{"id":"https://openalex.org/W4240900430","doi":"https://doi.org/10.1055/s-0029-1214114","title":"T-RFLP \u2013 a representative tool to study pulmonary microbial communities","display_name":"T-RFLP \u2013 a representative tool to study pulmonary microbial communities","publication_year":2009,"publication_date":"2009-03-01","ids":{"openalex":"https://openalex.org/W4240900430","doi":"https://doi.org/10.1055/s-0029-1214114"},"language":"en","primary_location":{"id":"doi:10.1055/s-0029-1214114","doi":"https://doi.org/10.1055/s-0029-1214114","is_oa":false,"landing_page_url":"https://doi.org/10.1055/s-0029-1214114","pdf_url":null,"source":{"id":"https://openalex.org/S111957458","display_name":"Pneumologie","issn_l":"0934-8387","issn":["0934-8387","1438-8790"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310320000","host_organization_name":"Thieme Medical Publishers (Germany)","host_organization_lineage":["https://openalex.org/P4310320000"],"host_organization_lineage_names":["Thieme Medical Publishers (Germany)"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Pneumologie","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5111984272","display_name":"Tetyana Zakharkina","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I161103922","display_name":"Philipps University of Marburg","ror":"https://ror.org/01rdrb571","country_code":"DE","type":"education","lineage":["https://openalex.org/I161103922"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"T Zakharkina","raw_affiliation_strings":["Klinik f\u00fcr Innere Medizin mit SP Pneumologie, Philipps University of Marburg"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Klinik f\u00fcr Innere Medizin mit SP Pneumologie, Philipps University of Marburg","institution_ids":["https://openalex.org/I161103922"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5078234051","display_name":"C Herr","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I161103922","display_name":"Philipps University of Marburg","ror":"https://ror.org/01rdrb571","country_code":"DE","type":"education","lineage":["https://openalex.org/I161103922"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"C Herr","raw_affiliation_strings":["Klinik f\u00fcr Innere Medizin mit SP Pneumologie, Philipps University of Marburg"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Klinik f\u00fcr Innere Medizin mit SP Pneumologie, Philipps University of Marburg","institution_ids":["https://openalex.org/I161103922"]}]},{"author_position":"middle","author":{"id":null,"display_name":"A Yildirim","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I161103922","display_name":"Philipps University of Marburg","ror":"https://ror.org/01rdrb571","country_code":"DE","type":"education","lineage":["https://openalex.org/I161103922"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"A Yildirim","raw_affiliation_strings":["Klinik f\u00fcr Innere Medizin mit SP Pneumologie, Philipps University of Marburg"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Klinik f\u00fcr Innere Medizin mit SP Pneumologie, Philipps University of Marburg","institution_ids":["https://openalex.org/I161103922"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5041167874","display_name":"M Friedrich","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210166203","display_name":"Max Planck Institute for Terrestrial Microbiology","ror":"https://ror.org/05r7n9c40","country_code":"DE","type":"facility","lineage":["https://openalex.org/I149899117","https://openalex.org/I4210166203"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"M Friedrich","raw_affiliation_strings":["Max-Plank Institute for Terrestrial Microbiology, Marburg"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Max-Plank Institute for Terrestrial Microbiology, Marburg","institution_ids":["https://openalex.org/I4210166203"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5013694139","display_name":"R Bals","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I161103922","display_name":"Philipps University of Marburg","ror":"https://ror.org/01rdrb571","country_code":"DE","type":"education","lineage":["https://openalex.org/I161103922"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"R Bals","raw_affiliation_strings":["Klinik f\u00fcr Innere Medizin mit SP Pneumologie, Philipps University of Marburg"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Klinik f\u00fcr Innere Medizin mit SP Pneumologie, Philipps University of Marburg","institution_ids":["https://openalex.org/I161103922"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.58452167,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"63","issue":"S 01","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10143","display_name":"Chronic Obstructive Pulmonary Disease (COPD) Research","score":0.6147000193595886,"subfield":{"id":"https://openalex.org/subfields/2740","display_name":"Pulmonary and Respiratory Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10143","display_name":"Chronic Obstructive Pulmonary Disease (COPD) Research","score":0.6147000193595886,"subfield":{"id":"https://openalex.org/subfields/2740","display_name":"Pulmonary and Respiratory Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10257","display_name":"Oral microbiology and periodontitis research","score":0.10729999840259552,"subfield":{"id":"https://openalex.org/subfields/3506","display_name":"Periodontics"},"field":{"id":"https://openalex.org/fields/35","display_name":"Dentistry"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10066","display_name":"Gut microbiota and health","score":0.07989999651908875,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/pulmonary-microbiota","display_name":"pulmonary microbiota","score":0.8539999723434448},{"id":"https://openalex.org/keywords/lung-microbiome","display_name":"lung microbiome","score":0.6349999904632568},{"id":"https://openalex.org/keywords/chronic-obstructive-pulmonary-disease","display_name":"chronic obstructive pulmonary disease","score":0.8309999704360962},{"id":"https://openalex.org/keywords/bronchoalveolar-lavage","display_name":"bronchoalveolar lavage","score":0.9160000085830688},{"id":"https://openalex.org/keywords/16s-rrna-gene","display_name":"16S rRNA gene","score":0.8759999871253967},{"id":"https://openalex.org/keywords/t-rflp","display_name":"T-RFLP","score":0.9039999842643738},{"id":"https://openalex.org/keywords/bacterial-colonization","display_name":"bacterial colonization","score":0.8700000047683716},{"id":"https://openalex.org/keywords/haemophilus","display_name":"Haemophilus","score":0.871999979019165},{"id":"https://openalex.org/keywords/streptococcus","display_name":"Streptococcus","score":1.0},{"id":"https://openalex.org/keywords/lower-respiratory-tract","display_name":"lower respiratory tract","score":0.8519999980926514},{"id":"https://openalex.org/keywords/species-richness","display_name":"species richness","score":0.6570000052452087},{"id":"https://openalex.org/keywords/mouse-lung","display_name":"mouse lung","score":0.36500000953674316}],"concepts":[{"id":"https://openalex.org/C2779644171","wikidata":"https://www.wikidata.org/wiki/Q13400765","display_name":"Respiratory tract","level":3,"score":0.6523646712303162},{"id":"https://openalex.org/C2777714996","wikidata":"https://www.wikidata.org/wiki/Q7886","display_name":"Lung","level":2,"score":0.5464968085289001},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4712945222854614},{"id":"https://openalex.org/C2776780178","wikidata":"https://www.wikidata.org/wiki/Q199804","display_name":"COPD","level":2,"score":0.47124654054641724},{"id":"https://openalex.org/C534529494","wikidata":"https://www.wikidata.org/wiki/Q7891","display_name":"Respiratory system","level":2,"score":0.42999401688575745},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.3648661971092224},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.3520642817020416},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.1905195116996765},{"id":"https://openalex.org/C105702510","wikidata":"https://www.wikidata.org/wiki/Q514","display_name":"Anatomy","level":1,"score":0.09233611822128296}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1055/s-0029-1214114","doi":"https://doi.org/10.1055/s-0029-1214114","is_oa":false,"landing_page_url":"https://doi.org/10.1055/s-0029-1214114","pdf_url":null,"source":{"id":"https://openalex.org/S111957458","display_name":"Pneumologie","issn_l":"0934-8387","issn":["0934-8387","1438-8790"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310320000","host_organization_name":"Thieme Medical Publishers (Germany)","host_organization_lineage":["https://openalex.org/P4310320000"],"host_organization_lineage_names":["Thieme Medical Publishers (Germany)"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Pneumologie","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.9537000060081482}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W7096012342","https://openalex.org/W1966931970","https://openalex.org/W2327521301","https://openalex.org/W2134391000","https://openalex.org/W2011709488","https://openalex.org/W2155737151","https://openalex.org/W750467849","https://openalex.org/W2087626627","https://openalex.org/W2068332307","https://openalex.org/W2163401084"],"abstract_inverted_index":{"Background:":[0],"It":[1],"has":[2],"become":[3],"apparent":[4],"that":[5,60,84,235],"lungs":[6,40,213,264],"are":[7,61,238],"constantly":[8],"exposed":[9],"to":[10,34,65,89,110,170,200],"complex":[11],"microbial":[12,272],"communities.":[13],"Changes":[14],"in":[15,22,44,86,92,158,184,205,250],"such":[16],"communities":[17,273],"may":[18],"play":[19,201],"critical":[20],"role":[21,204],"the":[23,36,53,90,93,96,129,144,153,166,202,206,211,216,233,239,251,270,279,282],"initiation":[24],"of":[25,30,39,55,95,104,147,168,218,223,241,255,269,287],"lung":[26,124],"diseases.":[27],"The":[28,102,149,187,194],"aim":[29],"this":[31],"study":[32],"was":[33,108,126,161,191],"characterize":[35],"normal":[37],"microbiota":[38],"and":[41,79,122,141,197,221,258,262,274,285],"COPD-induced":[42],"alterations":[43],"lower":[45,236,252],"respiratory":[46,253],"tract.":[47],"Methods:":[48],"Conventional":[49],"culture-based":[50],"methods":[51],"for":[52,128,210],"analysis":[54],"pulmonary":[56,288],"flora":[57],"miss":[58],"bacteria":[59,182],"difficult":[62],"or":[63,176],"impossible":[64],"culture.":[66],"Therefore":[67],"we":[68],"applied":[69],"PCR-based":[70],"tools":[71],"\u2013":[72],"terminal":[73,133],"restriction":[74,99,134],"fragment":[75,103,135],"length":[76,87],"polymorphism":[77],"(T-RFLP)":[78],"sequencing.":[80],"T-RFLP":[81],"generates":[82],"fragments":[83],"differ":[85],"due":[88],"variation":[91],"position":[94,220],"first":[97],"specific":[98],"endonuclease":[100],"site.":[101],"16S":[105],"RNA":[106],"gene":[107],"chosen":[109],"identify":[111],"bacteria.":[112],"Total":[113],"DNA,":[114],"isolated":[115],"from":[116],"clinical":[117],"bronchoalveolar":[118],"lavage":[119],"(BAL)":[120],"samples":[121],"mice":[123,212,263],"sections":[125],"used":[127],"analysis.":[130],"Results:":[131],"Generated":[132],"(T-RF)":[136],"patterns":[137],"revealed":[138],"both":[139],"similarities":[140],"differences":[142],"inside":[143],"same":[145],"group":[146],"patients.":[148],"frequency":[150],"at":[151],"which":[152],"T-RF":[154],"bands":[155],"were":[156,228],"detected":[157,247],"each":[159],"sample":[160],"determined.":[162],"Universal":[163],"peaks":[164,179,224],"demonstrated":[165],"presence":[167],"1":[169],"3":[171],"major":[172],"bacterial":[173,243],"pathogens.":[174],"Unique":[175],"rarely":[177],"observed":[178],"reflect":[180],"individual":[181],"present":[183],"different":[185,226],"samples.":[186],"mean":[188],"species":[189,249],"richness":[190],"13.2":[192],"(\u00b14.4).":[193],"genera":[195],"Haemophillus":[196],"Streptococcus":[198],"seem":[199],"leading":[203],"colonization":[207],"process.":[208],"As":[209],"tissue":[214],"samples,":[215],"reproducibility":[217],"number,":[219],"intensity":[222],"by":[225],"animals":[227],"observed.":[229],"This":[230],"data":[231],"support":[232],"idea,":[234],"airways":[237],"site":[240],"stable":[242],"colonization.":[244],"Discussion:":[245],"We":[246],"various":[248],"tract":[254],"healthy":[256],"persons":[257],"patients":[259],"with":[260],"COPD":[261],"sections.":[265],"A":[266],"better":[267],"understanding":[268],"resident":[271],"their":[275],"dynamics":[276],"should":[277],"shed":[278],"light":[280],"on":[281],"pathogenesis,":[283],"diagnosis":[284],"treatment":[286],"disorders.":[289]},"counts_by_year":[],"updated_date":"2026-09-29T14:09:16.559479","created_date":"2022-05-12T00:00:00"}