{"id":"https://openalex.org/W4206405015","doi":"https://doi.org/10.4236/ojgen.2021.114010","title":"A Universal Real-Time Fluorescence qPCR Method for Identifying Epidemic Strains of African Swine Fever Virus","display_name":"A Universal Real-Time Fluorescence qPCR Method for Identifying Epidemic Strains of African Swine Fever Virus","publication_year":2021,"publication_date":"2021-01-01","ids":{"openalex":"https://openalex.org/W4206405015","doi":"https://doi.org/10.4236/ojgen.2021.114010"},"language":"en","primary_location":{"id":"doi:10.4236/ojgen.2021.114010","doi":"https://doi.org/10.4236/ojgen.2021.114010","is_oa":true,"landing_page_url":"https://doi.org/10.4236/ojgen.2021.114010","pdf_url":"https://content.scirp.org/pdf/ojgen_2021121515393824.pdf","source":{"id":"https://openalex.org/S2765021627","display_name":"Open Journal of Genetics","issn_l":"2162-4453","issn":["2162-4453","2162-4461"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310320480","host_organization_name":"Scientific Research Publishing","host_organization_lineage":["https://openalex.org/P4310320480"],"host_organization_lineage_names":["Scientific Research Publishing"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Open Journal of Genetics","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"https://content.scirp.org/pdf/ojgen_2021121515393824.pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5074998434","display_name":"Meihui Lv","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I61565387","display_name":"Dalian Minzu University","ror":"https://ror.org/02hxfx521","country_code":"CN","type":"education","lineage":["https://openalex.org/I61565387"]},{"id":"https://openalex.org/I88372448","display_name":"Dalian Polytechnic University","ror":"https://ror.org/00c7x4a95","country_code":"CN","type":"education","lineage":["https://openalex.org/I88372448"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Meihui Lv","raw_affiliation_strings":["Dalian Polytechnic University, Dalian, China","Key Laboratory of Biotechnology and Bioresources Utilization of Ministry of Education, Dalian Minzu University, Dalian, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Dalian Polytechnic University, Dalian, China","institution_ids":["https://openalex.org/I88372448"]},{"raw_affiliation_string":"Key Laboratory of Biotechnology and Bioresources Utilization of Ministry of Education, Dalian Minzu University, Dalian, China","institution_ids":["https://openalex.org/I61565387"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102361113","display_name":"Qiuyue Zheng","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I61565387","display_name":"Dalian Minzu University","ror":"https://ror.org/02hxfx521","country_code":"CN","type":"education","lineage":["https://openalex.org/I61565387"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Qiuyue Zheng","raw_affiliation_strings":["Key Laboratory of Biotechnology and Bioresources Utilization of Ministry of Education, Dalian Minzu University, Dalian, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Key Laboratory of Biotechnology and Bioresources Utilization of Ministry of Education, Dalian Minzu University, Dalian, China","institution_ids":["https://openalex.org/I61565387"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5019260570","display_name":"Lili Yang","orcid":"https://orcid.org/0000-0001-6568-0404","observed_orcids":["https://orcid.org/0000-0001-6568-0404"]},"institutions":[{"id":"https://openalex.org/I61565387","display_name":"Dalian Minzu University","ror":"https://ror.org/02hxfx521","country_code":"CN","type":"education","lineage":["https://openalex.org/I61565387"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Lili Yang","raw_affiliation_strings":["Key Laboratory of Biotechnology and Bioresources Utilization of Ministry of Education, Dalian Minzu University, Dalian, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Key Laboratory of Biotechnology and Bioresources Utilization of Ministry of Education, Dalian Minzu University, Dalian, China","institution_ids":["https://openalex.org/I61565387"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100403191","display_name":"Lin Wang","orcid":"https://orcid.org/0000-0002-7215-8750","observed_orcids":["https://orcid.org/0000-0002-7215-8750","https://orcid.org/0009-0008-7915-5705"]},"institutions":[{"id":"https://openalex.org/I4210092847","display_name":"China Animal Disease Control Center","ror":"https://ror.org/00gwv7d20","country_code":"CN","type":"government","lineage":["https://openalex.org/I4210092847"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Lin Wang","raw_affiliation_strings":["Beijing Animal Disease Prevention and Control Center, Beijing, China","Tianjin , China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Beijing Animal Disease Prevention and Control Center, Beijing, China","institution_ids":["https://openalex.org/I4210092847"]},{"raw_affiliation_string":"Tianjin , China","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100349899","display_name":"Li-Li Chen","orcid":"https://orcid.org/0000-0002-9593-8004","observed_orcids":["https://orcid.org/0000-0002-9593-8004"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Lili Chen","raw_affiliation_strings":["Zhonglianrui (Beijing) Biotechnology Co., Ltd., Beijing, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Zhonglianrui (Beijing) Biotechnology Co., Ltd., Beijing, China","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108776942","display_name":"Aifu Yang","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Aifu Yang","raw_affiliation_strings":["Dalian Customs Technology Center, Dalian, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Dalian Customs Technology Center, Dalian, China","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5101081579","display_name":"Jijuan Cao","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I61565387","display_name":"Dalian Minzu University","ror":"https://ror.org/02hxfx521","country_code":"CN","type":"education","lineage":["https://openalex.org/I61565387"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Jijuan Cao","raw_affiliation_strings":["Key Laboratory of Biotechnology and Bioresources Utilization of Ministry of Education, Dalian Minzu University, Dalian, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Key Laboratory of Biotechnology and Bioresources Utilization of Ministry of Education, Dalian Minzu University, Dalian, China","institution_ids":["https://openalex.org/I61565387"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5101081579"],"corresponding_institution_ids":["https://openalex.org/I61565387"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.28340657,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"11","issue":"04","first_page":"102","last_page":"119"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11560","display_name":"Animal Disease Management and Epidemiology","score":0.9965999722480774,"subfield":{"id":"https://openalex.org/subfields/1102","display_name":"Agronomy and Crop Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11560","display_name":"Animal Disease Management and Epidemiology","score":0.9965999722480774,"subfield":{"id":"https://openalex.org/subfields/1102","display_name":"Agronomy and Crop Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.0007999999797903001,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11495","display_name":"Animal Virus Infections Studies","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/african-swine-fever-virus","display_name":"African swine fever virus","score":1.0},{"id":"https://openalex.org/keywords/p72-gene","display_name":"p72 gene","score":0.8809999823570251},{"id":"https://openalex.org/keywords/primer-and-probe-design","display_name":"primer and probe design","score":0.6909999847412109},{"id":"https://openalex.org/keywords/dimethyl-sulfoxide","display_name":"dimethyl sulfoxide","score":0.8190000057220459},{"id":"https://openalex.org/keywords/detection-limit","display_name":"detection limit","score":0.968999981880188},{"id":"https://openalex.org/keywords/early-diagnosis","display_name":"early diagnosis","score":0.7429999709129333},{"id":"https://openalex.org/keywords/false-negative-reduction","display_name":"false negative reduction","score":0.4740000069141388}],"concepts":[{"id":"https://openalex.org/C2778359420","wikidata":"https://www.wikidata.org/wiki/Q195760","display_name":"African swine fever virus","level":3,"score":0.8082211017608643},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.7593278884887695},{"id":"https://openalex.org/C2777563447","wikidata":"https://www.wikidata.org/wiki/Q7243397","display_name":"Primer (cosmetics)","level":2,"score":0.702813982963562},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6565253734588623},{"id":"https://openalex.org/C48023723","wikidata":"https://www.wikidata.org/wiki/Q856198","display_name":"Real-time polymerase chain reaction","level":3,"score":0.5918266773223877},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.5323385000228882},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.4893617033958435},{"id":"https://openalex.org/C135763542","wikidata":"https://www.wikidata.org/wiki/Q106016","display_name":"Genotype","level":3,"score":0.4696962833404541},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.2787446975708008},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.08468973636627197},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.0582541823387146},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.4236/ojgen.2021.114010","doi":"https://doi.org/10.4236/ojgen.2021.114010","is_oa":true,"landing_page_url":"https://doi.org/10.4236/ojgen.2021.114010","pdf_url":"https://content.scirp.org/pdf/ojgen_2021121515393824.pdf","source":{"id":"https://openalex.org/S2765021627","display_name":"Open Journal of Genetics","issn_l":"2162-4453","issn":["2162-4453","2162-4461"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310320480","host_organization_name":"Scientific Research Publishing","host_organization_lineage":["https://openalex.org/P4310320480"],"host_organization_lineage_names":["Scientific Research Publishing"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Open Journal of Genetics","raw_type":"journal-article"}],"best_oa_location":{"id":"doi:10.4236/ojgen.2021.114010","doi":"https://doi.org/10.4236/ojgen.2021.114010","is_oa":true,"landing_page_url":"https://doi.org/10.4236/ojgen.2021.114010","pdf_url":"https://content.scirp.org/pdf/ojgen_2021121515393824.pdf","source":{"id":"https://openalex.org/S2765021627","display_name":"Open Journal of Genetics","issn_l":"2162-4453","issn":["2162-4453","2162-4461"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310320480","host_organization_name":"Scientific Research Publishing","host_organization_lineage":["https://openalex.org/P4310320480"],"host_organization_lineage_names":["Scientific Research Publishing"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Open Journal of Genetics","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.8048999905586243,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"},{"score":0.40450000762939453,"display_name":"Zero hunger","id":"https://metadata.un.org/sdg/2"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4206405015.pdf","grobid_xml":"https://content.openalex.org/works/W4206405015.grobid-xml"},"referenced_works_count":26,"referenced_works":["https://openalex.org/W1988329753","https://openalex.org/W2011602486","https://openalex.org/W2057510110","https://openalex.org/W2069297400","https://openalex.org/W2083506607","https://openalex.org/W2186150742","https://openalex.org/W2258909125","https://openalex.org/W2527730660","https://openalex.org/W2613902351","https://openalex.org/W2800195230","https://openalex.org/W2887875179","https://openalex.org/W2955469799","https://openalex.org/W2972351935","https://openalex.org/W2974136274","https://openalex.org/W2984362742","https://openalex.org/W3002659938","https://openalex.org/W3010975403","https://openalex.org/W3017302946","https://openalex.org/W3037555113","https://openalex.org/W3040677640","https://openalex.org/W3043866022","https://openalex.org/W3084745381","https://openalex.org/W3133808080","https://openalex.org/W3134102336","https://openalex.org/W3176574200","https://openalex.org/W3184971779"],"related_works":["https://openalex.org/W2971708102","https://openalex.org/W4384299231","https://openalex.org/W2137618288","https://openalex.org/W2390410004","https://openalex.org/W2367545400","https://openalex.org/W4210565841","https://openalex.org/W2172600235","https://openalex.org/W2348791923","https://openalex.org/W4285189994","https://openalex.org/W4296838633"],"abstract_inverted_index":{"Objective":[0],"Establishing":[1],"a":[2],"highly":[3],"sensitive":[4],"real-time":[5],"fluorescence":[6],"quantitative":[7],"PCR":[8],"(qPCR)":[9],"method":[10,98,133,150,171],"for":[11,43,81,124,129,143,153,175],"universal":[12,176],"testing":[13,122,154,177],"of":[14,40,52,107,178,186],"epidemic":[15,163,179],"African":[16],"swine":[17],"fever":[18],"virus":[19,160],"(ASFV)":[20],"strains.":[21],"Methods":[22],"The":[23,37,69,97],"ASFV":[24,125,180,189],"p72":[25,35],"gene":[26],"was":[27,46,79,99,126],"targeted":[28],"to":[29,101],"design":[30],"primer":[31],"probes":[32],"covering":[33],"24":[34],"genotypes.":[36],"optimal":[38,70,80],"amount":[39],"dimethylsulphoxide":[41],"(DMSO)":[42],"qPCR":[44,132],"amplification":[45],"determined,":[47],"Various":[48],"sensitivity":[49],"and":[50,58,63,83,112,142,162,188,195],"limit":[51],"detection":[53],"(LOD)":[54],"tests":[55],"were":[56,66,117],"performed,":[57],"clinical":[59],"samples":[60,116,157],"from":[61],"China":[62],"imported":[64,167],"goods":[65],"tested.":[67],"Results":[68],"primer-probe":[71],"combination":[72],"could":[73,172],"specifically":[74],"detect":[75],"ASFV,":[76],"1.5%":[77],"DMSO":[78],"qPCR,":[82],"LOD":[84],"reached":[85],"3.2":[86],"copies/\u03bcL":[87],"with":[88,158],"good":[89],"reproducibility":[90],"(n":[91],"=":[92,95],"20,":[93],"p":[94],"0.369).":[96],"employed":[100,174],"test":[102],"142":[103],"clinically":[104],"suspected":[105],"samples,":[106],"which":[108],"30":[109],"pig":[110,114],"blood":[111],"37":[113],"tissue":[115],"ASFV-positive.":[118],"Moreover,":[119],"the":[120,130,136,144],"positive":[121,156],"rate":[123],"higher":[127],"than":[128],"standard":[131],"recommended":[134],"by":[135],"Office":[137],"International":[138],"Des":[139],"Epizooties":[140],"(OIE),":[141],"commercially":[145],"available":[146],"kit.":[147],"Thus,":[148],"our":[149],"is":[151],"superior":[152],"weakly":[155],"low":[159],"titre,":[161],"strains":[164,181],"present":[165],"in":[166],"goods.":[168],"Conclusion":[169],"Our":[170],"be":[173],"worldwide,":[182],"ensuring":[183],"wider":[184],"coverage":[185],"hosts":[187],"strains/endemic":[190],"strains,":[191],"reducing":[192],"false":[193],"negatives,":[194],"benefitting":[196],"early":[197],"diagnosis.":[198]},"counts_by_year":[],"updated_date":"2026-10-30T12:39:25.321706","created_date":"2022-01-25T00:00:00"}
