{"id":"https://openalex.org/W4239869684","doi":"https://doi.org/10.1201/b18096-9","title":"Oral Microparticulate Vaccine for Melanoma","display_name":"Oral Microparticulate Vaccine for Melanoma","publication_year":2015,"publication_date":"2015-05-28","ids":{"openalex":"https://openalex.org/W4239869684","doi":"https://doi.org/10.1201/b18096-9"},"language":"en","primary_location":{"id":"doi:10.1201/b18096-9","doi":"https://doi.org/10.1201/b18096-9","is_oa":false,"landing_page_url":"https://doi.org/10.1201/b18096-9","pdf_url":null,"source":{"id":"https://openalex.org/S4306463721","display_name":"Pan Stanford eBooks","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"ebook platform"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nanoparticulate Vaccine Delivery Systems","raw_type":"book-chapter"},"volume":{"id":"https://openalex.org/W2505892149","display_name":"Nanoparticulate Vaccine Delivery Systems"},"type":"book-chapter","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5061578762","display_name":"Bernadette D\u2019Souza","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Bernadette D'Souza","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5071397624","display_name":"Gary W. Bumgarner","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Gary Bumgarner","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5018895171","display_name":"Sucheta D\u2019Sa","orcid":"https://orcid.org/0000-0003-1055-1650","observed_orcids":["https://orcid.org/0000-0003-1055-1650"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Sucheta D'Sa","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5003736669","display_name":"Tuhin Bhowmik","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Tuhin Bhowmik","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101901410","display_name":"Periasamy Selvaraj","orcid":"https://orcid.org/0000-0003-3130-0551","observed_orcids":["https://orcid.org/0000-0003-3130-0551"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Periasamy Selvaraj","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5104010025","display_name":"Martin J. D\u2019Souza","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Martin D'Souza","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.58303249,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":"157","last_page":"170"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10873","display_name":"Bacterial Infections and Vaccines","score":0.9330999851226807,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10873","display_name":"Bacterial Infections and Vaccines","score":0.9330999851226807,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10580","display_name":"Immunotherapy and Immune Responses","score":0.03629999980330467,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10920","display_name":"Advanced Drug Delivery Systems","score":0.007699999958276749,"subfield":{"id":"https://openalex.org/subfields/3003","display_name":"Pharmaceutical Science"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/neisseria-meningitidis","display_name":"Neisseria meningitidis","score":0.8809999823570251},{"id":"https://openalex.org/keywords/meningococcal-vaccine","display_name":"meningococcal vaccine","score":0.7649999856948853},{"id":"https://openalex.org/keywords/oral-vaccine","display_name":"oral vaccine","score":0.5389999747276306},{"id":"https://openalex.org/keywords/capsular-polysaccharide","display_name":"capsular polysaccharide","score":0.8230000138282776},{"id":"https://openalex.org/keywords/serogroup-a","display_name":"serogroup A","score":0.8759999871253967},{"id":"https://openalex.org/keywords/conjugate-vaccines","display_name":"conjugate vaccines","score":0.6970000267028809}],"concepts":[{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.4919743239879608},{"id":"https://openalex.org/C16005928","wikidata":"https://www.wikidata.org/wiki/Q171171","display_name":"Dermatology","level":1,"score":0.44035714864730835},{"id":"https://openalex.org/C2777658100","wikidata":"https://www.wikidata.org/wiki/Q180614","display_name":"Melanoma","level":2,"score":0.4330514669418335},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.10193079710006714}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1201/b18096-9","doi":"https://doi.org/10.1201/b18096-9","is_oa":false,"landing_page_url":"https://doi.org/10.1201/b18096-9","pdf_url":null,"source":{"id":"https://openalex.org/S4306463721","display_name":"Pan Stanford eBooks","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"ebook platform"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nanoparticulate Vaccine Delivery Systems","raw_type":"book-chapter"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":1.0,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":22,"referenced_works":["https://openalex.org/W50975393","https://openalex.org/W1661544713","https://openalex.org/W1828940258","https://openalex.org/W1950572133","https://openalex.org/W1973089244","https://openalex.org/W1997215217","https://openalex.org/W1997408488","https://openalex.org/W2003444408","https://openalex.org/W2007518207","https://openalex.org/W2008131582","https://openalex.org/W2015058083","https://openalex.org/W2032753327","https://openalex.org/W2059557084","https://openalex.org/W2065984439","https://openalex.org/W2081774451","https://openalex.org/W2089716915","https://openalex.org/W2099289479","https://openalex.org/W2111986178","https://openalex.org/W2118277431","https://openalex.org/W2147822966","https://openalex.org/W2151592384","https://openalex.org/W2155461614"],"related_works":["https://openalex.org/W2337325108","https://openalex.org/W7100525397","https://openalex.org/W2032364697","https://openalex.org/W4200622204","https://openalex.org/W2117364171","https://openalex.org/W2167142248","https://openalex.org/W2418886007","https://openalex.org/W2124503244","https://openalex.org/W2124268111","https://openalex.org/W7025312114"],"abstract_inverted_index":{"Neisseria":[0],"meningitidis":[1,159],"is":[2,12,57,89,99,160,180,280,326,356,458],"a":[3,14,39,64,92,161,183,195,367,374,415,427,500,547,560,624,636,707,767,772,998],"major":[4,15,40],"cause":[5,163],"of":[6,91,94,105,127,132,139,164,249,277,289,298,320,361,396,412,476,578,701,703,710,775,785,804,807,867,890,909,953,958,973,989,1012,1030],"meningitis":[7,166],"and":[8,11,46,53,68,118,149,167,172,179,216,241,258,303,306,318,322,345,348,425,446,482,520,526,534,615,676,694,718,736,752,830,839,861,876,899,931,970,1017],"fulminant":[9],"septicemia":[10],"also":[13,28,78],"reason":[16],"for":[17,50,212,381,629,670,720,883,928,1002,1025],"mortality":[18,185],"associated":[19,181],"with":[20,182,465,487,514,522,538,582,723,729,982],"epidemics":[21],"that":[22,98,342,371,384,406,598],"occur":[23],"periodically":[24],"in":[25,43,59,169,175,214,265,282,388,401,529,696,713,749,810,848,893,997],"sub-Saharan":[26],"Africa,":[27],"known":[29],"as":[30,308,310,373,496,498,555,593,744,946],"the":[31,48,60,72,102,106,176,275,287,291,296,315,379,408,463,477,488,493,556,566,575,585,599,604,732,742,746,756,780,805,808,811,820,823,831,849,857,877,900,932,937,942,1018,1028,1031],"\u201cmeningitis":[32],"belt\u201d":[33],"[1].":[34,949],"Capsular":[35],"polysaccharides":[36],"(CPS)":[37],"are":[38,210,219,223,262,385,437,544,802],"virulence":[41],"factor":[42],"meningococcal":[44,108,208,228,237,246,253,278,299,339,369,397,579,587,630,704,790],"infections":[45],"form":[47],"basis":[49],"serogroup":[51,86,254],"designation":[52],"protective":[54],"vaccines.":[55,332,390],"CPS":[56,88,398,413,794],"anchored":[58],"outer":[61],"membrane":[62],"through":[63,766],"1,2-diacylglycerol":[65],"moiety":[66],"[2]":[67],"functions":[69],"to":[70,193,268,328,336,351,365,414,434,450,491,531,612,622,741,778,827,872,935,965,979,986,1007],"protect":[71],"bacteria":[73],"from":[74,101,639,650,661,673,681,690],"complement-mediated":[75],"killing":[76],"while":[77],"inhibiting":[79],"phagocytosis":[80],"by":[81,199,461,518,546,559,617,853,915,941],"professional":[82],"phagocytes":[83],"[1,3].":[84],"The":[85,204,359,391,505,542,762,799,920],"A":[87,590],"composed":[90],"homopolymer":[93],"a1\uf0ae6-linked":[95],"ManNAc":[96],"1-phosphate":[97],"distinct":[100],"capsular":[103],"structures":[104],"other":[107,515],"Rikhav":[109],"P.":[110],"Gala,a":[111],"Ruhi":[112],"V.":[113],"Ubale,b":[114],"Martin":[115],"J.":[116],"D\u2019Souza,a":[117],"Susu":[119],"M.":[120],"ZughaiercaMercer":[121],"University,":[122],"Atlanta,":[123,141],"GA,":[124,142],"USAbLECOM":[125],"School":[126,138],"Pharmacy,":[128],"Bradenton,":[129],"FL,":[130],"USAcDepartment":[131],"Microbiology":[133],"&":[134],"Immunology,":[135],"Emory":[136],"University":[137],"Medicine,":[140],"USA":[143],"disease-causing":[144],"serogroups,":[145],"B,":[146],"C,":[147,256],"Y,":[148],"W-135,":[150],"which":[151,418,819,906,993],"typically":[152],"contain":[153,252],"sialic":[154],"acid":[155,944],"derivatives":[156],"[4].":[157],"N.":[158],"leading":[162],"bacterial":[165],"sepsis":[168],"young":[170,173,284,309],"children":[171,285],"adults":[174,217],"United":[177],"States":[178],"high":[184,316],"rate":[186,774],"[5,6].":[187],"Childhood":[188],"vaccination":[189],"has":[190,509],"been":[191,511],"shown":[192],"induce":[194,444,499],"herd":[196],"immunity":[197,347],"effect":[198],"reducing":[200],"nasopharyngeal":[201],"carriage":[202],"[4,7].":[203],"current":[205],"available":[206,479],"polysaccharide-based":[207,516],"vaccines":[209,222,251,261,301,325,480,517,536],"licensed":[211,331],"use":[213,297],"adolescents":[215],"but":[218,602],"expensive.":[220],"These":[221],"Menactra\u2122":[224],"(Sanofi":[225,233],"Pasteur":[226,234],"quadrivalent":[227,236,244],"conjugate":[229,245,300,324],"MCV4":[230],"vaccine),":[231,240],"Menomune\u00ae":[232],"unconjugated":[235],"polysaccharide":[238,435,464,705,939,991],"MPSV4":[239],"Menveo\u00ae":[242,483],"(Novartis":[243],"vaccine).":[247],"All":[248],"these":[250],"A,":[255],"W135,":[257],"Y.":[259],"Meningococcal":[260],"poorly":[263],"immunogenic":[264,467],"infants":[266,307],"due":[267],"their":[269,613],"under-developed":[270],"immune":[271,429,453,494,502,609],"responses":[272,433,454,610],"[8].":[273],"Since":[274],"incidence":[276],"disease":[279],"highest":[281],"very":[283,293,357],"under":[286],"age":[288],"two,":[290],"FDA":[292],"recently":[294],"approved":[295],"Menatcra\u2122":[302,481],"Menveo\u00aein":[304],"toddles":[305],"9":[311],"months":[312],"old.":[313],"However,":[314],"cost":[317],"limitation":[319],"producing":[321],"distributing":[323],"inherent":[327],"all":[329],"currently":[330,386],"Therefore,":[333],"utilizing":[334],"micro/nanotechnology":[335],"explore":[337],"novel":[338,576,625],"vaccine":[340,370,393,507],"formulations":[341],"boost":[343],"innate":[344,608],"adaptive":[346],"offer":[349],"protection":[350],"our":[352],"most":[353,389],"vulnerable":[354],"population":[355],"important.":[358],"aim":[360],"this":[362,697],"study":[363],"was":[364,635,648,659,716,727,764,814,825,833,851,870,879,897,902,912,922,984,994,1015,1020,1034],"formulate":[366],"microparticulate":[368],"serves":[372],"sustained":[375],"release":[376],"system,":[377],"obviating":[378],"need":[380],"added":[382,740,826,871,898,913,964,985,1016],"adjuvants":[383],"present":[387],"proposed":[392],"formulation":[394,508,577],"consists":[395],"polymers":[399],"encapsulated":[400],"albumin-based":[402],"biodegradable":[403],"matrix":[404,558],"microparticles":[405,543,581,601,788,850,875],"mimic":[407],"chemical":[409,471,485],"conjugation":[410,472,486],"process":[411,549,564],"protein":[416,469,557,656],"carrier,":[417],"enhance":[419],"antigen":[420,821],"uptake":[421,614],"via":[422],"albumin":[423,553,786],"receptors":[424],"elicit":[426],"T-cell-dependent":[428,501],"response":[430,495,503],"[9-11].":[431],"Immune":[432],"antigens":[436,735],"inherently":[438],"thymus":[439,451],"independent,":[440],"therefore":[441],"do":[442],"not":[443,603],"memory":[445],"hence":[447],"require":[448],"conversion":[449,457],"dependent":[452],"[12].":[455],"Such":[456],"usually":[459],"achieved":[460],"coupling":[462],"an":[466],"carrier":[468],"using":[470,550,565,755,816,859],"processes":[473],"[13,12].":[474],"Two":[475],"commercially":[478],"involve":[484],"Diphtheria":[489],"toxoid":[490],"potentiate":[492],"well":[497],"[13].":[504],"conjugation-free":[506],"previously":[510],"successfully":[512],"tested":[513],"D\u2019Souza":[519],"co-workers":[521],"typhoid":[523],"[6],":[524],"TB,":[525],"Streptococcus":[527],"pneumonia,":[528],"addition":[530],"melanoma":[532],"[11],":[533],"influenza":[535],"designed":[537],"similar":[539],"polymer":[540],"matrices.":[541],"prepared":[545,717],"well-established":[548],"bovine":[551,844],"serum":[552,845],"(BSA)":[554],"one-step":[561],"spray":[562,569,753,835],"drying":[563],"Buchi":[567,757],"mini":[568],"dryer":[570],"B-191":[571],"[11].We":[572],"have":[573,620],"report":[574],"CPS-loaded":[580,600],"or":[583,737,793],"without":[584],"non-toxic":[586],"unglycosylated":[588],"lipid":[589],"(kdtA)":[591],"[14]":[592],"adjuvant.":[594],"Here":[595],"we":[596],"show":[597],"empty":[605],"microparticles,":[606,706],"induced":[607],"consequent":[611],"recognition":[616],"macrophages.":[618],"We":[619],"attempted":[621],"describe":[623],"microparticle-based":[626],"delivery":[627],"system":[628],"vaccine.":[631],"6.2":[632],"MethodsEudragit":[633],"S":[634],"generous":[637],"gift":[638],"Evonik":[640],"Degussa":[641],"Corporation":[642],"Parsippany,":[643],"NJ":[644],"07054,":[645],"USA.":[646],"Trehalose":[647],"purchased":[649,660,689],"Sigma":[651],"Aldrich.":[652],"Bicinchoninic":[653],"Acid":[654],"(BCA)":[655],"assay":[657,672,945],"kit":[658],"Thermo":[662],"Scientific,":[663],"chicken":[664],"red":[665],"blood":[666],"cells":[667,669],"(RBC)":[668],"hemagglutination":[671],"Lampire":[674],"Biologicals,":[675],"ELISA":[677],"flat":[678],"bottom":[679],"plates":[680],"NUNC.":[682],"BALB/c":[683],"mice":[684],"(6-8":[685],"weeks":[686],"old)":[687],"were":[688,739],"Harlan":[691],"(Indianapolis,":[692],"IN)":[693],"used":[695],"study.":[698],"6.2.1":[699],"Preparation":[700,784],"MicroparticlesPreparation":[702],"1%":[708,891],"solution":[709,743,763,963],"sterile":[711,714,838],"BSA":[712,813],"water":[715,829,1000],"kept":[719],"overnight":[721,917],"cross-linking":[722],"glutaraldehyde.":[724],"Excess":[725],"glutaraldehyde":[726,817],"neutralized":[728],"sodium":[730],"bisulphate":[731],"following":[733,818],"day.Polysaccharide":[734],"adjuvant":[738,796,824],"per":[745],"concentrations":[747,800],"mentioned":[748],"Table":[750,782],"6.1":[751,783],"dried":[754],"Mini":[758],"Spray":[759],"Dryer":[760],"B-191.":[761],"passed":[765],"0.5":[768],"mm":[769],"nozzle":[770],"at":[771,881,918,925,1022,1036],"flow":[773],"20":[776],"mL/h":[777],"obtain":[779],"microparticles.":[781],"(BSA)-based":[787],"containing":[789],"CPS,":[791],"adjuvant,":[792],"+":[795],"Formulation":[797],"Note:":[798],"listed":[801],"representative":[803],"percentage":[806],"constituent":[809],"formulation.":[812],"cross-linked":[815],"and/or":[822],"deionized":[828],"mixture":[832,878,901,921,1019],"then":[834,923,995],"dried.":[836],"BSA:":[837],"endotoxin-free":[840],"tissue":[841],"culture":[842],"grade":[843],"albumin.Polysaccharide":[846],"content":[847,940],"measured":[852],"initially":[854],"breaking":[855],"down":[856],"particle":[858],"surfactants":[860],"protease":[862],"enzymes.":[863],"Briefly,":[864,950],"200":[865,888],"\u00b5L":[866,889],"Tris-EDTA":[868],"buffer":[869],"5":[873,1010],"mg":[874],"incubated":[880],"37\u00b0C":[882],"4":[884],"h.":[885],"Following":[886],"this,":[887],"SDS":[892],"0.2":[894],"N":[895],"NaOH":[896],"vortexed":[903],"repeatedly,":[904],"after":[905],"50":[907],"\u00b5g":[908],"proteinase":[910],"K":[911],"followed":[914],"incubation":[916],"37\u00b0C.":[919],"centrifuged":[924,1021],"14,000":[926],"rpm":[927,1024],"15":[929,1003],"min":[930],"supernatant":[933],"analyzed":[934],"determine":[936],"extracted":[938,990],"resorcinol-hydrochloric":[943],"described":[947],"earlier":[948],"1":[951,987,1026],"mL":[952,957,967,972,981,988,1011],"resorcinol-HCl":[954],"reagent":[955],"(10":[956],"2%":[959],"w/v":[960],"aq.":[961],"resorcinol":[962],"30":[966],"concentrated":[968],"HCl":[969],"0.25":[971],"0.1":[974],"M":[975],"CuSO4,":[976],"volume":[977],"adjusted":[978],"100":[980],"water)":[983],"sample,":[992],"heated":[996],"boiling":[999],"bath":[1001],"min.":[1004],"After":[1005],"cooling":[1006],"room":[1008],"temperature,":[1009],"amyl":[1013],"alcohol":[1014],"1000":[1023],"min;":[1027],"absorbance":[1029],"upper":[1032],"phase":[1033],"read":[1035],"580":[1037],"nm.":[1038]},"counts_by_year":[],"updated_date":"2026-10-10T09:11:00.788812","created_date":"2022-05-12T00:00:00"}