{"id":"https://openalex.org/W4226463556","doi":"https://doi.org/10.1097/ju.0000000000002538.05","title":"PD12-05 SPATIAL TRANSCRIPTOMICS PROVIDES EVIDENCE FOR ALTERNATIVE CHECKPOINT AXES IN BCG-TREATED NMIBC","display_name":"PD12-05 SPATIAL TRANSCRIPTOMICS PROVIDES EVIDENCE FOR ALTERNATIVE CHECKPOINT AXES IN BCG-TREATED NMIBC","publication_year":2022,"publication_date":"2022-04-07","ids":{"openalex":"https://openalex.org/W4226463556","doi":"https://doi.org/10.1097/ju.0000000000002538.05"},"language":"en","primary_location":{"id":"doi:10.1097/ju.0000000000002538.05","doi":"https://doi.org/10.1097/ju.0000000000002538.05","is_oa":false,"landing_page_url":"https://doi.org/10.1097/ju.0000000000002538.05","pdf_url":null,"source":{"id":"https://openalex.org/S30525748","display_name":"The Journal of Urology","issn_l":"0022-5347","issn":["0022-5347","1527-3792"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315671","host_organization_name":"Lippincott Williams & Wilkins","host_organization_lineage":["https://openalex.org/P4310315671","https://openalex.org/P4310318547"],"host_organization_lineage_names":["Lippincott Williams & Wilkins","Wolters Kluwer"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Urology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5008586821","display_name":"Daniel L Ranti","orcid":"https://orcid.org/0000-0002-8310-1063","observed_orcids":["https://orcid.org/0000-0002-8310-1063"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Daniel Ranti","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5066731530","display_name":"Yuanshuo Wang","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Yuanshuo Wang","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5084732765","display_name":"Jorge Daza","orcid":"https://orcid.org/0000-0002-5476-3041","observed_orcids":["https://orcid.org/0000-0002-5476-3041"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Jorge Daza","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5007683158","display_name":"Sanjana Shroff","orcid":"https://orcid.org/0000-0003-2839-0322","observed_orcids":["https://orcid.org/0000-0003-2839-0322"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Sanjana Shroff","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5026912903","display_name":"Kristin G. Beaumont","orcid":"https://orcid.org/0000-0003-3075-9977","observed_orcids":["https://orcid.org/0000-0003-3075-9977"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Kristin Beaumont","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5083361833","display_name":"Amir Horowitz","orcid":"https://orcid.org/0000-0003-3100-2810","observed_orcids":["https://orcid.org/0000-0003-3100-2810","https://orcid.org/0000-0003-4602-1440"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Amir Horowitz","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5010082766","display_name":"John P. Sfakianos","orcid":"https://orcid.org/0000-0002-3101-0266","observed_orcids":["https://orcid.org/0000-0002-3101-0266"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"John Sfakianos","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3000,"currency":"USD","value_usd":3000},"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.08901381,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"207","issue":"Supplement 5","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10458","display_name":"Bladder and Urothelial Cancer Treatments","score":0.7099999785423279,"subfield":{"id":"https://openalex.org/subfields/2746","display_name":"Surgery"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10458","display_name":"Bladder and Urothelial Cancer Treatments","score":0.7099999785423279,"subfield":{"id":"https://openalex.org/subfields/2746","display_name":"Surgery"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10158","display_name":"Cancer Immunotherapy and Biomarkers","score":0.27149999141693115,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10279","display_name":"Urinary Bladder and Prostate Research","score":0.0007999999797903001,"subfield":{"id":"https://openalex.org/subfields/2748","display_name":"Urology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/non-muscle-invasive-bladder-cancer","display_name":"non-muscle-invasive bladder cancer","score":0.9769999980926514},{"id":"https://openalex.org/keywords/pd-1-pd-l1-axis","display_name":"PD-1/PD-L1 axis","score":0.8339999914169312},{"id":"https://openalex.org/keywords/immune-checkpoint-inhibitor-resistance","display_name":"immune checkpoint inhibitor resistance","score":0.7900000214576721},{"id":"https://openalex.org/keywords/nk-cell-exhaustion","display_name":"NK cell exhaustion","score":0.5379999876022339},{"id":"https://openalex.org/keywords/cd8-t-cells","display_name":"CD8+ T cells","score":0.7910000085830688},{"id":"https://openalex.org/keywords/tumor-microenvironment","display_name":"tumor microenvironment","score":0.6940000057220459},{"id":"https://openalex.org/keywords/spatial-transcriptomics","display_name":"spatial transcriptomics","score":0.7670000195503235},{"id":"https://openalex.org/keywords/tumor-stroma","display_name":"tumor stroma","score":0.6990000009536743},{"id":"https://openalex.org/keywords/tumor-infiltrating-immune-cells","display_name":"tumor-infiltrating immune cells","score":0.6679999828338623},{"id":"https://openalex.org/keywords/bladder-cancer-recurrence","display_name":"bladder cancer recurrence","score":0.6790000200271606}],"concepts":[{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.7200063467025757},{"id":"https://openalex.org/C2780851360","wikidata":"https://www.wikidata.org/wiki/Q21686041","display_name":"Immune checkpoint","level":4,"score":0.6072877049446106},{"id":"https://openalex.org/C162317418","wikidata":"https://www.wikidata.org/wiki/Q252857","display_name":"Transcriptome","level":4,"score":0.5552866458892822},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.4935256242752075},{"id":"https://openalex.org/C167672396","wikidata":"https://www.wikidata.org/wiki/Q417800","display_name":"CD8","level":3,"score":0.4810360074043274},{"id":"https://openalex.org/C2780352672","wikidata":"https://www.wikidata.org/wiki/Q504775","display_name":"Bladder cancer","level":3,"score":0.41626936197280884},{"id":"https://openalex.org/C143998085","wikidata":"https://www.wikidata.org/wiki/Q162555","display_name":"Oncology","level":1,"score":0.361160546541214},{"id":"https://openalex.org/C8891405","wikidata":"https://www.wikidata.org/wiki/Q1059","display_name":"Immune system","level":2,"score":0.3205626606941223},{"id":"https://openalex.org/C2777701055","wikidata":"https://www.wikidata.org/wiki/Q1427096","display_name":"Immunotherapy","level":3,"score":0.3024856746196747},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.2986677587032318},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.261261910200119},{"id":"https://openalex.org/C121608353","wikidata":"https://www.wikidata.org/wiki/Q12078","display_name":"Cancer","level":2,"score":0.232930988073349},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.14097702503204346},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.11713367700576782},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.0},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1097/ju.0000000000002538.05","doi":"https://doi.org/10.1097/ju.0000000000002538.05","is_oa":false,"landing_page_url":"https://doi.org/10.1097/ju.0000000000002538.05","pdf_url":null,"source":{"id":"https://openalex.org/S30525748","display_name":"The Journal of Urology","issn_l":"0022-5347","issn":["0022-5347","1527-3792"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315671","host_organization_name":"Lippincott Williams & Wilkins","host_organization_lineage":["https://openalex.org/P4310315671","https://openalex.org/P4310318547"],"host_organization_lineage_names":["Lippincott Williams & Wilkins","Wolters Kluwer"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Urology","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.9537000060081482,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4362595233","https://openalex.org/W2892779818","https://openalex.org/W4313369199","https://openalex.org/W4413297329","https://openalex.org/W3212881880","https://openalex.org/W4402164056","https://openalex.org/W4214929015","https://openalex.org/W4306884778","https://openalex.org/W3127737538","https://openalex.org/W4414543331"],"abstract_inverted_index":{"You":[0],"have":[1],"accessJournal":[2],"of":[3,122,140,161,235,259,293,334,346,353,365,379,394,397,409,416,429,456,464],"UrologyCME1":[4],"May":[5],"2022PD12-05":[6],"SPATIAL":[7],"TRANSCRIPTOMICS":[8],"PROVIDES":[9],"EVIDENCE":[10],"FOR":[11],"ALTERNATIVE":[12],"CHECKPOINT":[13],"AXES":[14],"IN":[15],"BCG-TREATED":[16],"NMIBC":[17,206],"Daniel":[18,33,497],"Ranti,":[19],"Yuanshuo":[20,42,504],"Wang,":[21],"Jorge":[22,51,511],"Daza,":[23],"Sanjana":[24,60,518],"Shroff,":[25],"Kristin":[26,69,525],"Beaumont,":[27],"Amir":[28,78,532],"Horowitz,":[29],"and":[30,87,150,163,178,181,190,211,274,283,287,297,319,336,341,399,431,435,474,493],"John":[31,88,539],"Sfakianos":[32,90,540],"RantiDaniel":[34],"Ranti":[35,498],"More":[36,45,54,63,72,81,91,499,506,513,520,527,534,541],"articles":[37,46,55,64,73,82,92,500,507,514,521,528,535,542],"by":[38,47,56,65,74,83,93,223,384,421,469,488,501,508,515,522,529,536,543],"this":[39,48,57,66,75,84,94,502,509,516,523,530,537,544],"author":[40,49,58,67,76,85,95,503,510,517,524,531,538,545],",":[41,50,59,68,77,86],"WangYuanshuo":[43],"Wang":[44,505],"DazaJorge":[52],"Daza":[53,512],"ShroffSanjana":[61],"Shroff":[62,519],"BeaumontKristin":[70],"Beaumont":[71,526],"HorowitzAmir":[79],"Horowitz":[80,533],"SfakianosJohn":[89],"View":[96],"All":[97,547],"Author":[98],"Informationhttps://doi.org/10.1097/JU.0000000000002538.05AboutPDF":[99],"ToolsAdd":[100],"to":[101,172,182,214,312],"favoritesDownload":[102],"CitationsTrack":[103],"CitationsPermissionsReprints":[104],"ShareFacebookLinked":[105],"InTwitterEmail":[106],"Abstract":[107],"INTRODUCTION":[108],"AND":[109],"OBJECTIVE:":[110],"Recurrence":[111],"in":[112,146,158,273,392],"BCG":[113,457],"treated":[114],"non-muscle":[115],"invasive":[116],"bladder":[117],"cancer":[118],"is":[119,144,433,442],"significant.":[120],"Disruption":[121],"the":[123,184,215,249,257,314,329],"PD-1:PD-L1":[124],"axis":[125,455],"via":[126,459],"checkpoint":[127,157],"inhibitors":[128],"has":[129],"shown":[130],"promise,":[131],"however":[132],"studies":[133],"show":[134],"PD-L1":[135,432],"resistance":[136,458],"may":[137],"explain":[138],"\u223c25%":[139],"recurrence.":[141],"Antitumor":[142],"immunity":[143],"critical":[145],"clearing":[147],"recurrent":[148],"tumors,":[149],"evidence":[151],"supports":[152],"HLA-E/NKG2A":[153],"as":[154,241,254,256,370],"a":[155,201,236,452],"key":[156],"NK":[159,189,260,282,335,358,385,422,460],"clearance":[160],"tumors":[162,350],"CD8":[164,284,337,360,387,424],"T-cell":[165],"infiltration.":[166],"We":[167,269,355],"used":[168],"spatial":[169],"sequencing":[170],"(STseq)":[171],"visualize":[173],"interactions":[174,437],"between":[175,316,438],"tumor,":[176],"stroma,":[177],"immune":[179,271,317,417,440],"cells,":[180,263],"investigate":[183],"molecular":[185],"signatures":[186,340],"associated":[187],"with":[188,194,232,245],"T":[191,262,285,338,361,388,425],"cells":[192,272,278,327,362,389,426,441],"interacting":[193],"tumor.":[195],"METHODS:":[196],"STseq":[197],"was":[198,310,390],"performed":[199,311],"on":[200],"specimen":[202],"from":[203],"1":[204],"BCG-resistant":[205],"case.":[207],"Slides":[208],"were":[209,221,239,264,299],"processed":[210],"imaged":[212],"according":[213],"Visium":[216,230],"10X":[217],"protocol.":[218],"Cell":[219],"lineages":[220,253,321],"defined":[222],"Robertson":[224],"AG":[225],"et":[226],"al.":[227],"(Cell.":[228],"2017).":[229],"spots":[231],">90th":[233],"percentile":[234],"linage":[237],"signature":[238],"labelled":[240],"high-signature":[242],"spots.":[243],"Spots":[244],"multiple":[246],"signatures,":[247],"representing":[248],"plasticity":[250],"across":[251],"tumor":[252,277,288,320,326,381,418,439],"well":[255],"presence":[258],"or":[261],"annotated":[265],"accordingly":[266],"(Fig.":[267,301,322],"1A).":[268],"identified":[270],"immediately":[275],"surrounding":[276],"using":[279],"NK,":[280],"mutual":[281],"cell,":[286],"cell":[289,339,382,419],"markers.":[290],"Combined":[291],"score":[292],"all":[294],"listed":[295],"genes":[296],"HLA-E":[298,398,430,449],"visualized":[300],"1B).":[302],"A":[303],"differential":[304],"gene":[305],"expression":[306,352,396,428],"analysis,":[307],"scaled":[308],"column-wise,":[309],"understand":[313],"relationship":[315],"markers":[318],"1C).":[323],"RESULTS:":[324],"Neuronal":[325],"had":[328],"second":[330],"highest":[331],"observed":[332,357],"co-expression":[333],"similarly":[342],"expressed":[343],"high":[344],"levels":[345],"HLA-E.":[347],"However,":[348],"\u201cneuronal\u201d":[349],"lacked":[351],"PD-L1.":[354],"also":[356],"and/or":[359,386,423],"infiltrating":[363],"nests":[364,383,420],"other":[366],"lineage":[367],"subtypes,":[368],"such":[369],"\u201cp53\u201d,":[371],"but":[372],"lacking":[373],"activation":[374],"signals.":[375],"Lastly,":[376],"preferential":[377,414],"infiltration":[378,415],"\u201cimmune\u201d":[380],"seen":[391],"areas":[393],"higher":[395],"CD274":[400],"(PD-L1).":[401],"CONCLUSIONS:":[402],"These":[403],"results":[404],"suggest":[405],"overlapping":[406],"non-redundant":[407],"mechanisms":[408],"evasion.":[410],"Our":[411],"analysis":[412],"confirms":[413],"where":[427],"higher,":[434],"that":[436,448],"heterogeneous":[443],"within":[444],"samples.":[445],"This":[446],"suggests":[447],"could":[450],"be":[451],"secondary":[453],"inhibitory":[454],"mediated":[461],"HLA-E:NKG2A.":[462],"Source":[463],"Funding:":[465],"NA":[466],"\u00a9":[467],"2022":[468,487],"American":[470,489],"Urological":[471,490],"Association":[472,491],"Education":[473,492],"Research,":[475,494],"Inc.FiguresReferencesRelatedDetails":[476],"Volume":[477],"207Issue":[478],"Supplement":[479],"5May":[480],"2022Page:":[481],"e196":[482],"Advertisement":[483,548],"Copyright":[484],"&":[485],"Permissions\u00a9":[486],"Inc.MetricsAuthor":[495],"Information":[496],"Expand":[546],"PDF":[549],"DownloadLoading":[550],"...":[551]},"counts_by_year":[],"updated_date":"2026-10-09T09:14:12.587421","created_date":"2022-05-05T00:00:00"}