/W4249954898","doi":"https://doi.org/10.36721/pjps.2020.33.1.reg.135-139.1","title":"Cytotoxic, ?-amylase inhibitory and thrombolytic activities of organic and aqueous extracts of Bacillus clausii KP10","display_name":"Cytotoxic, ?-amylase inhibitory and thrombolytic activities of organic and aqueous extracts of Bacillus clausii KP10","publication_year":2019,"publication_date":"2019-12-23","ids":{"openalex":"https://openalex.org/W4249954898","doi":"https://doi.org/10.36721/pjps.2020.33.1.reg.135-139.1","mag":"3013434235","pmid":"https://pubmed.ncbi.nlm.nih.gov/32122841"},"language":"en","primary_location":{"id":"doi:10.36721/pjps.2020.33.1.reg.135-139.1","doi":"https://doi.org/10.36721/pjps.2020.33.1.reg.135-139.1","is_oa":true,"landing_page_url":"https://doi.org/10.36721/pjps.2020.33.1.reg.135-139.1","pdf_url":null,"source":{"id":"https://openalex.org/S24112332","display_name":"Pakistan Journal of Pharmaceutical Sciences","issn_l":"1011-601X","issn":["1011-601X","3105-9686"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","medline","norway-1","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4310319291","host_organization_name":"University of Karachi","host_organization_lineage":["https://openalex.org/P4310319291"],"host_organization_lineage_names":["University of Karachi"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Pakistan Journal of Pharmaceutical Sciences","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"https://doi.org/10.36721/pjps.2020.33.1.reg.135-139.1","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5080502577","display_name":"Farzana Nighat","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I61623833","display_name":"University of Agriculture Faisalabad","ror":"https://ror.org/054d77k59","country_code":"PK","type":"education","lineage":["https://openalex.org/I61623833"]}],"countries":["PK"],"is_corresponding":false,"raw_author_name":"Farzana Nighat","raw_affiliation_strings":["Bioactive Molecules Research Lab (BMRL), Department of Biochemistry, University of Agriculture, Faisalabad, Pakistan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Bioactive Molecules Research Lab (BMRL), Department of Biochemistry, University of Agriculture, Faisalabad, Pakistan","institution_ids":["https://openalex.org/I61623833"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5023291712","display_name":"Zahid Mushtaq","orcid":"https://orcid.org/0000-0002-0754-8189","observed_orcids":["https://orcid.org/0000-0002-0754-8189"]},"institutions":[{"id":"https://openalex.org/I61623833","display_name":"University of Agriculture Faisalabad","ror":"https://ror.org/054d77k59","country_code":"PK","type":"education","lineage":["https://openalex.org/I61623833"]}],"countries":["PK"],"is_corresponding":false,"raw_author_name":"Zahid Mushtaq","raw_affiliation_strings":["Bioactive Molecules Research Lab (BMRL), Department of Biochemistry, University of Agriculture, Faisalabad, Pakistan/Molecular Biochemistry Lab, Department of Biochemistry, University of Agriculture, Faisalabad, Pakistan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Bioactive Molecules Research Lab (BMRL), Department of Biochemistry, University of Agriculture, Faisalabad, Pakistan/Molecular Biochemistry Lab, Department of Biochemistry, University of Agriculture, Faisalabad, Pakistan","institution_ids":["https://openalex.org/I61623833"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5001804118","display_name":"Munaza Maqsood","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I61623833","display_name":"University of Agriculture Faisalabad","ror":"https://ror.org/054d77k59","country_code":"PK","type":"education","lineage":["https://openalex.org/I61623833"]}],"countries":["PK"],"is_corresponding":false,"raw_author_name":"Munaza Maqsood","raw_affiliation_strings":["Bioactive Molecules Research Lab (BMRL), Department of Biochemistry, University of Agriculture, Faisalabad, Pakistan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Bioactive Molecules Research Lab (BMRL), Department of Biochemistry, University of Agriculture, Faisalabad, Pakistan","institution_ids":["https://openalex.org/I61623833"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5041234587","display_name":"Muhammad Shahid","orcid":"https://orcid.org/0000-0002-4039-8856","observed_orcids":["https://orcid.org/0000-0002-4039-8856"]},"institutions":[{"id":"https://openalex.org/I61623833","display_name":"University of Agriculture Faisalabad","ror":"https://ror.org/054d77k59","country_code":"PK","type":"education","lineage":["https://openalex.org/I61623833"]}],"countries":["PK"],"is_corresponding":false,"raw_author_name":"Muhammad Shahid","raw_affiliation_strings":["Medicinal Biochemistry Research Laboratory, University of Agriculture, Faisalabad, Pakistan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Medicinal Biochemistry Research Laboratory, University of Agriculture, Faisalabad, Pakistan","institution_ids":["https://openalex.org/I61623833"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100682522","display_name":"Muhammad Asif Hanif","orcid":"https://orcid.org/0000-0002-0660-357X","observed_orcids":["https://orcid.org/0000-0002-0660-357X","https://orcid.org/0000-0002-8250-2797"]},"institutions":[{"id":"https://openalex.org/I61623833","display_name":"University of Agriculture Faisalabad","ror":"https://ror.org/054d77k59","country_code":"PK","type":"education","lineage":["https://openalex.org/I61623833"]}],"countries":["PK"],"is_corresponding":false,"raw_author_name":"Muhammad Asif Hanif","raw_affiliation_strings":["Department of Chemistry, University of Agriculture, Faisalabad, Pakistan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of Agriculture, Faisalabad, Pakistan","institution_ids":["https://openalex.org/I61623833"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5037780425","display_name":"Amer Jamil","orcid":"https://orcid.org/0000-0003-4039-4166","observed_orcids":["https://orcid.org/0000-0003-4039-4166"]},"institutions":[{"id":"https://openalex.org/I61623833","display_name":"University of Agriculture Faisalabad","ror":"https://ror.org/054d77k59","country_code":"PK","type":"education","lineage":["https://openalex.org/I61623833"]}],"countries":["PK"],"is_corresponding":false,"raw_author_name":"Amer Jamil","raw_affiliation_strings":["Molecular Biochemistry Lab, Department of Biochemistry, University of Agriculture, Faisalabad, Pakistan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Molecular Biochemistry Lab, Department of Biochemistry, University of Agriculture, Faisalabad, Pakistan","institution_ids":["https://openalex.org/I61623833"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I61623833"],"apc_list":null,"apc_paid":null,"fwci":0.2684,"has_fulltext":false,"cited_by_count":7,"citation_normalized_percentile":{"value":0.49287801,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":96},"biblio":{"volume":"33","issue":"1","first_page":"135","last_page":"139"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.6685000061988831,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.6685000061988831,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10252","display_name":"Microbial Natural Products and Biosynthesis","score":0.17440000176429749,"subfield":{"id":"https://openalex.org/subfields/2402","display_name":"Applied Microbiology and Biotechnology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11140","display_name":"Natural Antidiabetic Agents Studies","score":0.07180000096559525,"subfield":{"id":"https://openalex.org/subfields/2712","display_name":"Endocrinology, Diabetes and Metabolism"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/thrombolytic-activity","display_name":"thrombolytic activity","score":0.8930000066757202},{"id":"https://openalex.org/keywords/alpha-amylase-inhibition","display_name":"\u03b1-amylase inhibition","score":0.9649999737739563},{"id":"https://openalex.org/keywords/cytotoxicity","display_name":"cytotoxicity","score":0.9800000190734863},{"id":"https://openalex.org/keywords/hemolytic-activity","display_name":"hemolytic activity","score":0.925000011920929},{"id":"https://openalex.org/keywords/clot-lysis","display_name":"clot lysis","score":0.7210000157356262},{"id":"https://openalex.org/keywords/streptokinase","display_name":"streptokinase","score":0.7310000061988831},{"id":"https://openalex.org/keywords/myocardial-infarction","display_name":"myocardial infarction","score":0.8569999933242798},{"id":"https://openalex.org/keywords/diabetes-mellitus","display_name":"diabetes mellitus","score":0.8820000290870667},{"id":"https://openalex.org/keywords/ethyl-acetate-fraction","display_name":"ethyl acetate fraction","score":0.7400000095367432},{"id":"https://openalex.org/keywords/methanolic-fraction","display_name":"methanol fraction","score":0.843999981880188},{"id":"https://openalex.org/keywords/water-soluble-fraction","display_name":"water-soluble fraction","score":0.7369999885559082}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.30999428033828735}],"mesh":[{"descriptor_ui":"D000070019","descriptor_name":"Bacillus clausii","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D000516","descriptor_name":"alpha-Amylases","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":true},{"descriptor_ui":"D005343","descriptor_name":"Fibrinolytic Agents","qualifier_ui":"Q000009","qualifier_name":"adverse effects","is_major_topic":false},{"descriptor_ui":"D005343","descriptor_name":"Fibrinolytic Agents","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D005343","descriptor_name":"Fibrinolytic Agents","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D006461","descriptor_name":"Hemolysis","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010936","descriptor_name":"Plant Extracts","qualifier_ui":"Q000009","qualifier_name":"adverse effects","is_major_topic":false},{"descriptor_ui":"D010936","descriptor_name":"Plant Extracts","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D012997","descriptor_name":"Solvents","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D018515","descriptor_name":"Plant Leaves","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.36721/pjps.2020.33.1.reg.135-139.1","doi":"https://doi.org/10.36721/pjps.2020.33.1.reg.135-139.1","is_oa":true,"landing_page_url":"https://doi.org/10.36721/pjps.2020.33.1.reg.135-139.1","pdf_url":null,"source":{"id":"https://openalex.org/S24112332","display_name":"Pakistan Journal of Pharmaceutical Sciences","issn_l":"1011-601X","issn":["1011-601X","3105-9686"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","medline","norway-1","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4310319291","host_organization_name":"University of Karachi","host_organization_lineage":["https://openalex.org/P4310319291"],"host_organization_lineage_names":["University of Karachi"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Pakistan Journal of Pharmaceutical Sciences","raw_type":"journal-article"},{"id":"pmid:32122841","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/32122841","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Pakistan journal of pharmaceutical sciences","raw_type":"Journal Article"},{"id":"mag:3013434235","doi":null,"is_oa":true,"landing_page_url":"https://europepmc.org/article/MED/32122841","pdf_url":null,"source":{"id":"https://openalex.org/S24112332","display_name":"Pakistan Journal of Pharmaceutical Sciences","issn_l":"1011-601X","issn":["1011-601X","3105-9686"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","medline","norway-1","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4310319291","host_organization_name":"University of Karachi","host_organization_lineage":["https://openalex.org/P4310319291"],"host_organization_lineage_names":["University of Karachi"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Pakistan Journal of Pharmaceutical Sciences","raw_type":null}],"best_oa_location":{"id":"doi:10.36721/pjps.2020.33.1.reg.135-139.1","doi":"https://doi.org/10.36721/pjps.2020.33.1.reg.135-139.1","is_oa":true,"landing_page_url":"https://doi.org/10.36721/pjps.2020.33.1.reg.135-139.1","pdf_url":null,"source":{"id":"https://openalex.org/S24112332","display_name":"Pakistan Journal of Pharmaceutical Sciences","issn_l":"1011-601X","issn":["1011-601X","3105-9686"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","medline","norway-1","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4310319291","host_organization_name":"University of Karachi","host_organization_lineage":["https://openalex.org/P4310319291"],"host_organization_lineage_names":["University of Karachi"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Pakistan Journal of Pharmaceutical Sciences","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.9476000070571899,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W3013434235","https://openalex.org/W1989684625","https://openalex.org/W4414623024","https://openalex.org/W1739668385","https://openalex.org/W7097124966","https://openalex.org/W6920268830","https://openalex.org/W3032910281","https://openalex.org/W2047598813","https://openalex.org/W2343352439","https://openalex.org/W4308865200"],"abstract_inverted_index":{"Humans":[0],"are":[1],"experiencing":[2],"serious":[3],"health":[4],"issues":[5],"like":[6],"myocardial":[7,16],"infarction":[8,17],"and":[9,70,80,103],"diabetes.":[10],"Thrombosis":[11],"is":[12,35],"the":[13,31,42,66,78,115,160],"reason":[14],"of":[15,38,44,77,84,183,199],"that":[18],"may":[19],"cause":[20],"death.":[21],"Bioactive":[22],"compounds":[23],"or":[24],"enzymes":[25],"can":[26,51],"be":[27,52],"used":[28],"to":[29,64,156],"dissolve":[30],"clot.":[32],"Whereas":[33],"diabetes":[34],"a":[36],"disorder":[37],"metabolism":[39],"in":[40,46],"which":[41,133,167],"level":[43],"glucose":[45],"blood":[47],"becomes":[48],"high.":[49],"It":[50],"controlled":[53],"by":[54,100,121],"inhibiting":[55],"\u03b1-amylase":[56,68,95,190],"enzyme.":[57],"The":[58,88,146],"current":[59],"project":[60],"was,":[61],"therefore":[62],"planned":[63],"investigate":[65],"thrombolytic,":[67],"inhibitory":[69,191],"cytotoxic":[71,144],"(to":[72],"access":[73],"drug":[74],"safety)":[75],"potentials":[76],"organic":[79],"aqueous":[81],"bioactive":[82],"fractions":[83,141],"Bacillus":[85],"clausii":[86,185],"KP10.":[87],"cytotoxicity":[89],"was":[90,98,107,119],"assessed":[91],"with":[92,109,129],"hemolytic":[93,117,136],"assay,":[94],"inhibition":[96],"assay":[97],"done":[99],"using":[101],"DNS":[102],"in-vitro":[104],"thrombolytic":[105,152],"effect":[106],"checked":[108],"human":[110],"blood.":[111],"In":[112,177],"our":[113,178],"experiments,":[114],"maximum":[116,172,188],"activity":[118,137],"shown":[120],"ethyl":[122],"acetate":[123],"fraction":[124,170,182],"(12.64%).":[125],"Results":[126,193],"were":[127,148,194],"compared":[128],"standard":[130],"Triton":[131],"X-100":[132],"showed":[134,142,162,187],"91.61%":[135],"whereas":[138],"all":[139],"other":[140],"least":[143],"activity.":[145,176,192],"extracts":[147,161],"also":[149],"evaluated":[150],"as":[151,154],"agents":[153],"correlated":[155],"streptokinase":[157],"(73.83%).":[158],"All":[159],"clot":[163,174],"lysis":[164,175],"activity,":[165],"among":[166],"water":[168],"soluble":[169,181],"exhibited":[171],"(35.16%)":[173],"experiment":[179],"methanol":[180],"B.":[184],"KP10":[186],"26.49%":[189],"analyzed":[195],"statistically":[196],"through":[197],"analysis":[198],"variance":[200],"(ANOVA).":[201]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2022-05-12T00:00:00"}