{"id":"https://openalex.org/W4205202085","doi":"https://doi.org/10.3791/52381-v","title":"Modeling The Lifecycle Of Ebola Virus Under Biosafety Level 2 Conditions With Virus-like Particles Containing Tetracistronic Minigenomes","display_name":"Modeling The Lifecycle Of Ebola Virus Under Biosafety Level 2 Conditions With Virus-like Particles Containing Tetracistronic Minigenomes","publication_year":2014,"publication_date":"2014-09-27","ids":{"openalex":"https://openalex.org/W4205202085","doi":"https://doi.org/10.3791/52381-v"},"language":"en","primary_location":{"id":"doi:10.3791/52381-v","doi":"https://doi.org/10.3791/52381-v","is_oa":true,"landing_page_url":"https://doi.org/10.3791/52381-v","pdf_url":"https://www.jove.com/pdf/52381/modeling-lifecycle-ebola-virus-under-biosafety-level-2-conditions","source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://www.jove.com/pdf/52381/modeling-lifecycle-ebola-virus-under-biosafety-level-2-conditions","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5013946002","display_name":"Thomas Hoenen","orcid":"https://orcid.org/0000-0002-5829-6305","observed_orcids":["https://orcid.org/0000-0002-5829-6305"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210134534","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Thomas Hoenen","raw_affiliation_strings":["Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5025203891","display_name":"Ari Watt","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210134534","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ari Watt","raw_affiliation_strings":["Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5104022376","display_name":"Anita Mora","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210134534","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Anita Mora","raw_affiliation_strings":["Research Technology Branch, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Research Technology Branch, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5051760718","display_name":"Heinz Feldmann","orcid":"https://orcid.org/0000-0001-9448-8227","observed_orcids":["https://orcid.org/0000-0001-9448-8227"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210134534","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Heinz Feldmann","raw_affiliation_strings":["Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210134534"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":1.1762,"has_fulltext":true,"cited_by_count":12,"citation_normalized_percentile":{"value":0.80745463,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":null,"issue":"91","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11581","display_name":"Viral Infections and Outbreaks Research","score":0.9907000064849854,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11581","display_name":"Viral Infections and Outbreaks Research","score":0.9907000064849854,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11135","display_name":"Virology and Viral Diseases","score":0.004699999932199717,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12047","display_name":"Viral Infections and Vectors","score":0.002199999988079071,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/ebola-virus","display_name":"Ebola virus","score":0.9990000128746033},{"id":"https://openalex.org/keywords/virus-like-particles","display_name":"virus-like particles","score":0.8349999785423279},{"id":"https://openalex.org/keywords/biosafety-level-2","display_name":"biosafety level 2","score":0.9240000247955322},{"id":"https://openalex.org/keywords/vp40","display_name":"VP40","score":0.9419999718666077},{"id":"https://openalex.org/keywords/vp24","display_name":"VP24","score":1.0},{"id":"https://openalex.org/keywords/viral-morphogenesis","display_name":"viral morphogenesis","score":0.847000002861023},{"id":"https://openalex.org/keywords/hemorrhagic-fever","display_name":"hemorrhagic fever","score":0.6430000066757202},{"id":"https://openalex.org/keywords/reporter-genes","display_name":"reporter genes","score":0.4819999933242798}],"concepts":[{"id":"https://openalex.org/C2777469322","wikidata":"https://www.wikidata.org/wiki/Q8064876","display_name":"Ebola virus","level":3,"score":0.9214890003204346},{"id":"https://openalex.org/C86295872","wikidata":"https://www.wikidata.org/wiki/Q24779573","display_name":"VP40","level":4,"score":0.826860785484314},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.6972509026527405},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6450448036193848},{"id":"https://openalex.org/C52384281","wikidata":"https://www.wikidata.org/wiki/Q864713","display_name":"Biosafety","level":2,"score":0.599311351776123},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.5473182797431946},{"id":"https://openalex.org/C2909623084","wikidata":"https://www.wikidata.org/wiki/Q5331908","display_name":"Ebolavirus","level":4,"score":0.5395123362541199},{"id":"https://openalex.org/C140704245","wikidata":"https://www.wikidata.org/wiki/Q3933202","display_name":"Viral replication","level":3,"score":0.471633642911911},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.4139411747455597},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3207437992095947},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3140316605567932},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.22596874833106995},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.09058341383934021}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.3791/52381-v","doi":"https://doi.org/10.3791/52381-v","is_oa":true,"landing_page_url":"https://doi.org/10.3791/52381-v","pdf_url":"https://www.jove.com/pdf/52381/modeling-lifecycle-ebola-virus-under-biosafety-level-2-conditions","source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"}],"best_oa_location":{"id":"doi:10.3791/52381-v","doi":"https://doi.org/10.3791/52381-v","is_oa":true,"landing_page_url":"https://doi.org/10.3791/52381-v","pdf_url":"https://www.jove.com/pdf/52381/modeling-lifecycle-ebola-virus-under-biosafety-level-2-conditions","source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.8048999905586243,"id":"https://metadata.un.org/sdg/3"}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320309123","display_name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4205202085.pdf","grobid_xml":"https://content.openalex.org/works/W4205202085.grobid-xml"},"referenced_works_count":15,"referenced_works":["https://openalex.org/W1973590086","https://openalex.org/W1985403315","https://openalex.org/W2018581823","https://openalex.org/W2029424710","https://openalex.org/W2031407639","https://openalex.org/W2058238987","https://openalex.org/W2073541969","https://openalex.org/W2095606194","https://openalex.org/W2096009548","https://openalex.org/W2112449136","https://openalex.org/W2120572579","https://openalex.org/W2126089986","https://openalex.org/W2144862305","https://openalex.org/W2164859440","https://openalex.org/W2171288645"],"related_works":["https://openalex.org/W2620796237","https://openalex.org/W2029424710","https://openalex.org/W2884821517","https://openalex.org/W4308414513","https://openalex.org/W2095606194","https://openalex.org/W2740536161","https://openalex.org/W2888415989","https://openalex.org/W7213628065","https://openalex.org/W4280618545","https://openalex.org/W2620577340"],"abstract_inverted_index":{"Ebola":[0,38,92,101,136,169,242,255],"viruses":[1,39,175,256],"cause":[2],"severe":[3],"hemorrhagic":[4],"fevers":[5],"in":[6,105,182,195,250,257],"humans":[7],"and":[8,34,99,108,112,120,143,171,187,190,206,219,244],"non-human":[9],"primates,":[10],"with":[11,36,225],"case":[12,181],"fatality":[13],"rates":[14],"as":[15,17,216,270,272],"high":[16],"90%.":[18],"There":[19],"are":[20,165,192],"no":[21],"approved":[22],"vaccines":[23],"or":[24,82],"specific":[25],"treatments":[26],"for":[27,140,178,202,241,248],"the":[28,49,58,79,135,159,180,212,229,234,252,265],"disease":[29],"caused":[30],"by":[31],"these":[32,52,126],"viruses,":[33,243],"work":[35],"infectious":[37,85,151],"is":[40],"restricted":[41],"to":[42,74,117,147],"biosafety":[43,62,154],"level":[44,63,155],"4":[45],"laboratories,":[46],"significantly":[47],"limiting":[48],"research":[50],"on":[51,264,273],"viruses.":[53],"Lifecycle":[54],"modeling":[55,238],"systems":[56,70,164,183],"model":[57,149],"virus":[59,80,93,102,137,170,213],"lifecycle":[60,214,237],"under":[61,153],"2":[64,156],"conditions;":[65],"however,":[66],"until":[67],"recently":[68],"such":[69,215],"have":[71],"been":[72],"limited":[73],"either":[75],"individual":[76],"aspects":[77,210],"of":[78,91,162,211,254,267],"lifecycle,":[81],"a":[83,96],"single":[84],"cycle.":[86],"Tetracistronic":[87],"minigenomes,":[88],"which":[89],"consist":[90],"non-coding":[94],"regions,":[95],"reporter":[97],"gene,":[98],"three":[100],"genes":[103],"involved":[104],"morphogenesis,":[106,204],"budding,":[107],"entry":[109],"(VP40,":[110],"GP1,2,":[111],"VP24),":[113],"can":[114,130,221],"be":[115,223],"used":[116],"produce":[118],"replication":[119,142,218],"transcription-competent":[121],"virus-like":[122],"particles":[123],"(trVLPs)":[124],"containing":[125],"minigenomes.":[127],"These":[128],"trVLPs":[129],"continuously":[131],"infect":[132],"cells":[133],"expressing":[134],"proteins":[138],"responsible":[139],"genome":[141,217],"transcription,":[144],"allowing":[145],"us":[146],"safely":[148],"multiple":[150],"cycles":[152],"conditions.":[157],"Importantly,":[158],"viral":[160],"components":[161],"this":[163,196,226,268],"solely":[166],"derived":[167],"from":[168,173],"not":[172,193],"other":[174,209],"(as":[176],"is,":[177],"example,":[179],"using":[184],"pseudotyped":[185],"viruses),":[186],"VP40,":[188],"GP1,2":[189],"VP24":[191],"overexpressed":[194],"system,":[197,269],"making":[198],"it":[199],"ideally":[200],"suited":[201],"studying":[203],"budding":[205],"entry,":[207],"although":[208],"transcription":[220],"also":[222],"modeled":[224],"system.":[227],"Therefore,":[228],"tetracistronic":[230],"trVLP":[231],"assay":[232],"represents":[233],"most":[235],"comprehensive":[236],"system":[239],"available":[240],"has":[245],"tremendous":[246],"potential":[247],"use":[249,266],"investigating":[251],"biology":[253],"future.":[258],"Here,":[259],"we":[260],"provide":[261],"detailed":[262],"information":[263],"well":[271],"expected":[274],"results.":[275]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2021,"cited_by_count":3},{"year":2019,"cited_by_count":2},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":1}],"updated_date":"2026-10-08T09:06:37.294493","created_date":"2022-01-26T00:00:00"}
