{"id":"https://openalex.org/W4240964296","doi":"https://doi.org/10.1042/ba20080015","title":"Generation of an affinity matrix useful in the purification of natural inhibitors of plasmepsin II, an antimalarial\u2010drug target","display_name":"Generation of an affinity matrix useful in the purification of natural inhibitors of plasmepsin II, an antimalarial\u2010drug target","publication_year":2009,"publication_date":"2009-01-06","ids":{"openalex":"https://openalex.org/W4240964296","doi":"https://doi.org/10.1042/ba20080015","pmid":"https://pubmed.ncbi.nlm.nih.gov/18412540"},"language":"en","primary_location":{"id":"doi:10.1042/ba20080015","doi":"https://doi.org/10.1042/ba20080015","is_oa":false,"landing_page_url":"https://doi.org/10.1042/ba20080015","pdf_url":null,"source":{"id":"https://openalex.org/S34872747","display_name":"Biotechnology and Applied Biochemistry","issn_l":"0885-4513","issn":["0885-4513","1470-8744"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and Applied Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5094072847","display_name":"Angel Rafael Ramirez-Ramirez","orcid":"https://orcid.org/0009-0001-4216-5925","observed_orcids":["https://orcid.org/0009-0001-4216-5925"]},"institutions":[{"id":"https://openalex.org/I2656126","display_name":"University of Havana","ror":"https://ror.org/04204gr61","country_code":"CU","type":"education","lineage":["https://openalex.org/I2656126"]},{"id":"https://openalex.org/I4210148836","display_name":"Universidad de Guant\u00e1namo","ror":"https://ror.org/047d13a24","country_code":"CU","type":"education","lineage":["https://openalex.org/I4210148836"]}],"countries":["CU"],"is_corresponding":true,"raw_author_name":"Angel\u00a0R. Ram\u00edrez","raw_affiliation_strings":["2 Present address: Facultad Agroforestal de Montana, Centro Universitario de Guanta\u0301namo, Guanta\u0301namo, Cuba","Centro de Estudio de Protei\u0301nas, Facultad de Biologi\u0301a, Universidad de la Habana, Calle 25 #455 entre J e I, Vedado, CP 10400, La Habana, Cuba","2Present address: Facultad Agroforestal de Montana, Centro Universitario de Guant\u00e1namo, Guant\u00e1namo, Cuba","Centro de Estudio de Prote\u00ednas, Facultad de Biolog\u00eda, Universidad de la Habana, Calle 25 #455 entre J e I, Vedado, CP 10400, La Habana, Cuba"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"2 Present address: Facultad Agroforestal de Montana, Centro Universitario de Guanta\u0301namo, Guanta\u0301namo, Cuba","institution_ids":["https://openalex.org/I4210148836"]},{"raw_affiliation_string":"Centro de Estudio de Protei\u0301nas, Facultad de Biologi\u0301a, Universidad de la Habana, Calle 25 #455 entre J e I, Vedado, CP 10400, La Habana, Cuba","institution_ids":["https://openalex.org/I2656126"]},{"raw_affiliation_string":"2Present address: Facultad Agroforestal de Montana, Centro Universitario de Guant\u00e1namo, Guant\u00e1namo, Cuba","institution_ids":["https://openalex.org/I4210148836"]},{"raw_affiliation_string":"Centro de Estudio de Prote\u00ednas, Facultad de Biolog\u00eda, Universidad de la Habana, Calle 25 #455 entre J e I, Vedado, CP 10400, La Habana, Cuba","institution_ids":["https://openalex.org/I2656126"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5023399677","display_name":"Yasel Guerra","orcid":"https://orcid.org/0000-0002-7256-6885","observed_orcids":["https://orcid.org/0000-0002-7256-6885"]},"institutions":[{"id":"https://openalex.org/I2656126","display_name":"University of Havana","ror":"https://ror.org/04204gr61","country_code":"CU","type":"education","lineage":["https://openalex.org/I2656126"]}],"countries":["CU"],"is_corresponding":false,"raw_author_name":"Yasel Guerra","raw_affiliation_strings":["Centro de Estudio de Protei\u0301nas, Facultad de Biologi\u0301a, Universidad de la Habana, Calle 25 #455 entre J e I, Vedado, CP 10400, La Habana, Cuba","Centro de Estudio de Prote\u00ednas, Facultad de Biolog\u00eda, Universidad de la Habana, Calle 25 #455 entre J e I, Vedado, CP 10400, La Habana, Cuba"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centro de Estudio de Protei\u0301nas, Facultad de Biologi\u0301a, Universidad de la Habana, Calle 25 #455 entre J e I, Vedado, CP 10400, La Habana, Cuba","institution_ids":["https://openalex.org/I2656126"]},{"raw_affiliation_string":"Centro de Estudio de Prote\u00ednas, Facultad de Biolog\u00eda, Universidad de la Habana, Calle 25 #455 entre J e I, Vedado, CP 10400, La Habana, Cuba","institution_ids":["https://openalex.org/I2656126"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111791430","display_name":"Anabel Otero","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2656126","display_name":"University of Havana","ror":"https://ror.org/04204gr61","country_code":"CU","type":"education","lineage":["https://openalex.org/I2656126"]}],"countries":["CU"],"is_corresponding":false,"raw_author_name":"Anabel Otero","raw_affiliation_strings":["Centro de Estudio de Protei\u0301nas, Facultad de Biologi\u0301a, Universidad de la Habana, Calle 25 #455 entre J e I, Vedado, CP 10400, La Habana, Cuba","Centro de Estudio de Prote\u00ednas, Facultad de Biolog\u00eda, Universidad de la Habana, Calle 25 #455 entre J e I, Vedado, CP 10400, La Habana, Cuba"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centro de Estudio de Protei\u0301nas, Facultad de Biologi\u0301a, Universidad de la Habana, Calle 25 #455 entre J e I, Vedado, CP 10400, La Habana, Cuba","institution_ids":["https://openalex.org/I2656126"]},{"raw_affiliation_string":"Centro de Estudio de Prote\u00ednas, Facultad de Biolog\u00eda, Universidad de la Habana, Calle 25 #455 entre J e I, Vedado, CP 10400, La Habana, Cuba","institution_ids":["https://openalex.org/I2656126"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5068306672","display_name":"Beatriz Garc\u00eda","orcid":"https://orcid.org/0000-0002-5235-5167","observed_orcids":["https://orcid.org/0000-0002-5235-5167"]},"institutions":[{"id":"https://openalex.org/I168635309","display_name":"University of Calgary","ror":"https://ror.org/03yjb2x39","country_code":"CA","type":"education","lineage":["https://openalex.org/I168635309"]},{"id":"https://openalex.org/I2656126","display_name":"University of Havana","ror":"https://ror.org/04204gr61","country_code":"CU","type":"education","lineage":["https://openalex.org/I2656126"]}],"countries":["CA","CU"],"is_corresponding":false,"raw_author_name":"Beatriz Garc\u00eda","raw_affiliation_strings":["3 Present address: Department of Biological Sciences, 2500 University Drive N.W., University of Calgary, Calgary, AB, Canada T2N 1N4","Centro de Estudio de Protei\u0301nas, Facultad de Biologi\u0301a, Universidad de la Habana, Calle 25 #455 entre J e I, Vedado, CP 10400, La Habana, Cuba","Centro de Estudio de Prote\u00ednas, Facultad de Biolog\u00eda, Universidad de la Habana, Calle 25 #455 entre J e I, Vedado, CP 10400, La Habana, Cuba"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"3 Present address: Department of Biological Sciences, 2500 University Drive N.W., University of Calgary, Calgary, AB, Canada T2N 1N4","institution_ids":["https://openalex.org/I168635309"]},{"raw_affiliation_string":"Centro de Estudio de Protei\u0301nas, Facultad de Biologi\u0301a, Universidad de la Habana, Calle 25 #455 entre J e I, Vedado, CP 10400, La Habana, Cuba","institution_ids":["https://openalex.org/I2656126"]},{"raw_affiliation_string":"Centro de Estudio de Prote\u00ednas, Facultad de Biolog\u00eda, Universidad de la Habana, Calle 25 #455 entre J e I, Vedado, CP 10400, La Habana, Cuba","institution_ids":["https://openalex.org/I2656126"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5000664349","display_name":"Colin Berry","orcid":"https://orcid.org/0000-0002-9943-548X","observed_orcids":["https://orcid.org/0000-0002-9943-548X"]},"institutions":[{"id":"https://openalex.org/I79510175","display_name":"Cardiff University","ror":"https://ror.org/03kk7td41","country_code":"GB","type":"education","lineage":["https://openalex.org/I79510175"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Colin Berry","raw_affiliation_strings":["Cardiff School of Biosciences, Cardiff University, Museum Avenue, Cardiff CF10 3US, Wales, U.K"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cardiff School of Biosciences, Cardiff University, Museum Avenue, Cardiff CF10 3US, Wales, U.K","institution_ids":["https://openalex.org/I79510175"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5043351826","display_name":"Judith Mendiola","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210149705","display_name":"Instituto de Medicina Tropical \u201cPedro Kour\u00ed\u201d","ror":"https://ror.org/05a9hae73","country_code":"CU","type":"facility","lineage":["https://openalex.org/I4210122314","https://openalex.org/I4210149705"]}],"countries":["CU"],"is_corresponding":false,"raw_author_name":"Judith Mendiola","raw_affiliation_strings":["Instituto de Medicina Tropical \u2018Pedro Kouri\u0301\u2019, Autopista Novia del Mediodi\u0301a, km 61\u20442 entre Carretera Central y Autopista Nacional, La Lisa, La Habana, Cuba","Instituto de Medicina Tropical 'Pedro Kour\u00ed', Autopista Novia del Mediod\u00eda, km 61\u20442 entre Carretera Central y Autopista Nacional, La Lisa, La Habana, Cuba"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Instituto de Medicina Tropical \u2018Pedro Kouri\u0301\u2019, Autopista Novia del Mediodi\u0301a, km 61\u20442 entre Carretera Central y Autopista Nacional, La Lisa, La Habana, Cuba","institution_ids":["https://openalex.org/I4210149705"]},{"raw_affiliation_string":"Instituto de Medicina Tropical 'Pedro Kour\u00ed', Autopista Novia del Mediod\u00eda, km 61\u20442 entre Carretera Central y Autopista Nacional, La Lisa, La Habana, Cuba","institution_ids":["https://openalex.org/I4210149705"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5035330456","display_name":"Aida Hern\u00e1ndez\u2010Zanui","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210123161","display_name":"Ministerio de Ciencia, Tecnolog\u00eda y Medio Ambiente","ror":"https://ror.org/02xqrgv78","country_code":"CU","type":"government","lineage":["https://openalex.org/I4210123161"]}],"countries":["CU"],"is_corresponding":false,"raw_author_name":"Aida Hern\u00e1ndez\u2010Zanui","raw_affiliation_strings":["Instituto de Oceanologi\u0301a, CITMA, Ave 1ra y 196, Playa, La Habana, Cuba","Instituto de Oceanolog\u00eda, CITMA, Ave 1ra y 196, Playa, La Habana, Cuba"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Instituto de Oceanologi\u0301a, CITMA, Ave 1ra y 196, Playa, La Habana, Cuba","institution_ids":["https://openalex.org/I4210123161"]},{"raw_affiliation_string":"Instituto de Oceanolog\u00eda, CITMA, Ave 1ra y 196, Playa, La Habana, Cuba","institution_ids":["https://openalex.org/I4210123161"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5109079663","display_name":"Mar\u00eda de los Angeles Ch\u00e1vez","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2656126","display_name":"University of Havana","ror":"https://ror.org/04204gr61","country_code":"CU","type":"education","lineage":["https://openalex.org/I2656126"]}],"countries":["CU"],"is_corresponding":true,"raw_author_name":"Mar\u00eda\u00a0de\u00a0los\u00a0A. Ch\u00e1vez","raw_affiliation_strings":["Centro de Estudio de Protei\u0301nas, Facultad de Biologi\u0301a, Universidad de la Habana, Calle 25 #455 entre J e I, Vedado, CP 10400, La Habana, Cuba","Centro de Estudio de Prote\u00ednas, Facultad de Biolog\u00eda, Universidad de la Habana, Calle 25 #455 entre J e I, Vedado, CP 10400, La Habana, Cuba"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centro de Estudio de Protei\u0301nas, Facultad de Biologi\u0301a, Universidad de la Habana, Calle 25 #455 entre J e I, Vedado, CP 10400, La Habana, Cuba","institution_ids":["https://openalex.org/I2656126"]},{"raw_affiliation_string":"Centro de Estudio de Prote\u00ednas, Facultad de Biolog\u00eda, Universidad de la Habana, Calle 25 #455 entre J e I, Vedado, CP 10400, La Habana, Cuba","institution_ids":["https://openalex.org/I2656126"]}]}],"institutions":[],"countries_distinct_count":3,"institutions_distinct_count":6,"corresponding_author_ids":["https://openalex.org/A5094072847","https://openalex.org/A5109079663"],"corresponding_institution_ids":["https://openalex.org/I2656126","https://openalex.org/I4210148836"],"apc_list":{"value":3470,"currency":"USD","value_usd":3470},"apc_paid":null,"fwci":1.5019,"has_fulltext":false,"cited_by_count":29,"citation_normalized_percentile":{"value":0.80126958,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":98},"biblio":{"volume":"52","issue":"2","first_page":"149","last_page":"157"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.33180001378059387,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.33180001378059387,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10091","display_name":"Malaria Research and Control","score":0.1808999925851822,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.17180000245571136,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/affinity-matrix","display_name":"affinity matrix","score":0.7689999938011169},{"id":"https://openalex.org/keywords/cerebrospinal-fluid","display_name":"cerebrospinal fluid","score":0.7940000295639038},{"id":"https://openalex.org/keywords/high-abundance-protein-depletion","display_name":"high-abundance protein depletion","score":0.8629999756813049},{"id":"https://openalex.org/keywords/affibody-molecules","display_name":"Affibody molecules","score":0.8209999799728394},{"id":"https://openalex.org/keywords/plasmepsin-ii","display_name":"plasmepsin II","score":0.8830000162124634},{"id":"https://openalex.org/keywords/antimalarial-drug-targets","display_name":"antimalarial drug targets","score":0.8999999761581421},{"id":"https://openalex.org/keywords/human-serum-albumin","display_name":"human serum albumin","score":0.8299999833106995},{"id":"https://openalex.org/keywords/igg","display_name":"IgG","score":0.8740000128746033},{"id":"https://openalex.org/keywords/transferrin","display_name":"transferrin","score":0.9919999837875366},{"id":"https://openalex.org/keywords/prealbumin","display_name":"prealbumin","score":1.0},{"id":"https://openalex.org/keywords/cystatin-c","display_name":"cystatin C","score":0.9599999785423279},{"id":"https://openalex.org/keywords/bottom-up-proteomics","display_name":"shotgun proteomics","score":0.7009999752044678}],"concepts":[{"id":"https://openalex.org/C2776350231","wikidata":"https://www.wikidata.org/wiki/Q7166509","display_name":"Pepstatin","level":4,"score":0.8923056125640869},{"id":"https://openalex.org/C2779881004","wikidata":"https://www.wikidata.org/wiki/Q7451876","display_name":"Sepharose","level":3,"score":0.5933610200881958},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5442741513252258},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.46897539496421814},{"id":"https://openalex.org/C179247698","wikidata":"https://www.wikidata.org/wiki/Q382799","display_name":"Affinity chromatography","level":3,"score":0.45219358801841736},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4427480399608612},{"id":"https://openalex.org/C116569031","wikidata":"https://www.wikidata.org/wiki/Q899107","display_name":"Ligand (biochemistry)","level":3,"score":0.4257878065109253},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3982670307159424},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3697490096092224},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.2141595184803009},{"id":"https://openalex.org/C2776714187","wikidata":"https://www.wikidata.org/wiki/Q212410","display_name":"Protease","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D000906","descriptor_name":"Antibodies","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002556","descriptor_name":"Cerebrospinal Fluid Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D002556","descriptor_name":"Cerebrospinal Fluid Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D002846","descriptor_name":"Chromatography, Affinity","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004797","descriptor_name":"Enzyme-Linked Immunosorbent Assay","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019032","descriptor_name":"Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D053000","descriptor_name":"Analytic Sample Preparation Methods","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1042/ba20080015","doi":"https://doi.org/10.1042/ba20080015","is_oa":false,"landing_page_url":"https://doi.org/10.1042/ba20080015","pdf_url":null,"source":{"id":"https://openalex.org/S34872747","display_name":"Biotechnology and Applied Biochemistry","issn_l":"0885-4513","issn":["0885-4513","1470-8744"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and Applied Biochemistry","raw_type":"journal-article"},{"id":"pmid:18412540","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/18412540","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and applied biochemistry","raw_type":"Journal Article"},{"id":"pmh:oai:research.chalmers.se:234371","doi":null,"is_oa":false,"landing_page_url":"https://research.chalmers.se/en/publication/234371","pdf_url":null,"source":{"id":"https://openalex.org/S4306402469","display_name":"Chalmers Research (Chalmers University of Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I66862912","host_organization_name":"Chalmers University of Technology","host_organization_lineage":["https://openalex.org/I66862912"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.9391000270843506}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":29,"referenced_works":["https://openalex.org/W36676292","https://openalex.org/W76995127","https://openalex.org/W104726253","https://openalex.org/W1581608952","https://openalex.org/W1945578658","https://openalex.org/W1987776161","https://openalex.org/W1992636402","https://openalex.org/W1993325600","https://openalex.org/W1997319983","https://openalex.org/W2004010329","https://openalex.org/W2013367982","https://openalex.org/W2017271523","https://openalex.org/W2019851585","https://openalex.org/W2024957471","https://openalex.org/W2033349111","https://openalex.org/W2055573265","https://openalex.org/W2063117415","https://openalex.org/W2078127009","https://openalex.org/W2079226740","https://openalex.org/W2087472718","https://openalex.org/W2100837269","https://openalex.org/W2106246537","https://openalex.org/W2111706745","https://openalex.org/W2114722238","https://openalex.org/W2116688431","https://openalex.org/W2128635872","https://openalex.org/W2164874913","https://openalex.org/W2165174924","https://openalex.org/W2214196579"],"related_works":["https://openalex.org/W2000280122","https://openalex.org/W2045404343","https://openalex.org/W2915252205","https://openalex.org/W2154575832","https://openalex.org/W7096661825","https://openalex.org/W2486000522","https://openalex.org/W2057141233","https://openalex.org/W40264796","https://openalex.org/W2087026681","https://openalex.org/W362899606"],"abstract_inverted_index":{"Various":[0],"approaches":[1],"for":[2,18,25,41,87,135,207,220,240],"removal":[3,184],"of":[4,30,44,71,102,138,185,194,258,269],"high-abundance":[5,45,130],"components":[6,260],"in":[7,47,62,94,98,111,114,117,126,158,176,225,243,263,267],"body":[8],"fluids":[9],"are":[10,16,68,83,105,108,123],"currently":[11],"available.":[12],"While":[13],"most":[14],"methods":[15],"constructed":[17],"plasma":[19],"depletion,":[20],"there":[21],"is":[22],"a":[23,89,139,177],"need":[24],"body-fluid-specific":[26],"strategies.":[27],"The":[28,91,192],"aim":[29],"the":[31,42,103,136,186,232,251,264],"present":[32,58,110],"study":[33],"was":[34,190,218,238,261],"to":[35,171,199],"design":[36,137],"an":[37,159],"affinity":[38,55,160],"matrix":[39],"suitable":[40],"depletion":[43,141],"proteins":[46,57,104,122,132,189,196,224],"CSF":[48,63,95,115,131,229,249],"(cerebrospinal":[49],"fluid).":[50],"Hence,":[51],"molecules":[52,67,144],"with":[53,145],"specific":[54,69],"towards":[56,147],"at":[59],"high":[60,77],"concentration":[61],"were":[64,133,156,169,197,254],"desired.":[65],"Affibody":[66,143],"binders":[70],"small":[72],"size":[73],"that":[74,97],"have":[75],"shown":[76],"stability":[78],"under":[79],"various":[80],"conditions":[81],"and":[82,107,154,174,182,205,212,222,231,250],"therefore":[84,109],"good":[85],"candidates":[86],"such":[88],"matrix.":[90],"protein":[92,241],"composition":[93],"resembles":[96],"plasma.":[99],"However,":[100],"20%":[101],"brain-derived":[106],"higher":[112],"proportions":[113],"than":[116],"plasma,":[118],"whereas":[119],"larger":[120],"plasma-derived":[121],"less":[124],"abundant":[125],"CSF.":[127],"Therefore":[128],"five":[129,187],"chosen":[134],"CSF-specific":[140],"setup.":[142],"specificity":[146],"HSA":[148],"(human":[149],"serum":[150],"albumin),":[151],"IgG,":[152,209],"transferrin":[153],"transthyretin":[155,211],"combined":[157],"column.":[161,179],"In":[162],"addition,":[163],"polyclonal":[164],"antibodies":[165],"against":[166],"cystatin":[167,213],"C":[168,214],"coupled":[170],"chromatographic":[172],"beads":[173],"packed":[175],"separate":[178],"Highly":[180],"reproducible":[181],"efficient":[183],"target":[188],"observed.":[191],"proportion":[193],"depleted":[195,228,248,265],"estimated":[198],"be":[200],"99,":[201],"95,":[202],"74,":[203],"92":[204],"83%":[206],"HSA,":[208],"transferrin,":[210],"respectively.":[215],"SDS/PAGE":[216],"analysis":[217],"used":[219,239],"monitoring":[221],"identifying":[223],"native":[226,244],"CSF,":[227],"samples":[230],"captured":[233],"fractions.":[234],"Moreover,":[235],"shotgun":[236],"proteomics":[237],"identification":[242,257],"as":[245,247],"well":[246],"achieved":[252],"data":[253],"compared.":[255],"Enhanced":[256],"lower-abundance":[259],"observed":[262],"fraction,":[266],"terms":[268],"more":[270],"detected":[271],"peptides":[272],"per":[273],"protein.":[274]},"counts_by_year":[{"year":2023,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2017,"cited_by_count":4},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":3},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":2}],"updated_date":"2026-11-12T12:28:57.059296","created_date":"2022-05-12T00:00:00"}