{"id":"https://openalex.org/W4211159352","doi":"https://doi.org/10.17504/protocols.io.b4mnqu5e","title":"Thawing of hPSCs grown on MEFs v1","display_name":"Thawing of hPSCs grown on MEFs v1","publication_year":2022,"publication_date":"2022-02-03","ids":{"openalex":"https://openalex.org/W4211159352","doi":"https://doi.org/10.17504/protocols.io.b4mnqu5e"},"language":"en","primary_location":{"id":"doi:10.17504/protocols.io.b4mnqu5e","doi":"https://doi.org/10.17504/protocols.io.b4mnqu5e","is_oa":true,"landing_page_url":"https://doi.org/10.17504/protocols.io.b4mnqu5e","pdf_url":null,"source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://doi.org/10.17504/protocols.io.b4mnqu5e","any_repository_has_fulltext":null},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5038944590","display_name":"Hanqin Li","orcid":"https://orcid.org/0000-0001-7995-1084","observed_orcids":["https://orcid.org/0000-0001-7995-1084","https://orcid.org/0009-0008-3334-6899"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Hanqin Li","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0001-7995-1084","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5000495585","display_name":"Oriol Busquets","orcid":"https://orcid.org/0000-0002-1372-9699","observed_orcids":["https://orcid.org/0000-0002-1372-9699"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Oriol Busquets","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-1372-9699","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5082161278","display_name":"Steven Poser","orcid":"https://orcid.org/0000-0003-4868-1389","observed_orcids":["https://orcid.org/0000-0003-4868-1389"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Steven Poser","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0003-4868-1389","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5045835078","display_name":"Dirk F. Hockemeyer","orcid":"https://orcid.org/0000-0002-5598-5092","observed_orcids":["https://orcid.org/0000-0002-5598-5092"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Dirk Hockemeyer","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-5598-5092","affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5070152031","display_name":"Frank Soldner","orcid":"https://orcid.org/0000-0002-7102-8655","observed_orcids":["https://orcid.org/0000-0002-7102-8655"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Frank Soldner","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10176","display_name":"Mesenchymal stem cell research","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10268","display_name":"Developmental Biology and Gene Regulation","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/human-pluripotent-stem-cells","display_name":"human pluripotent stem cells","score":0.9700000286102295},{"id":"https://openalex.org/keywords/cell-thawing","display_name":"cell thawing","score":0.8460000157356262},{"id":"https://openalex.org/keywords/human-induced-pluripotent-stem-cells","display_name":"human induced pluripotent stem cells","score":0.8299999833106995},{"id":"https://openalex.org/keywords/human-embryonic-stem-cells","display_name":"human embryonic stem cells","score":0.9980000257492065},{"id":"https://openalex.org/keywords/cryopreservation","display_name":"cryopreservation","score":0.765999972820282},{"id":"https://openalex.org/keywords/low-oxygen-culture","display_name":"low oxygen culture","score":0.777999997138977},{"id":"https://openalex.org/keywords/cell-clusters","display_name":"cell clusters","score":0.5680000185966492},{"id":"https://openalex.org/keywords/cell-recovery","display_name":"cell recovery","score":0.5}],"concepts":[{"id":"https://openalex.org/C107459253","wikidata":"https://www.wikidata.org/wiki/Q674925","display_name":"Induced pluripotent stem cell","level":4,"score":0.8603082895278931},{"id":"https://openalex.org/C145103041","wikidata":"https://www.wikidata.org/wiki/Q1151519","display_name":"Embryonic stem cell","level":3,"score":0.7379869818687439},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5338578820228577},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.46655887365341187},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.41228288412094116},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.36558276414871216},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.11336946487426758},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.05120232701301575}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.17504/protocols.io.b4mnqu5e","doi":"https://doi.org/10.17504/protocols.io.b4mnqu5e","is_oa":true,"landing_page_url":"https://doi.org/10.17504/protocols.io.b4mnqu5e","pdf_url":null,"source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.17504/protocols.io.b4mnqu5e","doi":"https://doi.org/10.17504/protocols.io.b4mnqu5e","is_oa":true,"landing_page_url":"https://doi.org/10.17504/protocols.io.b4mnqu5e","pdf_url":null,"source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"},"sustainable_development_goals":[{"score":0.4000000059604645,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4211010059","https://openalex.org/W4211029808","https://openalex.org/W4211053417","https://openalex.org/W4211116910","https://openalex.org/W4211167858","https://openalex.org/W4410632977","https://openalex.org/W4211043233","https://openalex.org/W4410632870","https://openalex.org/W4211151394","https://openalex.org/W2991697637"],"abstract_inverted_index":{"This":[0],"protocol":[1],"describes":[2],"the":[3,25],"standard":[4],"procedure":[5],"of":[6,111],"thawing":[7],"human":[8],"pluripotent":[9],"stem":[10],"cells":[11],"(hPSCs)":[12],"on":[13],"inactivated":[14],"mouse":[15],"embryonic":[16],"fibroblasts":[17],"(MEFs).":[18],"General":[19],"notes":[20],"1.":[21],"Throughout":[22],"this":[23],"protocol,":[24],"term":[26],"hPSC":[27],"is":[28],"used":[29,118],"to":[30,33],"collectively":[31],"refer":[32],"both":[34,119],"hiPSCs":[35,49],"and":[36,44,50,121],"hESCs.":[37,51],"All":[38],"described":[39],"procedures":[40],"have":[41,71,117],"been":[42],"tested":[43],"work":[45],"equally":[46],"well":[47],"for":[48],"2.":[52],"Until":[53],"otherwise":[54],"indicated,":[55],"hPSCs":[56,74,94,112],"are":[57],"routinely":[58],"grown":[59],"in":[60,103],"a":[61],"humidified":[62],"cell":[63,97,105,114],"culture":[64],"incubator":[65],"under":[66],"\u201clow\u201d":[67],"oxygen":[68],"conditions.":[69,90],"We":[70,116],"successfully":[72],"maintained":[73],"using":[75],"either":[76],"3%":[77],"O2":[78,85],"(3%":[79],"O2,":[80,87],"5%":[81,84,88],"CO2)":[82,89],"or":[83],"(5%":[86],"3.":[91],"While":[92],"freezing":[93,110],"as":[95,113],"single":[96],"solution":[98],"(using":[99],"Rock":[100],"Inhibitor)":[101],"results":[102],"better":[104],"recovery,":[106],"some":[107],"laboratories":[108],"prefer":[109],"clusters.":[115],"approaches":[120],"do":[122],"not":[123],"observe":[124],"obvious":[125],"differences.":[126]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2022-02-13T00:00:00"}