{"id":"https://openalex.org/W4239198212","doi":"https://doi.org/10.3389/conf.fgene.2015.01.00069","title":"Genotoxicity assessment of antidiabetic formulation (ADPHF6) in human lymphocytes by single cell gel electrophoresis (comet assay) - an in vitro study","display_name":"Genotoxicity assessment of antidiabetic formulation (ADPHF6) in human lymphocytes by single cell gel electrophoresis (comet assay) - an in vitro study","publication_year":2015,"publication_date":"2015-01-01","ids":{"openalex":"https://openalex.org/W4239198212","doi":"https://doi.org/10.3389/conf.fgene.2015.01.00069"},"language":"en","primary_location":{"id":"doi:10.3389/conf.fgene.2015.01.00069","doi":"https://doi.org/10.3389/conf.fgene.2015.01.00069","is_oa":true,"landing_page_url":"https://doi.org/10.3389/conf.fgene.2015.01.00069","pdf_url":null,"source":{"id":"https://openalex.org/S2597340651","display_name":"Frontiers in Genetics","issn_l":"1664-8021","issn":["1664-8021"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","jufo-1","ki-jl-1","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Genetics","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.3389/conf.fgene.2015.01.00069","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5088939854","display_name":"Devanand Shanmugasundaram","orcid":"https://orcid.org/0000-0003-2650-6395","observed_orcids":["https://orcid.org/0000-0003-2650-6395","https://orcid.org/0000-0002-6763-8223"]},"institutions":[{"id":"https://openalex.org/I215927","display_name":"University of Madras","ror":"https://ror.org/04jmt9361","country_code":"IN","type":"education","lineage":["https://openalex.org/I215927"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Shanmugasundaram Devanand","raw_affiliation_strings":["Frontier Mediville (Affiliated to University of Madras)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Frontier Mediville (Affiliated to University of Madras)","institution_ids":["https://openalex.org/I215927"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5061712230","display_name":"Kumar Perumal","orcid":"https://orcid.org/0000-0001-5291-4224","observed_orcids":["https://orcid.org/0000-0001-5291-4224"]},"institutions":[{"id":"https://openalex.org/I20107917","display_name":"Sri Ramachandra Institute of Higher Education and Research","ror":"https://ror.org/0108gdg43","country_code":"IN","type":"education","lineage":["https://openalex.org/I20107917"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Perumal Kumar","raw_affiliation_strings":["Sri Ramachandra University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Sri Ramachandra University","institution_ids":["https://openalex.org/I20107917"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Sasikumar Changam","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210104337","display_name":"Frontier Lifeline Hospital","ror":"https://ror.org/00y5kz467","country_code":"IN","type":"healthcare","lineage":["https://openalex.org/I4210104337"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Sasikumar Changam","raw_affiliation_strings":["Frontier Lifeline Hospital"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Frontier Lifeline Hospital","institution_ids":["https://openalex.org/I4210104337"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Cherian Sanjay","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210104337","display_name":"Frontier Lifeline Hospital","ror":"https://ror.org/00y5kz467","country_code":"IN","type":"healthcare","lineage":["https://openalex.org/I4210104337"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Cherian Sanjay","raw_affiliation_strings":["Frontier Lifeline Hospital"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Frontier Lifeline Hospital","institution_ids":["https://openalex.org/I4210104337"]}]},{"author_position":"last","author":{"id":null,"display_name":"Cherian Kotturathu","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Cherian Kotturathu","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3150,"currency":"CHF","value_usd":3801},"apc_paid":{"value":3150,"currency":"CHF","value_usd":3801},"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"6","issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10433","display_name":"Carcinogens and Genotoxicity Assessment","score":0.9970999956130981,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10433","display_name":"Carcinogens and Genotoxicity Assessment","score":0.9970999956130981,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11140","display_name":"Natural Antidiabetic Agents Studies","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/2712","display_name":"Endocrinology, Diabetes and Metabolism"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/genotoxicity","display_name":"genotoxicity","score":0.9440000057220459},{"id":"https://openalex.org/keywords/polyherbal-formulation","display_name":"polyherbal formulation","score":0.765999972820282},{"id":"https://openalex.org/keywords/antidiabetic-herbal-formulation","display_name":"antidiabetic herbal formulation","score":0.6759999990463257},{"id":"https://openalex.org/keywords/oxidative-dna-damage","display_name":"oxidative DNA damage","score":0.7799999713897705},{"id":"https://openalex.org/keywords/hydrogen-peroxide-induced-dna-damage","display_name":"hydrogen peroxide-induced DNA damage","score":0.8360000252723694},{"id":"https://openalex.org/keywords/human-lymphocytes","display_name":"human lymphocytes","score":0.8889999985694885},{"id":"https://openalex.org/keywords/reactive-oxygen-species","display_name":"reactive oxygen species","score":0.8389999866485596},{"id":"https://openalex.org/keywords/oxidative-stress","display_name":"oxidative stress","score":0.8970000147819519},{"id":"https://openalex.org/keywords/antioxidant-defense","display_name":"antioxidant defense","score":0.6639999747276306},{"id":"https://openalex.org/keywords/hyperglycemia","display_name":"hyperglycemia","score":0.656000018119812},{"id":"https://openalex.org/keywords/alpha-glucosidase-inhibition","display_name":"\u03b1-glucosidase inhibition","score":0.7170000076293945},{"id":"https://openalex.org/keywords/alpha-amylase-inhibition","display_name":"\u03b1-amylase inhibition","score":0.9860000014305115},{"id":"https://openalex.org/keywords/free-radical-scavenging-activity","display_name":"free radical scavenging","score":0.847000002861023},{"id":"https://openalex.org/keywords/herbal-medicine-safety","display_name":"herbal medicine safety","score":0.4259999990463257},{"id":"https://openalex.org/keywords/alkaline-comet-assay","display_name":"alkaline comet assay","score":0.46000000834465027}],"concepts":[{"id":"https://openalex.org/C2780358455","wikidata":"https://www.wikidata.org/wiki/Q903780","display_name":"Comet assay","level":4,"score":0.8499586582183838},{"id":"https://openalex.org/C2778959862","wikidata":"https://www.wikidata.org/wiki/Q1009245","display_name":"Genotoxicity","level":3,"score":0.717056930065155},{"id":"https://openalex.org/C143425029","wikidata":"https://www.wikidata.org/wiki/Q5205747","display_name":"DNA damage","level":3,"score":0.6504158973693848},{"id":"https://openalex.org/C53227056","wikidata":"https://www.wikidata.org/wiki/Q189152","display_name":"Viability assay","level":3,"score":0.5779023170471191},{"id":"https://openalex.org/C2776151105","wikidata":"https://www.wikidata.org/wiki/Q898814","display_name":"Oxidative stress","level":2,"score":0.5100205540657043},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4725983738899231},{"id":"https://openalex.org/C116084860","wikidata":"https://www.wikidata.org/wiki/Q48255","display_name":"Gel electrophoresis","level":2,"score":0.47244957089424133},{"id":"https://openalex.org/C2780070996","wikidata":"https://www.wikidata.org/wiki/Q61482373","display_name":"Trypan blue","level":3,"score":0.4698046147823334},{"id":"https://openalex.org/C48349386","wikidata":"https://www.wikidata.org/wiki/Q424361","display_name":"Reactive oxygen species","level":2,"score":0.4542158842086792},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4214920699596405},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.3673423230648041},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3606957495212555},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3128790855407715},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.14639383554458618},{"id":"https://openalex.org/C29730261","wikidata":"https://www.wikidata.org/wiki/Q274160","display_name":"Toxicity","level":2,"score":0.11134758591651917},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.3389/conf.fgene.2015.01.00069","doi":"https://doi.org/10.3389/conf.fgene.2015.01.00069","is_oa":true,"landing_page_url":"https://doi.org/10.3389/conf.fgene.2015.01.00069","pdf_url":null,"source":{"id":"https://openalex.org/S2597340651","display_name":"Frontiers in Genetics","issn_l":"1664-8021","issn":["1664-8021"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","jufo-1","ki-jl-1","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Genetics","raw_type":"journal-article"},{"id":"pmh:oai:doaj.org/article:371e307eea2e4034b2b74968cf9f516f","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/371e307eea2e4034b2b74968cf9f516f","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Frontiers in Genetics, Vol 6 (2015)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.3389/conf.fgene.2015.01.00069","doi":"https://doi.org/10.3389/conf.fgene.2015.01.00069","is_oa":true,"landing_page_url":"https://doi.org/10.3389/conf.fgene.2015.01.00069","pdf_url":null,"source":{"id":"https://openalex.org/S2597340651","display_name":"Frontiers in Genetics","issn_l":"1664-8021","issn":["1664-8021"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","jufo-1","ki-jl-1","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Genetics","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.9763000011444092}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2369869274","https://openalex.org/W2763824032","https://openalex.org/W1975434946","https://openalex.org/W2008890172","https://openalex.org/W7055990998","https://openalex.org/W2975894187","https://openalex.org/W773185006","https://openalex.org/W7101159602","https://openalex.org/W2057015212","https://openalex.org/W1985671883"],"abstract_inverted_index":{"Levels":[0],"of":[1,22,39,111,137,173,210,266,313,372,390,415,428,446,515],"Reactive":[2],"Oxygen":[3],"Species":[4],"(ROS)":[5],"molecules":[6,43,498],"during":[7],"aerobic":[8],"metabolism":[9],"are":[10,423,508],"often":[11],"regulated":[12],"by":[13,121,132,157,197],"unique":[14],"endogenous":[15],"antioxidant":[16,47,429,492],"system.":[17],"During":[18],"hyperglycaemic":[19],"condition,":[20],"accumulation":[21],"excess":[23],"fatty":[24],"acids":[25],"&":[26,67,72,93,224,300,339,355,431],"glucose":[27],"in":[28,36,53,58,118,176,233,244,283,316,328,359,413,434,451,494,504,523],"adipose":[29],"tissue":[30],"(Wright":[31],"Jr":[32],"E.,":[33],"2006)":[34],"results":[35],"increased":[37],"levels":[38],"ROS.":[40],"When":[41],"ROS":[42,467],"overwhelms":[44],"the":[45,108,171,253,292,443,501,512],"cells":[46,179,230,403,422],"defence":[48],"system,":[49],"it":[50],"ends":[51],"up":[52],"cellular":[54,73],"oxidative":[55,64,435],"stress;":[56],"which":[57,499],"turn":[59],"is":[60],"reported":[61],"to":[62,70,169,376,395,441,461,510],"cause":[63],"DNA":[65,116,174,374,392,417],"damage":[66,69,117,175],"intervene":[68],"macromolecules":[71],"membranes":[74],"(Ahmad":[75],"et":[76,185],"al.,":[77,186],"2013).":[78],"Our":[79],"novel":[80],"anti-hyperglycaemic":[81,487],"polyherbal":[82,488],"formulation":[83,112,489],"(ADPHF6)":[84],"had":[85],"already":[86],"illustrated":[87],"significant":[88,411],"inhibitory":[89],"activity":[90],"against":[91,113,517],"\u03b1-amylase":[92],"\u03b1-glucosidase":[94],"enzymes":[95,430],"and":[96,152,200,242,304],"also":[97],"scavenging":[98,495],"free":[99],"radicals":[100],"(in":[101],"vitro":[102],"models).":[103],"The":[104,162,229],"present":[105,477],"study":[106,478],"demonstrates":[107],"protective":[109],"effect":[110],"H2O2":[114,319,336,356,384,409],"induced":[115],"human":[119,145],"lymphocytes":[120,146],"Single":[122],"Cell":[123,155],"Gel":[124],"Electrophoresis":[125,269],"(SCGE)":[126],"assay.":[127],"Experimental":[128],"procedures":[129],"were":[130,147,191,231,255,281,294],"approved":[131],"Institutional":[133],"Human":[134],"Ethics":[135],"Committee":[136],"Frontier":[138],"Lifeline":[139],"Hospital,":[140],"Chennai,":[141],"India":[142],"(FLL/IEC/02/2014).":[143],"Peripheral":[144],"isolated":[148],"(Duthie":[149],"et.al,":[150],"2002)":[151],"subjected":[153],"for":[154,202,236,247,261,264,275,286,466],"viability":[156],"Trypan":[158],"blue":[159],"exclusion":[160],"method.":[161],"alkaline":[163,259],"SCGE":[164,481],"assay":[165,482],"was":[166,219,270],"carried":[167],"out":[168],"determine":[170],"level":[172],"ADPHF6":[177,350,401,516],"treated":[178,256,348,380,402],"with":[180,193,221,257,296,318,335,349,381,406,425],"minor":[181],"modifications":[182],"from":[183,475],"Singh":[184],"1988.":[187],"Frosted":[188],"microscopic":[189,227],"slides":[190,254],"pre-coated":[192],"1%":[194,198],"NMA":[195],"followed":[196],"LMA":[199,223],"incubated":[201],"15":[203],"min":[204,249,277,288],"at":[205,239,250,272,278,289],"15-20o":[206],"C.":[207],"100":[208],"\u03bcL":[209],"freshly":[211],"prepared":[212],"cell":[213],"suspension":[214],"(2":[215],"x":[216],"104":[217],"cells)":[218],"mixed":[220],"0.5%":[222],"casted":[225],"on":[226,480],"slide.":[228],"immersed":[232],"lysing":[234],"solution":[235,260],"2":[237],"hours":[238],"4O":[240],"C":[241],"washed":[243,282],"TBE":[245],"buffer":[246,285],"5":[248,287],"RT.":[251,279,290],"All":[252,291],"fresh":[258],"20":[262,276],"minutes":[263],"expression":[265],"alkali-labile":[267],"damage.":[268],"performed":[271],"24":[273],"V":[274],"Slides":[280],"neutralizing":[284],"groups":[293],"stained":[295],"Acridine":[297],"Orange":[298],"(20\u00b5g/ml)":[299,303],"Propidium":[301],"Iodide":[302],"visualized":[305],"under":[306],"microscope":[307],"(Nikon":[308],"Eclipse":[309],"80i).":[310],"Reaction":[311],"mixture":[312],"Lymphocytes":[314,327,334,347],"(suspended":[315],"RPMI-1640)":[317],"(50":[320,337,341,353,357,404],"\u00b5M)":[321,338,358],"served":[322,330,343,362],"as":[323,331,344,363],"Positive":[324],"Control":[325],"(PC);":[326],"RPMI-1640":[329],"Vehicle":[332],"control;":[333],"Quercetin":[340],"mM)":[342],"Standard":[345,396],"Control.":[346],"aqueous":[351],"extract":[352],"\u00b5g/ml)":[354,405],"various":[360],"concentrations":[361],"Test":[364],"group.":[365],"In":[366],"untreated":[367],"group":[368,398],"(Vehicle":[369],"control),":[370],"%":[371,389,414],"tail":[373,391,416],"found":[375],"be":[377,462],"null.":[378],"Cells":[379],"50":[382,407],"\u00b5M":[383,408],"(Positive":[385],"control)":[386],"showed":[387,410],"maximum":[388],"(>90%)":[393],"compared":[394],"control":[397],"(<":[399,418],"30%).":[400],"decrease":[412],"20%).":[419],"Though":[420],"mammalian":[421],"equipped":[424],"impressive":[426],"range":[427],"molecules,":[432],"but":[433],"stress":[436],"conditions":[437],"these":[438],"agents":[439],"fails":[440],"normalize":[442],"redox":[444,502],"status":[445],"cell.":[447],"Various":[448],"scientific":[449],"investigations":[450],"recent":[452],"times":[453],"have":[454],"stated":[455],"that":[456],"natural":[457],"based":[458,479],"antioxidants":[459],"proved":[460],"an":[463],"alternative":[464],"therapy":[465],"mediated":[468],"effects.":[469],"To":[470],"support":[471],"this":[472],"hypothesis,":[473],"findings":[474],"our":[476,486],"clearly":[483],"suggests":[484],"that,":[485],"possesses":[490],"strong":[491],"property":[493],"Free":[496],"Radical":[497],"restore":[500],"homeostasis":[503],"cells.":[505],"Further":[506],"studies":[507],"required":[509],"confirm":[511],"potential":[513],"role":[514],"Type":[518],"II":[519],"Diabetes":[520],"Mellitus":[521],"involving":[522],"vivo":[524],"models.":[525]},"counts_by_year":[],"updated_date":"2026-11-08T12:12:28.789561","created_date":"2022-05-12T00:00:00"}