{"id":"https://openalex.org/W4214836719","doi":"https://doi.org/10.1016/j.jbc.2022.101802","title":"The Escherichia coli outer membrane protein OmpA acquires secondary structure prior to its integration into the membrane","display_name":"The Escherichia coli outer membrane protein OmpA acquires secondary structure prior to its integration into the membrane","publication_year":2022,"publication_date":"2022-03-04","ids":{"openalex":"https://openalex.org/W4214836719","doi":"https://doi.org/10.1016/j.jbc.2022.101802","pmid":"https://pubmed.ncbi.nlm.nih.gov/35257747","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/8987393"},"language":"en","primary_location":{"id":"doi:10.1016/j.jbc.2022.101802","doi":"https://doi.org/10.1016/j.jbc.2022.101802","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.jbc.2022.101802","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"parent_works":[],"lineage":["https://openalex.org/W4214836719"],"child_works_count":0,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.1016/j.jbc.2022.101802","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5014462572","display_name":"Xu Wang","orcid":"https://orcid.org/0000-0003-1309-1848","observed_orcids":["https://orcid.org/0000-0003-1309-1848"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210090567","display_name":"National Institute of Diabetes and Digestive and Kidney Diseases","ror":"https://ror.org/00adh9b73","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210090567"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Xu Wang","raw_affiliation_strings":["Genetics and Biochemistry Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA"],"raw_orcid":"https://orcid.org/0000-0003-1309-1848","affiliations":[{"raw_affiliation_string":"Genetics and Biochemistry Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210090567"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5062522223","display_name":"Harris D. Bernstein","orcid":"https://orcid.org/0000-0002-4941-3741","observed_orcids":["https://orcid.org/0000-0002-4941-3741"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210090567","display_name":"National Institute of Diabetes and Digestive and Kidney Diseases","ror":"https://ror.org/00adh9b73","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210090567"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Harris D. Bernstein","raw_affiliation_strings":["Genetics and Biochemistry Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA. Electronic address: harris_bernstein@nih.gov","Genetics and Biochemistry Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA"],"raw_orcid":"https://orcid.org/0000-0002-4941-3741","affiliations":[{"raw_affiliation_string":"Genetics and Biochemistry Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA. Electronic address: harris_bernstein@nih.gov","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210090567"]},{"raw_affiliation_string":"Genetics and Biochemistry Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210090567"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5062522223"],"corresponding_institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210090567"],"apc_list":{"value":2500,"currency":"USD","value_usd":2500},"apc_paid":{"value":2500,"currency":"USD","value_usd":2500},"fwci":1.9424,"has_fulltext":true,"cited_by_count":21,"citation_normalized_percentile":{"value":0.86122862,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":93,"max":98},"biblio":{"volume":"298","issue":"4","first_page":"101802","last_page":"101802"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9761000275611877,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9761000275611877,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.009600000455975533,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10407","display_name":"Lipid Membrane Structure and Behavior","score":0.004999999888241291,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/ompa","display_name":"OmpA","score":0.9769999980926514},{"id":"https://openalex.org/keywords/outer-membrane-proteins","display_name":"outer membrane proteins","score":0.8320000171661377},{"id":"https://openalex.org/keywords/protein-biogenesis","display_name":"protein biogenesis","score":0.6600000262260437},{"id":"https://openalex.org/keywords/disulfide-bonds","display_name":"disulfide bonds","score":0.7630000114440918},{"id":"https://openalex.org/keywords/split-proteins","display_name":"split proteins","score":0.5960000157356262},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.8489999771118164},{"id":"https://openalex.org/keywords/membrane-insertion","display_name":"membrane insertion","score":0.5400000214576721}],"concepts":[{"id":"https://openalex.org/C201663137","wikidata":"https://www.wikidata.org/wiki/Q21198990","display_name":"Periplasmic space","level":4,"score":0.9195763468742371},{"id":"https://openalex.org/C146587185","wikidata":"https://www.wikidata.org/wiki/Q258248","display_name":"Bacterial outer membrane","level":4,"score":0.8880150318145752},{"id":"https://openalex.org/C131934819","wikidata":"https://www.wikidata.org/wiki/Q135390","display_name":"Biogenesis","level":3,"score":0.671389102935791},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.6651873588562012},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.5356281399726868},{"id":"https://openalex.org/C172227984","wikidata":"https://www.wikidata.org/wiki/Q1400397","display_name":"Barrel (horology)","level":2,"score":0.5184428691864014},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.44169676303863525},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.4262647032737732},{"id":"https://openalex.org/C2779201268","wikidata":"https://www.wikidata.org/wiki/Q186474","display_name":"Cysteine","level":3,"score":0.42324990034103394},{"id":"https://openalex.org/C47701112","wikidata":"https://www.wikidata.org/wiki/Q735188","display_name":"Protein structure","level":2,"score":0.41430097818374634},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3957037031650543},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.395454466342926},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.08815151453018188},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.0},{"id":"https://openalex.org/C159985019","wikidata":"https://www.wikidata.org/wiki/Q181790","display_name":"Composite material","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000072757","descriptor_name":"Protein Conformation, beta-Strand","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001425","descriptor_name":"Bacterial Outer Membrane Proteins","qualifier_ui":"Q000138","qualifier_name":"chemical synthesis","is_major_topic":true},{"descriptor_ui":"D001425","descriptor_name":"Bacterial Outer Membrane Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D002462","descriptor_name":"Cell Membrane","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004220","descriptor_name":"Disulfides","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D017433","descriptor_name":"Protein Structure, Secondary","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017510","descriptor_name":"Protein Folding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1016/j.jbc.2022.101802","doi":"https://doi.org/10.1016/j.jbc.2022.101802","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.jbc.2022.101802","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:8987393","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC8987393/","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC8987393/pdf/","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Biol Chem","raw_type":"Text"},{"id":"pmid:35257747","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/35257747","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1016/j.jbc.2022.101802","doi":"https://doi.org/10.1016/j.jbc.2022.101802","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.jbc.2022.101802","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337357","display_name":"National Institute of Diabetes and Digestive and Kidney Diseases","ror":"https://ror.org/00adh9b73"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4214836719.pdf","grobid_xml":"https://content.openalex.org/works/W4214836719.grobid-xml"},"referenced_works_count":53,"referenced_works":["https://openalex.org/W291478791","https://openalex.org/W1558934115","https://openalex.org/W1677443113","https://openalex.org/W1754378627","https://openalex.org/W1816389847","https://openalex.org/W1944838415","https://openalex.org/W1968522752","https://openalex.org/W1971439989","https://openalex.org/W1980858544","https://openalex.org/W1982704053","https://openalex.org/W1990365720","https://openalex.org/W1995411885","https://openalex.org/W2004696942","https://openalex.org/W2014101652","https://openalex.org/W2019381852","https://openalex.org/W2029664798","https://openalex.org/W2044784863","https://openalex.org/W2049387326","https://openalex.org/W2055341880","https://openalex.org/W2060719766","https://openalex.org/W2076974004","https://openalex.org/W2081717721","https://openalex.org/W2089308394","https://openalex.org/W2095139984","https://openalex.org/W2099548197","https://openalex.org/W2107516741","https://openalex.org/W2118991108","https://openalex.org/W2142267788","https://openalex.org/W2172287485","https://openalex.org/W2235470599","https://openalex.org/W2275287916","https://openalex.org/W2282925852","https://openalex.org/W2525566551","https://openalex.org/W2557215068","https://openalex.org/W2589042007","https://openalex.org/W2621269786","https://openalex.org/W2755227656","https://openalex.org/W2766806298","https://openalex.org/W2787617893","https://openalex.org/W2885624582","https://openalex.org/W2899749851","https://openalex.org/W2963895641","https://openalex.org/W2965863233","https://openalex.org/W2989562929","https://openalex.org/W3033772165","https://openalex.org/W3037734473","https://openalex.org/W3135257560","https://openalex.org/W3152535485","https://openalex.org/W3159422309","https://openalex.org/W3177828909","https://openalex.org/W3211795435","https://openalex.org/W4200270387","https://openalex.org/W4221048674"],"related_works":["https://openalex.org/W291478791","https://openalex.org/W1992819425","https://openalex.org/W4367049040","https://openalex.org/W1560081729","https://openalex.org/W2065227810","https://openalex.org/W2141375922","https://openalex.org/W2603812880","https://openalex.org/W2049387326","https://openalex.org/W7202265205","https://openalex.org/W1978312435"],"abstract_inverted_index":{"Almost":[0],"all":[1],"proteins":[2],"that":[3,17,54,82,162,177,206],"reside":[4],"in":[5,68,89,97,103,125,132,150,158],"the":[6,28,46,55,90,98,104,110,120,133,151,159,192,200],"outer":[7,39],"membrane":[8,29,40],"(OM)":[9],"of":[10,112,114,203],"Gram-negative":[11],"bacteria":[12],"contain":[13],"a":[14,20,65,165,184],"membrane-spanning":[15],"segment":[16],"folds":[18],"into":[19,27,38,64,183,191],"unique":[21],"\u03b2":[22,59,86,94,136,147,155,170,178],"barrel":[23,60],"structure":[24,67,186],"and":[25,93,107,154],"inserts":[26],"by":[30],"an":[31],"unknown":[32],"mechanism.":[33],"To":[34],"obtain":[35],"further":[36],"insight":[37],"protein":[41],"(OMP)":[42],"biogenesis,":[43],"we":[44,80,140],"revisited":[45],"surprising":[47],"observation":[48],"reported":[49],"over":[50],"20":[51],"years":[52],"ago":[53],"Escherichia":[56],"coli":[57],"OmpA":[58],"can":[61,101],"be":[62],"assembled":[63],"native":[66],"vivo":[69],"when":[70],"it":[71],"is":[72],"expressed":[73],"as":[74],"two":[75],"noncovalently":[76],"linked":[77],"fragments.":[78],"Here,":[79],"show":[81],"disulfide":[83,144],"bonds":[84],"between":[85,146],"strand":[87,95,148,156],"4":[88],"N-terminal":[91,152],"fragment":[92,100,153,161],"5":[96],"C-terminal":[99,160],"form":[102,164],"periplasmic":[105],"space":[106],"greatly":[108],"increase":[109],"efficiency":[111],"assembly":[113],"\"split\"":[115],"OmpA,":[116],"but":[117,194],"only":[118,142,175],"if":[119],"cysteine":[121],"residues":[122],"are":[123,130,189],"engineered":[124],"perfect":[126],"register":[127],"(i.e.,":[128],"they":[129,188],"aligned":[131],"fully":[134],"folded":[135],"barrel).":[137],"In":[138],"contrast,":[139],"observed":[141],"weak":[143],"bonding":[145],"1":[149],"8":[157],"would":[163],"closed":[166],"or":[167],"circularly":[168],"permutated":[169],"barrel.":[171],"Our":[172],"results":[173],"not":[174],"demonstrate":[176],"barrels":[179],"begin":[180],"to":[181,197],"fold":[182],"\u03b2-sheet-like":[185],"before":[187],"integrated":[190],"OM":[193],"also":[195],"help":[196],"discriminate":[198],"among":[199],"different":[201],"models":[202],"OMP":[204],"biogenesis":[205],"have":[207],"been":[208],"proposed.":[209]},"counts_by_year":[{"year":2026,"cited_by_count":5},{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":6},{"year":2023,"cited_by_count":5},{"year":2022,"cited_by_count":2}],"updated_date":"2026-11-13T13:42:25.865203","created_date":"2022-03-05T00:00:00"}
