{"id":"https://openalex.org/W4206329675","doi":"https://doi.org/10.1101/pdb.prot5062","title":"Construction of Nucleic Acid Programmable Protein Arrays (NAPPA) 7: Detecting DNA on NAPPA Slides: Figure 1.","display_name":"Construction of Nucleic Acid Programmable Protein Arrays (NAPPA) 7: Detecting DNA on NAPPA Slides: Figure 1.","publication_year":2008,"publication_date":"2008-11-01","ids":{"openalex":"https://openalex.org/W4206329675","doi":"https://doi.org/10.1101/pdb.prot5062"},"language":"en","primary_location":{"id":"doi:10.1101/pdb.prot5062","doi":"https://doi.org/10.1101/pdb.prot5062","is_oa":false,"landing_page_url":"https://doi.org/10.1101/pdb.prot5062","pdf_url":null,"source":{"id":"https://openalex.org/S119953358","display_name":"Cold Spring Harbor Protocols","issn_l":"1559-6095","issn":["1559-6095","1940-3402"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315909","host_organization_name":"Cold Spring Harbor Laboratory Press","host_organization_lineage":["https://openalex.org/P4310315909"],"host_organization_lineage_names":["Cold Spring Harbor Laboratory Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cold Spring Harbor Protocols","raw_type":"journal-article"},"volume":null,"type":"preprint","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5055555857","display_name":"Andrew J. Link","orcid":"https://orcid.org/0000-0003-0885-5786","observed_orcids":["https://orcid.org/0000-0003-0885-5786"]},"institutions":[{"id":"https://openalex.org/I200719446","display_name":"Vanderbilt University","ror":"https://ror.org/02vm5rt34","country_code":"US","type":"education","lineage":["https://openalex.org/I200719446"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Andrew J. Link","raw_affiliation_strings":["Vanderbilt University School of Medicine, Nashville, TN 37232-8575, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Vanderbilt University School of Medicine, Nashville, TN 37232-8575, USA","institution_ids":["https://openalex.org/I200719446"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5046993686","display_name":"Joshua LaBaer","orcid":"https://orcid.org/0000-0001-5788-9697","observed_orcids":["https://orcid.org/0000-0001-5788-9697"]},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Joshua LaBaer","raw_affiliation_strings":["Harvard University School of Medicine, Harvard Institute of Proteomics, Cambridge, MA 02141-2023, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Harvard University School of Medicine, Harvard Institute of Proteomics, Cambridge, MA 02141-2023, USA","institution_ids":["https://openalex.org/I136199984"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5046993686"],"corresponding_institution_ids":["https://openalex.org/I136199984"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":9,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"2008","issue":"11","first_page":"pdb.prot5062","last_page":"pdb.prot5062"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12867","display_name":"Advanced Biosensing Techniques and Applications","score":0.9940999746322632,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12867","display_name":"Advanced Biosensing Techniques and Applications","score":0.9940999746322632,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10885","display_name":"Gene expression and cancer classification","score":0.0010999999940395355,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.0006000000284984708,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/nucleic-acid-programmable-protein-arrays","display_name":"nucleic acid programmable protein arrays","score":0.9340000152587891},{"id":"https://openalex.org/keywords/dna-detection","display_name":"DNA detection","score":0.5899999737739563},{"id":"https://openalex.org/keywords/in-vitro-translation","display_name":"in vitro translation","score":0.6660000085830688},{"id":"https://openalex.org/keywords/protein-microarrays","display_name":"protein microarrays","score":0.7639999985694885},{"id":"https://openalex.org/keywords/dna-capture","display_name":"DNA capture","score":0.5},{"id":"https://openalex.org/keywords/functional-proteomics","display_name":"functional proteomics","score":0.7059999704360962}],"concepts":[{"id":"https://openalex.org/C95371953","wikidata":"https://www.wikidata.org/wiki/Q591745","display_name":"DNA microarray","level":4,"score":0.7834424376487732},{"id":"https://openalex.org/C195814341","wikidata":"https://www.wikidata.org/wiki/Q494918","display_name":"Protein microarray","level":5,"score":0.773353099822998},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.6058053970336914},{"id":"https://openalex.org/C187882448","wikidata":"https://www.wikidata.org/wiki/Q283478","display_name":"Complementary DNA","level":3,"score":0.5930809378623962},{"id":"https://openalex.org/C24107716","wikidata":"https://www.wikidata.org/wiki/Q123619","display_name":"Nucleic acid","level":2,"score":0.5552524924278259},{"id":"https://openalex.org/C46111723","wikidata":"https://www.wikidata.org/wiki/Q471857","display_name":"Proteomics","level":3,"score":0.47229912877082825},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.4216092824935913},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.413136750459671},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3735816478729248},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.37136369943618774},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.36194008588790894},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.24197116494178772},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.21610814332962036}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1101/pdb.prot5062","doi":"https://doi.org/10.1101/pdb.prot5062","is_oa":false,"landing_page_url":"https://doi.org/10.1101/pdb.prot5062","pdf_url":null,"source":{"id":"https://openalex.org/S119953358","display_name":"Cold Spring Harbor Protocols","issn_l":"1559-6095","issn":["1559-6095","1940-3402"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315909","host_organization_name":"Cold Spring Harbor Laboratory Press","host_organization_lineage":["https://openalex.org/P4310315909"],"host_organization_lineage_names":["Cold Spring Harbor Laboratory Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cold Spring Harbor Protocols","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":6,"referenced_works":["https://openalex.org/W1965478431","https://openalex.org/W2004089475","https://openalex.org/W2013820117","https://openalex.org/W2087647461","https://openalex.org/W2116432699","https://openalex.org/W4235510142"],"related_works":["https://openalex.org/W4235510142","https://openalex.org/W2013820117","https://openalex.org/W1965478431","https://openalex.org/W2004089475","https://openalex.org/W2116432699","https://openalex.org/W2087647461","https://openalex.org/W1572553812","https://openalex.org/W4242177277","https://openalex.org/W120526023","https://openalex.org/W2099839475"],"abstract_inverted_index":{"INTRODUCTION":[0],"Functional":[1],"proteomics":[2],"enables":[3],"protein":[4,69],"activities":[5],"to":[6,34,42,94,156,181,218],"be":[7,168,179,187],"studied":[8],"in":[9,51,98,120,170],"vitro":[10],"using":[11,205],"high-throughput":[12],"(HT)":[13],"methods.":[14],"Protein":[15,37],"microarrays":[16,38,70],"are":[17,39,71,117,154,196],"the":[18,45,57,66,79,96,101,129,141,143,147,157,220],"method":[19,105],"of":[20,47,61,126,134,140,161,201,213,222],"choice":[21],"because":[22],"they":[23],"display":[24],"many":[25,48,62],"proteins":[26,63,80,97,115,136,153,202],"simultaneously":[27],"and":[28,77,111,164,172,203,207],"require":[29],"only":[30],"small":[31],"reaction":[32],"volumes":[33],"assess":[35,219],"function.":[36],"typically":[40],"used":[41],"(1)":[43],"measure":[44],"abundance":[46],"different":[49],"analytes":[50],"a":[52,82],"sample":[53],"or":[54,59],"(2)":[55],"study":[56],"functions":[58],"properties":[60],"spotted":[64],"on":[65,100,215],"array.":[67,158],"Target":[68],"usually":[72],"generated":[73],"by":[74,199],"expressing,":[75],"purifying,":[76],"spotting":[78],"onto":[81],"solid":[83],"surface":[84],"at":[85,137],"very":[86],"close":[87],"spatial":[88],"density.":[89],"An":[90],"alternative":[91],"approach":[92],"is":[93],"translate":[95,112],"situ":[99],"array":[102],"surface.":[103],"This":[104,209],"uses":[106],"cell-free":[107],"extracts":[108],"that":[109,150],"transcribe":[110],"DNA":[113,165,176,204,214,223],"into":[114,128],"which":[116,185],"then":[118,178],"captured":[119],"situ,":[121],"thus":[122],"converting":[123],"cDNA":[124,144],"copies":[125],"genes":[127,149],"desired":[130,152],"target":[131],"proteins.":[132],"Instead":[133],"printing":[135],"each":[138],"feature":[139],"array,":[142],"molecules":[145],"for":[146,190],"corresponding":[148],"produce":[151],"affixed":[155],"Chemical":[159],"treatment":[160],"glass":[162],"slides":[163,195,217],"isolation":[166],"can":[167,177,186],"performed":[169],"advance":[171],"stored.":[173],"The":[174],"plasmid":[175],"printed":[180],"make":[182],"NAPPA":[183,194],"slides,":[184],"stored":[188],"dry":[189],"use.":[191],"For":[192],"experiments,":[193],"expressed":[197],"followed":[198],"detection":[200,212],"antibodies":[206],"stains.":[208],"protocol":[210],"describes":[211],"arrayed":[216],"amount":[221],"captured.":[224]},"counts_by_year":[],"updated_date":"2026-07-31T08:31:51.225901","created_date":"2022-01-25T00:00:00"}