{"id":"https://openalex.org/W4249091591","doi":"https://doi.org/10.1007/978-1-59745-198-7_8","title":"Kinetic Silver Staining of Proteins","display_name":"Kinetic Silver Staining of Proteins","publication_year":2009,"publication_date":"2009-01-01","ids":{"openalex":"https://openalex.org/W4249091591","doi":"https://doi.org/10.1007/978-1-59745-198-7_8"},"language":"en","primary_location":{"id":"doi:10.1007/978-1-59745-198-7_8","doi":"https://doi.org/10.1007/978-1-59745-198-7_8","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-59745-198-7_8","pdf_url":null,"source":{"id":"https://openalex.org/S4210224522","display_name":"Springer protocols handbooks/Springer protocols","issn_l":"1949-2448","issn":["1949-2448","1949-2456"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310315801","host_organization_name":"Humana Press","host_organization_lineage":["https://openalex.org/P4310315801","https://openalex.org/P4310319900"],"host_organization_lineage_names":["Humana Press","Springer Science+Business Media"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Springer Protocols Handbooks","raw_type":"book-chapter"},"volume":{"id":"https://openalex.org/W1500937540","display_name":"The Protein Protocols Handbook"},"type":"book-chapter","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5087156966","display_name":"Douglas D. Root","orcid":"https://orcid.org/0000-0002-6738-6003","observed_orcids":["https://orcid.org/0000-0002-6738-6003"]},"institutions":[{"id":"https://openalex.org/I123534392","display_name":"University of North Texas","ror":"https://ror.org/00v97ad02","country_code":"US","type":"education","lineage":["https://openalex.org/I123534392"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Douglas D. Root","raw_affiliation_strings":["Department of Biological Sciences, University of North Texas, Denton, TX, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Sciences, University of North Texas, Denton, TX, USA","institution_ids":["https://openalex.org/I123534392"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5100707347","display_name":"Kuan Wang","orcid":"https://orcid.org/0000-0002-8282-2002","observed_orcids":["https://orcid.org/0000-0002-8282-2002"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210087962","display_name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","ror":"https://ror.org/006zn3t30","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210087962"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kuan Wang","raw_affiliation_strings":["Laboratory of Muscle Biology, NIH, NIAMS/LPB, Bethesda, MD, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Muscle Biology, NIH, NIAMS/LPB, Bethesda, MD, USA","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210087962"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.66605098,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":"51","last_page":"54"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.8363999724388123,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.8363999724388123,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12867","display_name":"Advanced Biosensing Techniques and Applications","score":0.12020000070333481,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.0066999997943639755,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/protein-quantification","display_name":"protein quantification","score":0.9380000233650208},{"id":"https://openalex.org/keywords/microtiter-plate-assay","display_name":"microtiter plate assay","score":0.7269999980926514},{"id":"https://openalex.org/keywords/protein-adsorption","display_name":"protein adsorption","score":0.7160000205039978},{"id":"https://openalex.org/keywords/thin-layer-chromatography","display_name":"thin-layer chromatography","score":0.7459999918937683},{"id":"https://openalex.org/keywords/cellulose","display_name":"cellulose","score":0.3970000147819519},{"id":"https://openalex.org/keywords/elisa","display_name":"ELISA","score":0.4519999921321869}],"concepts":[{"id":"https://openalex.org/C74864618","wikidata":"https://www.wikidata.org/wiki/Q2332446","display_name":"Staining","level":2,"score":0.8427616357803345},{"id":"https://openalex.org/C2778810118","wikidata":"https://www.wikidata.org/wiki/Q534260","display_name":"Microtiter plate","level":2,"score":0.7502226829528809},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6764994859695435},{"id":"https://openalex.org/C25330733","wikidata":"https://www.wikidata.org/wiki/Q907841","display_name":"Silver stain","level":2,"score":0.6251952052116394},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.6210027933120728},{"id":"https://openalex.org/C2778476535","wikidata":"https://www.wikidata.org/wiki/Q390697","display_name":"Agarose","level":2,"score":0.47826850414276123},{"id":"https://openalex.org/C86807702","wikidata":"https://www.wikidata.org/wiki/Q2620381","display_name":"Bovine serum albumin","level":2,"score":0.4499184489250183},{"id":"https://openalex.org/C2778862431","wikidata":"https://www.wikidata.org/wiki/Q416002","display_name":"Polyacrylamide","level":2,"score":0.4199838638305664},{"id":"https://openalex.org/C101660505","wikidata":"https://www.wikidata.org/wiki/Q898301","display_name":"Polyacrylamide gel electrophoresis","level":3,"score":0.4118501543998718},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3245583474636078},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3195444643497467},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.19934192299842834},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.14211943745613098},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0},{"id":"https://openalex.org/C188027245","wikidata":"https://www.wikidata.org/wiki/Q750446","display_name":"Polymer chemistry","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1007/978-1-59745-198-7_8","doi":"https://doi.org/10.1007/978-1-59745-198-7_8","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-59745-198-7_8","pdf_url":null,"source":{"id":"https://openalex.org/S4210224522","display_name":"Springer protocols handbooks/Springer protocols","issn_l":"1949-2448","issn":["1949-2448","1949-2456"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310315801","host_organization_name":"Humana Press","host_organization_lineage":["https://openalex.org/P4310315801","https://openalex.org/P4310319900"],"host_organization_lineage_names":["Humana Press","Springer Science+Business Media"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Springer Protocols Handbooks","raw_type":"book-chapter"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":4,"referenced_works":["https://openalex.org/W1984956543","https://openalex.org/W2001980216","https://openalex.org/W2011925207","https://openalex.org/W2056886528"],"related_works":["https://openalex.org/W614154043","https://openalex.org/W2056886528","https://openalex.org/W2482977172","https://openalex.org/W2197372788","https://openalex.org/W2011925207","https://openalex.org/W2071577990","https://openalex.org/W2358460980","https://openalex.org/W2037034632","https://openalex.org/W2011730787","https://openalex.org/W2014081535"],"abstract_inverted_index":{"Silver":[0,26],"staining":[1,27,80,108,125,168],"methods":[2],"have":[3],"long":[4],"been":[5,94],"know":[6],"to":[7,32,49,105,126,157,172],"provide":[8],"highly":[9],"sensitive":[10,162,175],"detection":[11,178],"of":[12,46,86,154,179],"proteins":[13,47,180],"and":[14,21,41,61,207,216],"nucleic":[15],"acids":[16],"following":[17],"electrophoresis":[18],"in":[19,73,89],"agarose":[20],"polyacrylamide":[22],"gels":[23],"(1,":[24],"2).":[25],"technologies":[28],"can":[29,144],"be":[30,145,173,220],"extended":[31],"other":[33,198],"media":[34],"such":[35,183],"as":[36,148,184,222],"blots,":[37],"thin-layer":[38],"chromatography":[39],"(TLC),":[40],"microtiter":[42,50,91,98],"plates.":[43],"The":[44,77,97],"quantification":[45,117],"adsorbed":[48,87],"plate":[51,92,99],"wells":[52],"provides":[53],"quantitative":[54],"information":[55],"for":[56,82,124,142,176,188,194],"enzyme-linked":[57],"immunosorbent":[58],"assay":[59,100,160,169],"(ELISA)":[60],"protein":[62,88,141],"interaction":[63],"assays.":[64],"One":[65],"nonradioactive":[66],"procedure,":[67],"copper":[68,106],"iodide":[69,107],"staining,":[70],"is":[71,118,136,152,161,170,185,214],"described":[72],"Chapter":[74,192],"72":[75,193],"(3).":[76],"kinetic":[78,166],"silver":[79,167],"method":[81],"measuring":[83],"the":[84,121,159,177,210,217,223],"amount":[85],"a":[90,102,128,139,195],"has":[93,101],"developed":[95],"recently.":[96],"sensitivity":[103],"similar":[104],"(5\u2013150":[109],"ng/well)":[110],"but":[111],"higher":[112],"precision":[113],"(<5%;":[114],"4).":[115],"When":[116],"based":[119],"on":[120,181],"time":[122],"required":[123],"reach":[127],"fixed":[129],"optical":[130],"density,":[131],"very":[132],"little":[133],"protein-to-protein":[134],"variation":[135],"observed,":[137],"so":[138],"standard":[140],"calibration":[143],"selected":[146],"(such":[147],"bovine":[149],"albumin)":[150],"that":[151],"free":[153],"interfering":[155],"substances":[156],"which":[158],"(4).":[163],"Furthermore,":[164],"this":[165],"found":[171],"most":[174],"cellulose":[182],"commonly":[186],"used":[187],"TLC":[189],"(":[190],"see":[191],"comparison":[196],"with":[197],"solidphase":[199],"stains).":[200],"These":[201],"keywords":[202,218],"were":[203],"added":[204],"by":[205,209],"machine":[206],"not":[208],"authors.":[211],"This":[212],"process":[213],"experimental":[215],"may":[219],"updated":[221],"learning":[224],"algorithm":[225],"improves.":[226]},"counts_by_year":[],"updated_date":"2026-10-10T09:11:00.788812","created_date":"2022-05-12T00:00:00"}