{"id":"https://openalex.org/W4246065770","doi":"https://doi.org/10.1002/1098-2795(200011)57:3<290::aid-mrd11>3.3.co;2-r","title":"Porcine oocyte activation induced by a cytosolic sperm factor","display_name":"Porcine oocyte activation induced by a cytosolic sperm factor","publication_year":2000,"publication_date":"2000-11-01","ids":{"openalex":"https://openalex.org/W4246065770","doi":"https://doi.org/10.1002/1098-2795(200011)57:3<290::aid-mrd11>3.3.co;2-r"},"language":"en","primary_location":{"id":"doi:10.1002/1098-2795(200011)57:3<290::aid-mrd11>3.3.co;2-r","doi":"https://doi.org/10.1002/1098-2795(200011)57:3<290::aid-mrd11>3.3.co;2-r","is_oa":false,"landing_page_url":"https://doi.org/10.1002/1098-2795(200011)57:3<290::aid-mrd11>3.3.co;2-r","pdf_url":null,"source":{"id":"https://openalex.org/S125242520","display_name":"Molecular Reproduction and Development","issn_l":"1040-452X","issn":["1040-452X","1098-2795"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular Reproduction and Development","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5015759451","display_name":"Zolt\u00e1n Mach\u00e1ty","orcid":"https://orcid.org/0000-0001-9021-1706","observed_orcids":["https://orcid.org/0000-0001-9021-1706"]},"institutions":[{"id":"https://openalex.org/I76835614","display_name":"University of Missouri","ror":"https://ror.org/02ymw8z06","country_code":"US","type":"education","lineage":["https://openalex.org/I76835614"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Zolt\u00e1n Mach\u00e1ty","raw_affiliation_strings":["Department of Animal Sciences, University of Missouri-Columbia, Columbia, Missouri"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Animal Sciences, University of Missouri-Columbia, Columbia, Missouri","institution_ids":["https://openalex.org/I76835614"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5050849511","display_name":"Aaron Bonk","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I76835614","display_name":"University of Missouri","ror":"https://ror.org/02ymw8z06","country_code":"US","type":"education","lineage":["https://openalex.org/I76835614"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Aaron J. Bonk","raw_affiliation_strings":["Department of Animal Sciences, University of Missouri-Columbia, Columbia, Missouri"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Animal Sciences, University of Missouri-Columbia, Columbia, Missouri","institution_ids":["https://openalex.org/I76835614"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5018370978","display_name":"Birgit Ku\u0308hholzer","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I76835614","display_name":"University of Missouri","ror":"https://ror.org/02ymw8z06","country_code":"US","type":"education","lineage":["https://openalex.org/I76835614"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Birgit K\u00fchholzer","raw_affiliation_strings":["Department of Animal Sciences, University of Missouri-Columbia, Columbia, Missouri"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Animal Sciences, University of Missouri-Columbia, Columbia, Missouri","institution_ids":["https://openalex.org/I76835614"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5024184340","display_name":"Randall S. Prather","orcid":"https://orcid.org/0000-0002-6012-4035","observed_orcids":["https://orcid.org/0000-0002-6012-4035"]},"institutions":[{"id":"https://openalex.org/I76835614","display_name":"University of Missouri","ror":"https://ror.org/02ymw8z06","country_code":"US","type":"education","lineage":["https://openalex.org/I76835614"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Randall S. Prather","raw_affiliation_strings":["Department of Animal Sciences, University of Missouri-Columbia, Columbia, Missouri"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Animal Sciences, University of Missouri-Columbia, Columbia, Missouri","institution_ids":["https://openalex.org/I76835614"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5015759451"],"corresponding_institution_ids":["https://openalex.org/I76835614"],"apc_list":{"value":4260,"currency":"USD","value_usd":4260},"apc_paid":null,"fwci":0.3955,"has_fulltext":false,"cited_by_count":2,"citation_normalized_percentile":{"value":0.73840821,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"57","issue":"3","first_page":"290","last_page":"295"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10364","display_name":"Reproductive Biology and Fertility","score":0.9814000129699707,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10364","display_name":"Reproductive Biology and Fertility","score":0.9814000129699707,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10116","display_name":"Sperm and Testicular Function","score":0.006899999920278788,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10584","display_name":"Reproductive Physiology in Livestock","score":0.004399999976158142,"subfield":{"id":"https://openalex.org/subfields/1102","display_name":"Agronomy and Crop Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/oocyte-activation","display_name":"oocyte activation","score":0.859000027179718},{"id":"https://openalex.org/keywords/porcine-oocytes","display_name":"porcine oocytes","score":0.8199999928474426},{"id":"https://openalex.org/keywords/sperm-factor","display_name":"sperm factor","score":0.5849999785423279},{"id":"https://openalex.org/keywords/calcium-release","display_name":"calcium release","score":0.7599999904632568},{"id":"https://openalex.org/keywords/calcium-transients","display_name":"calcium transients","score":0.8330000042915344},{"id":"https://openalex.org/keywords/cortical-granule-exocytosis","display_name":"cortical granule exocytosis","score":0.9900000095367432},{"id":"https://openalex.org/keywords/pronucleus-formation","display_name":"pronuclear formation","score":0.9509999752044678},{"id":"https://openalex.org/keywords/meiotic-resumption","display_name":"meiotic resumption","score":0.7289999723434448},{"id":"https://openalex.org/keywords/early-cleavage","display_name":"early cleavage","score":0.4749999940395355},{"id":"https://openalex.org/keywords/blastocyst-development","display_name":"blastocyst development","score":0.8709999918937683},{"id":"https://openalex.org/keywords/sperm-oocyte-fusion","display_name":"sperm-oocyte fusion","score":0.550000011920929},{"id":"https://openalex.org/keywords/22-kda-protein","display_name":"22 kDa protein","score":0.6840000152587891}],"concepts":[{"id":"https://openalex.org/C179265757","wikidata":"https://www.wikidata.org/wiki/Q7095247","display_name":"Oocyte activation","level":4,"score":0.8913270235061646},{"id":"https://openalex.org/C110758877","wikidata":"https://www.wikidata.org/wiki/Q2614103","display_name":"Pronucleus","level":5,"score":0.8850281238555908},{"id":"https://openalex.org/C2776690073","wikidata":"https://www.wikidata.org/wiki/Q3246226","display_name":"Oocyte","level":3,"score":0.813371479511261},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7721846103668213},{"id":"https://openalex.org/C2781087480","wikidata":"https://www.wikidata.org/wiki/Q17145","display_name":"Sperm","level":2,"score":0.7083508968353271},{"id":"https://openalex.org/C88972607","wikidata":"https://www.wikidata.org/wiki/Q2666904","display_name":"Human fertilization","level":2,"score":0.6280309557914734},{"id":"https://openalex.org/C2776803348","wikidata":"https://www.wikidata.org/wiki/Q211050","display_name":"Gamete","level":3,"score":0.6274729371070862},{"id":"https://openalex.org/C6902291","wikidata":"https://www.wikidata.org/wiki/Q417889","display_name":"Maturation promoting factor","level":5,"score":0.6249019503593445},{"id":"https://openalex.org/C16685009","wikidata":"https://www.wikidata.org/wiki/Q335057","display_name":"Andrology","level":1,"score":0.6228962540626526},{"id":"https://openalex.org/C2778177303","wikidata":"https://www.wikidata.org/wiki/Q227717","display_name":"Blastocyst","level":4,"score":0.5814871191978455},{"id":"https://openalex.org/C90642696","wikidata":"https://www.wikidata.org/wiki/Q1759519","display_name":"Polyspermy","level":4,"score":0.4228544235229492},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.39999544620513916},{"id":"https://openalex.org/C28748434","wikidata":"https://www.wikidata.org/wiki/Q170145","display_name":"Zygote","level":4,"score":0.3118067681789398},{"id":"https://openalex.org/C105702510","wikidata":"https://www.wikidata.org/wiki/Q514","display_name":"Anatomy","level":1,"score":0.22224220633506775},{"id":"https://openalex.org/C87073359","wikidata":"https://www.wikidata.org/wiki/Q33196","display_name":"Embryogenesis","level":3,"score":0.18244019150733948},{"id":"https://openalex.org/C196843134","wikidata":"https://www.wikidata.org/wiki/Q33196","display_name":"Embryo","level":2,"score":0.15018734335899353},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.13459959626197815},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.09611979126930237},{"id":"https://openalex.org/C29537977","wikidata":"https://www.wikidata.org/wiki/Q188941","display_name":"Cell cycle","level":3,"score":0.05627790093421936},{"id":"https://openalex.org/C120504264","wikidata":"https://www.wikidata.org/wiki/Q1147372","display_name":"Cyclin-dependent kinase 1","level":4,"score":0.0},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1002/1098-2795(200011)57:3<290::aid-mrd11>3.3.co;2-r","doi":"https://doi.org/10.1002/1098-2795(200011)57:3<290::aid-mrd11>3.3.co;2-r","is_oa":false,"landing_page_url":"https://doi.org/10.1002/1098-2795(200011)57:3<290::aid-mrd11>3.3.co;2-r","pdf_url":null,"source":{"id":"https://openalex.org/S125242520","display_name":"Molecular Reproduction and Development","issn_l":"1040-452X","issn":["1040-452X","1098-2795"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular Reproduction and Development","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2151190823","https://openalex.org/W2063183173","https://openalex.org/W2128480501","https://openalex.org/W2091080010","https://openalex.org/W2061064038","https://openalex.org/W134799949","https://openalex.org/W267016373","https://openalex.org/W2155379199","https://openalex.org/W2159943654","https://openalex.org/W2132286825"],"abstract_inverted_index":{"It":[0],"is":[1],"not":[2,167],"known":[3],"how":[4],"the":[5,9,14,30,55,82,86,91,115,122,136,148,163,175,187],"fertilizing":[6],"sperm":[7,27,48,92,176],"elicits":[8],"release":[10,32],"of":[11,74,81,90,114,124,162],"Ca2+":[12,31,58,69,87],"from":[13,25],"oocyte's":[15,188],"intracellular":[16,56],"stores.":[17],"We":[18],"investigated":[19],"whether":[20],"a":[21,125,183],"crude":[22],"extract":[23,49,83],"isolated":[24],"boar":[26],"could":[28,166],"induce":[29],"and":[33,37,104,143],"trigger":[34,168],"subsequent":[35],"early":[36],"late":[38],"activation":[39],"events":[40],"upon":[41],"injection":[42],"into":[43,186],"matured":[44],"porcine":[45,179],"oocytes.":[46,76],"The":[47,94,171],"induced":[50],"an":[51],"immediate":[52],"rise":[53],"in":[54,60,71,111,157],"free":[57],"concentration":[59],"all":[61],"oocytes":[62,96,139,180],"tested,":[63],"which":[64],"was":[65,119],"followed":[66],"by":[67,121,181],"repetitive":[68],"transients":[70],"11":[72],"out":[73],"14":[75],"Heat":[77],"or":[78],"trypsin":[79],"treatment":[80],"totally":[84],"abolished":[85],"releasing":[88],"activity":[89],"factor.":[93],"injected":[95,138],"showed":[97],"cortical":[98],"granule":[99],"exocytosis,":[100],"they":[101],"resumed":[102],"meiosis":[103],"entered":[105],"first":[106],"interphase:":[107],"pronuclei":[108],"were":[109],"formed":[110],"89.2%":[112],"(132/148)":[113],"cases.":[116],"Pronuclear":[117],"formation":[118],"accompanied":[120],"appearance":[123],"new":[126],"22":[127],"kDa":[128],"protein":[129],"as":[130],"normally":[131],"seen":[132],"at":[133],"fertilization.":[134],"Of":[135],"successfully":[137],"51.7%":[140],"(105/203)":[141],"cleaved":[142],"2.0%":[144],"(4/203)":[145],"developed":[146],"to":[147],"blastocyst":[149],"stage":[150],"after":[151,190],"being":[152],"cultured":[153],"for":[154],"7":[155],"days":[156],"NCSU":[158],"23":[159],"medium.":[160],"Injection":[161],"carrier":[164],"medium":[165],"these":[169],"changes.":[170],"results":[172],"indicate":[173],"that":[174],"might":[177],"activate":[178],"introducing":[182],"soluble":[184],"factor":[185],"cytoplasm":[189],"gamete":[191],"fusion.":[192],"Mol.":[193],"Reprod.":[194],"Dev.":[195],"57:290\u2013295,":[196],"2000.":[197],"\u00a9":[198],"2000":[199],"Wiley-Liss,":[200],"Inc.":[201]},"counts_by_year":[],"updated_date":"2026-09-22T06:50:58.402377","created_date":"2022-05-12T00:00:00"}