{"id":"https://openalex.org/W4244514930","doi":"https://doi.org/10.1007/978-1-4612-0119-9_28","title":"Characterization of Heterologous and Native Enzyme Activity Profiles in Metabolically Engineered Zymomonas Mobilis Strains During Batch Fermentation of Glucose and Xylose Mixtures","display_name":"Characterization of Heterologous and Native Enzyme Activity Profiles in Metabolically Engineered Zymomonas Mobilis Strains During Batch Fermentation of Glucose and Xylose Mixtures","publication_year":2002,"publication_date":"2002-01-01","ids":{"openalex":"https://openalex.org/W4244514930","doi":"https://doi.org/10.1007/978-1-4612-0119-9_28"},"language":"en","primary_location":{"id":"doi:10.1007/978-1-4612-0119-9_28","doi":"https://doi.org/10.1007/978-1-4612-0119-9_28","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-4612-0119-9_28","pdf_url":null,"source":{"id":"https://openalex.org/S4306463399","display_name":"Humana Press eBooks","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310315801","host_organization_name":"Humana Press","host_organization_lineage":["https://openalex.org/P4310315801","https://openalex.org/P4310319900"],"host_organization_lineage_names":["Humana Press","Springer Science+Business Media"],"type":"ebook platform"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology for Fuels and Chemicals","raw_type":"book-chapter"},"volume":{"id":"https://openalex.org/W2287688460","display_name":"Biotechnology for Fuels and Chemicals"},"type":"book-chapter","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5017203537","display_name":"Qiang Gao","orcid":"https://orcid.org/0000-0003-0490-0690","observed_orcids":["https://orcid.org/0000-0003-0490-0690"]},"institutions":[{"id":"https://openalex.org/I188538660","display_name":"University of Colorado Boulder","ror":"https://ror.org/02ttsq026","country_code":"US","type":"education","lineage":["https://openalex.org/I188538660"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Qiang Gao","raw_affiliation_strings":["Department of Chemical Engineering, University of Colorado, 80309-0424, Boulder, CO, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical Engineering, University of Colorado, 80309-0424, Boulder, CO, USA","institution_ids":["https://openalex.org/I188538660"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100402962","display_name":"Min Zhang","orcid":"https://orcid.org/0000-0002-9015-2184","observed_orcids":["https://orcid.org/0000-0002-9015-2184"]},"institutions":[{"id":"https://openalex.org/I1297288678","display_name":"National Laboratory of the Rockies","ror":"https://ror.org/036266993","country_code":"US","type":"facility","lineage":["https://openalex.org/I1297288678","https://openalex.org/I1330989302","https://openalex.org/I2800842121"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Min Zhang","raw_affiliation_strings":["Biotechnology Division for Fuels and Chemicals, National Renewable Energy Laboratory, 80401-3933, Golden, CO, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biotechnology Division for Fuels and Chemicals, National Renewable Energy Laboratory, 80401-3933, Golden, CO, USA","institution_ids":["https://openalex.org/I1297288678"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5013871933","display_name":"James D. McMillan","orcid":"https://orcid.org/0000-0001-5143-9978","observed_orcids":["https://orcid.org/0000-0001-5143-9978"]},"institutions":[{"id":"https://openalex.org/I1297288678","display_name":"National Laboratory of the Rockies","ror":"https://ror.org/036266993","country_code":"US","type":"facility","lineage":["https://openalex.org/I1297288678","https://openalex.org/I1330989302","https://openalex.org/I2800842121"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"James D. McMillan","raw_affiliation_strings":["Biotechnology Division for Fuels and Chemicals, National Renewable Energy Laboratory, 80401-3933, Golden, CO, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biotechnology Division for Fuels and Chemicals, National Renewable Energy Laboratory, 80401-3933, Golden, CO, USA","institution_ids":["https://openalex.org/I1297288678"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5075981089","display_name":"Dhinakar S. Kompala","orcid":"https://orcid.org/0000-0002-9617-1036","observed_orcids":["https://orcid.org/0000-0002-9617-1036"]},"institutions":[{"id":"https://openalex.org/I188538660","display_name":"University of Colorado Boulder","ror":"https://ror.org/02ttsq026","country_code":"US","type":"education","lineage":["https://openalex.org/I188538660"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Dhinakar S. Kompala","raw_affiliation_strings":["Department of Chemical Engineering, University of Colorado, 80309-0424, Boulder, CO, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical Engineering, University of Colorado, 80309-0424, Boulder, CO, USA","institution_ids":["https://openalex.org/I188538660"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5075981089"],"corresponding_institution_ids":["https://openalex.org/I188538660"],"apc_list":null,"apc_paid":null,"fwci":0.3449,"has_fulltext":false,"cited_by_count":2,"citation_normalized_percentile":{"value":0.53657126,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":"341","last_page":"355"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.9945999979972839,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.9945999979972839,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10171","display_name":"Biofuel production and bioconversion","score":0.004399999976158142,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12688","display_name":"Microbial metabolism and enzyme function","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/zymomonas-mobilis","display_name":"Zymomonas mobilis","score":1.0},{"id":"https://openalex.org/keywords/xylose-fermentation","display_name":"xylose fermentation","score":0.9490000009536743},{"id":"https://openalex.org/keywords/d-xylose","display_name":"D-xylose","score":0.7720000147819519},{"id":"https://openalex.org/keywords/l-arabinose","display_name":"L-arabinose","score":0.9390000104904175},{"id":"https://openalex.org/keywords/pentose-phosphate-pathway","display_name":"pentose phosphate pathway","score":0.9929999709129333},{"id":"https://openalex.org/keywords/glycolytic-enzymes","display_name":"glycolytic enzymes","score":0.746999979019165},{"id":"https://openalex.org/keywords/heterologous-enzyme-expression","display_name":"heterologous enzyme expression","score":0.6850000023841858},{"id":"https://openalex.org/keywords/glucose-xylose-mixtures","display_name":"glucose-xylose mixtures","score":0.8169999718666077},{"id":"https://openalex.org/keywords/metabolic-bottlenecks","display_name":"metabolic bottlenecks","score":0.7089999914169312},{"id":"https://openalex.org/keywords/batch-fermentation","display_name":"batch fermentation","score":0.4959999918937683}],"concepts":[{"id":"https://openalex.org/C2780604136","wikidata":"https://www.wikidata.org/wiki/Q245744","display_name":"Zymomonas mobilis","level":4,"score":0.9649913311004639},{"id":"https://openalex.org/C2780294150","wikidata":"https://www.wikidata.org/wiki/Q66589603","display_name":"Xylose","level":3,"score":0.9209353923797607},{"id":"https://openalex.org/C2780261296","wikidata":"https://www.wikidata.org/wiki/Q205654","display_name":"Pentose","level":3,"score":0.7988618612289429},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.7621318101882935},{"id":"https://openalex.org/C2781135521","wikidata":"https://www.wikidata.org/wiki/Q8045523","display_name":"Xylose metabolism","level":4,"score":0.7506861090660095},{"id":"https://openalex.org/C100544194","wikidata":"https://www.wikidata.org/wiki/Q41760","display_name":"Fermentation","level":2,"score":0.6698709726333618},{"id":"https://openalex.org/C3409486","wikidata":"https://www.wikidata.org/wiki/Q827489","display_name":"Pentose phosphate pathway","level":4,"score":0.5928159952163696},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.47131070494651794},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.44466251134872437},{"id":"https://openalex.org/C192989942","wikidata":"https://www.wikidata.org/wiki/Q68685","display_name":"Metabolic pathway","level":3,"score":0.4255527853965759},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.42074206471443176},{"id":"https://openalex.org/C2779277513","wikidata":"https://www.wikidata.org/wiki/Q8045529","display_name":"Xylose isomerase","level":4,"score":0.41218143701553345},{"id":"https://openalex.org/C20251656","wikidata":"https://www.wikidata.org/wiki/Q162643","display_name":"Glycolysis","level":3,"score":0.20310521125793457},{"id":"https://openalex.org/C65183824","wikidata":"https://www.wikidata.org/wiki/Q194361","display_name":"Ethanol fuel","level":3,"score":0.0848034918308258}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1007/978-1-4612-0119-9_28","doi":"https://doi.org/10.1007/978-1-4612-0119-9_28","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-4612-0119-9_28","pdf_url":null,"source":{"id":"https://openalex.org/S4306463399","display_name":"Humana Press eBooks","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310315801","host_organization_name":"Humana Press","host_organization_lineage":["https://openalex.org/P4310315801","https://openalex.org/P4310319900"],"host_organization_lineage_names":["Humana Press","Springer Science+Business Media"],"type":"ebook platform"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology for Fuels and Chemicals","raw_type":"book-chapter"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":11,"referenced_works":["https://openalex.org/W1493787054","https://openalex.org/W1984409837","https://openalex.org/W1990433965","https://openalex.org/W1992993824","https://openalex.org/W2013947082","https://openalex.org/W2024424485","https://openalex.org/W2026362025","https://openalex.org/W2073451692","https://openalex.org/W2108985830","https://openalex.org/W4232337218","https://openalex.org/W4307612972"],"related_works":["https://openalex.org/W2024424485","https://openalex.org/W2745272842","https://openalex.org/W2096935275","https://openalex.org/W1861195345","https://openalex.org/W7064828366","https://openalex.org/W1979934096","https://openalex.org/W4409583364","https://openalex.org/W145624591","https://openalex.org/W3119842251","https://openalex.org/W4299410414"],"abstract_inverted_index":{"Zymomonas":[0],"mobilis":[1,23,84],"has":[2],"been":[3,34],"metabolically":[4],"engineered":[5,135],"to":[6,12,101],"broaden":[7],"its":[8],"substrate":[9],"utilization":[10,44],"range":[11],"include":[13],"D-xylose":[14,32],"and":[15,20,48,99,118,121,138],"L-arabinose.":[16],"Both":[17],"genomically":[18],"integrated":[19],"plasmid-bearing":[21],"Z.":[22,83],"strains":[24,87],"that":[25],"are":[26],"capable":[27],"of":[28,62,74,111,146],"fermenting":[29],"the":[30,60,63,109,116,130,134,144],"pentose":[31,52],"have":[33,58],"created":[35],"by":[36],"incorporating":[37],"four":[38,64],"genes:":[39],"two":[40,49,80],"genes":[41,50],"encoding":[42,51],"xylose":[43,69,117,147],"metabolic":[45,136],"enzymes":[46,55,67,114],"(xylA/xylB)":[47],"phosphate":[53],"pathway":[54],"(talB/tktA).":[56],"We":[57],"characterized":[59],"activities":[61,110],"newly":[65],"introduced":[66],"for":[68,141],"metabolism,":[70],"along":[71],"with":[72],"those":[73],"three":[75],"native":[76],"glycolytic":[77],"enzymes,":[78],"in":[79,93,115,133],"different":[81],"xylose-fermenting":[82],"strains.":[85],"These":[86,124],"were":[88,97],"grown":[89],"on":[90,129],"glucose\u2014xylose":[91],"mixtures":[92],"computer-controlled":[94],"fermentors.":[95],"Samples":[96],"collected":[98],"analyzed":[100],"determine":[102],"extracellular":[103],"metabolite":[104],"concentrations":[105],"as":[106,108],"well":[107],"several":[112],"intracellular":[113],"glucose":[119],"uptake":[120],"catabolism":[122],"pathways.":[123],"measurements":[125],"provide":[126],"new":[127],"insights":[128],"possible":[131],"bottle-necks":[132],"pathways":[137],"suggest":[139],"methods":[140],"further":[142],"improving":[143],"efficiency":[145],"fermentation.":[148]},"counts_by_year":[],"updated_date":"2026-10-10T09:11:00.788812","created_date":"2022-05-12T00:00:00"}