{"id":"https://openalex.org/W4248288596","doi":"https://doi.org/10.21203/rs.3.rs-117842/v1","title":"Diaphragm Satellite Cells Isolation by Optimized MACS and the Effect of Mechanical Ventilation on their Proliferation and Differentiation Characteristics through MyoD and Myogenin Pathways","display_name":"Diaphragm Satellite Cells Isolation by Optimized MACS and the Effect of Mechanical Ventilation on their Proliferation and Differentiation Characteristics through MyoD and Myogenin Pathways","publication_year":2020,"publication_date":"2020-12-04","ids":{"openalex":"https://openalex.org/W4248288596","doi":"https://doi.org/10.21203/rs.3.rs-117842/v1"},"language":"en","primary_location":{"id":"doi:10.21203/rs.3.rs-117842/v1","doi":"https://doi.org/10.21203/rs.3.rs-117842/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-117842/v1","pdf_url":"https://www.researchsquare.com/article/rs-117842/v1.pdf?c=1631877541000","source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.researchsquare.com/article/rs-117842/v1.pdf?c=1631877541000","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5072148285","display_name":"Junying Ding","orcid":"https://orcid.org/0000-0003-1354-1343","observed_orcids":["https://orcid.org/0000-0003-1354-1343"]},"institutions":[{"id":"https://openalex.org/I4210148966","display_name":"Beijing Hospital of Traditional Chinese Medicine","ror":"https://ror.org/057vq6e26","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210148966"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Junying Ding","raw_affiliation_strings":["Beijing hospital of TCM"],"raw_orcid":"https://orcid.org/0000-0003-1354-1343","affiliations":[{"raw_affiliation_string":"Beijing hospital of TCM","institution_ids":["https://openalex.org/I4210148966"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100340539","display_name":"Qian Li","orcid":"https://orcid.org/0000-0001-7614-5618","observed_orcids":["https://orcid.org/0000-0001-7614-5618","https://orcid.org/0009-0004-6073-1670","https://orcid.org/0000-0002-9623-2716","https://orcid.org/0009-0007-2812-4901"]},"institutions":[{"id":"https://openalex.org/I2801766832","display_name":"McGill University Health Centre","ror":"https://ror.org/04cpxjv19","country_code":"CA","type":"healthcare","lineage":["https://openalex.org/I2801766832","https://openalex.org/I2801902503","https://openalex.org/I4210139015","https://openalex.org/I4405279980"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Qian Li","raw_affiliation_strings":["McGill University Health Centre"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"McGill University Health Centre","institution_ids":["https://openalex.org/I2801766832"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Feng Liang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2801766832","display_name":"McGill University Health Centre","ror":"https://ror.org/04cpxjv19","country_code":"CA","type":"healthcare","lineage":["https://openalex.org/I2801766832","https://openalex.org/I2801902503","https://openalex.org/I4210139015","https://openalex.org/I4405279980"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Feng Liang","raw_affiliation_strings":["McGill University Health Centre"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"McGill University Health Centre","institution_ids":["https://openalex.org/I2801766832"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5082823532","display_name":"Salyan Bhattarai","orcid":"https://orcid.org/0000-0002-6553-0286","observed_orcids":["https://orcid.org/0000-0002-6553-0286"]},"institutions":[{"id":"https://openalex.org/I2801766832","display_name":"McGill University Health Centre","ror":"https://ror.org/04cpxjv19","country_code":"CA","type":"healthcare","lineage":["https://openalex.org/I2801766832","https://openalex.org/I2801902503","https://openalex.org/I4210139015","https://openalex.org/I4405279980"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Salyan Bhattarai","raw_affiliation_strings":["McGill University Health Centre"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"McGill University Health Centre","institution_ids":["https://openalex.org/I2801766832"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5100750206","display_name":"Qingquan Liu","orcid":"https://orcid.org/0000-0002-0037-290X","observed_orcids":["https://orcid.org/0000-0002-0037-290X"]},"institutions":[{"id":"https://openalex.org/I4210148966","display_name":"Beijing Hospital of Traditional Chinese Medicine","ror":"https://ror.org/057vq6e26","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210148966"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Qingquan Liu","raw_affiliation_strings":["Beijing Hospital of Traditional Chinese Medicine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Beijing Hospital of Traditional Chinese Medicine","institution_ids":["https://openalex.org/I4210148966"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5072148285"],"corresponding_institution_ids":["https://openalex.org/I4210148966"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10441","display_name":"Muscle Physiology and Disorders","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10441","display_name":"Muscle Physiology and Disorders","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10322","display_name":"Respiratory Support and Mechanisms","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/2740","display_name":"Pulmonary and Respiratory Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10333","display_name":"Meat and Animal Product Quality","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/mechanical-ventilation","display_name":"mechanical ventilation","score":0.6809999942779541},{"id":"https://openalex.org/keywords/cell-isolation","display_name":"cell isolation","score":0.7480000257492065},{"id":"https://openalex.org/keywords/magnetic-activated-cell-sorting","display_name":"magnetic cell separation","score":0.8840000033378601},{"id":"https://openalex.org/keywords/cell-proliferation","display_name":"cell proliferation","score":0.8119999766349792},{"id":"https://openalex.org/keywords/myogenesis","display_name":"myogenesis","score":0.7910000085830688},{"id":"https://openalex.org/keywords/myod","display_name":"MyoD","score":0.996999979019165},{"id":"https://openalex.org/keywords/myogenin","display_name":"myogenin","score":0.9990000128746033},{"id":"https://openalex.org/keywords/skeletal-muscle-regeneration","display_name":"skeletal muscle regeneration","score":0.6909999847412109},{"id":"https://openalex.org/keywords/c57-mice","display_name":"C57 mice","score":0.8169999718666077}],"concepts":[{"id":"https://openalex.org/C97816823","wikidata":"https://www.wikidata.org/wiki/Q166726","display_name":"Myogenin","level":4,"score":0.8928830623626709},{"id":"https://openalex.org/C2778106681","wikidata":"https://www.wikidata.org/wiki/Q416095","display_name":"MyoD","level":4,"score":0.8076921105384827},{"id":"https://openalex.org/C19269812","wikidata":"https://www.wikidata.org/wiki/Q26540","display_name":"Satellite","level":2,"score":0.6220457553863525},{"id":"https://openalex.org/C2775941552","wikidata":"https://www.wikidata.org/wiki/Q25212305","display_name":"Isolation (microbiology)","level":2,"score":0.5590093731880188},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5293781757354736},{"id":"https://openalex.org/C164292776","wikidata":"https://www.wikidata.org/wiki/Q5271779","display_name":"Diaphragm (acoustics)","level":3,"score":0.49729278683662415},{"id":"https://openalex.org/C3763915","wikidata":"https://www.wikidata.org/wiki/Q3331437","display_name":"Myogenesis","level":3,"score":0.44261181354522705},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3793952465057373},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.260468065738678},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.23988750576972961},{"id":"https://openalex.org/C146978453","wikidata":"https://www.wikidata.org/wiki/Q3798668","display_name":"Aerospace engineering","level":1,"score":0.17033272981643677},{"id":"https://openalex.org/C60644358","wikidata":"https://www.wikidata.org/wiki/Q128570","display_name":"Bioinformatics","level":1,"score":0.10354945063591003},{"id":"https://openalex.org/C207200792","wikidata":"https://www.wikidata.org/wiki/Q428914","display_name":"Myocyte","level":2,"score":0.10346981883049011},{"id":"https://openalex.org/C119599485","wikidata":"https://www.wikidata.org/wiki/Q43035","display_name":"Electrical engineering","level":1,"score":0.09921601414680481},{"id":"https://openalex.org/C157138929","wikidata":"https://www.wikidata.org/wiki/Q570","display_name":"Loudspeaker","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.21203/rs.3.rs-117842/v1","doi":"https://doi.org/10.21203/rs.3.rs-117842/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-117842/v1","pdf_url":"https://www.researchsquare.com/article/rs-117842/v1.pdf?c=1631877541000","source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.21203/rs.3.rs-117842/v1","doi":"https://doi.org/10.21203/rs.3.rs-117842/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-117842/v1","pdf_url":"https://www.researchsquare.com/article/rs-117842/v1.pdf?c=1631877541000","source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.9476000070571899}],"study_designs":[],"awards":[{"id":"https://openalex.org/G2644209274","display_name":null,"funder_award_id":"7182071","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G952073967","display_name":null,"funder_award_id":"81503399","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"}],"funders":[{"id":"https://openalex.org/F4320310638","display_name":"McGill University","ror":"https://ror.org/01pxwe438"},{"id":"https://openalex.org/F4320314794","display_name":"McGill University Health Centre","ror":"https://ror.org/04cpxjv19"},{"id":"https://openalex.org/F4320321001","display_name":"National Natural Science Foundation of China","ror":"https://ror.org/01h0zpd94"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4248288596.pdf","grobid_xml":"https://content.openalex.org/works/W4248288596.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W3216688282","https://openalex.org/W4409256370","https://openalex.org/W2352032933","https://openalex.org/W2287726905","https://openalex.org/W2384144523","https://openalex.org/W3003936077","https://openalex.org/W4392014554","https://openalex.org/W4408735709","https://openalex.org/W2354732186","https://openalex.org/W2906351642"],"abstract_inverted_index":{"Abstract":[0],"Objective:":[1],"In":[2,206],"the":[3,24,31,40,48,51,90,110,116,127,170,184,194,224,235],"present":[4],"study,":[5],"we":[6],"aimed":[7],"to":[8,13,181,191,216,232],"establish":[9],"a":[10,152,165],"feasible":[11],"method":[12,212],"isolate":[14,217],"single":[15,58,142,166,218],"diaphragm":[16,36,59,143,174,219,228],"satellite":[17,37,60,144,175,220,229],"cells":[18,61,82,101,145,160,176,230],"from":[19,64,104,164,179,189],"C57":[20,65],"mice,":[21],"and":[22,33,54,109,118,131,198,234,239,252],"clarify":[23],"effect":[25],"of":[26,35,50,70,115,129,154,173,196,227,237],"mechanical":[27],"ventilation":[28],"(MV)":[29],"on":[30],"proliferation":[32,111,171],"differentiation":[34,91,113,185,225],"cells.":[38,221],"Moreover,":[39,193],"underlying":[41],"molecular":[42],"mechanism":[43],"was":[44,177,187,213],"also":[45],"explored.Methods:":[46],"After":[47,222],"dissection":[49],"diaphragm,":[52],"enzymolysis,":[53],"specific":[55],"antibody":[56,92],"selection,":[57],"were":[62,83,102,121,133,146,200,241],"harvested":[63,163],"mice":[66],"receiving":[67],"6":[68],"h":[69],"MV":[71,119],"or":[72,87,112],"not":[73],"with":[74,85,89],"optimized":[75,155],"magnetic-activated":[76],"cell":[77],"sorting":[78],"(MACS)":[79],"approach.":[80],"The":[81,100,141],"stained":[84],"BrdU":[86],"labeled":[88],"MYH3,":[93],"followed":[94],"by":[95,123,135],"observation":[96],"using":[97,151],"fluorescence":[98],"microscopy.":[99],"counted":[103],"randomly":[105],"selected":[106],"visual":[107],"fields,":[108],"characteristics":[114],"control":[117],"groups":[120],"compared":[122],"IMAGE":[124],"software.":[125],"Besides,":[126],"expressions":[128,195,236],"MyoD":[130,197,238],"myogenin":[132,199,240],"detected":[134],"quantitative":[136],"real-time":[137],"PCR":[138],"(qRT-PCR).":[139],"Results:":[140],"successfully":[147,214],"purified":[148],"through":[149],"MACS":[150],"set":[153],"parameters.":[156],"Generally":[157],"speaking,":[158],"1.5\u00d7105":[159],"could":[161],"be":[162],"diaphragm.":[167],"Upon":[168],"MV,":[169,223],"rate":[172,186,226],"decreased":[178],"88.74%":[180],"81.92%,":[182],"while":[183],"increased":[188],"17.94%":[190],"27.58%.":[192],"significantly":[201],"up-regulated":[202],"upon":[203],"MV.":[204],"Conclusions:":[205],"our":[207,244],"current":[208],"work,":[209],"an":[210],"efficient":[211],"established":[215],"tended":[231],"increase,":[233],"up-regulated.":[242],"Collectively,":[243],"findings":[245],"provided":[246],"valuable":[247],"insights":[248],"into":[249],"further":[250],"research":[251],"clinical":[253],"target":[254],"treatment.":[255]},"counts_by_year":[],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2022-05-12T00:00:00"}