{"id":"https://openalex.org/W4248915536","doi":"https://doi.org/10.15210/sah.v7i1.14942","title":"DIFERENTES M\u00c9TODOS PARA PROCESSAMENTO DE AMOSTRAS DE C\u00c9LULAS DA GRANULOSA OBTIDAS POR ASPIRA\u00c7\u00c3O FOLICULAR PARA A EXTRA\u00c7\u00c3O DE RNA","display_name":"DIFERENTES M\u00c9TODOS PARA PROCESSAMENTO DE AMOSTRAS DE C\u00c9LULAS DA GRANULOSA OBTIDAS POR ASPIRA\u00c7\u00c3O FOLICULAR PARA A EXTRA\u00c7\u00c3O DE RNA","publication_year":2019,"publication_date":"2019-12-11","ids":{"openalex":"https://openalex.org/W4248915536","doi":"https://doi.org/10.15210/sah.v7i1.14942","mag":"3043929764"},"language":"pt","primary_location":{"id":"doi:10.15210/sah.v7i1.14942","doi":"https://doi.org/10.15210/sah.v7i1.14942","is_oa":true,"landing_page_url":"https://doi.org/10.15210/sah.v7i1.14942","pdf_url":"https://periodicos.ufpel.edu.br/ojs2/index.php/veterinaria/article/download/14942/10733","source":{"id":"https://openalex.org/S4210229503","display_name":"SCIENCE AND ANIMAL HEALTH","issn_l":"2318-356X","issn":["2318-356X"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310312515","host_organization_name":"Federal University of Pelotas","host_organization_lineage":["https://openalex.org/P4310312515"],"host_organization_lineage_names":["Federal University of Pelotas"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Science And Animal Health","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"https://periodicos.ufpel.edu.br/ojs2/index.php/veterinaria/article/download/14942/10733","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5011802544","display_name":"Cristina Sangoi Haas","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I169248161","display_name":"Universidade Federal de Pelotas","ror":"https://ror.org/05msy9z54","country_code":"BR","type":"education","lineage":["https://openalex.org/I169248161"]}],"countries":["BR"],"is_corresponding":false,"raw_author_name":"Cristina Sangoi Haas","raw_affiliation_strings":["Programa de P\u00f3s-Gradua\u00e7\u00e3o em Veterin\u00e1ria, Faculdade de Veterin\u00e1ria, Universidade Federal de Pelotas"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Veterin\u00e1ria, Faculdade de Veterin\u00e1ria, Universidade Federal de Pelotas","institution_ids":["https://openalex.org/I169248161"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5082836346","display_name":"Juliana Germano Ferst","orcid":"https://orcid.org/0000-0002-9888-2245","observed_orcids":["https://orcid.org/0000-0002-9888-2245"]},"institutions":[{"id":"https://openalex.org/I33501960","display_name":"Universidade Federal de Santa Maria","ror":"https://ror.org/01b78mz79","country_code":"BR","type":"education","lineage":["https://openalex.org/I33501960"]}],"countries":["BR"],"is_corresponding":false,"raw_author_name":"Juliana Germano Ferst","raw_affiliation_strings":["Laborat\u00f3rio de Reprodu\u00e7\u00e3o Animal, Universidade Federal de Santa Maria"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laborat\u00f3rio de Reprodu\u00e7\u00e3o Animal, Universidade Federal de Santa Maria","institution_ids":["https://openalex.org/I33501960"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5067650297","display_name":"Camila Amaral D\u2019\u00c1vila","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I169248161","display_name":"Universidade Federal de Pelotas","ror":"https://ror.org/05msy9z54","country_code":"BR","type":"education","lineage":["https://openalex.org/I169248161"]}],"countries":["BR"],"is_corresponding":false,"raw_author_name":"Camila Amaral D\u2019Avila","raw_affiliation_strings":["Programa de P\u00f3s-Gradua\u00e7\u00e3o em Veterin\u00e1ria, Faculdade de Veterin\u00e1ria, Universidade Federal de Pelotas"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Veterin\u00e1ria, Faculdade de Veterin\u00e1ria, Universidade Federal de Pelotas","institution_ids":["https://openalex.org/I169248161"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5061589884","display_name":"Fabiane Pereira de Moraes","orcid":"https://orcid.org/0000-0003-2502-4108","observed_orcids":["https://orcid.org/0000-0003-2502-4108"]},"institutions":[{"id":"https://openalex.org/I169248161","display_name":"Universidade Federal de Pelotas","ror":"https://ror.org/05msy9z54","country_code":"BR","type":"education","lineage":["https://openalex.org/I169248161"]}],"countries":["BR"],"is_corresponding":false,"raw_author_name":"Fabiane Pereira Moraes","raw_affiliation_strings":["Programa de P\u00f3s-Gradua\u00e7\u00e3o em Veterin\u00e1ria, Faculdade de Veterin\u00e1ria, Universidade Federal de Pelotas"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Veterin\u00e1ria, Faculdade de Veterin\u00e1ria, Universidade Federal de Pelotas","institution_ids":["https://openalex.org/I169248161"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5039038932","display_name":"Fernando Caetano de Oliveira","orcid":"https://orcid.org/0000-0002-5191-9901","observed_orcids":["https://orcid.org/0000-0002-5191-9901"]},"institutions":[{"id":"https://openalex.org/I169248161","display_name":"Universidade Federal de Pelotas","ror":"https://ror.org/05msy9z54","country_code":"BR","type":"education","lineage":["https://openalex.org/I169248161"]}],"countries":["BR"],"is_corresponding":false,"raw_author_name":"Fernando Caetano Oliveira","raw_affiliation_strings":["Programa de P\u00f3s-Gradua\u00e7\u00e3o em Veterin\u00e1ria, Faculdade de Veterin\u00e1ria, Universidade Federal de Pelotas"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Veterin\u00e1ria, Faculdade de Veterin\u00e1ria, Universidade Federal de Pelotas","institution_ids":["https://openalex.org/I169248161"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5026520144","display_name":"S\u00e9rgio Farias Vargas J\u00fanior","orcid":"https://orcid.org/0000-0002-2782-4574","observed_orcids":["https://orcid.org/0000-0002-2782-4574"]},"institutions":[{"id":"https://openalex.org/I169248161","display_name":"Universidade Federal de Pelotas","ror":"https://ror.org/05msy9z54","country_code":"BR","type":"education","lineage":["https://openalex.org/I169248161"]}],"countries":["BR"],"is_corresponding":false,"raw_author_name":"Sergio Farias Vargas Jr","raw_affiliation_strings":["Programa de P\u00f3s-Gradua\u00e7\u00e3o em Veterin\u00e1ria, Faculdade de Veterin\u00e1ria, Universidade Federal de Pelotas"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Veterin\u00e1ria, Faculdade de Veterin\u00e1ria, Universidade Federal de Pelotas","institution_ids":["https://openalex.org/I169248161"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5019661974","display_name":"Paulo Bayard Dias Gon\u00e7alves","orcid":"https://orcid.org/0000-0002-0671-1466","observed_orcids":["https://orcid.org/0000-0002-0671-1466"]},"institutions":[{"id":"https://openalex.org/I33501960","display_name":"Universidade Federal de Santa Maria","ror":"https://ror.org/01b78mz79","country_code":"BR","type":"education","lineage":["https://openalex.org/I33501960"]}],"countries":["BR"],"is_corresponding":false,"raw_author_name":"Paulo Bayard Dias Gon\u00e7alves","raw_affiliation_strings":["Laborat\u00f3rio de Reprodu\u00e7\u00e3o Animal, Universidade Federal de Santa Maria"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laborat\u00f3rio de Reprodu\u00e7\u00e3o Animal, Universidade Federal de Santa Maria","institution_ids":["https://openalex.org/I33501960"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5015404652","display_name":"Bernardo Garziera Gasperin","orcid":"https://orcid.org/0000-0002-3715-2345","observed_orcids":["https://orcid.org/0000-0002-3715-2345"]},"institutions":[{"id":"https://openalex.org/I169248161","display_name":"Universidade Federal de Pelotas","ror":"https://ror.org/05msy9z54","country_code":"BR","type":"education","lineage":["https://openalex.org/I169248161"]}],"countries":["BR"],"is_corresponding":false,"raw_author_name":"Bernardo Garziera Gasperin","raw_affiliation_strings":["Universidade Federal de Pelotas"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Universidade Federal de Pelotas","institution_ids":["https://openalex.org/I169248161"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"7","issue":"1","first_page":"68","last_page":"68"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9369999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9369999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10584","display_name":"Reproductive Physiology in Livestock","score":0.031099999323487282,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10364","display_name":"Reproductive Biology and Fertility","score":0.02410000003874302,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/follicular-cells","display_name":"follicular cells","score":0.7039999961853027},{"id":"https://openalex.org/keywords/granulosa-cells","display_name":"granulosa cells","score":0.9070000052452087},{"id":"https://openalex.org/keywords/follicular-aspiration","display_name":"follicular aspiration","score":0.9490000009536743},{"id":"https://openalex.org/keywords/rna-extraction","display_name":"RNA extraction","score":0.8610000014305115},{"id":"https://openalex.org/keywords/sample-processing","display_name":"sample processing","score":0.5989999771118164},{"id":"https://openalex.org/keywords/trizol","display_name":"TRIzol","score":0.796999990940094},{"id":"https://openalex.org/keywords/cell-storage","display_name":"cell storage","score":0.4090000092983246},{"id":"https://openalex.org/keywords/gene-expression","display_name":"gene expression","score":0.6970000267028809},{"id":"https://openalex.org/keywords/ovary","display_name":"ovary","score":0.45100000500679016},{"id":"https://openalex.org/keywords/reference-genes","display_name":"reference genes","score":0.35499998927116394}],"concepts":[{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.581887423992157},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.5271983742713928},{"id":"https://openalex.org/C16685009","wikidata":"https://www.wikidata.org/wiki/Q335057","display_name":"Andrology","level":1,"score":0.38130104541778564},{"id":"https://openalex.org/C29456083","wikidata":"https://www.wikidata.org/wiki/Q1221899","display_name":"Gynecology","level":1,"score":0.3686274290084839},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.29690805077552795},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.19844359159469604}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.15210/sah.v7i1.14942","doi":"https://doi.org/10.15210/sah.v7i1.14942","is_oa":true,"landing_page_url":"https://doi.org/10.15210/sah.v7i1.14942","pdf_url":"https://periodicos.ufpel.edu.br/ojs2/index.php/veterinaria/article/download/14942/10733","source":{"id":"https://openalex.org/S4210229503","display_name":"SCIENCE AND ANIMAL HEALTH","issn_l":"2318-356X","issn":["2318-356X"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310312515","host_organization_name":"Federal University of Pelotas","host_organization_lineage":["https://openalex.org/P4310312515"],"host_organization_lineage_names":["Federal University of Pelotas"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Science And Animal Health","raw_type":"journal-article"},{"id":"mag:3043929764","doi":null,"is_oa":false,"landing_page_url":"https://www.cabdirect.org/cabdirect/abstract/20203163884","pdf_url":null,"source":{"id":"https://openalex.org/S2764678757","display_name":"Science of animal health","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Science of animal health","raw_type":null}],"best_oa_location":{"id":"doi:10.15210/sah.v7i1.14942","doi":"https://doi.org/10.15210/sah.v7i1.14942","is_oa":true,"landing_page_url":"https://doi.org/10.15210/sah.v7i1.14942","pdf_url":"https://periodicos.ufpel.edu.br/ojs2/index.php/veterinaria/article/download/14942/10733","source":{"id":"https://openalex.org/S4210229503","display_name":"SCIENCE AND ANIMAL HEALTH","issn_l":"2318-356X","issn":["2318-356X"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310312515","host_organization_name":"Federal University of Pelotas","host_organization_lineage":["https://openalex.org/P4310312515"],"host_organization_lineage_names":["Federal University of Pelotas"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Science And Animal Health","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.428600013256073,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4248915536.pdf","grobid_xml":"https://content.openalex.org/works/W4248915536.grobid-xml"},"referenced_works_count":10,"referenced_works":["https://openalex.org/W128587512","https://openalex.org/W1984302646","https://openalex.org/W1996549310","https://openalex.org/W2020192585","https://openalex.org/W2093131844","https://openalex.org/W2116341609","https://openalex.org/W2156504548","https://openalex.org/W2736518123","https://openalex.org/W4205846253","https://openalex.org/W4253476163"],"related_works":["https://openalex.org/W3043929764","https://openalex.org/W2256848479","https://openalex.org/W2156504548","https://openalex.org/W2135860483","https://openalex.org/W4401131331","https://openalex.org/W4321180228","https://openalex.org/W2906389162","https://openalex.org/W4415530917","https://openalex.org/W2531124793","https://openalex.org/W7055805755"],"abstract_inverted_index":{"O":[0],"estudo":[1,89],"da":[2,34,85],"express\u00e3o":[3,125],"g\u00eanica":[4],"das":[5],"c\u00e9lulas":[6,24,65,72,98,139],"foliculares":[7,25,99,140],"pode":[8],"ser":[9,29],"a":[10,54,58,77,92,149],"chave":[11],"para":[12,43],"o":[13,74,95],"entendimento":[14],"dos":[15,126],"processos":[16],"de":[17,26,51,60,64,79,97,106,108,115,119,128,144],"crescimento":[18],"folicular,":[19,68],"ovula\u00e7\u00e3o":[20],"e":[21,81,94,117,134],"luteiniza\u00e7\u00e3o.":[22],"As":[23],"animais":[27],"podem":[28],"recuperadas":[30],"in":[31],"vivo":[32],"atrav\u00e9s":[33],"aspira\u00e7\u00e3o":[35],"folicular":[36],"(AF)":[37],"guiada":[38],"por":[39,71],"ultrassonografia,":[40],"uma":[41,61],"alternativa":[42],"n\u00e3o":[44],"submeter":[45],"as":[46],"f\u00eameas":[47],"ao":[48],"procedimento":[49],"cir\u00fargico":[50],"ovariectomia.":[52],"Entretanto,":[53],"AF":[55],"comumente":[56],"possibilita":[57],"recupera\u00e7\u00e3o":[59],"pequena":[62],"quantidade":[63],"em":[66],"fluido":[67],"geralmente":[69],"contaminado":[70],"sangu\u00edneas,":[73],"que":[75],"prejudica":[76],"extra\u00e7\u00e3o":[78],"RNA":[80,111,116],"interfere":[82],"na":[83,124],"qualidade":[84,121],"amostra.":[86],"No":[87],"presente":[88],"foram":[90,136],"comparadas":[91],"coleta":[93],"armazenamento":[96],"com":[100,122,142],"solu\u00e7\u00e3o":[101],"salina,":[102],"Trizol\u00ae,":[103],"dois":[104],"tamp\u00f5es":[105],"lise":[107,145],"eritr\u00f3citos":[109],"ou":[110],"later\u00ae.":[112],"Quantidades":[113],"adequadas":[114],"amostras":[118],"melhor":[120],"base":[123],"genes":[127],"refer\u00eancia":[129],"18S":[130],"rRNA,":[131],"GAPDH,":[132],"RPL19":[133],"PPIB":[135],"obtidas":[137],"nas":[138],"tratadas":[141],"tamp\u00e3o":[143],"celular":[146],"imediatamente":[147],"ap\u00f3s":[148],"coleta.":[150]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2022-05-12T00:00:00"}