{"id":"https://openalex.org/W4211243875","doi":"https://doi.org/10.17504/protocols.io.b4pgqvjw","title":"Preparing MEF-cultured hPSCs for nucleofection v1","display_name":"Preparing MEF-cultured hPSCs for nucleofection v1","publication_year":2022,"publication_date":"2022-02-03","ids":{"openalex":"https://openalex.org/W4211243875","doi":"https://doi.org/10.17504/protocols.io.b4pgqvjw"},"language":"en","primary_location":{"id":"doi:10.17504/protocols.io.b4pgqvjw","doi":"https://doi.org/10.17504/protocols.io.b4pgqvjw","is_oa":true,"landing_page_url":"https://doi.org/10.17504/protocols.io.b4pgqvjw","pdf_url":null,"source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://doi.org/10.17504/protocols.io.b4pgqvjw","any_repository_has_fulltext":null},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5038944590","display_name":"Hanqin Li","orcid":"https://orcid.org/0000-0001-7995-1084","observed_orcids":["https://orcid.org/0000-0001-7995-1084","https://orcid.org/0009-0008-3334-6899"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Hanqin Li","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0001-7995-1084","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5000495585","display_name":"Oriol Busquets","orcid":"https://orcid.org/0000-0002-1372-9699","observed_orcids":["https://orcid.org/0000-0002-1372-9699"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Oriol Busquets","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-1372-9699","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5082161278","display_name":"Steven Poser","orcid":"https://orcid.org/0000-0003-4868-1389","observed_orcids":["https://orcid.org/0000-0003-4868-1389"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Steven Poser","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0003-4868-1389","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5045835078","display_name":"Dirk F. Hockemeyer","orcid":"https://orcid.org/0000-0002-5598-5092","observed_orcids":["https://orcid.org/0000-0002-5598-5092"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Dirk Hockemeyer","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-5598-5092","affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5070152031","display_name":"Frank Soldner","orcid":"https://orcid.org/0000-0002-7102-8655","observed_orcids":["https://orcid.org/0000-0002-7102-8655"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Frank Soldner","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/nucleofection","display_name":"nucleofection","score":0.8690000176429749},{"id":"https://openalex.org/keywords/human-pluripotent-stem-cells","display_name":"human pluripotent stem cells","score":0.9829999804496765},{"id":"https://openalex.org/keywords/induced-pluripotent-stem-cells","display_name":"induced pluripotent stem cells","score":0.8700000047683716},{"id":"https://openalex.org/keywords/embryonic-stem-cells","display_name":"embryonic stem cells","score":0.9350000023841858},{"id":"https://openalex.org/keywords/plasmid-dna-delivery","display_name":"plasmid DNA delivery","score":0.902999997138977},{"id":"https://openalex.org/keywords/mrna-delivery","display_name":"mRNA delivery","score":0.953000009059906},{"id":"https://openalex.org/keywords/ribonucleoprotein-delivery","display_name":"ribonucleoprotein delivery","score":0.9660000205039978},{"id":"https://openalex.org/keywords/cell-preparation","display_name":"cell preparation","score":0.37400001287460327}],"concepts":[{"id":"https://openalex.org/C144251240","wikidata":"https://www.wikidata.org/wiki/Q7068229","display_name":"Nucleofection","level":4,"score":0.9767473340034485},{"id":"https://openalex.org/C107459253","wikidata":"https://www.wikidata.org/wiki/Q674925","display_name":"Induced pluripotent stem cell","level":4,"score":0.767778754234314},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.574316680431366},{"id":"https://openalex.org/C2780385302","wikidata":"https://www.wikidata.org/wiki/Q367158","display_name":"Protocol (science)","level":3,"score":0.5445986986160278},{"id":"https://openalex.org/C145103041","wikidata":"https://www.wikidata.org/wiki/Q1151519","display_name":"Embryonic stem cell","level":3,"score":0.4391297399997711},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.39929455518722534},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.361493319272995},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.31558725237846375},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.28960198163986206},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.1964692771434784},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.15189015865325928},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.09921121597290039},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.0},{"id":"https://openalex.org/C204787440","wikidata":"https://www.wikidata.org/wiki/Q188504","display_name":"Alternative medicine","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.17504/protocols.io.b4pgqvjw","doi":"https://doi.org/10.17504/protocols.io.b4pgqvjw","is_oa":true,"landing_page_url":"https://doi.org/10.17504/protocols.io.b4pgqvjw","pdf_url":null,"source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.17504/protocols.io.b4pgqvjw","doi":"https://doi.org/10.17504/protocols.io.b4pgqvjw","is_oa":true,"landing_page_url":"https://doi.org/10.17504/protocols.io.b4pgqvjw","pdf_url":null,"source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.504800021648407}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4315777449","https://openalex.org/W4211028474","https://openalex.org/W4211216669","https://openalex.org/W4315783129","https://openalex.org/W4211111883","https://openalex.org/W4211039258","https://openalex.org/W4211215737","https://openalex.org/W4211169758","https://openalex.org/W4211142980","https://openalex.org/W4210987414"],"abstract_inverted_index":{"This":[0,58],"protocol":[1,59,65,75,82],"describes":[2],"the":[3,14,30,74,77,91,126,144],"procedure":[4],"or":[5,19],"preparing":[6,115],"MEF-cultured":[7,85],"human":[8,105],"pluripotent":[9,106],"stem":[10,107],"cells":[11,63,103],"(hPSCs)":[12],"for":[13,53,64,104,114,120],"delivery":[15],"of":[16,67,96,133,147],"plasmids,":[17,116],"mRNA":[18],"ribonucleoprotein":[20],"(RNP)":[21],"using":[22],"nucleofection.":[23],"General":[24],"notes":[25],"1.":[26],"Throughout":[27],"this":[28,138,148],"protocol,":[29,149],"term":[31],"hPSC":[32],"is":[33,60],"used":[34],"to":[35,38,61,137],"collectively":[36],"refer":[37],"both":[39],"hiPSCs":[40,54],"and":[41,49,55,76,94,118,130],"hESCs.":[42,56],"All":[43],"described":[44],"procedures":[45],"have":[46],"been":[47],"tested":[48],"work":[50],"equally":[51],"well":[52],"2.":[57],"prepare":[62],"nucleofection":[66,121],"hPSCs.":[68],"Before":[69],"starting,":[70],"familiarize":[71],"yourself":[72],"with":[73],"required":[78],"preparations.":[79],"A":[80,135],"detailed":[81],"on":[83],"maintaining":[84],"hPSCs":[86],"can":[87,122,140],"be":[88,123,141],"found":[89,124,142],"in":[90,125,143],"collection":[92,127,139],"\"Maintenance":[93],"inactivation":[95],"mouse":[97],"embryonic":[98],"fibroblasts":[99],"(MEFs)":[100],"as":[101],"feeder":[102],"cell":[108],"culture;\"":[109],"doi:":[110],"3.":[111],"Detailed":[112],"protocols":[113],"RNA,":[117],"RNP":[119],"\"Nucleofection":[128],"(Amaxa)":[129],"electroporation":[131],"(Biorad)":[132],"hPSCs.\"":[134],"link":[136],"title":[145],"section":[146],"located":[150],"above":[151]},"counts_by_year":[],"updated_date":"2026-10-28T10:33:24.094632","created_date":"2022-02-13T00:00:00"}