{"id":"https://openalex.org/W4244353264","doi":"https://doi.org/10.1385/1-59259-136-1:297","title":"Differential Display","display_name":"Differential Display","publication_year":2003,"publication_date":"2003-11-15","ids":{"openalex":"https://openalex.org/W4244353264","doi":"https://doi.org/10.1385/1-59259-136-1:297","pmid":"https://pubmed.ncbi.nlm.nih.gov/21340906"},"language":"en","primary_location":{"id":"doi:10.1385/1-59259-136-1:297","doi":"https://doi.org/10.1385/1-59259-136-1:297","is_oa":false,"landing_page_url":"https://doi.org/10.1385/1-59259-136-1:297","pdf_url":null,"source":{"id":"https://openalex.org/S4306463399","display_name":"Humana Press eBooks","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310315801","host_organization_name":"Humana Press","host_organization_lineage":["https://openalex.org/P4310315801","https://openalex.org/P4310319900"],"host_organization_lineage_names":["Humana Press","Springer Science+Business Media"],"type":"ebook platform"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Metastasis Research Protocols","raw_type":"book-chapter"},"volume":{"id":"https://openalex.org/W4231144230","display_name":"Metastasis Research Protocols"},"type":"book-chapter","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5108352771","display_name":"Ruey Ho Kao","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1324925910","display_name":"St Thomas' Hospital","ror":"https://ror.org/054gk2851","country_code":"GB","type":"healthcare","lineage":["https://openalex.org/I1324925910","https://openalex.org/I200166805","https://openalex.org/I4210111135"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Ruey Ho Kao","raw_affiliation_strings":["Richard Dimbleby Department of Cancer Research/ICRF Laboratory, Rayne Institute, St. Thomas Hospital, London, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Richard Dimbleby Department of Cancer Research/ICRF Laboratory, Rayne Institute, St. Thomas Hospital, London, UK","institution_ids":["https://openalex.org/I1324925910"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5075999310","display_name":"Giulio Francia","orcid":"https://orcid.org/0000-0002-3752-3070","observed_orcids":["https://orcid.org/0000-0002-3752-3070"]},"institutions":[{"id":"https://openalex.org/I1324925910","display_name":"St Thomas' Hospital","ror":"https://ror.org/054gk2851","country_code":"GB","type":"healthcare","lineage":["https://openalex.org/I1324925910","https://openalex.org/I200166805","https://openalex.org/I4210111135"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Giulio Francia","raw_affiliation_strings":["Richard Dimbleby Department of Cancer Research/ICRF Laboratory, Rayne Institute, St. Thomas Hospital, London, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Richard Dimbleby Department of Cancer Research/ICRF Laboratory, Rayne Institute, St. Thomas Hospital, London, UK","institution_ids":["https://openalex.org/I1324925910"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I1324925910"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":{"value":0.45609,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"57","issue":null,"first_page":"297","last_page":"305"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.8256999850273132,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.8256999850273132,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10885","display_name":"Gene expression and cancer classification","score":0.16419999301433563,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10604","display_name":"RNA Research and Splicing","score":0.0038999998942017555,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/differential-display","display_name":"differential display","score":1.0},{"id":"https://openalex.org/keywords/cancer-genetics","display_name":"cancer genetics","score":0.746999979019165},{"id":"https://openalex.org/keywords/gene-expression-profiling","display_name":"gene expression profiling","score":0.6819999814033508},{"id":"https://openalex.org/keywords/subtractive-hybridization","display_name":"subtractive hybridization","score":0.8529999852180481},{"id":"https://openalex.org/keywords/cdna-synthesis","display_name":"cDNA synthesis","score":0.46299999952316284},{"id":"https://openalex.org/keywords/differential-gene-expression","display_name":"differential gene expression","score":0.5929999947547913},{"id":"https://openalex.org/keywords/tumor-evolution","display_name":"tumor evolution","score":0.5490000247955322}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6824696063995361},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.6634998917579651},{"id":"https://openalex.org/C2777563447","wikidata":"https://www.wikidata.org/wiki/Q7243397","display_name":"Primer (cosmetics)","level":2,"score":0.6268472075462341},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.566909670829773},{"id":"https://openalex.org/C196436469","wikidata":"https://www.wikidata.org/wiki/Q6587700","display_name":"Suppression subtractive hybridization","level":5,"score":0.5549566745758057},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.5469813346862793},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5236532688140869},{"id":"https://openalex.org/C2776657240","wikidata":"https://www.wikidata.org/wiki/Q5275341","display_name":"Differential display","level":4,"score":0.4583929777145386},{"id":"https://openalex.org/C156719811","wikidata":"https://www.wikidata.org/wiki/Q28774","display_name":"Reverse transcriptase","level":4,"score":0.4482635259628296},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.4456692636013031},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.339526891708374},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.08431762456893921},{"id":"https://openalex.org/C189482833","wikidata":"https://www.wikidata.org/wiki/Q869146","display_name":"cDNA library","level":4,"score":0.06477078795433044},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1385/1-59259-136-1:297","doi":"https://doi.org/10.1385/1-59259-136-1:297","is_oa":false,"landing_page_url":"https://doi.org/10.1385/1-59259-136-1:297","pdf_url":null,"source":{"id":"https://openalex.org/S4306463399","display_name":"Humana Press eBooks","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310315801","host_organization_name":"Humana Press","host_organization_lineage":["https://openalex.org/P4310315801","https://openalex.org/P4310319900"],"host_organization_lineage_names":["Humana Press","Springer Science+Business Media"],"type":"ebook platform"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Metastasis Research Protocols","raw_type":"book-chapter"},{"id":"pmid:21340906","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/21340906","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in molecular medicine","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.46810001134872437}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2067434616","https://openalex.org/W4388384725","https://openalex.org/W14234014","https://openalex.org/W4281728242","https://openalex.org/W2063771912","https://openalex.org/W2395882998","https://openalex.org/W2019903076","https://openalex.org/W2145274484","https://openalex.org/W2412632843","https://openalex.org/W73254301"],"abstract_inverted_index":{"The":[0,187,282],"study":[1],"of":[2,40,76,79,83,106,114,125,157,166,181,190,193,252,295],"cancer":[3,134],"genetics":[4,129],"has":[5,57,88,96],"focused":[6],"primarily,":[7],"until":[8],"now,":[9],"on":[10,210],"changes":[11],"at":[12,52],"the":[13,26,38,53,72,80,112,122,133,153,164,167,179,197,253,270],"genomic":[14],"level.":[15],"However,":[16],"activating,":[17],"or":[18,221],"inactivating,":[19],"mutations/deletions/rearran":[20],"ments":[21],"in":[22,29,32,62,71,91,127,132,139,178,255],"DNA":[23],"are":[24],"not":[25,130],"only":[27,131],"way":[28],"which":[30,43],"alterations":[31,61],"gene":[33,64,107],"expression":[34,65,108,128],"can":[35,203,263,285],"occur.":[36],"Since":[37],"development":[39],"subtractive":[41],"hybridization,":[42],"screens":[44],"for":[45,276],"differentially":[46,216],"expressed":[47,217],"genes":[48],"between":[49],"related":[50],"populations":[51],"RNA":[54],"level,":[55],"evidence":[56],"accrued":[58],"showing":[59],"that":[60,95],"downstream":[63],"might":[66],"play":[67],"a":[68,172,182,211,240,250],"significant":[69],"role":[70],"biology":[73,135],"and":[74,118,147,267,272,279],"evolution":[75],"cancer.":[77],"Establishment":[78],"novel":[81,150],"technique":[82,151,284],"differential":[84,296],"display":[85,297],"(DD)":[86],"1,2(1,2)":[87],"facilitated":[89],"studies":[90],"this":[92],"new":[93],"field":[94,136],"been":[97],"termed":[98],"\"expression":[99],"genetics\"":[100],"3).":[101],"Compared":[102],"with":[103,159],"traditional":[104],"methods":[105],"analysis":[109],"DD":[110,283],"possesses":[111],"advantages":[113],"simplicity,":[115],"sensitivity,":[116],"reproducibility,":[117],"speed.":[119],"It":[120],"is":[121],"current":[123],"method":[124],"choice":[126],"but":[137],"also":[138],"such":[140],"diverse":[141],"areas":[142],"as":[143],"embryology,":[144],"developmental":[145],"biology,":[146],"neurobiology.":[148],"This":[149],"involves":[152],"reverse":[154],"transcription":[155],"(RT)":[156],"mRNAs":[158,194],"oligo-dT":[160],"primers":[161],"anchored":[162],"to":[163,214,223],"beginning":[165],"poly(A)":[168],"tail,":[169],"followed":[170],"by":[171,208],"poly":[173],"merase":[174],"chain":[175],"reaction":[176],"(PCR)":[177],"presence":[180],"second":[183],"arbitrary":[184],"random":[185],"primer.":[186],"amplified":[188],"cDNAs":[189],"3'":[191],"termini":[192],"obtained":[195],"(with":[196],"same":[198],"primers)":[199],"from":[200,239,269],"different":[201],"samples":[202],"then":[204],"be":[205,224,244,265,286],"run":[206],"side":[207,209],"sequencing":[212],"gel":[213,271],"allow":[215],"genes,":[218],"either":[219],"upregulated":[220],"downregulated,":[222],"visualized":[225],"(see":[226],"Fig.":[227],"1).":[228],"By":[229],"changing":[230],"primer":[231],"pair":[232],"combinations":[233],"approx":[234],"15,000":[235],"individual":[236],"mRNA":[237,254],"species":[238],"mammalian":[241],"cell":[242,258],"may":[243],"analyzed":[245],"reasonably":[246],"quickly,":[247],"thus":[248],"providing":[249],"\"fingerprint\"":[251],"any":[256],"particular":[257],"type.":[259],"Differential":[260],"cDNA":[261],"fragments":[262],"easily":[264],"recovered":[266],"reamplified":[268],"cloned":[273],"into":[274,288],"vectors":[275],"future":[277],"screening":[278],"characterization":[280],"work.":[281],"subdivided":[287],"five":[289],"steps":[290],"fig.":[291],"1.":[292],"Principal":[293],"procedures":[294],"technique.":[298]},"counts_by_year":[{"year":2021,"cited_by_count":1}],"updated_date":"2026-10-10T09:11:00.788812","created_date":"2022-05-12T00:00:00"}
