{"id":"https://openalex.org/W4232299219","doi":"https://doi.org/10.1385/0-89603-332-5:279","title":"Construction of Linker-Scanning Mutations by Oligonucleotide Ligation","display_name":"Construction of Linker-Scanning Mutations by Oligonucleotide Ligation","publication_year":2003,"publication_date":"2003-11-14","ids":{"openalex":"https://openalex.org/W4232299219","doi":"https://doi.org/10.1385/0-89603-332-5:279"},"language":"en","primary_location":{"id":"doi:10.1385/0-89603-332-5:279","doi":"https://doi.org/10.1385/0-89603-332-5:279","is_oa":false,"landing_page_url":"https://doi.org/10.1385/0-89603-332-5:279","pdf_url":null,"source":{"id":"https://openalex.org/S4306463399","display_name":"Humana Press eBooks","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310315801","host_organization_name":"Humana Press","host_organization_lineage":["https://openalex.org/P4310315801","https://openalex.org/P4310319900"],"host_organization_lineage_names":["Humana Press","Springer Science+Business Media"],"type":"ebook platform"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"In Vitro Mutagenesis Protocols","raw_type":"book-chapter"},"volume":{"id":"https://openalex.org/W563419041","display_name":"In Vitro Mutagenesis Protocols"},"type":"book-chapter","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5091066839","display_name":"Grace M. Hobson","orcid":"https://orcid.org/0000-0002-5275-5953","observed_orcids":["https://orcid.org/0000-0002-5275-5953"]},"institutions":[{"id":"https://openalex.org/I1316910977","display_name":"DuPont (United States)","ror":"https://ror.org/03g3m1w62","country_code":"US","type":"company","lineage":["https://openalex.org/I1316910977"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Grace M. Hobson","raw_affiliation_strings":["Research and Development, DuPont Merck, Wilmington, DE, USA","Research and Development, DuPont Merck, Wilmington"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Research and Development, DuPont Merck, Wilmington, DE, USA","institution_ids":["https://openalex.org/I1316910977"]},{"raw_affiliation_string":"Research and Development, DuPont Merck, Wilmington","institution_ids":["https://openalex.org/I1316910977"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5079767131","display_name":"Patricia P. Harlow","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1316910977","display_name":"DuPont (United States)","ror":"https://ror.org/03g3m1w62","country_code":"US","type":"company","lineage":["https://openalex.org/I1316910977"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Patricia P. Harlow","raw_affiliation_strings":["Research and Development, DuPont Merck, Wilmington"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Research and Development, DuPont Merck, Wilmington","institution_ids":["https://openalex.org/I1316910977"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111454776","display_name":"Pamela A. Benfield","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1316910977","display_name":"DuPont (United States)","ror":"https://ror.org/03g3m1w62","country_code":"US","type":"company","lineage":["https://openalex.org/I1316910977"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Pamela A. Benfield","raw_affiliation_strings":["Research and Development, DuPont Merck, Wilmington"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Research and Development, DuPont Merck, Wilmington","institution_ids":["https://openalex.org/I1316910977"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I1316910977"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":{"value":0.40179084,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":"279","last_page":"286"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.48100000619888306,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.48100000619888306,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.23109999299049377,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.14890000224113464,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/oligonucleotide-ligation","display_name":"oligonucleotide ligation","score":0.9890000224113464},{"id":"https://openalex.org/keywords/site-directed-mutagenesis","display_name":"site-directed mutagenesis","score":0.8930000066757202},{"id":"https://openalex.org/keywords/cis-regulatory-elements","display_name":"cis-regulatory elements","score":0.8870000243186951},{"id":"https://openalex.org/keywords/protein-mutants","display_name":"protein mutants","score":0.5270000100135803}],"concepts":[{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.8072133660316467},{"id":"https://openalex.org/C2780557392","wikidata":"https://www.wikidata.org/wiki/Q523796","display_name":"Linker","level":2,"score":0.7222020626068115},{"id":"https://openalex.org/C16318435","wikidata":"https://www.wikidata.org/wiki/Q149299","display_name":"Mutagenesis","level":4,"score":0.5898886919021606},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.5388903021812439},{"id":"https://openalex.org/C129312508","wikidata":"https://www.wikidata.org/wiki/Q133686","display_name":"Oligonucleotide","level":3,"score":0.47437340021133423},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.4533774256706238},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.4461447596549988},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.41289931535720825},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3655688166618347},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.1173558235168457},{"id":"https://openalex.org/C111919701","wikidata":"https://www.wikidata.org/wiki/Q9135","display_name":"Operating system","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1385/0-89603-332-5:279","doi":"https://doi.org/10.1385/0-89603-332-5:279","is_oa":false,"landing_page_url":"https://doi.org/10.1385/0-89603-332-5:279","pdf_url":null,"source":{"id":"https://openalex.org/S4306463399","display_name":"Humana Press eBooks","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310315801","host_organization_name":"Humana Press","host_organization_lineage":["https://openalex.org/P4310315801","https://openalex.org/P4310319900"],"host_organization_lineage_names":["Humana Press","Springer Science+Business Media"],"type":"ebook platform"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"In Vitro Mutagenesis Protocols","raw_type":"book-chapter"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4388384806","https://openalex.org/W2474375822","https://openalex.org/W2405423149","https://openalex.org/W2413426677","https://openalex.org/W2287032645","https://openalex.org/W1580605464","https://openalex.org/W2027286798","https://openalex.org/W2010338968","https://openalex.org/W2466963159","https://openalex.org/W2415625684"],"abstract_inverted_index":{"The":[0,54,65,81,147],"purpose":[1],"of":[2,10,48,52,77,114,133,136],"linker-scanning":[3,137],"mutagenesis":[4],"is":[5,67,85],"to":[6,35,43,94],"create":[7],"a":[8,134,140,159],"series":[9,135],"mutant":[11,24,161],"molecules":[12],"in":[13,87,120,125,139],"which":[14],"individual":[15],"sections":[16],"are":[17,100],"sequentially":[18],"replaced":[19],"with":[20],"the":[21,30,45,75,97,109,121,151,155],"same":[22,82],"\u201cneutral\u201d":[23],"sequence":[25,153],"(see":[26],"Fig.":[27,128],"1).":[28],"Typically":[29],"technique":[31,55],"has":[32,38,56],"been":[33,41],"applied":[34],"DNA":[36,144],"and":[37,69,105,111,154],"most":[39],"commonly":[40],"used":[42],"determine":[44],"cis-regulatory":[46],"roles":[47],"upstream":[49],"flanking":[50],"regions":[51],"genes.":[53],"several":[57],"advantages":[58],"over":[59],"more":[60],"random":[61],"mutational":[62],"analysis:":[63],"1.":[64,129],"approach":[66],"systematic":[68],"implies":[70],"no":[71],"previous":[72],"prejudice":[73],"about":[74],"location":[76],"functional":[78,116],"elements;":[79],"2.":[80],"mutated":[83,98],"segment":[84,99],"substituted":[86],"each":[88],"mutant,":[89],"so":[90],"that":[91],"potential":[92],"contributions":[93],"activity":[95],"from":[96],"minimized":[101],"when":[102],"comparing":[103],"mutants;":[104],"3.":[106],"Most":[107],"importantly,":[108],"spacing":[110],"torsional":[112],"orientation":[113],"remaining":[115],"elements":[117],"remains":[118],"unchanged":[119],"mutants.":[122],"Open":[123],"image":[124],"new":[126],"window":[127],"A":[130],"schematic":[131],"representation":[132],"mutants":[138],"biopolymer":[141],"such":[142],"as":[143],"or":[145],"protein.":[146],"open":[148],"boxes":[149,157],"represent":[150,158],"wild-type":[152],"hatched":[156],"replacement":[160],"segment.":[162]},"counts_by_year":[],"updated_date":"2026-10-10T09:11:00.788812","created_date":"2022-05-12T00:00:00"}