{"id":"https://openalex.org/W4200549490","doi":"https://doi.org/10.1016/j.chembiol.2021.12.001","title":"Making the cut with protease engineering","display_name":"Making the cut with protease engineering","publication_year":2021,"publication_date":"2021-12-17","ids":{"openalex":"https://openalex.org/W4200549490","doi":"https://doi.org/10.1016/j.chembiol.2021.12.001","pmid":"https://pubmed.ncbi.nlm.nih.gov/34921772","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/9127713"},"language":"en","primary_location":{"id":"doi:10.1016/j.chembiol.2021.12.001","doi":"https://doi.org/10.1016/j.chembiol.2021.12.001","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.chembiol.2021.12.001","pdf_url":"http://www.cell.com/article/S2451945621005158/pdf","source":{"id":"https://openalex.org/S4210231961","display_name":"Cell chemical biology","issn_l":"2451-9448","issn":["2451-9448","2451-9456"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cell Chemical Biology","raw_type":"journal-article"},"volume":null,"type":"review","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"http://www.cell.com/article/S2451945621005158/pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5020267591","display_name":"Rebekah P. Dyer","orcid":"https://orcid.org/0000-0001-5407-3561","observed_orcids":["https://orcid.org/0000-0001-5407-3561"]},"institutions":[{"id":"https://openalex.org/I204250578","display_name":"University of California, Irvine","ror":"https://ror.org/04gyf1771","country_code":"US","type":"education","lineage":["https://openalex.org/I204250578"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Rebekah P. Dyer","raw_affiliation_strings":["Department of Molecular Biology and Biochemistry, University of California, Irvine, 1102 NS-2, Irvine, CA 92697-2025, USA"],"raw_orcid":"https://orcid.org/0000-0001-5407-3561","affiliations":[{"raw_affiliation_string":"Department of Molecular Biology and Biochemistry, University of California, Irvine, 1102 NS-2, Irvine, CA 92697-2025, USA","institution_ids":["https://openalex.org/I204250578"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5006888208","display_name":"Gregory A. Weiss","orcid":"https://orcid.org/0000-0003-0296-9846","observed_orcids":["https://orcid.org/0000-0003-0296-9846"]},"institutions":[{"id":"https://openalex.org/I204250578","display_name":"University of California, Irvine","ror":"https://ror.org/04gyf1771","country_code":"US","type":"education","lineage":["https://openalex.org/I204250578"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Gregory A. Weiss","raw_affiliation_strings":["Department of Chemistry, University of California, Irvine, 1102 NS-2, Irvine, CA 92697-2025, USA; Department of Molecular Biology and Biochemistry, University of California, Irvine, 1102 NS-2, Irvine, CA 92697-2025, USA; Department of Pharmaceutical Sciences, University of California, Irvine, 1102 NS-2, Irvine, CA 92697-2025, USA. Electronic address: gweiss@uci.edu","Department of Chemistry, University of California, Irvine, 1102 NS-2, Irvine, CA 92697-2025, USA","Department of Molecular Biology and Biochemistry, University of California, Irvine, 1102 NS-2, Irvine, CA 92697-2025, USA","Department of Pharmaceutical Sciences, University of California, Irvine, 1102 NS-2, Irvine, CA 92697-2025, USA"],"raw_orcid":"https://orcid.org/0000-0003-0296-9846","affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of California, Irvine, 1102 NS-2, Irvine, CA 92697-2025, USA; Department of Molecular Biology and Biochemistry, University of California, Irvine, 1102 NS-2, Irvine, CA 92697-2025, USA; Department of Pharmaceutical Sciences, University of California, Irvine, 1102 NS-2, Irvine, CA 92697-2025, USA. Electronic address: gweiss@uci.edu","institution_ids":["https://openalex.org/I204250578"]},{"raw_affiliation_string":"Department of Chemistry, University of California, Irvine, 1102 NS-2, Irvine, CA 92697-2025, USA","institution_ids":["https://openalex.org/I204250578"]},{"raw_affiliation_string":"Department of Molecular Biology and Biochemistry, University of California, Irvine, 1102 NS-2, Irvine, CA 92697-2025, USA","institution_ids":["https://openalex.org/I204250578"]},{"raw_affiliation_string":"Department of Pharmaceutical Sciences, University of California, Irvine, 1102 NS-2, Irvine, CA 92697-2025, USA","institution_ids":["https://openalex.org/I204250578"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5006888208"],"corresponding_institution_ids":["https://openalex.org/I204250578"],"apc_list":{"value":8900,"currency":"USD","value_usd":8900},"apc_paid":{"value":8900,"currency":"USD","value_usd":8900},"fwci":0.7195,"has_fulltext":false,"cited_by_count":55,"citation_normalized_percentile":{"value":0.59808405,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":93,"max":100},"biblio":{"volume":"29","issue":"2","first_page":"177","last_page":"190"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.5273000001907349,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.5273000001907349,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.15440000593662262,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13326","display_name":"Biochemical and Structural Characterization","score":0.09730000048875809,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/protease-engineering","display_name":"protease engineering","score":0.9879999756813049},{"id":"https://openalex.org/keywords/protease-specificity","display_name":"protease specificity","score":0.734000027179718},{"id":"https://openalex.org/keywords/catalytic-efficiency","display_name":"catalytic efficiency","score":0.9810000061988831},{"id":"https://openalex.org/keywords/protease-stability","display_name":"protease stability","score":0.8510000109672546},{"id":"https://openalex.org/keywords/industrial-enzymes","display_name":"industrial enzymes","score":0.7870000004768372},{"id":"https://openalex.org/keywords/proteomics","display_name":"proteomics","score":0.890999972820282},{"id":"https://openalex.org/keywords/protein-sequencing","display_name":"protein sequencing","score":0.9959999918937683},{"id":"https://openalex.org/keywords/synthetic-gene-circuits","display_name":"synthetic gene circuits","score":0.902999997138977},{"id":"https://openalex.org/keywords/protease-inhibitors","display_name":"protease inhibitors","score":0.4300000071525574}],"concepts":[{"id":"https://openalex.org/C182220744","wikidata":"https://www.wikidata.org/wiki/Q7251226","display_name":"Proteases","level":3,"score":0.9682440757751465},{"id":"https://openalex.org/C2776714187","wikidata":"https://www.wikidata.org/wiki/Q212410","display_name":"Protease","level":3,"score":0.6925477981567383},{"id":"https://openalex.org/C147816474","wikidata":"https://www.wikidata.org/wiki/Q169525","display_name":"Protein engineering","level":3,"score":0.47411802411079407},{"id":"https://openalex.org/C183696295","wikidata":"https://www.wikidata.org/wiki/Q2487696","display_name":"Biochemical engineering","level":1,"score":0.4708114564418793},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3802500069141388},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.37664151191711426},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.33702409267425537},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.2939663231372833},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.2869817614555359},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.22368493676185608}],"mesh":[{"descriptor_ui":"D010447","descriptor_name":"Peptide Hydrolases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D010447","descriptor_name":"Peptide Hydrolases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D010447","descriptor_name":"Peptide Hydrolases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D015202","descriptor_name":"Protein Engineering","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.1016/j.chembiol.2021.12.001","doi":"https://doi.org/10.1016/j.chembiol.2021.12.001","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.chembiol.2021.12.001","pdf_url":"http://www.cell.com/article/S2451945621005158/pdf","source":{"id":"https://openalex.org/S4210231961","display_name":"Cell chemical biology","issn_l":"2451-9448","issn":["2451-9448","2451-9456"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cell Chemical Biology","raw_type":"journal-article"},{"id":"pmid:34921772","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/34921772","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cell chemical biology","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:9127713","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/9127713","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Cell Chem Biol","raw_type":"Text"},{"id":"pmh:oai:escholarship.org:ark:/13030/qt7wb0n6cq","doi":null,"is_oa":true,"landing_page_url":"https://escholarship.org/uc/item/7wb0n6cq","pdf_url":null,"source":{"id":"https://openalex.org/S4306400115","display_name":"eScholarship (California Digital Library)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2801248553","host_organization_name":"California Digital Library","host_organization_lineage":["https://openalex.org/I2801248553"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Cell Chemical Biology, vol 29, iss 2","raw_type":"article"}],"best_oa_location":{"id":"doi:10.1016/j.chembiol.2021.12.001","doi":"https://doi.org/10.1016/j.chembiol.2021.12.001","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.chembiol.2021.12.001","pdf_url":"http://www.cell.com/article/S2451945621005158/pdf","source":{"id":"https://openalex.org/S4210231961","display_name":"Cell chemical biology","issn_l":"2451-9448","issn":["2451-9448","2451-9456"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cell Chemical Biology","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2764671288","display_name":null,"funder_award_id":"108201209000","funder_id":"https://openalex.org/F4320332245","funder_display_name":"Forest Laboratories"},{"id":"https://openalex.org/G5574812929","display_name":null,"funder_award_id":"108201209000","funder_id":"https://openalex.org/F4320332585","funder_display_name":"Allergan"},{"id":"https://openalex.org/G6372458318","display_name":"DNA Sequencing Using Single Molecule Electronics","funder_award_id":"R01HG009188","funder_id":"https://openalex.org/F4320337348","funder_display_name":"National Human Genome Research Institute"},{"id":"https://openalex.org/G9000895599","display_name":"A Training Program for Interdisciplinary Cancer Research","funder_award_id":"T32CA009054","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320332245","display_name":"Forest Laboratories","ror":"https://ror.org/03pfqk412"},{"id":"https://openalex.org/F4320332585","display_name":"Allergan","ror":"https://ror.org/00wthd003"},{"id":"https://openalex.org/F4320337348","display_name":"National Human Genome Research Institute","ror":"https://ror.org/00baak391"},{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"}],"has_content":{"pdf":true,"grobid_xml":false},"content_urls":{"pdf":"https://content.openalex.org/works/W4200549490.pdf"},"referenced_works_count":93,"referenced_works":["https://openalex.org/W980708197","https://openalex.org/W1590129082","https://openalex.org/W1954202239","https://openalex.org/W1969136384","https://openalex.org/W1973019272","https://openalex.org/W1976410590","https://openalex.org/W1976548137","https://openalex.org/W1976588667","https://openalex.org/W1980537409","https://openalex.org/W1989689305","https://openalex.org/W1991618419","https://openalex.org/W2001349696","https://openalex.org/W2001931036","https://openalex.org/W2002034161","https://openalex.org/W2003515662","https://openalex.org/W2005767394","https://openalex.org/W2015624242","https://openalex.org/W2015830307","https://openalex.org/W2017328803","https://openalex.org/W2031409259","https://openalex.org/W2034545703","https://openalex.org/W2047893704","https://openalex.org/W2062958450","https://openalex.org/W2064547968","https://openalex.org/W2067080192","https://openalex.org/W2074912245","https://openalex.org/W2108094004","https://openalex.org/W2116556142","https://openalex.org/W2118265845","https://openalex.org/W2135636849","https://openalex.org/W2135900956","https://openalex.org/W2141874760","https://openalex.org/W2148528976","https://openalex.org/W2148614889","https://openalex.org/W2155095892","https://openalex.org/W2157595247","https://openalex.org/W2165026145","https://openalex.org/W2169269559","https://openalex.org/W2172240851","https://openalex.org/W2178366039","https://openalex.org/W2229919188","https://openalex.org/W2238718135","https://openalex.org/W2315398962","https://openalex.org/W2325242266","https://openalex.org/W2341300452","https://openalex.org/W2343251287","https://openalex.org/W2509227587","https://openalex.org/W2559994063","https://openalex.org/W2560862289","https://openalex.org/W2702733083","https://openalex.org/W2725751175","https://openalex.org/W2739415904","https://openalex.org/W2764056337","https://openalex.org/W2765094271","https://openalex.org/W2769449671","https://openalex.org/W2789839292","https://openalex.org/W2789966123","https://openalex.org/W2800333284","https://openalex.org/W2802592819","https://openalex.org/W2804040157","https://openalex.org/W2804327673","https://openalex.org/W2806394880","https://openalex.org/W2807031613","https://openalex.org/W2876540176","https://openalex.org/W2894833434","https://openalex.org/W2900724533","https://openalex.org/W2902888941","https://openalex.org/W2904471651","https://openalex.org/W2908050243","https://openalex.org/W2914019332","https://openalex.org/W2938803199","https://openalex.org/W2952856897","https://openalex.org/W2953992895","https://openalex.org/W2954333117","https://openalex.org/W2964357980","https://openalex.org/W2969304020","https://openalex.org/W2977291005","https://openalex.org/W2982226123","https://openalex.org/W2982304938","https://openalex.org/W2984714480","https://openalex.org/W2994587230","https://openalex.org/W2995692336","https://openalex.org/W3015209591","https://openalex.org/W3041037755","https://openalex.org/W3041920105","https://openalex.org/W3076551716","https://openalex.org/W3088407366","https://openalex.org/W3089876537","https://openalex.org/W3129903208","https://openalex.org/W4237040057","https://openalex.org/W4249558460","https://openalex.org/W4282974318","https://openalex.org/W6777251075"],"related_works":["https://openalex.org/W4410292211","https://openalex.org/W2509227587","https://openalex.org/W2112706163","https://openalex.org/W2422560977","https://openalex.org/W2067080192","https://openalex.org/W4205920351","https://openalex.org/W2480094861","https://openalex.org/W2982571875","https://openalex.org/W2040970646","https://openalex.org/W2910679331"],"abstract_inverted_index":{"Proteases":[0],"cut":[1],"with":[2,21,103],"enviable":[3],"precision":[4],"and":[5,24,44,53,57,72,82,89,113],"regulate":[6],"diverse":[7],"molecular":[8],"events":[9],"in":[10,36,63,109],"biology.":[11],"Such":[12],"qualities":[13],"drive":[14],"a":[15],"seemingly":[16],"inexhaustible":[17],"appetite":[18],"for":[19,98],"proteases":[20,34,48,105],"new":[22,99],"activities":[23],"capabilities.":[25],"Comprising":[26],"25%":[27],"of":[28],"the":[29],"total":[30],"industrial":[31],"enzyme":[32],"market,":[33],"appear":[35],"consumer":[37],"goods,":[38],"such":[39],"as":[40],"detergents,":[41],"textile":[42],"processing,":[43],"numerous":[45],"foods;":[46],"additionally,":[47],"include":[49],"25":[50],"US":[51],"Food":[52],"Drug":[54],"Administration-approved":[55],"medicines":[56],"various":[58],"research":[59],"tools.":[60],"Recent":[61],"advances":[62],"protease":[64,77],"engineering":[65,78],"strategies":[66],"address":[67],"target":[68],"specificity,":[69],"catalytic":[70],"efficiency,":[71],"stability.":[73],"This":[74],"guide":[75],"to":[76,92,106],"surveys":[79],"best":[80],"practices":[81],"emerging":[83],"strategies.":[84],"We":[85],"further":[86],"highlight":[87],"gaps":[88],"flexibilities":[90],"inherent":[91],"each":[93],"system":[94],"that":[95],"suggest":[96],"opportunities":[97],"technology":[100],"development":[101],"along":[102],"engineered":[104],"solve":[107],"challenges":[108],"proteomics,":[110],"protein":[111],"sequencing,":[112],"synthetic":[114],"gene":[115],"circuits.":[116]},"counts_by_year":[{"year":2026,"cited_by_count":14},{"year":2025,"cited_by_count":14},{"year":2024,"cited_by_count":12},{"year":2023,"cited_by_count":13},{"year":2022,"cited_by_count":2}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2021-12-31T00:00:00"}