{"id":"https://openalex.org/W4235576558","doi":"https://doi.org/10.3791/1499","title":"Lentivirus Production","display_name":"Lentivirus Production","publication_year":2009,"publication_date":"2009-10-02","ids":{"openalex":"https://openalex.org/W4235576558","doi":"https://doi.org/10.3791/1499","pmid":"https://pubmed.ncbi.nlm.nih.gov/19801965","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/2865973"},"language":"en","primary_location":{"id":"doi:10.3791/1499","doi":"https://doi.org/10.3791/1499","is_oa":true,"landing_page_url":"https://doi.org/10.3791/1499","pdf_url":"https://www.jove.com/pdf/1499/lentivirus-production","source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://www.jove.com/pdf/1499/lentivirus-production","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5023471093","display_name":"Xiaoyin Wang","orcid":"https://orcid.org/0000-0002-6667-5209","observed_orcids":["https://orcid.org/0000-0002-6667-5209"]},"institutions":[{"id":"https://openalex.org/I180670191","display_name":"University of California, San Francisco","ror":"https://ror.org/043mz5j54","country_code":"US","type":"education","lineage":["https://openalex.org/I180670191"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Xiaoyin Wang","raw_affiliation_strings":["Diabetes Center, University of California, San Francisco, USA","Diabetes Center, University of California, San Francisco - UCSF"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Diabetes Center, University of California, San Francisco, USA","institution_ids":["https://openalex.org/I180670191"]},{"raw_affiliation_string":"Diabetes Center, University of California, San Francisco - UCSF","institution_ids":["https://openalex.org/I180670191"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5039780162","display_name":"Michael T. McManus","orcid":"https://orcid.org/0000-0003-3013-6569","observed_orcids":["https://orcid.org/0000-0003-3013-6569"]},"institutions":[{"id":"https://openalex.org/I180670191","display_name":"University of California, San Francisco","ror":"https://ror.org/043mz5j54","country_code":"US","type":"education","lineage":["https://openalex.org/I180670191"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Michael McManus","raw_affiliation_strings":["Diabetes Center, University of California, San Francisco - UCSF"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Diabetes Center, University of California, San Francisco - UCSF","institution_ids":["https://openalex.org/I180670191"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I180670191"],"apc_list":null,"apc_paid":null,"fwci":0.361,"has_fulltext":true,"cited_by_count":53,"citation_normalized_percentile":{"value":0.648744,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":null,"issue":"32","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.8579000234603882,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.8579000234603882,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.13510000705718994,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.0010000000474974513,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/lentivirus-production","display_name":"lentivirus production","score":0.949999988079071},{"id":"https://openalex.org/keywords/lentiviral-vectors","display_name":"lentiviral vectors","score":0.796999990940094},{"id":"https://openalex.org/keywords/rna-interference","display_name":"RNA interference","score":0.9580000042915344},{"id":"https://openalex.org/keywords/shrna","display_name":"shRNA","score":0.8199999928474426},{"id":"https://openalex.org/keywords/sirna","display_name":"siRNA","score":0.6610000133514404},{"id":"https://openalex.org/keywords/transient-transfection","display_name":"transient transfection","score":0.6919999718666077},{"id":"https://openalex.org/keywords/hek293t-cells","display_name":"HEK293T cells","score":0.824999988079071},{"id":"https://openalex.org/keywords/virus-titration","display_name":"virus titration","score":0.8130000233650208},{"id":"https://openalex.org/keywords/gene-silencing","display_name":"gene silencing","score":0.5690000057220459}],"concepts":[{"id":"https://openalex.org/C166703698","wikidata":"https://www.wikidata.org/wiki/Q201993","display_name":"RNA interference","level":4,"score":0.777290940284729},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7170019745826721},{"id":"https://openalex.org/C190232843","wikidata":"https://www.wikidata.org/wiki/Q1950808","display_name":"Small hairpin RNA","level":4,"score":0.7096954584121704},{"id":"https://openalex.org/C2779690655","wikidata":"https://www.wikidata.org/wiki/Q374232","display_name":"Lentivirus","level":4,"score":0.6444519758224487},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.6348732113838196},{"id":"https://openalex.org/C119056186","wikidata":"https://www.wikidata.org/wiki/Q1431332","display_name":"Gene silencing","level":3,"score":0.5919649004936218},{"id":"https://openalex.org/C32470452","wikidata":"https://www.wikidata.org/wiki/Q2298489","display_name":"Viral vector","level":4,"score":0.5688141584396362},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.5551328659057617},{"id":"https://openalex.org/C22615655","wikidata":"https://www.wikidata.org/wiki/Q203221","display_name":"Small interfering RNA","level":4,"score":0.5268368124961853},{"id":"https://openalex.org/C140704245","wikidata":"https://www.wikidata.org/wiki/Q3933202","display_name":"Viral replication","level":3,"score":0.5030092597007751},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.49730995297431946},{"id":"https://openalex.org/C92087593","wikidata":"https://www.wikidata.org/wiki/Q427389","display_name":"Vector (molecular biology)","level":4,"score":0.49064698815345764},{"id":"https://openalex.org/C102744134","wikidata":"https://www.wikidata.org/wiki/Q22280388","display_name":"Homologous recombination","level":3,"score":0.4780169725418091},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.43988126516342163},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.43847334384918213},{"id":"https://openalex.org/C144001008","wikidata":"https://www.wikidata.org/wiki/Q458226","display_name":"Helper virus","level":4,"score":0.4202916920185089},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3511340022087097},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.31298530101776123},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.25888147950172424},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.08643123507499695},{"id":"https://openalex.org/C2780727368","wikidata":"https://www.wikidata.org/wiki/Q1928978","display_name":"Viral disease","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D014773","descriptor_name":"Virology","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D016086","descriptor_name":"Lentivirus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D016086","descriptor_name":"Lentivirus","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":false},{"descriptor_ui":"D016086","descriptor_name":"Lentivirus","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D034741","descriptor_name":"RNA, Small Interfering","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.3791/1499","doi":"https://doi.org/10.3791/1499","is_oa":true,"landing_page_url":"https://doi.org/10.3791/1499","pdf_url":"https://www.jove.com/pdf/1499/lentivirus-production","source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:2200942","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2865973","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:2865973","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2865973","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Vis Exp","raw_type":"Text"},{"id":"pmid:19801965","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/19801965","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of visualized experiments : JoVE","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.3791/1499","doi":"https://doi.org/10.3791/1499","is_oa":true,"landing_page_url":"https://doi.org/10.3791/1499","pdf_url":"https://www.jove.com/pdf/1499/lentivirus-production","source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4235576558.pdf","grobid_xml":"https://content.openalex.org/works/W4235576558.grobid-xml"},"referenced_works_count":1,"referenced_works":["https://openalex.org/W1978335031"],"related_works":["https://openalex.org/W2328190627","https://openalex.org/W2085538564","https://openalex.org/W2065023294","https://openalex.org/W2149658494","https://openalex.org/W1967132227","https://openalex.org/W2145712751","https://openalex.org/W2393769631","https://openalex.org/W160993074","https://openalex.org/W2006350083","https://openalex.org/W2045812968"],"abstract_inverted_index":{"RNA":[0],"interference":[1],"(RNAi)":[2],"is":[3,167,174],"a":[4,44,68,92,124,135,159,181],"system":[5],"of":[6,46,79,110,122,130,140],"gene":[7],"silencing":[8],"in":[9,16,43],"living":[10],"cells.":[11,64,155],"In":[12],"RNAi,":[13],"genes":[14],"homologous":[15],"sequence":[17],"to":[18,33,58,71,76],"short":[19],"interfering":[20],"RNAs":[21],"(siRNA)":[22],"are":[23,56,89,149],"silenced":[24],"at":[25,157],"the":[26,52,77,96,111,120,131,138,163],"post-transcriptional":[27],"state.":[28],"Short":[29],"hairpin":[30],"RNAs,":[31],"precursors":[32],"siRNA,":[34],"can":[35],"be":[36],"expressed":[37],"using":[38],"lentivirus,":[39],"allowing":[40],"for":[41],"RNAi":[42],"variety":[45],"cell":[47],"types.":[48],"Lentiviruses,":[49],"such":[50],"as":[51],"Human":[53],"Immunodeficiency":[54],"Virus,":[55],"capable":[57],"infecting":[59],"both":[60],"dividing":[61],"and":[62,142,169],"non-dividing":[63],"We":[65],"will":[66],"describe":[67],"procedure":[69],"which":[70],"package":[72],"lentiviruses.":[73],"Packaging":[74],"refers":[75],"preparation":[78],"competent":[80],"virus":[81,126,164,172],"from":[82],"DNA":[83],"vectors.":[84],"Lentiviral":[85],"vector":[86,136],"production":[87],"systems":[88],"based":[90],"on":[91],"'split'":[93],"system,":[94],"where":[95],"natural":[97],"viral":[98,113],"genome":[99],"has":[100],"been":[101],"split":[102],"into":[103,115,152],"individual":[104],"helper":[105],"plasmid":[106],"constructs.":[107],"This":[108],"splitting":[109],"different":[112],"elements":[114],"four":[116],"separate":[117],"vectors":[118,145],"diminishes":[119],"risk":[121],"creating":[123],"replication-capable":[125],"by":[127,176],"adventitious":[128],"recombination":[129],"lentiviral":[132],"genome.":[133],"Here,":[134],"containing":[137,165],"shRNA":[139],"interest":[141],"three":[143],"packaging":[144],"(p-VSVG,":[146],"pRSV,":[147],"pMDL)":[148],"transiently":[150],"transfected":[151],"human":[153],"293":[154],"After":[156],"least":[158],"48-hour":[160],"incubation":[161],"period,":[162],"supernatant":[166],"harvested":[168],"concentrated.":[170],"Finally,":[171],"titer":[173],"determined":[175],"reporter":[177],"(fluorescent)":[178],"expression":[179],"with":[180],"flow":[182],"cytometer.":[183]},"counts_by_year":[{"year":2026,"cited_by_count":3},{"year":2025,"cited_by_count":2},{"year":2023,"cited_by_count":3},{"year":2022,"cited_by_count":5},{"year":2021,"cited_by_count":5},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":4},{"year":2017,"cited_by_count":6},{"year":2016,"cited_by_count":8},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":4},{"year":2013,"cited_by_count":6}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2022-05-12T00:00:00"}
