{"id":"https://openalex.org/W4239545256","doi":"https://doi.org/10.17504/protocols.io.gssbwee","title":"Epifluorescent Microscopy of Virus Particles Using SYBR Green v1","display_name":"Epifluorescent Microscopy of Virus Particles Using SYBR Green v1","publication_year":2016,"publication_date":"2016-12-16","ids":{"openalex":"https://openalex.org/W4239545256","doi":"https://doi.org/10.17504/protocols.io.gssbwee"},"language":"en","primary_location":{"id":"doi:10.17504/protocols.io.gssbwee","doi":"https://doi.org/10.17504/protocols.io.gssbwee","is_oa":true,"landing_page_url":"https://doi.org/10.17504/protocols.io.gssbwee","pdf_url":null,"source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://doi.org/10.17504/protocols.io.gssbwee","any_repository_has_fulltext":null},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5012545388","display_name":"Steven W. Wilhelm","orcid":"https://orcid.org/0000-0001-6283-8077","observed_orcids":["https://orcid.org/0000-0001-6283-8077"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Steven W Wilhelm","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5012545388"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11048","display_name":"Bacteriophages and microbial interactions","score":0.9595000147819519,"subfield":{"id":"https://openalex.org/subfields/2406","display_name":"Virology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11048","display_name":"Bacteriophages and microbial interactions","score":0.9595000147819519,"subfield":{"id":"https://openalex.org/subfields/2406","display_name":"Virology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11791","display_name":"Microbial Community Ecology and Physiology","score":0.010599999688565731,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10506","display_name":"Aquaculture disease management and microbiota","score":0.00279999990016222,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/virus-abundance","display_name":"viral abundance","score":0.6830000281333923},{"id":"https://openalex.org/keywords/virus-particles","display_name":"virus particles","score":0.800000011920929},{"id":"https://openalex.org/keywords/epifluorescence-microscopy","display_name":"epifluorescence microscopy","score":0.9480000138282776},{"id":"https://openalex.org/keywords/fluorescent-staining","display_name":"fluorescent staining","score":0.5180000066757202},{"id":"https://openalex.org/keywords/virus-detection","display_name":"virus detection","score":0.30399999022483826}],"concepts":[{"id":"https://openalex.org/C147080431","wikidata":"https://www.wikidata.org/wiki/Q1074953","display_name":"Microscopy","level":2,"score":0.6074594259262085},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.3790663480758667},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.36207354068756104},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.3286043405532837},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.31911250948905945},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.29752957820892334},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.23807483911514282},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.23162105679512024}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.17504/protocols.io.gssbwee","doi":"https://doi.org/10.17504/protocols.io.gssbwee","is_oa":true,"landing_page_url":"https://doi.org/10.17504/protocols.io.gssbwee","pdf_url":null,"source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.17504/protocols.io.gssbwee","doi":"https://doi.org/10.17504/protocols.io.gssbwee","is_oa":true,"landing_page_url":"https://doi.org/10.17504/protocols.io.gssbwee","pdf_url":null,"source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4234984314","https://openalex.org/W4239561497","https://openalex.org/W3154336566","https://openalex.org/W2905082591","https://openalex.org/W91472896","https://openalex.org/W3134435873","https://openalex.org/W2102463301","https://openalex.org/W1974021674","https://openalex.org/W4235606669","https://openalex.org/W4232005845"],"abstract_inverted_index":{"Adapted":[0],"from:":[1],"Ortmann":[2],"and":[3],"Suttle":[4],"(2009)":[5],"Determination":[6],"of":[7,16],"Virus":[8],"Abundance":[9],"by":[10],"Epifluorescence":[11],"microscopy.":[12],"Ch.":[13],"10":[14],"Methods":[15],"Molecular":[17],"Biology.":[18]},"counts_by_year":[],"updated_date":"2026-10-28T10:33:24.094632","created_date":"2022-05-12T00:00:00"}